UniProtKB - Q71RI9 (KAT3_MOUSE)
Protein
Kynurenine--oxoglutarate transaminase 3
Gene
Kyat3
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others (PubMed:19029248). May catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (PubMed:19029248). Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro) (PubMed:19029248).1 Publication
Catalytic activityi
- EC:2.6.1.71 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:2.6.1.631 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:2.6.1.631 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.4.1.131 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
pyridoxal 5'-phosphate1 Publication
Activity regulationi
Kynurenine transamination is competitively inhibited by cysteine, glutamine, histidine, methionine, leucine, or phenylalanine.1 Publication
Kineticsi
- KM=0.7 mM for glutamine1 Publication
- KM=0.7 mM for histidine1 Publication
- KM=0.7 mM for cysteine1 Publication
- KM=0.9 mM for methionine1 Publication
- KM=1.1 mM for phenylalanine1 Publication
- KM=1.4 mM for asparagine1 Publication
- KM=1.5 mM for kynurenine1 Publication
- KM=7.1 mM for tryptophan1 Publication
- KM=3.0 mM for serine1 Publication
- KM=0.4 mM for glyoxylate1 Publication
- KM=0.6 mM for phenylpyruvate1 Publication
pH dependencei
Optimum pH is 9.1 Publication
Temperature dependencei
Optimum temperature is 60 degrees Celsius.1 Publication
: L-kynurenine degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes kynurenate from L-kynurenine.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Kynurenine--oxoglutarate transaminase 3 (Kyat3), Kynurenine--oxoglutarate transaminase 1 (Kyat1)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes kynurenate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 72 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 219 | SubstrateBy similarity | 1 | |
Binding sitei | 430 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- cysteine-S-conjugate beta-lyase activity Source: UniProtKB-EC
- kynurenine-glyoxylate transaminase activity Source: UniProtKB
- kynurenine-oxoglutarate transaminase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- biosynthetic process Source: InterPro
- cellular amino acid metabolic process Source: UniProtKB
- kynurenine metabolic process Source: UniProtKB
Keywordsi
Molecular function | Aminotransferase, Lyase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 2.6.1.7, 3474 |
UniPathwayi | UPA00334;UER00726 |
Names & Taxonomyi
Protein namesi | Recommended name: Kynurenine--oxoglutarate transaminase 31 Publication (EC:2.6.1.71 Publication)Alternative name(s): |
Gene namesi | Name:Kyat3 Synonyms:Ccbl2, Kat3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2677849, Kyat3 |
Subcellular locationi
Mitochondrion
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287705 | 1 – 455 | Kynurenine--oxoglutarate transaminase 3Add BLAST | 455 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 117 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 117 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 281 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | Q71RI9 |
PaxDbi | Q71RI9 |
PeptideAtlasi | Q71RI9 |
PRIDEi | Q71RI9 |
PTM databases
iPTMneti | Q71RI9 |
PhosphoSitePlusi | Q71RI9 |
SwissPalmi | Q71RI9 |
Expressioni
Tissue specificityi
Widely expressed, with higher expression levels in liver, kidney, heart and neuroendocrine tissues.1 Publication
Developmental stagei
Expressed from postnatal day (PND) 7 and peaks in adult.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040213, Expressed in liver and 257 other tissues |
Genevisiblei | Q71RI9, MM |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 230916, 1 interactor |
IntActi | Q71RI9, 3 interactors |
MINTi | Q71RI9 |
STRINGi | 10090.ENSMUSP00000041675 |
Miscellaneous databases
RNActi | Q71RI9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q71RI9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0257, Eukaryota |
GeneTreei | ENSGT00940000155827 |
HOGENOMi | CLU_017584_4_0_1 |
InParanoidi | Q71RI9 |
OMAi | SVAMTGW |
OrthoDBi | 683031at2759 |
TreeFami | TF352342 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR034612, KAT_III IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR43807:SF6, PTHR43807:SF6, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q71RI9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLLAQRRLIS LGCRSKPIKT IYSSSKVLGL CTSAKMALKF KNAKRIEGLD
