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Entry version 129 (07 Oct 2020)
Sequence version 2 (02 Oct 2007)
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Protein

Mediator of RNA polymerase II transcription subunit 25

Gene

MED25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.3 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

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PathwayCommonsi
Q71SY5

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-1989781, PPARA activates gene expression
R-HSA-212436, Generic Transcription Pathway
R-HSA-381340, Transcriptional regulation of white adipocyte differentiation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 25
Alternative name(s):
Activator interaction domain-containing protein 1
Activator-recruited cofactor 92 kDa component
Short name:
ARC92
Mediator complex subunit 25
p78
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MED25
Synonyms:ACID1, ARC92, PTOV2
ORF Names:TCBAP0758
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Eukaryotic Pathogen Database Resources

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EuPathDBi
HostDB:ENSG00000104973.14

Human Gene Nomenclature Database

More...
HGNCi
HGNC:28845, MED25

Online Mendelian Inheritance in Man (OMIM)

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MIMi
610197, gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q71SY5

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Charcot-Marie-Tooth disease 2B2 (CMT2B2)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_063521335A → V in CMT2B2. 1 PublicationCorresponds to variant dbSNP:rs145770066EnsemblClinVar.1
Basel-Vanagaite-Smirin-Yosef syndrome (BVSYS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive syndrome characterized by eye, brain, cardiac and palatal abnormalities as well as growth retardation, microcephaly and severe intellectual disability.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07394939Y → C in BVSYS; the mutation impairs interaction with the Mediator complex. 1 PublicationCorresponds to variant dbSNP:rs794729668EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi646L → A: Abrogates interaction with RARA. 1 Publication1
Mutagenesisi649 – 650LL → AA: Abrogates interaction with RARA. 1 Publication2

Keywords - Diseasei

Charcot-Marie-Tooth disease, Disease mutation, Mental retardation, Neurodegeneration, Neuropathy

Organism-specific databases

DisGeNET

More...
DisGeNETi
81857

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...
GeneReviewsi
MED25

MalaCards human disease database

More...
MalaCardsi
MED25
MIMi605589, phenotype
616449, phenotype

Open Targets

More...
OpenTargetsi
ENSG00000104973

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...
Orphaneti
88616, Autosomal recessive non-syndromic intellectual disability
101101, Charcot-Marie-Tooth disease type 2B2
464738, Congenital cataract-microcephaly-nevus flammeus simplex-severe intellectual disability syndrome

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA134984839

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

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Pharosi
Q71SY5, Tbio

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
MED25

Domain mapping of disease mutations (DMDM)

More...
DMDMi
158706143

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003049521 – 747Mediator of RNA polymerase II transcription subunit 25Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei725Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Methylation

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q71SY5

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q71SY5

MaxQB - The MaxQuant DataBase

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MaxQBi
Q71SY5

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q71SY5

PeptideAtlas

More...
PeptideAtlasi
Q71SY5

PRoteomics IDEntifications database

More...
PRIDEi
Q71SY5

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
68625 [Q71SY5-1]
68628 [Q71SY5-4]
68629 [Q71SY5-5]
7538

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q71SY5

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q71SY5

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000104973, Expressed in oocyte and 218 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q71SY5, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q71SY5, HS

Organism-specific databases

Human Protein Atlas

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HPAi
ENSG00000104973, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

Interacts with CREBBP.

Interacts with ESR1, GR, RARA, RXRA and THRB in a ligand-dependent fashion. Binds the Herpes simplex virus activator VP16.

