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UniProtKB - Q71KT5 (ERG24_MOUSE)
Protein
Delta(14)-sterol reductase TM7SF2
Gene
Tm7sf2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis.
1 PublicationCatalytic activityi
- 4,4-dimethyl-5α-cholesta-8,24-dien-3β-ol + NADP+ = 4,4-dimethyl-5α-cholesta-8,14,24-trien-3β-ol + H+ + NADPHBy similarityEC:1.3.1.70By similarity
- 4,4-dimethyl-8,14-cholestadien-3β-ol + H+ + NADPH = 4,4-dimethyl-5α-cholest-8-en-3β-ol + NADP+By similarity
: cholesterol biosynthesis Pathwayi
This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.View all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 311 | NADPBy similarity | 1 | |
Binding sitei | 315 | NADPBy similarity | 1 | |
Binding sitei | 338 | NADP; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 343 | NADPBy similarity | 1 | |
Binding sitei | 390 | NADPBy similarity | 1 | |
Binding sitei | 405 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 350 – 351 | NADPBy similarity | 2 | |
Nucleotide bindingi | 394 – 398 | NADPBy similarity | 5 |
GO - Molecular functioni
- delta14-sterol reductase activity Source: UniProtKB
- NADP binding Source: UniProtKB
- oxidoreductase activity, acting on the CH-CH group of donors Source: GO_Central
GO - Biological processi
- cholesterol biosynthetic process Source: UniProtKB
- sterol biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.3.1.70, 3474 |
Reactomei | R-MMU-191273, Cholesterol biosynthesis |
UniPathwayi | UPA00063 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta(14)-sterol reductase TM7SF2 (EC:1.3.1.70By similarity)Short name: Delta-14-SR Alternative name(s): 3-beta-hydroxysterol Delta (14)-reductaseBy similarity C-14 sterol reductase Short name: C14SR Sterol C14-reductase Transmembrane 7 superfamily member 2 |
Gene namesi | Name:Tm7sf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920416, Tm7sf2 |
VEuPathDBi | HostDB:ENSMUSG00000024799 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
- Microsome membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: MGI
- integral component of endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nuclear inner membrane Source: GO_Central
Other locations
- intracellular membrane-bounded organelle Source: MGI
- organelle membrane Source: UniProtKB
- receptor complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 13 – 35 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 62 – 81 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 102 – 124 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 129 – 148 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 255 – 277 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 287 – 304 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 355 – 377 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Disruption phenotypei
Mice develop normally, appear healthy and are fertile.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000331121 | 1 – 418 | Delta(14)-sterol reductase TM7SF2Add BLAST | 418 |
Proteomic databases
jPOSTi | Q71KT5 |
MaxQBi | Q71KT5 |
PaxDbi | Q71KT5 |
PeptideAtlasi | Q71KT5 |
PRIDEi | Q71KT5 |
ProteomicsDBi | 275769 |
PTM databases
iPTMneti | Q71KT5 |
PhosphoSitePlusi | Q71KT5 |
Expressioni
Tissue specificityi
Strongly expressed in liver, weaker in ovary, testis, kidney and brain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000024799, Expressed in liver and 291 other tissues |
ExpressionAtlasi | Q71KT5, baseline and differential |
Genevisiblei | Q71KT5, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000025713 |
Miscellaneous databases
RNActi | Q71KT5, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ERG4/ERG24 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1435, Eukaryota |
GeneTreei | ENSGT00390000000417 |
HOGENOMi | CLU_015631_0_1_1 |
InParanoidi | Q71KT5 |
OMAi | MSWAYCL |
OrthoDBi | 532774at2759 |
PhylomeDBi | Q71KT5 |
TreeFami | TF101179 |
Family and domain databases
InterProi | View protein in InterPro IPR001171, Ergosterol_biosynth_ERG4_ERG24 IPR018083, Sterol_reductase_CS |
Pfami | View protein in Pfam PF01222, ERG4_ERG24, 1 hit |
PROSITEi | View protein in PROSITE PS01017, STEROL_REDUCT_1, 1 hit PS01018, STEROL_REDUCT_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
