Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Manganese peroxidase 2

Gene

mnp2

Organism
Phlebia radiata (White-rot fungus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of Mn2+ to Mn3+. The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds.1 Publication

Catalytic activityi

2 Mn2+ + 2 H+ + H2O2 = 2 Mn3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59ManganeseBy similarity1
Metal bindingi63ManganeseBy similarity1
Sitei66Transition state stabilizerPROSITE-ProRule annotation1
Active sitei70Proton acceptorPROSITE-ProRule annotation1
Metal bindingi71Calcium 1PROSITE-ProRule annotation1
Metal bindingi86Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi88Calcium 1PROSITE-ProRule annotation1
Metal bindingi90Calcium 1PROSITE-ProRule annotation1
Metal bindingi197Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi198Calcium 2PROSITE-ProRule annotation1
Metal bindingi203ManganeseBy similarity1
Metal bindingi215Calcium 2PROSITE-ProRule annotation1
Metal bindingi217Calcium 2PROSITE-ProRule annotation1
Metal bindingi222Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide, Lignin degradation
LigandCalcium, Heme, Iron, Manganese, Metal-binding

Protein family/group databases

CAZyiAA2 Auxiliary Activities 2
PeroxiBasei2296 PrMnP02

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese peroxidase 2 (EC:1.11.1.13)
Short name:
MnP2
Alternative name(s):
Manganese peroxidase isozyme 2
Gene namesi
Name:mnp2
OrganismiPhlebia radiata (White-rot fungus)
Taxonomic identifieri5308 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesMeruliaceaePhlebia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_500007141724 – 390Manganese peroxidase 2Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 39PROSITE-ProRule annotation
Disulfide bondi38 ↔ 313PROSITE-ProRule annotation
Disulfide bondi57 ↔ 141PROSITE-ProRule annotation
Glycosylationi155N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi277 ↔ 343PROSITE-ProRule annotation
Disulfide bondi365 ↔ 372PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ70LM3

Expressioni

Inductioni

By a combination of high manganese and malonate levels.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ70LM3
SMRiQ70LM3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20205

Family and domain databases

CDDicd00692 ligninase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR001621 Ligninase
IPR024589 Ligninase_C
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PF11895 Peroxidase_ext, 1 hit
PRINTSiPR00462 LIGNINASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q70LM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFNFAAILA FVSLAAVTSA APSKTTCSNG VVVPDAVCCD FVPLASALQS
60 70 80 90 100
EVLMGDCGED AHELVRLIFH DAIAISQSMG PSAGGGADGS MLIFPTVEPA
110 120 130 140 150
FFPNLGIADS VNNLIPFLSQ FPTISAGDLV QFAGAVAISN CPGAPQLEFL
160 170 180 190 200
AGRPNATAPA IDGLIPEPQD DVTKILARFK DAGNFSPAEV VALLASHSIA
210 220 230 240 250
RADHVDPTLD AAPFDSTPFD FDTQIFLEVL LKGVGFPGLA NNTGEVSSPL
260 270 280 290 300
PVTDGTDVGE LRLQSDFALA RDERTACAWQ SFVNEQEAMA TAFKNAVKKL
310 320 330 340 350
AVLGHNRNDL VDCSAVVPVP KPATGTPATF PASTGPQDLE LTCTTEPFPT
360 370 380 390
LSTAPGAQQT LIPHCSDGTM TCNSVQFDGP ATNFGGADDS
Length:390
Mass (Da):40,326
Last modified:July 5, 2004 - v1
Checksum:i270D7F2C36A99E50
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131Q → H in CAC85963 (PubMed:15809005).Curated1
Sequence conflicti225I → V in CAC85963 (PubMed:15809005).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ315701 mRNA Translation: CAC85963.1
AJ566199 Genomic DNA Translation: CAD92854.1

Genome annotation databases

KEGGiag:CAC85963

Similar proteinsi

Entry informationi

Entry nameiPEM2_PHLRA
AccessioniPrimary (citable) accession number: Q70LM3
Secondary accession number(s): Q96TS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health