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UniProtKB - Q70KD0 (DOIS_MICEC)
Protein
2-deoxy-scyllo-inosose synthase
Gene
gtmA
Organism
Micromonospora echinospora (Micromonospora purpurea)
Status
Functioni
Catalyzes the intramolecular carbocycle formation from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose (DOI).
1 PublicationCatalytic activityi
- EC:4.2.3.124
Cofactori
Protein has several cofactor binding sites:Temperature dependencei
Optimum temperature is 42 degrees Celsius.
: 2-deoxystreptamine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 2-deoxystreptamine from D-glucose 6-phosphate. This subpathway is part of the pathway 2-deoxystreptamine biosynthesis, which is itself part of Metabolic intermediate biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-deoxystreptamine from D-glucose 6-phosphate, the pathway 2-deoxystreptamine biosynthesis and in Metabolic intermediate biosynthesis.
Pathwayi: gentamicin biosynthesis
This protein is involved in the pathway gentamicin biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway gentamicin biosynthesis and in Antibiotic biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | NADBy similarity | 1 | |
Active sitei | 140 | By similarity | 1 | |
Metal bindingi | 182 | CobaltBy similarity | 1 | |
Active sitei | 242 | By similarity | 1 | |
Metal bindingi | 245 | CobaltBy similarity | 1 | |
Metal bindingi | 261 | CobaltBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 71 – 74 | NADBy similarity | 4 | |
Nucleotide bindingi | 103 – 107 | NADBy similarity | 5 | |
Nucleotide bindingi | 127 – 128 | NADBy similarity | 2 | |
Nucleotide bindingi | 138 – 140 | NADBy similarity | 3 | |
Nucleotide bindingi | 149 – 150 | NADBy similarity | 2 |
GO - Molecular functioni
- carbon-oxygen lyase activity, acting on phosphates Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB-KW
- aromatic amino acid family biosynthetic process Source: InterPro
Keywordsi
Molecular function | Lyase |
Biological process | Antibiotic biosynthesis |
Ligand | Cobalt, Metal-binding, NAD |
Enzyme and pathway databases
UniPathwayi | UPA00907;UER00921 UPA00967 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-deoxy-scyllo-inosose synthase (EC:4.2.3.124)Short name: DOI synthase Short name: DOIS |
Gene namesi | Name:gtmA Synonyms:genC, gntB |
Organismi | Micromonospora echinospora (Micromonospora purpurea) |
Taxonomic identifieri | 1877 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Micromonosporales › Micromonosporaceae › Micromonospora |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234035 | 1 – 397 | 2-deoxy-scyllo-inosose synthaseAdd BLAST | 397 |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR030963, DHQ_synth_fam IPR030960, DHQS/DOIS |
Pfami | View protein in Pfam PF01761, DHQ_synthase, 1 hit |
PIRSFi | PIRSF001455, DHQ_synth, 1 hit |
i Sequence
Sequence statusi: Complete.
Q70KD0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEVEIRLGSV RYPFRLGTDC LGAIVEDLVA MSASRLLIVC DSNTGPLFGA
60 70 80 90 100
ELVERLSPRV PANLLIHRAG EPYKDLQAVG TLADSALQLG ADRASVVVAV
110 120 130 140 150
GGGVIGNIAG LMAALLFRGI RLVHIPTSLI AMSDSVLSLK QAVNACVGKN
160 170 180 190 200
LMGTFYAPES VLADTAMLRS LPFRETVSGL CEVVKNSLAI RPSMVEMLRT
210 220 230 240 250
SLRQDAVYDD ETMYEIISES ILAKASVTVD DMHECRAGLV LEYGHTVGHA
260 270 280 290 300
IEYTAAGGLS HGQAIGLGMV VAAEVSHRLG HLDQEAVALH RELLTRAGAM
310 320 330 340 350
VTIPEEVDLD EVMHRLRFDN KRGYLADPAE SSAMVLLGGL GEPLWHDGRP
360 370 380 390
LVSVPMALVG EVVNEIARPE IPNFELVAPV ETVEEGRVPD TVGAADG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY524043 Genomic DNA Translation: AAR98548.1 AJ575934 Genomic DNA Translation: CAE06511.1 AJ628149 Genomic DNA Translation: CAF31431.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY524043 Genomic DNA Translation: AAR98548.1 AJ575934 Genomic DNA Translation: CAE06511.1 AJ628149 Genomic DNA Translation: CAF31431.1 |
3D structure databases
SMRi | Q70KD0 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00907;UER00921 UPA00967 |
Family and domain databases
InterProi | View protein in InterPro IPR030963, DHQ_synth_fam IPR030960, DHQS/DOIS |
Pfami | View protein in Pfam PF01761, DHQ_synthase, 1 hit |
PIRSFi | PIRSF001455, DHQ_synth, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DOIS_MICEC | |
Accessioni | Q70KD0Primary (citable) accession number: Q70KD0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | October 7, 2020 | |
This is version 48 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families