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Protein

Delta-aminolevulinic acid dehydratase

Gene

HEM2

Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per monomer.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (HEM2)
  2. Porphobilinogen deaminase (HEM3)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (AGOS_AGL210C)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Zinc; catalyticBy similarity1
Metal bindingi136Zinc; catalyticBy similarity1
Metal bindingi144Zinc; catalyticBy similarity1
Active sitei211Schiff-base intermediate with substrateBy similarity1
Binding sitei221Substrate 1By similarity1
Binding sitei233Substrate 1By similarity1
Active sitei264Schiff-base intermediate with substrateBy similarity1
Binding sitei291Substrate 2By similarity1
Binding sitei330Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEM2
Ordered Locus Names:AGR015C
OrganismiAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Taxonomic identifieri284811 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeEremothecium
Proteomesi
  • UP000000591 Componenti: Chromosome VII

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001405311 – 340Delta-aminolevulinic acid dehydrataseAdd BLAST340

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi33169.AAS54504

Structurei

3D structure databases

ProteinModelPortaliQ750E0
SMRiQ750E0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

HOGENOMiHOG000020323
InParanoidiQ750E0
KOiK01698
OMAiYQMDYAN
OrthoDBiEOG092C32OH

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR030656 ALAD_AS
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PIRSFiPIRSF001415 Porphbilin_synth, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit
PROSITEiView protein in PROSITE
PS00169 D_ALA_DEHYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q750E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHTAEFLDT HQTQISSILS GGYNHPLLRE WQNERQLAKN MFIFPLFVSD
60 70 80 90 100
MPDEDQPIES LPNIRRFGVN KLAGYLKPLV AKGLRAVLLF GVPMKPNSKD
110 120 130 140 150
EFGSAADDPE GPVIQAIKLL RAEFPELYIL CDVCLCEYTS HGHCGVLYDD
160 170 180 190 200
GSINREQSVR RLAAVAVNYA KAGAHSVAPS DMIDGRIRDI KLGLLAAGLA
210 220 230 240 250
HKTFVMSYAA KFSGNLYGPF RDAACSAPSQ GDRKCYQLPS GGRGLARRAL
260 270 280 290 300
RRDLDEGADG IIVKPSTFYL DIMADASEIA KDVPICAYHV SGEYAMLHAA
310 320 330 340
AKAGVVDFKS IAFESHQGFL RAGARLIISY MTPEFLDWLS
Length:340
Mass (Da):37,331
Last modified:July 5, 2004 - v1
Checksum:iEA5FAB60A7562999
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016820 Genomic DNA Translation: AAS54504.1
RefSeqiNP_986680.1, NM_211742.1

Genome annotation databases

EnsemblFungiiAAS54504; AAS54504; AGOS_AGR015C
GeneIDi4622979
KEGGiago:AGOS_AGR015C

Similar proteinsi

Entry informationi

Entry nameiHEM2_ASHGO
AccessioniPrimary (citable) accession number: Q750E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 5, 2004
Last modified: July 5, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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