UniProtKB - Q732M9 (TRMD_BACC1)
Protein
tRNA (guanine-N(1)-)-methyltransferase
Gene
trmD
Organism
Bacillus cereus (strain ATCC 10987 / NRS 248)
Status
Functioni
Specifically methylates guanosine-37 in various tRNAs.UniRule annotation
Catalytic activityi
- guanosine37 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.228UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 113 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | BCER222523:G1G0J-3844-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)Alternative name(s): M1G-methyltransferaseUniRule annotation tRNA [GM37] methyltransferaseUniRule annotation |
Gene namesi | Name:trmDUniRule annotation Ordered Locus Names:BCE_3883 |
Organismi | Bacillus cereus (strain ATCC 10987 / NRS 248) |
Taxonomic identifieri | 222523 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060320 | 1 – 244 | tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST | 244 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 133 – 138 | S-adenosyl-L-methionine bindingUniRule annotation | 6 |
Sequence similaritiesi
Belongs to the RNA methyltransferase TrmD family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_047363_0_1_9 |
OMAi | ILCGHYK |
Family and domain databases
CDDi | cd18080, TrmD-like, 1 hit |
Gene3Di | 1.10.1270.20, 1 hit 3.40.1280.10, 1 hit |
HAMAPi | MF_00605, TrmD, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR002649, tRNA_m1G_MeTrfase_bac IPR023148, tRNA_m1G_MeTrfase_C_sf IPR029026, tRNA_m1G_MTases_N IPR016009, tRNA_MeTrfase_TRMD/TRM10 |
PANTHERi | PTHR46417, PTHR46417, 1 hit |
Pfami | View protein in Pfam PF01746, tRNA_m1G_MT, 1 hit |
PIRSFi | PIRSF000386, tRNA_mtase, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00088, trmD, 1 hit |
i Sequence
Sequence statusi: Complete.
Q732M9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIDILTLFP DMFTGVFGSS ILKKAQEKEA VELRVVNFRD YTTSKHNSVD
60 70 80 90 100
DYPYGGGAGM VLTPQPIFDA VEDLTKETER KPRVVLMCPQ GERFTQKKAE
110 120 130 140 150
ELAEEEHLIF VCGHYEGYDE RIREHLVTDE ISIGDYVLTG GELASMVITD
160 170 180 190 200
SVVRLLPGVL GNHASQVEDS FSTGLLEHPH YTRPADFRGM KVPDVLMSGN
210 220 230 240
HKNIDEWRHK ESLRRTYTRR PDLLEERELS KQEKKWLEQI KEGK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017194 Genomic DNA Translation: AAS42788.1 |
RefSeqi | WP_000686892.1, NC_003909.8 |
Genome annotation databases
EnsemblBacteriai | AAS42788; AAS42788; BCE_3883 |
GeneIDi | 59155662 |
KEGGi | bca:BCE_3883 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017194 Genomic DNA Translation: AAS42788.1 |
RefSeqi | WP_000686892.1, NC_003909.8 |
3D structure databases
SMRi | Q732M9 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AAS42788; AAS42788; BCE_3883 |
GeneIDi | 59155662 |
KEGGi | bca:BCE_3883 |
Phylogenomic databases
HOGENOMi | CLU_047363_0_1_9 |
OMAi | ILCGHYK |
Enzyme and pathway databases
BioCyci | BCER222523:G1G0J-3844-MONOMER |
Family and domain databases
CDDi | cd18080, TrmD-like, 1 hit |
Gene3Di | 1.10.1270.20, 1 hit 3.40.1280.10, 1 hit |
HAMAPi | MF_00605, TrmD, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR002649, tRNA_m1G_MeTrfase_bac IPR023148, tRNA_m1G_MeTrfase_C_sf IPR029026, tRNA_m1G_MTases_N IPR016009, tRNA_MeTrfase_TRMD/TRM10 |
PANTHERi | PTHR46417, PTHR46417, 1 hit |
Pfami | View protein in Pfam PF01746, tRNA_m1G_MT, 1 hit |
PIRSFi | PIRSF000386, tRNA_mtase, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00088, trmD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMD_BACC1 | |
Accessioni | Q732M9Primary (citable) accession number: Q732M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |