UniProtKB - Q72K16 (PAAK_THET2)
Protein
Phenylacetate-coenzyme A ligase
Gene
TT_C0602
Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Functioni
Catalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA). Involved in the phenylalanine metabolism.1 Publication
Catalytic activityi
- EC:6.2.1.301 Publication
Kineticsi
- KM=6 µM for ATP (at 50 degrees Celsius and pH 7.5)1 Publication
- KM=30 µM for CoA (at 50 degrees Celsius and pH 7.5)1 Publication
- KM=50 µM for PA (at 50 degrees Celsius and pH 7.5)1 Publication
Temperature dependencei
Optimum temperature is 75 degrees Celsius. The enzyme is heat stable.1 Publication
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB
- phenylacetate-CoA ligase activity Source: UniProtKB
GO - Biological processi
- phenylacetate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.2.1.30, 2305 |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | Recommended name: Phenylacetate-coenzyme A ligase (EC:6.2.1.30)Alternative name(s): Phenylacetyl-CoA ligase Short name: PA-CoA ligase |
Gene namesi | Ordered Locus Names:TT_C0602 |
Organismi | Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) |
Taxonomic identifieri | 262724 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000416774 | 1 – 445 | Phenylacetate-coenzyme A ligaseAdd BLAST | 445 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 262724.TT_C0602 |
Family & Domainsi
Sequence similaritiesi
Belongs to the phenylacetyl-CoA ligase family.Curated
Phylogenomic databases
eggNOGi | COG1541, Bacteria |
HOGENOMi | CLU_035301_1_1_0 |
OMAi | TGRCDDM |
Family and domain databases
CDDi | cd05913, PaaK, 1 hit |
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR028154, AMP-dep_Lig_C IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR011880, PA_CoA_ligase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF14535, AMP-binding_C_2, 1 hit |
PIRSFi | PIRSF006444, PaaK, 1 hit |
i Sequence
Sequence statusi: Complete.
Q72K16-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMYQPELETL PREKLRALQE ERLKRLVAYV YERVPFYRRL LDEAGVDPKG
60 70 80 90 100
FRGLEDLPRI PFTKKTDLRD HYPFGLFAVP REEVARVHAS SGTTGKPTVV
110 120 130 140 150
GYTKNDLKVF AEVVARSLAA AGARPGMMLH NAYGYGLFTG GLGLHGGAEA
160 170 180 190 200
LGMTVVPVSG GMTERQVMLI QDFRPEVISC TPSYAQTLAE EFRKRGVSPE
210 220 230 240 250
ELSLEYAVLG AEPWTEAIRK QVDEGLGVKS TNIYGLSEII GPGVSNECVE
260 270 280 290 300
ERQGSHIWED HFLPEVVDPD TGEPLPEGKV GVLVFTTLTK EAMPLLRYWT
310 320 330 340 350
GDLTFLTYEA CTCGRTHVRM GPILGRTDDM LIIRGVNVYP TQVEAVLLAI
360 370 380 390 400
PEVVPHYQIV VRREGTLDEA ELKVEVSEPF FREIGQEVLS DEVVEADHRL
410 420 430 440
HALRERIARK IKDNVGVTLK VTLLPPGQAP RSEGGKLRRV LDLRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017221 Genomic DNA Translation: AAS80950.1 |
Genome annotation databases
EnsemblBacteriai | AAS80950; AAS80950; TT_C0602 |
KEGGi | tth:TT_C0602 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017221 Genomic DNA Translation: AAS80950.1 |
3D structure databases
SMRi | Q72K16 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 262724.TT_C0602 |
Genome annotation databases
EnsemblBacteriai | AAS80950; AAS80950; TT_C0602 |
KEGGi | tth:TT_C0602 |
Phylogenomic databases
eggNOGi | COG1541, Bacteria |
HOGENOMi | CLU_035301_1_1_0 |
OMAi | TGRCDDM |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BRENDAi | 6.2.1.30, 2305 |
Family and domain databases
CDDi | cd05913, PaaK, 1 hit |
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR028154, AMP-dep_Lig_C IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR011880, PA_CoA_ligase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF14535, AMP-binding_C_2, 1 hit |
PIRSFi | PIRSF006444, PaaK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAK_THET2 | |
Accessioni | Q72K16Primary (citable) accession number: Q72K16 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2012 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families