60 70 80 90 100
SNVWVEFTKL AADPSVVNLG QGFPDISPPS YVKEELSKAA FIDNMNQYTR
110 120 130 140 150
GFGHPALVKA LSCLYGKIYQ RQIDPNEEIL VAVGAYGSLF NSIQGLVDPG
160 170 180 190 200
DEVIIMVPFY DCYEPMVRMA GAVPVFIPLR SKPTDGMKWT SSDWTFDPRE
210 220 230 240 250
LESKFSSKTK AIILNTPHNP LGKVYTRQEL QVIADLCVKH DTLCISDEVY
260 270 280 290 300
EWLVYTGHTH VKIATLPGMW ERTITIGSAG KTFSVTGWKL GWSIGPAHLI
310 320 330 340 350
KHLQTVQQNS FYTCATPLQA ALAEAFWIDI KRMDDPECYF NSLPKELEVK
360 370 380 390 400
RDRMVRLLNS VGLKPIVPDG GYFIIADVSS LGADLSDMNS DEPYDYKFVK
410 420 430 440 450
WMTKHKKLTA IPVSAFCDSK SKPHFEKLVR FCFIKKDSTL DAAEEIFRAW
NSQKS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JGE1 | A0A0G2JGE1_MOUSE | Cysteine-S-conjugate beta-lyase 2 | Kyat3 | 194 | Annotation score: | ||
D6RJ70 | D6RJ70_MOUSE | Kynurenine--oxoglutarate transamina... | Kyat3 | 71 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025605 | 1 – 35 | Missing in isoform 2. 2 PublicationsAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF363737 mRNA Translation: AAQ15190.1 AK049569 mRNA Translation: BAC33817.1 AK145623 mRNA Translation: BAE26546.1 BC131942 mRNA Translation: AAI31943.1 BC132615 mRNA Translation: AAI32616.1 |
CCDSi | CCDS17881.1 [Q71RI9-2] CCDS80040.1 [Q71RI9-1] |
RefSeqi | NP_001280489.1, NM_001293560.1 [Q71RI9-1] NP_776124.1, NM_173763.4 [Q71RI9-2] XP_006501494.1, XM_006501431.3 [Q71RI9-2] XP_011238416.1, XM_011240114.2 [Q71RI9-2] |
Genome annotation databases
Ensembli | ENSMUST00000044392; ENSMUSP00000041675; ENSMUSG00000040213 [Q71RI9-2] ENSMUST00000106218; ENSMUSP00000101825; ENSMUSG00000040213 [Q71RI9-1] |
GeneIDi | 229905 |
KEGGi | mmu:229905 |
UCSCi | uc008roz.2, mouse [Q71RI9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF363737 mRNA Translation: AAQ15190.1 AK049569 mRNA Translation: BAC33817.1 AK145623 mRNA Translation: BAE26546.1 BC131942 mRNA Translation: AAI31943.1 BC132615 mRNA Translation: AAI32616.1 |
CCDSi | CCDS17881.1 [Q71RI9-2] CCDS80040.1 [Q71RI9-1] |
RefSeqi | NP_001280489.1, NM_001293560.1 [Q71RI9-1] NP_776124.1, NM_173763.4 [Q71RI9-2] XP_006501494.1, XM_006501431.3 [Q71RI9-2] XP_011238416.1, XM_011240114.2 [Q71RI9-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZJG | X-ray | 3.00 | A/B | 42-451 | [»] | |
3E2F | X-ray | 2.59 | A/B | 42-451 | [»] | |
3E2Y | X-ray | 2.26 | A/B | 42-451 | [»] | |
3E2Z | X-ray | 2.81 | A/B | 42-451 | [»] | |
5VEP | X-ray | 2.59 | A/B | 42-451 | [»] | |
5VEQ | X-ray | 2.26 | A/B | 42-451 | [»] | |
5VER | X-ray | 2.81 | A/B | 42-451 | [»] | |
SMRi | Q71RI9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 230916, 1 interactor |
IntActi | Q71RI9, 3 interactors |
MINTi | Q71RI9 |
STRINGi | 10090.ENSMUSP00000041675 |
PTM databases
iPTMneti | Q71RI9 |
PhosphoSitePlusi | Q71RI9 |
SwissPalmi | Q71RI9 |
Proteomic databases
jPOSTi | Q71RI9 |
PaxDbi | Q71RI9 |
PeptideAtlasi | Q71RI9 |
PRIDEi | Q71RI9 |
Protocols and materials databases
Antibodypediai | 19822, 84 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044392; ENSMUSP00000041675; ENSMUSG00000040213 [Q71RI9-2] ENSMUST00000106218; ENSMUSP00000101825; ENSMUSG00000040213 [Q71RI9-1] |
GeneIDi | 229905 |
KEGGi | mmu:229905 |
UCSCi | uc008roz.2, mouse [Q71RI9-1] |
Organism-specific databases
CTDi | 56267 |
MGIi | MGI:2677849, Kyat3 |
Phylogenomic databases
eggNOGi | KOG0257, Eukaryota |
GeneTreei | ENSGT00940000155827 |
HOGENOMi | CLU_017584_4_0_1 |
InParanoidi | Q71RI9 |
OMAi | SVAMTGW |
OrthoDBi | 683031at2759 |
TreeFami | TF352342 |
Enzyme and pathway databases
UniPathwayi | UPA00334;UER00726 |
BRENDAi | 2.6.1.7, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 229905, 0 hits in 17 CRISPR screens |
ChiTaRSi | Kyat3, mouse |
PROi | PR:Q71RI9 |
RNActi | Q71RI9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040213, Expressed in liver and 257 other tissues |
Genevisiblei | Q71RI9, MM |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR034612, KAT_III IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR43807:SF6, PTHR43807:SF6, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAT3_MOUSE | |
Accessioni | Q71RI9Primary (citable) accession number: Q71RI9 Secondary accession number(s): Q8BJ84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families