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UniProtKB - Q70FG7 (DODA_BETVU)
Protein
4,5-DOPA dioxygenase extradiol
Gene
DODA
Organism
Beta vulgaris (Sugar beet)
Status
Functioni
Opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid. Produces mainly (S)-betalamic acid.
1 PublicationMiscellaneous
Supplementation with Fe2+ in the reaction medium is not necessary to detect activity in contrast to the results found for the M. jalapa enzyme (AC B6F0W8) (PubMed:22270561).1 Publication
Catalytic activityi
- EC:1.13.11.291 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Kineticsi
kcat is 0.095 sec(-1) for L-dopa.
- KM=6.9 mM for L-dopa1 Publication
- Vmax=1.2 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
: betalain biosynthesis Pathwayi
This protein is involved in the pathway betalain biosynthesis, which is part of Pigment biosynthesis.View all proteins of this organism that are known to be involved in the pathway betalain biosynthesis and in Pigment biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 15 | ZincBy similarity | 1 | |
Metal bindingi | 53 | ZincBy similarity | 1 | |
Metal bindingi | 175 | ZincBy similarity | 1 | |
Metal bindingi | 229 | ZincBy similarity | 1 |
GO - Molecular functioni
- DOPA dioxygenase activity Source: UniProtKB
- ferrous iron binding Source: InterPro
- stizolobate synthase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular aromatic compound metabolic process Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00278 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DODA |
Organismi | Beta vulgaris (Sugar beet) |
Taxonomic identifieri | 161934 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › Caryophyllales › Chenopodiaceae › Betoideae › Beta |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424076 | 1 – 268 | 4,5-DOPA dioxygenase extradiolAdd BLAST | 268 |
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.Curated
Family and domain databases
CDDi | cd07363, 45_DOPA_Dioxygenase, 1 hit |
InterProi | View protein in InterPro IPR014436, Extradiol_dOase_DODA IPR004183, Xdiol_dOase_suB |
Pfami | View protein in Pfam PF02900, LigB, 1 hit |
PIRSFi | PIRSF006157, Doxgns_DODA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q70FG7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSEDNIKET FFISHGTPMM AIDDSKPSKK FLESWREKIF SKKPKAILVI
60 70 80 90 100
SAHWETDQPS VNVVDINDTI YDFRGFPARL YQFKYSAPGS PELANRIQDL
110 120 130 140 150
LAGSGFKSVN TDKKRGLDHG AWVPLMLMYP EADIPVCQLS VQSHLDGTHH
160 170 180 190 200
YKLGQALAPL KDEGVLIIGS GSATHPSNGT PPCSDGVAPW AAAFDSWLET
210 220 230 240 250
ALTNGSYEEV NKYETKAPNW KLAHPWPEHF YPLHVAMGAA GENSKAELIH
260
NSWDGGIMSY GSYKFTST
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ583017 mRNA Translation: CAE47100.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ583017 mRNA Translation: CAE47100.1 |
3D structure databases
AlphaFoldDBi | Q70FG7 |
SMRi | Q70FG7 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00278 |
Family and domain databases
CDDi | cd07363, 45_DOPA_Dioxygenase, 1 hit |
InterProi | View protein in InterPro IPR014436, Extradiol_dOase_DODA IPR004183, Xdiol_dOase_suB |
Pfami | View protein in Pfam PF02900, LigB, 1 hit |
PIRSFi | PIRSF006157, Doxgns_DODA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DODA_BETVU | |
Accessioni | Q70FG7Primary (citable) accession number: Q70FG7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families