5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
123607, 72 interactors

ComplexPortal: manually curated resource of macromolecular complexes

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ComplexPortali
CPX-3227, Core mediator complex

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q71SY5

Database of interacting proteins

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DIPi
DIP-31454N

Protein interaction database and analysis system

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IntActi
Q71SY5, 54 interactors

Molecular INTeraction database

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MINTi
Q71SY5

STRING: functional protein association networks

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STRINGi
9606.ENSP00000326767

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
Q71SY5, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1747
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...
BMRBi
Q71SY5

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q71SY5

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q71SY5

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 226Interaction with the Mediator complexAdd BLAST226
Regioni389 – 543Interaction with VP16Add BLAST155
Regioni395 – 545Interaction with CREBBP1 PublicationAdd BLAST151
Regioni564 – 653Interaction with RARA1 PublicationAdd BLAST90
Regioni640 – 707Interaction with RARA1 PublicationAdd BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi646 – 650LXXLL motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi200 – 381Pro-richAdd BLAST182
Compositional biasi565 – 735Pro-richAdd BLAST171

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mediator complex subunit 25 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
ENOG502QRN5, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000160439

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_007594_0_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q71SY5

KEGG Orthology (KO)

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KOi
K15168

Identification of Orthologs from Complete Genome Data

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OMAi
QRQNNMM

Database of Orthologous Groups

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OrthoDBi
340324at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q71SY5

TreeFam database of animal gene trees

More...
TreeFami
TF329598

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
2.40.290.30, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR021394, Med25_PTOV
IPR021406, Mediator_Med25_NR-box
IPR021397, Mediator_Med25_SD1
IPR021419, Mediator_Med25_VWA
IPR038196, PTOV_dom_sf
IPR036465, vWFA_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF11232, Med25, 1 hit
PF11244, Med25_NR-box, 1 hit
PF11235, Med25_SD1, 1 hit
PF11265, Med25_VWA, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53300, SSF53300, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q71SY5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG
60 70 80 90 100
PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG
110 120 130 140 150
IKFMGGGGES CSLIAEGLST ALQLFDDFKK MREQIGQTHR VCLLICNSPP
160 170 180 190 200
YLLPAVESTT YSGCTTENLV QQIGERGIHF SIVSPRKLPA LRLLFEKAAP
210 220 230 240 250
PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ SKQPVPLPPA
260 270 280 290 300
APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG
310 320 330 340 350
PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV
360 370 380 390 400
APGSGLAPTA QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL
410 420 430 440 450
AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM
460 470 480 490 500
QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF
510 520 530 540 550
PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITNHKQVQQQ
560 570 580 590 600
KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG
610 620 630 640 650
ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL
660 670 680 690 700
LNPPPPQTGV PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP
710 720 730 740
AQSWPAQLPP RAPLPGQMLL SGGPRGPVPQ PGLQPSVMED DILMDLI
Length:747
Mass (Da):78,171
Last modified:October 2, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6FE13FB45786402D
GO
Isoform 4 (identifier: Q71SY5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     716-747: GQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI → AAKRKREGEG...IRARPIWPDP

Show »
Length:793
Mass (Da):84,389
Checksum:i4F9597470EB0E517
GO
Isoform 5 (identifier: Q71SY5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-319: Missing.

Show »
Length:730
Mass (Da):76,512
Checksum:i70D41D4F28B8A709
GO
Isoform 6 (identifier: Q71SY5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-273: Missing.

Show »
Length:534
Mass (Da):55,456
Checksum:iD49EA118755469AC
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QZQ2M0QZQ2_HUMAN
Mediator complex subunit 25
MED25
796Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
B9TX31B9TX31_HUMAN
Mediator complex subunit 25
MED25
482Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A087WTR5A0A087WTR5_HUMAN
Mediator of RNA polymerase II trans...
MED25
180Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
B9TX33B9TX33_HUMAN
Mediator of RNA polymerase II trans...
MED25
397Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A087WYW9A0A087WYW9_HUMAN
Mediator of RNA polymerase II trans...
MED25
233Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QYR4M0QYR4_HUMAN
Mediator of RNA polymerase II trans...
MED25
253Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A087WW16A0A087WW16_HUMAN
Mediator of RNA polymerase II trans...
MED25
259Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAG15589 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS45401 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated
The sequence CAB66680 differs from that shown. Non-canonical splice intron-exon junction.Curated
The sequence CAB66680 differs from that shown. Reason: Frameshift.Curated
The sequence CAE84581 differs from that shown. Non-canonical splice intron-exon junction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti20F → S in AAM20739 (PubMed:12163014).Curated1
Sequence conflicti236P → L in CAE84581 (PubMed:14657022).Curated1
Sequence conflicti236P → L in CAB66680 (Ref. 4) Curated1
Sequence conflicti268V → I in AAM20739 (PubMed:12163014).Curated1
Sequence conflicti514L → M in AAS45401 (PubMed:14983011).Curated1
Sequence conflicti536G → D in AAS45401 (PubMed:14983011).Curated1
Sequence conflicti703S → F in AAM20739 (PubMed:12163014).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07394939Y → C in BVSYS; the mutation impairs interaction with the Mediator complex. 1 PublicationCorresponds to variant dbSNP:rs794729668EnsemblClinVar.1
Natural variantiVAR_073950140R → W Found in a patient with syndromic intellectual disability; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs781140315EnsemblClinVar.1
Natural variantiVAR_063521335A → V in CMT2B2. 1 PublicationCorresponds to variant dbSNP:rs145770066EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_04757061 – 273Missing in isoform 6. 1 PublicationAdd BLAST213
Alternative sequenceiVSP_028143303 – 319Missing in isoform 5. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_028146716 – 747GQMLL…LMDLI → AAKRKREGEGRVFREKWERA YFFVEVKSMPMCLICKQIVS VLKEYNLKRHYESKHSKSYD QYTEQTRRIRARPIWPDP in isoform 4. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF261072 mRNA Translation: AAM20739.1
AJ617479 mRNA Translation: CAE84581.1 Sequence problems.
AY533507 mRNA Translation: AAS45401.1 Sequence problems.
AL136746 mRNA Translation: CAB66680.1 Sequence problems.
EU392500 mRNA Translation: ACB88862.1
AK289460 mRNA Translation: BAF82149.1
AC006942 Genomic DNA Translation: AAD15565.1
AC018766 Genomic DNA No translation available.
CH471177 Genomic DNA Translation: EAW52546.1
BC024312 mRNA Translation: AAH24312.2
BC065297 mRNA Translation: AAH65297.1
AF283769 mRNA Translation: AAG15589.1 Different initiation.

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS33075.1 [Q71SY5-1]

NCBI Reference Sequences

More...
RefSeqi
NP_112235.2, NM_030973.3 [Q71SY5-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000312865; ENSP00000326767; ENSG00000104973 [Q71SY5-1]
ENST00000538643; ENSP00000437496; ENSG00000104973 [Q71SY5-6]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
81857

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:81857

UCSC genome browser

More...
UCSCi
uc002ppw.3, human [Q71SY5-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF261072 mRNA Translation: AAM20739.1
AJ617479 mRNA Translation: CAE84581.1 Sequence problems.
AY533507 mRNA Translation: AAS45401.1 Sequence problems.
AL136746 mRNA Translation: CAB66680.1 Sequence problems.
EU392500 mRNA Translation: ACB88862.1
AK289460 mRNA Translation: BAF82149.1
AC006942 Genomic DNA Translation: AAD15565.1
AC018766 Genomic DNA No translation available.
CH471177 Genomic DNA Translation: EAW52546.1
BC024312 mRNA Translation: AAH24312.2
BC065297 mRNA Translation: AAH65297.1
AF283769 mRNA Translation: AAG15589.1 Different initiation.
CCDSiCCDS33075.1 [Q71SY5-1]
RefSeqiNP_112235.2, NM_030973.3 [Q71SY5-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KY6NMR-A391-553[»]
2L23NMR-A391-548[»]
2L6UNMR-A391-543[»]
2XNFNMR-A394-543[»]
BMRBiQ71SY5
SMRiQ71SY5
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi123607, 72 interactors
ComplexPortaliCPX-3227, Core mediator complex
CORUMiQ71SY5
DIPiDIP-31454N
IntActiQ71SY5, 54 interactors
MINTiQ71SY5
STRINGi9606.ENSP00000326767

