UniProtKB - Q727N1 (ISLA_DESVH)
Protein
Isethionate sulfite-lyase
Gene
iseG
Organism
Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)
Status
Functioni
Involved in an anaerobic respiration pathway that converts the sulfonate isethionate (2-hydroxyethanesulfonate) to ammonia, acetate and sulfide. Catalyzes the radical-mediated C-S bond cleavage of isethionate (2-hydroxyethanesulfonate) to form sulfite and acetaldehyde. Shows no activity with taurine or ethanolamine as substrates.1 Publication
Catalytic activityi
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
kcat is 91.6 sec(-1).1 Publication
- KM=44.8 mM for 2-hydroxyethane-1-sulfonate1 Publication
: alkanesulfonate degradation Pathwayi
This protein is involved in the pathway alkanesulfonate degradation, which is part of Organosulfur degradation.1 PublicationView all proteins of this organism that are known to be involved in the pathway alkanesulfonate degradation and in Organosulfur degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 187 | 2-hydroxyethane-1-sulfonate1 Publication | 1 | |
Binding sitei | 191 | 2-hydroxyethane-1-sulfonate1 Publication | 1 | |
Active sitei | 466 | Cysteine radical intermediate1 Publication | 1 | |
Active sitei | 468 | Proton acceptor1 Publication | 1 | |
Binding sitei | 676 | 2-hydroxyethane-1-sulfonate1 Publication | 1 |
GO - Molecular functioni
- lyase activity Source: UniProtKB-KW
- transferase activity Source: UniProtKB-KW
GO - Biological processi
- alkanesulfonate catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase, Transferase |
Ligand | Pyruvate |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-2900-MONOMER |
UniPathwayi | UPA00338 |
Names & Taxonomyi
Protein namesi | Recommended name: Isethionate sulfite-lyase1 Publication (EC:4.4.1.-1 Publication)Alternative name(s): C-S lyase IseG1 Publication Glycyl radical enzyme IseG1 Publication Short name: GRE IseG1 Publication |
Gene namesi | Name:iseG1 Publication Ordered Locus Names:DVU_2824Imported |
Organismi | Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303) |
Taxonomic identifieri | 882 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000450944 | 1 – 828 | Isethionate sulfite-lyaseAdd BLAST | 828 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 803 | Glycine radicalPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Requires the activating protein IseH to generate the key active site glycyl radical on Gly-803 that is involved in catalysis.1 Publication
Keywords - PTMi
Organic radicalProteomic databases
PaxDbi | Q727N1 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 882.DVU_2824 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q727N1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 698 | PFLPROSITE-ProRule annotationAdd BLAST | 669 | |
Domaini | 705 – 828 | Glycine radicalPROSITE-ProRule annotationAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 466 – 468 | 2-hydroxyethane-1-sulfonate binding1 Publication | 3 |
Sequence similaritiesi
Belongs to the glycyl radical enzyme (GRE) family.Curated
Phylogenomic databases
eggNOGi | ENOG4105C6N, Bacteria COG1882, LUCA |
HOGENOMi | CLU_009096_0_1_7 |
OMAi | HVQYNIV |
PhylomeDBi | Q727N1 |
