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Protein

Thiamine thiazole synthase

Gene

THI4

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82Substrate; via amide nitrogenUniRule annotation1
Binding sitei111Substrate; via amide nitrogenUniRule annotation1
Binding sitei176Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei212SubstrateUniRule annotation1
Binding sitei242SubstrateUniRule annotation1
Binding sitei296Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI4UniRule annotation
Ordered Locus Names:AAL137W
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome I

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004158701 – 331Thiamine thiazole synthaseAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2102,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-210 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Proteomic databases

PRIDEiQ75F65

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi33169.AAS50229

Structurei

3D structure databases

ProteinModelPortaliQ75F65
SMRiQ75F65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 104Substrate bindingUniRule annotation2
Regioni306 – 308Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiQ75F65
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q75F65-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQNEHSEFAK MSQTLTNVQE LRLRASTTRH ALSDIVREKD WSDFQFAPIR
60 70 80 90 100
EATVSRAMTT RYFEDLYRYA VSDVVIVGAG SSGLSAAYVL AKNRPDLRIA
110 120 130 140 150
IIEANVAPGG GAWLGGQLFS AMIMRKPTHL FLDELEIPYE DEGDYVVVKH
160 170 180 190 200
AALFTSTVLS KVLQFPNVKL FNATAVEDLV TKPSANGGVT IAGVVTNWTL
210 220 230 240 250
VTMAHDVQSC MDPNVIELEG YKDDGTRDPK KKHGVVLSTT GHDGPFGAFC
260 270 280 290 300
AKRLAALDAQ HAIKGMQSLD MNTAEAGVVK ESGATAGVEY MYFAGMETAT
310 320 330
KKGVSRMGPT FGAMAVSGIK AAEEILRHFA E
Length:331
Mass (Da):35,873
Last modified:July 5, 2004 - v1
Checksum:i7C26467D9974FF34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016814 Genomic DNA Translation: AAS50229.1
RefSeqiNP_982405.1, NM_207758.1

Genome annotation databases

EnsemblFungiiAAS50229; AAS50229; AGOS_AAL137W
GeneIDi4618464
KEGGiago:AGOS_AAL137W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016814 Genomic DNA Translation: AAS50229.1
RefSeqiNP_982405.1, NM_207758.1

3D structure databases

ProteinModelPortaliQ75F65
SMRiQ75F65
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi33169.AAS50229

Proteomic databases

PRIDEiQ75F65

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAS50229; AAS50229; AGOS_AAL137W
GeneIDi4618464
KEGGiago:AGOS_AAL137W

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiQ75F65
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_ASHGO
AccessioniPrimary (citable) accession number: Q75F65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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