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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Thermus caldophilus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.Curated

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (phi)
  3. ATP-dependent 6-phosphofructokinase (pfk)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49Glyceraldehyde 3-phosphateBy similarity1
Active sitei80Proton donorBy similarity1
Metal bindingi81Zinc 1; catalyticBy similarity1
Metal bindingi102Zinc 2By similarity1
Metal bindingi132Zinc 2By similarity1
Metal bindingi178Zinc 1; catalyticBy similarity1
Binding sitei179Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi208Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi4.1.2.13 6330
UniPathwayi
UPA00109;UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fbaImported
OrganismiThermus caldophilus
Taxonomic identifieri272 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787521 – 305Fructose-bisphosphate aldolaseAdd BLAST305

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ703I2
SMRiQ703I2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ703I2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 211Dihydroxyacetone phosphate bindingBy similarity3
Regioni251 – 254Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Family and domain databases

CDDicd00947 TBP_aldolase_IIB, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR011289 Fruc_bis_ald_class-2
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01859 fruc_bis_ald_, 1 hit

Sequencei

Sequence statusi: Complete.

Q703I2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLVTGLEILR KARAEGYGVG AFNTNNMEFT QAILEAAEEM KSPVILALSE
60 70 80 90 100
GAMKYGGRAL TRMVVALAQE ARVPVAVHLD HGSSYESVLK ALREGFTSVM
110 120 130 140 150
IDKSHEDFET NVRETKRVVE AAHAVGVTVE AELGRLAGIE EHVAVDEKDA
160 170 180 190 200
LLTNPEEARI FMERTGADYL AVAIGTSHGA YKGKGRPFID HPRLARIAKL
210 220 230 240 250
VPAPLVLHGA SAVPQELVER FRAAGGEIGE ASGIHPEDIK KAISLGIAKI
260 270 280 290 300
NTDTDLRLAF TALVRETLGK NPKEFDPRKY LGPAREAVKE VVKSRMELFG

SVGRA
Length:305
Mass (Da):32,987
Last modified:July 5, 2004 - v1
Checksum:i9FE260186F29B98A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ621816 Genomic DNA Translation: CAF32659.1
AY526903 Genomic DNA Translation: AAS19362.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ621816 Genomic DNA Translation: CAF32659.1
AY526903 Genomic DNA Translation: AAS19362.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FJKX-ray2.20A/B/C/D1-305[»]
ProteinModelPortaliQ703I2
SMRiQ703I2
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00183

BRENDAi4.1.2.13 6330

Miscellaneous databases

EvolutionaryTraceiQ703I2

Family and domain databases

CDDicd00947 TBP_aldolase_IIB, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR011289 Fruc_bis_ald_class-2
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01859 fruc_bis_ald_, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALF_THECA
AccessioniPrimary (citable) accession number: Q703I2
Secondary accession number(s): P83739
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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