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UniProtKB - Q71ZR3 (PFLA_LISMF)
Protein
Pyruvate formate-lyase-activating enzyme
Gene
pflA
Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Functioni
Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.
By similarityCatalytic activityi
- glycyl-[formate C-acetyltransferase] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[formate C-acetyltransferase] + H+ + L-methionine + semiquinone [flavodoxin]EC:1.97.1.4
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 31 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Metal bindingi | 35 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Metal bindingi | 38 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Binding sitei | 80 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 208 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)Short name: PFL-activating enzyme |
Gene namesi | Name:pflA Ordered Locus Names:LMOf2365_1426 |
Organismi | Listeria monocytogenes serotype 4b (strain F2365) |
Taxonomic identifieri | 265669 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000200531 | 1 – 248 | Pyruvate formate-lyase-activating enzymeAdd BLAST | 248 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 248 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 232 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 37 – 39 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 135 – 137 | S-adenosyl-L-methionine bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the organic radical-activating enzymes family.Curated
Phylogenomic databases
HOGENOMi | CLU_058969_1_1_9 |
OMAi | IGVPNKR |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR034457, Organic_radical-activating IPR012839, Organic_radical_activase IPR012838, PFL1_activating IPR034465, Pyruvate_for-lyase_activase IPR001989, Radical_activat_CS IPR007197, rSAM |
PANTHERi | PTHR30352, PTHR30352, 1 hit |
Pfami | View protein in Pfam PF04055, Radical_SAM, 1 hit |
PIRSFi | PIRSF000371, PFL_act_enz, 1 hit |
SFLDi | SFLDF00278, pyruvate_formate-lyase_activas, 1 hit |
TIGRFAMsi | TIGR02493, PFLA, 1 hit |
PROSITEi | View protein in PROSITE PS01087, RADICAL_ACTIVATING, 1 hit PS51918, RADICAL_SAM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q71ZR3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT
60 70 80 90 100
ERSAQDVFDE AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG
110 120 130 140 150
VHTTIDSCGG CFTRDPEFIE KLDRLMEVTD LILLDIKQIN PEKHLKLTTK
160 170 180 190 200
SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT DDPEDLTKLH EFIQTLPNVK
210 220 230 240
QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK ILETSSYK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017262 Genomic DNA Translation: AAT04201.1 |
RefSeqi | WP_003721912.1, NC_002973.6 |
Genome annotation databases
GeneIDi | 61170461 |
KEGGi | lmf:LMOf2365_1426 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017262 Genomic DNA Translation: AAT04201.1 |
RefSeqi | WP_003721912.1, NC_002973.6 |
3D structure databases
AlphaFoldDBi | Q71ZR3 |
SMRi | Q71ZR3 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 61170461 |
KEGGi | lmf:LMOf2365_1426 |
Phylogenomic databases
HOGENOMi | CLU_058969_1_1_9 |
OMAi | IGVPNKR |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR034457, Organic_radical-activating IPR012839, Organic_radical_activase IPR012838, PFL1_activating IPR034465, Pyruvate_for-lyase_activase IPR001989, Radical_activat_CS IPR007197, rSAM |
PANTHERi | PTHR30352, PTHR30352, 1 hit |
Pfami | View protein in Pfam PF04055, Radical_SAM, 1 hit |
PIRSFi | PIRSF000371, PFL_act_enz, 1 hit |
SFLDi | SFLDF00278, pyruvate_formate-lyase_activas, 1 hit |
TIGRFAMsi | TIGR02493, PFLA, 1 hit |
PROSITEi | View protein in PROSITE PS01087, RADICAL_ACTIVATING, 1 hit PS51918, RADICAL_SAM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PFLA_LISMF | |
Accessioni | Q71ZR3Primary (citable) accession number: Q71ZR3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |