UniProtKB - Q71RJ2 (CCG2_RAT)
Protein
Voltage-dependent calcium channel gamma-2 subunit
Gene
Cacng2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.5 Publications
GO - Molecular functioni
- calcium channel regulator activity Source: RGD
- channel regulator activity Source: GO_Central
- ionotropic glutamate receptor binding Source: RGD
- voltage-gated calcium channel activity Source: RGD
GO - Biological processi
- eye blink reflex Source: RGD
- membrane depolarization Source: RGD
- membrane hyperpolarization Source: RGD
- nervous system process Source: RGD
- neuromuscular junction development Source: RGD
- neurotransmitter receptor internalization Source: RGD
- neurotransmitter receptor localization to postsynaptic specialization membrane Source: RGD
- neurotransmitter receptor transport, postsynaptic endosome to lysosome Source: RGD
- positive regulation of AMPA receptor activity Source: RGD
- positive regulation of protein localization to basolateral plasma membrane Source: RGD
- positive regulation of synaptic transmission, glutamatergic Source: GO_Central
- postsynaptic neurotransmitter receptor diffusion trapping Source: RGD
- protein targeting to membrane Source: RGD
- regulation of AMPA receptor activity Source: UniProtKB
- regulation of membrane potential Source: RGD
- regulation of postsynaptic membrane neurotransmitter receptor levels Source: RGD
- response to calcium ion Source: RGD
- transmission of nerve impulse Source: RGD
Keywordsi
Molecular function | Calcium channel, Ion channel, Voltage-gated channel |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-RNO-112308, Presynaptic depolarization and calcium channel opening R-RNO-399719, Trafficking of AMPA receptors R-RNO-5576892, Phase 0 - rapid depolarisation R-RNO-5576893, Phase 2 - plateau phase R-RNO-5682910, LGI-ADAM interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent calcium channel gamma-2 subunitAlternative name(s): Neuronal voltage-gated calcium channel gamma-2 subunit Stargazin1 Publication Transmembrane AMPAR regulatory protein gamma-2 Short name: TARP gamma-21 Publication |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 71095, Cacng2 |
Subcellular locationi
Other locations
- Membrane ; Multi-pass membrane protein
- synaptosome 1 Publication
Cytosol
- cytosol Source: RGD
Plasma Membrane
- AMPA glutamate receptor complex Source: UniProtKB
- integral component of postsynaptic density membrane Source: RGD
- plasma membrane Source: RGD
- postsynaptic density membrane Source: SynGO
- voltage-gated calcium channel complex Source: InterPro
Other locations
- cell surface Source: RGD
- cerebellar mossy fiber Source: RGD
- glutamatergic synapse Source: RGD
- hippocampal mossy fiber to CA3 synapse Source: RGD
- postsynaptic density Source: RGD
- Schaffer collateral - CA1 synapse Source: RGD
- somatodendritic compartment Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 104 – 124 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 134 – 154 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 182 – 202 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell junction, Membrane, Synapse, SynaptosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000408973 | 1 – 323 | Voltage-dependent calcium channel gamma-2 subunitAdd BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 48 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 253 | PhosphoserineCombined sources | 1 | |
Modified residuei | 271 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 321 | Phosphothreonine; by PKABy similarity | 1 |
Post-translational modificationi
Phosphorylation of Thr-321 by PKA impairs interaction with DLG1 and DLG4.By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q71RJ2 |
PRIDEi | Q71RJ2 |
PTM databases
GlyGeni | Q71RJ2, 1 site |
iPTMneti | Q71RJ2 |
PhosphoSitePlusi | Q71RJ2 |
SwissPalmi | Q71RJ2 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000006226, Expressed in brain and 7 other tissues |
Genevisiblei | Q71RJ2, RN |
Interactioni
Subunit structurei
The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma.
Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97 (PubMed:27756895). May interact with GOPC (By similarity). Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs).
Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8.
Interacts with GRIA1 and GRIA2 (PubMed:27756895).
Interacts with MPP2 (PubMed:27756895).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ71RJ2
With | #Exp. | IntAct |
---|---|---|
Dlg4 [P31016] | 2 | EBI-8538384,EBI-375655 |
Gria1 [P19490] | 7 | EBI-8538384,EBI-371642 |
Gria2 [P19491] | 2 | EBI-8538384,EBI-77718 |
GO - Molecular functioni
- ionotropic glutamate receptor binding Source: RGD
Protein-protein interaction databases
CORUMi | Q71RJ2 |
DIPi | DIP-41720N |
IntActi | Q71RJ2, 5 interactors |
MINTi | Q71RJ2 |
STRINGi | 10116.ENSRNOP00000008414 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSNI, Eukaryota |
GeneTreei | ENSGT01000000214567 |
HOGENOMi | CLU_053704_0_1_1 |
InParanoidi | Q71RJ2 |
OMAi | CIQKESK |
PhylomeDBi | Q71RJ2 |
TreeFami | TF327980 |
Family and domain databases
InterProi | View protein in InterPro IPR004031, PMP22/EMP/MP20/Claudin IPR005422, VDCC_g2su IPR008368, VDCC_gsu |
PANTHERi | PTHR12107:SF1, PTHR12107:SF1, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01792, VDCCGAMMA PR01602, VDCCGAMMA2 |
i Sequence
Sequence statusi: Complete.
Q71RJ2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLFDRGVQM LLTTVGAFAA FSLMTIAVGT DYWLYSRGVC KTKSVSENET
60 70 80 90 100
SKKNEEVMTH SGLWRTCCLE GNFKGLCKQI DHFPEDADYE ADTAEYFLRA
110 120 130 140 150
VRASSIFPIL SVILLFMGGL CIAASEFYKT RHNIILSAGI FFVSAGLSNI
160 170 180 190 200
IGIIVYISAN AGDPSKSDSK KNSYSYGWSF YFGALSFIIA EMVGVLAVHM
210 220 230 240 250
FIDRHKQLRA TARATDYLQA SAITRIPSYR YRYQRRSRSS SRSTEPSHSR
260 270 280 290 300
DASPVGVKGF NTLPSTEISM YTLSRDPLKA ATTPTATYNS DRDNSFLQVH
310 320
NCIQKDSKDS LHANTANRRT TPV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF361339 mRNA Translation: AAL50034.1 CH473950 Genomic DNA Translation: EDM15892.1 |
Genome annotation databases
Ensembli | ENSRNOT00000008414; ENSRNOP00000008414; ENSRNOG00000006226 |
UCSCi | RGD:71095, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF361339 mRNA Translation: AAL50034.1 CH473950 Genomic DNA Translation: EDM15892.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5KK2 | electron microscopy | 7.30 | E/F/G/H | 1-323 | [»] | |
5VOT | electron microscopy | 4.90 | E/F/G/H | 1-323 | [»] | |
5VOU | electron microscopy | 6.40 | E/F/G/H | 1-323 | [»] | |
5VOV | electron microscopy | 7.70 | E/F/G/H | 1-323 | [»] | |
6NJL | electron microscopy | 6.70 | F/H | 1-323 | [»] | |
6NJM | electron microscopy | 6.50 | F/H | 1-323 | [»] | |
6NJN | electron microscopy | 6.50 | F/H | 1-323 | [»] | |
SMRi | Q71RJ2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | Q71RJ2 |
DIPi | DIP-41720N |
IntActi | Q71RJ2, 5 interactors |
MINTi | Q71RJ2 |
STRINGi | 10116.ENSRNOP00000008414 |
PTM databases
GlyGeni | Q71RJ2, 1 site |
iPTMneti | Q71RJ2 |
PhosphoSitePlusi | Q71RJ2 |
SwissPalmi | Q71RJ2 |
Proteomic databases
PaxDbi | Q71RJ2 |
PRIDEi | Q71RJ2 |
Protocols and materials databases
ABCDi | Q71RJ2, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000008414; ENSRNOP00000008414; ENSRNOG00000006226 |
UCSCi | RGD:71095, rat |
Organism-specific databases
RGDi | 71095, Cacng2 |
Phylogenomic databases
eggNOGi | ENOG502QSNI, Eukaryota |
GeneTreei | ENSGT01000000214567 |
HOGENOMi | CLU_053704_0_1_1 |
InParanoidi | Q71RJ2 |
OMAi | CIQKESK |
PhylomeDBi | Q71RJ2 |
TreeFami | TF327980 |
Enzyme and pathway databases
Reactomei | R-RNO-112308, Presynaptic depolarization and calcium channel opening R-RNO-399719, Trafficking of AMPA receptors R-RNO-5576892, Phase 0 - rapid depolarisation R-RNO-5576893, Phase 2 - plateau phase R-RNO-5682910, LGI-ADAM interactions |
Miscellaneous databases
PROi | PR:Q71RJ2 |
Gene expression databases
Bgeei | ENSRNOG00000006226, Expressed in brain and 7 other tissues |
Genevisiblei | Q71RJ2, RN |
Family and domain databases
InterProi | View protein in InterPro IPR004031, PMP22/EMP/MP20/Claudin IPR005422, VDCC_g2su IPR008368, VDCC_gsu |
PANTHERi | PTHR12107:SF1, PTHR12107:SF1, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01792, VDCCGAMMA PR01602, VDCCGAMMA2 |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCG2_RAT | |
Accessioni | Q71RJ2Primary (citable) accession number: Q71RJ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 31, 2011 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families