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Protein

Centromere protein U

Gene

CENPU

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.3 Publications

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processHost-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-68877 Mitotic Prometaphase
SIGNORiQ71F23

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein U
Short name:
CENP-U
Alternative name(s):
Centromere protein of 50 kDa
Short name:
CENP-50
Interphase centromere complex protein 24
KSHV latent nuclear antigen-interacting protein 1
MLF1-interacting protein
Polo-box-interacting protein 1
Gene namesi
Name:CENPU
Synonyms:ICEN24, KLIP1, MLF1IP, PBIP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000151725.11
HGNCiHGNC:21348 CENPU
MIMi611511 gene
neXtProtiNX_Q71F23

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77S → A: Insensitive to PLK1-induced degradation. 1 Publication1
Mutagenesisi78T → A: Insensitive to PLK1-induced degradation. 1 Publication1
Mutagenesisi78T → D: Failed to enhance the PLK1-dependent degradation. 1 Publication1
Mutagenesisi78T → E: Failed to enhance the PLK1-dependent degradation. 1 Publication1

Organism-specific databases

DisGeNETi79682
OpenTargetsiENSG00000151725
PharmGKBiPA134893791

Polymorphism and mutation databases

BioMutaiMLF1IP
DMDMi74712714

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002476721 – 418Centromere protein UAdd BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78Phosphothreonine; by PLK11 Publication1
Modified residuei98PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei110PhosphothreonineCombined sources1
Modified residuei111PhosphoserineCombined sources1
Modified residuei116PhosphoserineBy similarity1
Modified residuei120PhosphoserineBy similarity1
Modified residuei136PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei190PhosphoserineBy similarity1
Modified residuei194PhosphoserineCombined sources1
Modified residuei232PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ71F23
MaxQBiQ71F23
PaxDbiQ71F23
PeptideAtlasiQ71F23
PRIDEiQ71F23
ProteomicsDBi68598
68599 [Q71F23-2]

PTM databases

iPTMnetiQ71F23
PhosphoSitePlusiQ71F23

Expressioni

Tissue specificityi

Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.3 Publications

Gene expression databases

BgeeiENSG00000151725 Expressed in 197 organ(s), highest expression level in sperm
CleanExiHS_MLF1IP
ExpressionAtlasiQ71F23 baseline and differential
GenevisibleiQ71F23 HS

Organism-specific databases

HPAiHPA022048

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with MLF1. Interacts with PLK1.4 Publications
(Microbial infection) Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA).1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi122805, 67 interactors
CORUMiQ71F23
DIPiDIP-48539N
IntActiQ71F23, 45 interactors
MINTiQ71F23
STRINGi9606.ENSP00000281453

Structurei

3D structure databases

ProteinModelPortaliQ71F23
SMRiQ71F23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili297 – 356Sequence analysisAdd BLAST60
Coiled coili397 – 417Sequence analysisAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi6 – 23Nuclear localization signalSequence analysisAdd BLAST18
Motifi303 – 320Nuclear localization signalSequence analysisAdd BLAST18

Sequence similaritiesi

Belongs to the CENP-U/AME1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J00R Eukaryota
ENOG410Z48V LUCA
GeneTreeiENSGT00390000015511
HOGENOMiHOG000236255
HOVERGENiHBG081090
InParanoidiQ71F23
KOiK11513
OMAiKQLHQDY
OrthoDBiEOG091G09Q1
PhylomeDBiQ71F23
TreeFamiTF330780

Family and domain databases

InterProiView protein in InterPro
IPR025214 CENP-U
PfamiView protein in Pfam
PF13097 CENP-U, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q71F23-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPRGRRRPR PHRSEGARRS KNTLERTHSM KDKAGQKCKP IDVFDFPDNS
60 70 80 90 100
DVSSIGRLGE NEKDEETYET FDPPLHSTAI YADEEEFSKH CGLSLSSTPP
110 120 130 140 150
GKEAKRSSDT SGNEASEIES VKISAKKPGR KLRPISDDSE SIEESDTRRK
160 170 180 190 200
VKSAEKISTQ RHEVIRTTAS SELSEKPAES VTSKKTGPLS AQPSVEKENL
210 220 230 240 250
AIESQSKTQK KGKISHDKRK KSRSKAIGSD TSDIVHIWCP EGMKTSDIKE
260 270 280 290 300
LNIVLPEFEK THLEHQQRIE SKVCKAAIAT FYVNVKEQFI KMLKESQMLT
310 320 330 340 350
NLKRKNAKMI SDIEKKRQRM IEVQDELLRL EPQLKQLQTK YDELKERKSS
360 370 380 390 400
LRNAAYFLSN LKQLYQDYSD VQAQEPNVKE TYDSSSLPAL LFKARTLLGA
410
ESHLRNINHQ LEKLLDQG
Length:418
Mass (Da):47,522
Last modified:July 5, 2004 - v1
Checksum:iA99BC012EF7188F9
GO
Isoform 2 (identifier: Q71F23-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-242: Missing.

Show »
Length:176
Mass (Da):20,710
Checksum:iC5731EE5F7ADCE1D
GO
Isoform 3 (identifier: Q71F23-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     331-337: EPQLKQL → WTGAGLW
     338-418: Missing.

Show »
Length:337
Mass (Da):38,102
Checksum:i24A4BBC85B3E98E1
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6R9S4D6R9S4_HUMAN
Centromere protein U
CENPU
177Annotation score:
Q09GN1Q09GN1_HUMAN
Centromere protein U
CENPU MLF1IP
320Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163E → G in AAI07745 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04869216G → R. Corresponds to variant dbSNP:rs902174Ensembl.1
Natural variantiVAR_02714416G → S. Corresponds to variant dbSNP:rs902174Ensembl.1
Natural variantiVAR_027145157I → T. Corresponds to variant dbSNP:rs6552804Ensembl.1
Natural variantiVAR_027146214I → M. Corresponds to variant dbSNP:rs4616798Ensembl.1
Natural variantiVAR_048693279A → T. Corresponds to variant dbSNP:rs34007339Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0200301 – 242Missing in isoform 2. 3 PublicationsAdd BLAST242
Alternative sequenceiVSP_053526331 – 337EPQLKQL → WTGAGLW in isoform 3. 1 Publication7
Alternative sequenceiVSP_053527338 – 418Missing in isoform 3. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469667 mRNA Translation: AAQ05290.1
AF516710 mRNA Translation: AAQ08228.1
AK027121 mRNA Translation: BAB15665.1
CR457376 mRNA Translation: CAG33657.1
DQ907910 mRNA Translation: ABI49142.1
AC079257 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX04666.1
BC107744 mRNA Translation: AAI07745.1
BC031520 mRNA Translation: AAH31520.1
BC131556 mRNA Translation: AAI31557.1
CCDSiCCDS3838.1 [Q71F23-1]
RefSeqiNP_078905.2, NM_024629.3 [Q71F23-1]
UniGeneiHs.575032

Genome annotation databases

EnsembliENST00000281453; ENSP00000281453; ENSG00000151725 [Q71F23-1]
GeneIDi79682
KEGGihsa:79682
UCSCiuc003iwq.4 human [Q71F23-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469667 mRNA Translation: AAQ05290.1
AF516710 mRNA Translation: AAQ08228.1
AK027121 mRNA Translation: BAB15665.1
CR457376 mRNA Translation: CAG33657.1
DQ907910 mRNA Translation: ABI49142.1
AC079257 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX04666.1
BC107744 mRNA Translation: AAI07745.1
BC031520 mRNA Translation: AAH31520.1
BC131556 mRNA Translation: AAI31557.1
CCDSiCCDS3838.1 [Q71F23-1]
RefSeqiNP_078905.2, NM_024629.3 [Q71F23-1]
UniGeneiHs.575032

3D structure databases

ProteinModelPortaliQ71F23
SMRiQ71F23
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122805, 67 interactors
CORUMiQ71F23
DIPiDIP-48539N
IntActiQ71F23, 45 interactors
MINTiQ71F23
STRINGi9606.ENSP00000281453

PTM databases

iPTMnetiQ71F23
PhosphoSitePlusiQ71F23

Polymorphism and mutation databases

BioMutaiMLF1IP
DMDMi74712714

Proteomic databases

EPDiQ71F23
MaxQBiQ71F23
PaxDbiQ71F23
PeptideAtlasiQ71F23
PRIDEiQ71F23
ProteomicsDBi68598
68599 [Q71F23-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281453; ENSP00000281453; ENSG00000151725 [Q71F23-1]
GeneIDi79682
KEGGihsa:79682
UCSCiuc003iwq.4 human [Q71F23-1]

Organism-specific databases

CTDi79682
DisGeNETi79682
EuPathDBiHostDB:ENSG00000151725.11
GeneCardsiCENPU
HGNCiHGNC:21348 CENPU
HPAiHPA022048
MIMi611511 gene
neXtProtiNX_Q71F23
OpenTargetsiENSG00000151725
PharmGKBiPA134893791
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J00R Eukaryota
ENOG410Z48V LUCA
GeneTreeiENSGT00390000015511
HOGENOMiHOG000236255
HOVERGENiHBG081090
InParanoidiQ71F23
KOiK11513
OMAiKQLHQDY
OrthoDBiEOG091G09Q1
PhylomeDBiQ71F23
TreeFamiTF330780

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-68877 Mitotic Prometaphase
SIGNORiQ71F23

Miscellaneous databases

ChiTaRSiCENPU human
GeneWikiiMLF1IP
GenomeRNAii79682
PROiPR:Q71F23
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151725 Expressed in 197 organ(s), highest expression level in sperm
CleanExiHS_MLF1IP
ExpressionAtlasiQ71F23 baseline and differential
GenevisibleiQ71F23 HS

Family and domain databases

InterProiView protein in InterPro
IPR025214 CENP-U
PfamiView protein in Pfam
PF13097 CENP-U, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCENPU_HUMAN
AccessioniPrimary (citable) accession number: Q71F23
Secondary accession number(s): A2RRD9
, Q09GN2, Q32Q71, Q9H5G1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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