UniProtKB - Q728Y4 (LPXK_DESVH)
Protein
Tetraacyldisaccharide 4'-kinase
Gene
lpxK
Organism
Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)
Status
Functioni
Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation
Catalytic activityi
- ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation EC:2.7.1.130
: lipid IV(A) biosynthesis Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationProteins known to be involved in the 6 steps of the subpathway in this organism are:
- Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
- UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
- no protein annotated in this organism
- no protein annotated in this organism
- Lipid-A-disaccharide synthase (lpxB)
- Tetraacyldisaccharide 4'-kinase (lpxK)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 53 – 60 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- tetraacyldisaccharide 4'-kinase activity Source: UniProtKB-UniRule
GO - Biological processi
- lipid A biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-2537-MONOMER |
UniPathwayi | UPA00359;UER00482 |
Names & Taxonomyi
Protein namesi | Recommended name: Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)Alternative name(s): Lipid A 4'-kinaseUniRule annotation |
Gene namesi | Name:lpxKUniRule annotation Ordered Locus Names:DVU_2468 |
Organismi | Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303) |
Taxonomic identifieri | 882 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000340831 | 1 – 354 | Tetraacyldisaccharide 4'-kinaseAdd BLAST | 354 |
Proteomic databases
PaxDbi | Q728Y4 |
Family & Domainsi
Sequence similaritiesi
Belongs to the LpxK family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1663, Bacteria |
HOGENOMi | CLU_038816_6_1_7 |
OMAi | MDDGFQN |
PhylomeDBi | Q728Y4 |
Family and domain databases
HAMAPi | MF_00409, LpxK, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003758, Tetraacyldisaccharide_4-kinase |
PANTHERi | PTHR42724, PTHR42724, 1 hit |
Pfami | View protein in Pfam PF02606, LpxK, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00682, lpxK, 1 hit |
i Sequence
Sequence statusi: Complete.
Q728Y4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHVAALQNHL APLLVPCSRV YAACMAVRRR FWESPMSPAF RPSRPVVSVG
60 70 80 90 100
NIAWGGTGKT PLVDWLLHWA GTRGLNPAVL TRGYGAKPPT VPFLVGSQHT
110 120 130 140 150
AEEAGDEPLM LARRNPYAAV LVDPVRRRAG RWAEHELRPH FYLLDDGMQH
160 170 180 190 200
LAVRRDLDLV VLRPDDVLDQ WGRVLPAGSW REGASALKSA TAFFVKSSPE
210 220 230 240 250
VFEALAPVLE ERLAPYGVPV FSFWLRPSGL LRVGGNEQRP HFDGAPYVLV
260 270 280 290 300
SGVGGPGQVG ETATRYFGYA PVRHRVFPDH HPYGPDDVRS LAQEGAQLVC
310 320 330 340 350
TPKDAVKLER FAGLDLWTFD LQTVFGPAIG TAAPFPQWWD ERWARLARER
AGTS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS96940.1 |
RefSeqi | WP_010939738.1, NC_002937.3 YP_011680.1, NC_002937.3 |
Genome annotation databases
EnsemblBacteriai | AAS96940; AAS96940; DVU_2468 |
KEGGi | dvu:DVU2468 |
PATRICi | fig|882.5.peg.2231 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS96940.1 |
RefSeqi | WP_010939738.1, NC_002937.3 YP_011680.1, NC_002937.3 |
3D structure databases
SMRi | Q728Y4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 882.DVU_2468 |
Proteomic databases
PaxDbi | Q728Y4 |
Genome annotation databases
EnsemblBacteriai | AAS96940; AAS96940; DVU_2468 |
KEGGi | dvu:DVU2468 |
PATRICi | fig|882.5.peg.2231 |
Phylogenomic databases
eggNOGi | COG1663, Bacteria |
HOGENOMi | CLU_038816_6_1_7 |
OMAi | MDDGFQN |
PhylomeDBi | Q728Y4 |
Enzyme and pathway databases
UniPathwayi | UPA00359;UER00482 |
BioCyci | DVUL882:G1GT1-2537-MONOMER |
Family and domain databases
HAMAPi | MF_00409, LpxK, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003758, Tetraacyldisaccharide_4-kinase |
PANTHERi | PTHR42724, PTHR42724, 1 hit |
Pfami | View protein in Pfam PF02606, LpxK, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00682, lpxK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPXK_DESVH | |
Accessioni | Q728Y4Primary (citable) accession number: Q728Y4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families