Family and domain databases
InterProi | View protein in InterPro IPR019777, Form_AcTrfase_GR_CS IPR001150, Gly_radical IPR004184, PFL_dom |
Pfami | View protein in Pfam PF01228, Gly_radical, 1 hit PF02901, PFL-like, 1 hit |
PROSITEi | View protein in PROSITE PS00850, GLY_RADICAL_1, 1 hit PS51149, GLY_RADICAL_2, 1 hit PS51554, PFL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q727N1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQCCNQLSPH EQRLQDKIEG KVDRYRATHE RVFTILESFD NTRPRIDVER
60 70 80 90 100
AKYFTESMKA TEGQPLPLRW AKALMHIAEN MTVYIDDHQL ICGRAGYQGR
110 120 130 140 150
YGVLYPELDG DFLGTAIEDL PNRAESPFAI TPEDARVVVE EIAPFWKGKT
160 170 180 190 200
YHEALNLALP ADVHKLTYDD PQGLMSRFIV NETSSFRSSI QWVHDYEKVL
210 220 230 240 250
KRGFRSIKEE ALEKIAALDP MSPCDNVEKR PFLEAIVIVC DAIILWAKRH
260 270 280 290 300
AKLAAELAAK ETDPTRKREL ETMAEICAWV PENPARTFHE AVQAQWFTQV
310 320 330 340 350
FSRIEQKTGT IVSNGRMDQY FWPFYEKDLA EGRITEDSAL ELLECMWVGM
360 370 380 390 400
AQYVDLYISP TGGAFNEGYA HWEAVTIGGQ TPEGRDATND LTYLFLKSKR
410 420 430 440 450
EFPLHYPDLA ARIHSRSPER YLWEVAETIK DGSGFPKLIN DEEVVPLYVS
460 470 480 490 500
KGATFAEALD YAVSGCTEAR MPNRDTYTSG GAYINFAAAL EMVLYNGKML
510 520 530 540 550
KYGDTDLGAH TGDPCEFKTW EEFWNAYVTQ HHLFLKTAFV QQHIINNLRA
560 570 580 590 600
RHFAQPMGSS LHDLCMKHCL DLHTPQIPEG INLGYFEYMG FGTVVDSLSA
610 620 630 640 650
IKKLVFEDKK LTMGELIEAL KCNFEGKEDI QQLLKSAPCY GNNDDYADSI
660 670 680 690 700
ARDIDALSVK YGRRYSPELG MHNDVRYVPF TSHVPFGRVV SATPNGRKAW
710 720 730 740 750
SALSDGSSAS HGADVNGPTA ILQSNFNSKN YGMRDRAARM LNIKFTPKCV
760 770 780 790 800
EGEEGSQKLV SFIRTFCDLK LWHVQFNVIN KETLLAAQRD PEKYRNLIVR
810 820
IAGYSAYFVD LSPDLQNDLI ARTGHDVM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS97296.1 |
RefSeqi | WP_010940090.1, NZ_CABHLV010000001.1 YP_012036.1, NC_002937.3 |
Genome annotation databases
EnsemblBacteriai | AAS97296; AAS97296; DVU_2824 |
KEGGi | dvu:DVU2824 |
PATRICi | fig|882.5.peg.2552 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS97296.1 |
RefSeqi | WP_010940090.1, NZ_CABHLV010000001.1 YP_012036.1, NC_002937.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5YMR | X-ray | 2.40 | A/B/C/D | 24-828 | [»] | |
SMRi | Q727N1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 882.DVU_2824 |
Proteomic databases
PaxDbi | Q727N1 |
Genome annotation databases
EnsemblBacteriai | AAS97296; AAS97296; DVU_2824 |
KEGGi | dvu:DVU2824 |
PATRICi | fig|882.5.peg.2552 |
Phylogenomic databases
eggNOGi | ENOG4105C6N, Bacteria COG1882, LUCA |
HOGENOMi | CLU_009096_0_1_7 |
OMAi | HVQYNIV |
PhylomeDBi | Q727N1 |
Enzyme and pathway databases
UniPathwayi | UPA00338 |
BioCyci | DVUL882:G1GT1-2900-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR019777, Form_AcTrfase_GR_CS IPR001150, Gly_radical IPR004184, PFL_dom |
Pfami | View protein in Pfam PF01228, Gly_radical, 1 hit PF02901, PFL-like, 1 hit |
PROSITEi | View protein in PROSITE PS00850, GLY_RADICAL_1, 1 hit PS51149, GLY_RADICAL_2, 1 hit PS51554, PFL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ISLA_DESVH | |
Accessioni | Q727N1Primary (citable) accession number: Q727N1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 7, 2020 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families