PTM databases

iPTMnetiQ71SY5
PhosphoSitePlusiQ71SY5

Polymorphism and mutation databases

BioMutaiMED25
DMDMi158706143

Proteomic databases

jPOSTiQ71SY5
MassIVEiQ71SY5
MaxQBiQ71SY5
PaxDbiQ71SY5
PeptideAtlasiQ71SY5
PRIDEiQ71SY5
ProteomicsDBi68625 [Q71SY5-1]
68628 [Q71SY5-4]
68629 [Q71SY5-5]
7538

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
45928, 115 antibodies

The DNASU plasmid repository

More...
DNASUi
81857

Genome annotation databases

EnsembliENST00000312865; ENSP00000326767; ENSG00000104973 [Q71SY5-1]
ENST00000538643; ENSP00000437496; ENSG00000104973 [Q71SY5-6]
GeneIDi81857
KEGGihsa:81857
UCSCiuc002ppw.3, human [Q71SY5-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
81857
DisGeNETi81857
EuPathDBiHostDB:ENSG00000104973.14

GeneCards: human genes, protein and diseases

More...
GeneCardsi
MED25
GeneReviewsiMED25
HGNCiHGNC:28845, MED25
HPAiENSG00000104973, Low tissue specificity
MalaCardsiMED25
MIMi605589, phenotype
610197, gene
616449, phenotype
neXtProtiNX_Q71SY5
OpenTargetsiENSG00000104973
Orphaneti88616, Autosomal recessive non-syndromic intellectual disability
101101, Charcot-Marie-Tooth disease type 2B2
464738, Congenital cataract-microcephaly-nevus flammeus simplex-severe intellectual disability syndrome
PharmGKBiPA134984839

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiENOG502QRN5, Eukaryota
GeneTreeiENSGT00940000160439
HOGENOMiCLU_007594_0_0_1
InParanoidiQ71SY5
KOiK15168
OMAiQRQNNMM
OrthoDBi340324at2759
PhylomeDBiQ71SY5
TreeFamiTF329598

Enzyme and pathway databases

PathwayCommonsiQ71SY5
ReactomeiR-HSA-1989781, PPARA activates gene expression
R-HSA-212436, Generic Transcription Pathway
R-HSA-381340, Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
81857, 134 hits in 876 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
MED25, human
EvolutionaryTraceiQ71SY5

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
MED25

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
81857
PharosiQ71SY5, Tbio

Protein Ontology

More...
PROi
PR:Q71SY5
RNActiQ71SY5, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000104973, Expressed in oocyte and 218 other tissues
ExpressionAtlasiQ71SY5, baseline and differential
GenevisibleiQ71SY5, HS

Family and domain databases

Gene3Di2.40.290.30, 1 hit
InterProiView protein in InterPro
IPR021394, Med25_PTOV
IPR021406, Mediator_Med25_NR-box
IPR021397, Mediator_Med25_SD1
IPR021419, Mediator_Med25_VWA
IPR038196, PTOV_dom_sf
IPR036465, vWFA_dom_sf
PfamiView protein in Pfam
PF11232, Med25, 1 hit
PF11244, Med25_NR-box, 1 hit
PF11235, Med25_SD1, 1 hit
PF11265, Med25_VWA, 1 hit
SUPFAMiSSF53300, SSF53300, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMED25_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q71SY5
Secondary accession number(s): A8K095
, B9TX30, O95783, Q6P143, Q6QMH5, Q707U4, Q8TB55, Q9H0L5, Q9HB34
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: October 7, 2020
This is version 129 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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