UniProtKB - Q726F7 (RIMO_DESVH)
Protein
Ribosomal protein S12 methylthiotransferase RimO
Gene
rimO
Organism
Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)
Status
Functioni
Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation
Catalytic activityi
- [sulfur carrier]-SH + AH2 + L-aspartate89-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate89-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteineUniRule annotationEC:2.8.4.4UniRule annotation
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 11 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 46 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 81 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 145 | Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 149 | Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 152 | Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- protein methylthiotransferase activity Source: UniProtKB-EC
GO - Biological processi
- peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid Source: UniProtKB-UniRule
- tRNA modification Source: InterPro
Keywordsi
Molecular function | Transferase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-3242-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation)Short name: S12 MTTaseUniRule annotation Short name: S12 methylthiotransferaseUniRule annotation Alternative name(s): Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation Ribosome maturation factor RimOUniRule annotation |
Gene namesi | Name:rimOUniRule annotation Ordered Locus Names:DVU_3151 |
Organismi | Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303) |
Taxonomic identifieri | 882 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000374808 | 1 – 430 | Ribosomal protein S12 methylthiotransferase RimOAdd BLAST | 430 |
Proteomic databases
PaxDbi | Q726F7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 119 | MTTase N-terminalUniRule annotationAdd BLAST | 118 | |
Domaini | 131 – 361 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 231 | |
Domaini | 364 – 430 | TRAMUniRule annotationAdd BLAST | 67 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0621, Bacteria |
HOGENOMi | CLU_018697_0_1_7 |
OMAi | CLSERYK |
PhylomeDBi | Q726F7 |
Family and domain databases
Gene3Di | 3.40.50.12160, 1 hit 3.80.30.20, 1 hit |
HAMAPi | MF_01865, MTTase_RimO, 1 hit |
InterProi | View protein in InterPro IPR006638, Elp3/MiaB/NifB IPR005839, Methylthiotransferase IPR020612, Methylthiotransferase_CS IPR013848, Methylthiotransferase_N IPR038135, Methylthiotransferase_N_sf IPR005840, Ribosomal_S12_MeSTrfase_RimO IPR041582, RimO_TRAM IPR007197, rSAM IPR023404, rSAM_horseshoe IPR002792, TRAM_dom |
PANTHERi | PTHR43837, PTHR43837, 1 hit |
Pfami | View protein in Pfam PF04055, Radical_SAM, 1 hit PF18693, TRAM_2, 1 hit PF00919, UPF0004, 1 hit |
SFLDi | SFLDG01061, methylthiotransferase, 1 hit SFLDF00274, ribosomal_protein_S12_methylth, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
TIGRFAMsi | TIGR00089, TIGR00089, 1 hit TIGR01125, TIGR01125, 1 hit |
PROSITEi | View protein in PROSITE PS51449, MTTASE_N, 1 hit PS01278, MTTASE_RADICAL, 1 hit PS51918, RADICAL_SAM, 1 hit PS50926, TRAM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q726F7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISVYSISLG CPKNRVDTEH LLGSLGVAVQ PVEHLSRADV VLINTCGFIL
60 70 80 90 100
PAVEESVRTI VETIDDLSGL RKRPLLAVAG CLVGRYGAKE LASELPEVDV
110 120 130 140 150
WLPNQDITAW PAMLAHALKL EGAVTPGRLL STGPSYAWLK ISDGCRHNCS
160 170 180 190 200
FCTIPSIRGG HRSTPADVLE REARDLVAQG VRELVLVAQD VTAWGEDIGA
210 220 230 240 250
PHGLATLLER LLPVPGLARL RLMYLYPAGL TRELLGFMRD AGAPLVPYFD
260 270 280 290 300
VPLQHAHPDI LSRMGRPFAR DPRRVVERVR DFFPDAALRT SLIVGFPGET
310 320 330 340 350
DEHYAALTSF VEETRFTHMG VFAYRAEEGT PAAEMPEQVE DRVKEWRRDA
360 370 380 390 400
LMEVQAEISE ELLAVHEGTR QQVLVDAPHE EWPGLHTGRT WFQAPEIDGI
410 420 430
TYVSGPGVEP GALVEADIVE TRTYDLVALA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS97621.1 |
RefSeqi | WP_010940409.1, NZ_CABHLV010000001.1 YP_012361.1, NC_002937.3 |
Genome annotation databases
EnsemblBacteriai | AAS97621; AAS97621; DVU_3151 |
KEGGi | dvu:DVU3151 |
PATRICi | fig|882.5.peg.2857 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017285 Genomic DNA Translation: AAS97621.1 |
RefSeqi | WP_010940409.1, NZ_CABHLV010000001.1 YP_012361.1, NC_002937.3 |
3D structure databases
SMRi | Q726F7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 882.DVU_3151 |
Proteomic databases
PaxDbi | Q726F7 |
Genome annotation databases
EnsemblBacteriai | AAS97621; AAS97621; DVU_3151 |
KEGGi | dvu:DVU3151 |
PATRICi | fig|882.5.peg.2857 |
Phylogenomic databases
eggNOGi | COG0621, Bacteria |
HOGENOMi | CLU_018697_0_1_7 |
OMAi | CLSERYK |
PhylomeDBi | Q726F7 |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-3242-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.12160, 1 hit 3.80.30.20, 1 hit |
HAMAPi | MF_01865, MTTase_RimO, 1 hit |
InterProi | View protein in InterPro IPR006638, Elp3/MiaB/NifB IPR005839, Methylthiotransferase IPR020612, Methylthiotransferase_CS IPR013848, Methylthiotransferase_N IPR038135, Methylthiotransferase_N_sf IPR005840, Ribosomal_S12_MeSTrfase_RimO IPR041582, RimO_TRAM IPR007197, rSAM IPR023404, rSAM_horseshoe IPR002792, TRAM_dom |
PANTHERi | PTHR43837, PTHR43837, 1 hit |
Pfami | View protein in Pfam PF04055, Radical_SAM, 1 hit PF18693, TRAM_2, 1 hit PF00919, UPF0004, 1 hit |
SFLDi | SFLDG01061, methylthiotransferase, 1 hit SFLDF00274, ribosomal_protein_S12_methylth, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
TIGRFAMsi | TIGR00089, TIGR00089, 1 hit TIGR01125, TIGR01125, 1 hit |
PROSITEi | View protein in PROSITE PS51449, MTTASE_N, 1 hit PS01278, MTTASE_RADICAL, 1 hit PS51918, RADICAL_SAM, 1 hit PS50926, TRAM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIMO_DESVH | |
Accessioni | Q726F7Primary (citable) accession number: Q726F7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 26, 2009 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families