Q71KT5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSREASQAP LEFGGPLGVA ALLILLPATM FHLLLAARSG PARLLALPAY
60 70 80 90 100
LPGLEELWSP WALLLLFIWL GLQVALYLLP ARKVAEGLEL KDKSRLRYPI
110 120 130 140 150
NGFQALVLTA LLMGLGVSVG LPLGALPGML LPLAFATTLT SFIFSLLLYA
160 170 180 190 200
KALVAPASAL APGGNSGNSM YDFFLGRELN PRLGSFDFKY FCELRPGLIG
210 220 230 240 250
WVFINLALLM QEAELRGSPS LAMWLVNGFQ LLYVGDALWY EESVLTTMDI
260 270 280 290 300
IHDGFGFMLV FGDLAWVPFT YSLQAQFLLY HPQPLGLPMA LLICLLKVIG
310 320 330 340 350
YYIFRGANSQ KNTFRKNPSD PSVAGLETIP TATGRQLLVS GWWGMVRHPN
360 370 380 390 400
YLGDLIMALA WSLPCGLSHL LPYFYVLYFT ALLVHREARD EQQCLQKYGR
410
AWQEYCKRVP YRIIPYVY
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CX74 | E0CX74_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 391 | Annotation score: | ||
E0CX92 | E0CX92_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 124 | Annotation score: | ||
E9Q4M8 | E9Q4M8_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 399 | Annotation score: | ||
E0CZ93 | E0CZ93_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 229 | Annotation score: | ||
A0A494BBJ1 | A0A494BBJ1_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 91 | Annotation score: | ||
Q3V3I6 | Q3V3I6_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 110 | Annotation score: | ||
E0CYD6 | E0CYD6_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 197 | Annotation score: | ||
E0CX82 | E0CX82_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 61 | Annotation score: | ||
E0CZB2 | E0CZB2_MOUSE | Delta(14)-sterol reductase TM7SF2 | Tm7sf2 | 89 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 298 | V → A in AAQ05836 (Ref. 1) Curated | 1 | |
Sequence conflicti | 298 | V → A in BAE33285 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF480070 mRNA Translation: AAQ05836.1 EU672836 Genomic DNA Translation: ACF94776.1 AK155475 mRNA Translation: BAE33285.1 CH466612 Genomic DNA Translation: EDL33202.1 |
CCDSi | CCDS29490.2 |
RefSeqi | NP_082730.2, NM_028454.2 |
Genome annotation databases
Ensembli | ENSMUST00000025713; ENSMUSP00000025713; ENSMUSG00000024799 |
GeneIDi | 73166 |
KEGGi | mmu:73166 |
UCSCi | uc008ggw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF480070 mRNA Translation: AAQ05836.1 EU672836 Genomic DNA Translation: ACF94776.1 AK155475 mRNA Translation: BAE33285.1 CH466612 Genomic DNA Translation: EDL33202.1 |
CCDSi | CCDS29490.2 |
RefSeqi | NP_082730.2, NM_028454.2 |
3D structure databases
SMRi | Q71KT5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000025713 |
PTM databases
iPTMneti | Q71KT5 |
PhosphoSitePlusi | Q71KT5 |
Proteomic databases
jPOSTi | Q71KT5 |
MaxQBi | Q71KT5 |
PaxDbi | Q71KT5 |
PeptideAtlasi | Q71KT5 |
PRIDEi | Q71KT5 |
ProteomicsDBi | 275769 |
Protocols and materials databases
Antibodypediai | 29654, 121 antibodies from 23 providers |
DNASUi | 73166 |
Genome annotation databases
Ensembli | ENSMUST00000025713; ENSMUSP00000025713; ENSMUSG00000024799 |
GeneIDi | 73166 |
KEGGi | mmu:73166 |
UCSCi | uc008ggw.1, mouse |
Organism-specific databases
CTDi | 7108 |
MGIi | MGI:1920416, Tm7sf2 |
VEuPathDBi | HostDB:ENSMUSG00000024799 |
Phylogenomic databases
eggNOGi | KOG1435, Eukaryota |
GeneTreei | ENSGT00390000000417 |
HOGENOMi | CLU_015631_0_1_1 |
InParanoidi | Q71KT5 |
OMAi | MSWAYCL |
OrthoDBi | 532774at2759 |
PhylomeDBi | Q71KT5 |
TreeFami | TF101179 |
Enzyme and pathway databases
UniPathwayi | UPA00063 |
BRENDAi | 1.3.1.70, 3474 |
Reactomei | R-MMU-191273, Cholesterol biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 73166, 3 hits in 67 CRISPR screens |
ChiTaRSi | Tm7sf2, mouse |
PROi | PR:Q71KT5 |
RNActi | Q71KT5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024799, Expressed in liver and 291 other tissues |
ExpressionAtlasi | Q71KT5, baseline and differential |
Genevisiblei | Q71KT5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001171, Ergosterol_biosynth_ERG4_ERG24 IPR018083, Sterol_reductase_CS |
Pfami | View protein in Pfam PF01222, ERG4_ERG24, 1 hit |
PROSITEi | View protein in PROSITE PS01017, STEROL_REDUCT_1, 1 hit PS01018, STEROL_REDUCT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ERG24_MOUSE | |
Accessioni | Q71KT5Primary (citable) accession number: Q71KT5 Secondary accession number(s): B5LBK0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families