UniProtKB - Q6ZUT9 (DEN5B_HUMAN)
Protein
DENN domain-containing protein 5B
Gene
DENND5B
Organism
Homo sapiens (Human)
Status
Functioni
Guanine nucleotide exchange factor (GEF) which may activate RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.1 Publication
GO - Molecular functioni
- Rab guanyl-nucleotide exchange factor activity Source: UniProtKB
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
PathwayCommonsi | Q6ZUT9 |
Reactomei | R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
Names & Taxonomyi
Protein namesi | Recommended name: DENN domain-containing protein 5BAlternative name(s): Rab6IP1-like protein |
Gene namesi | Name:DENND5B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28338, DENND5B |
MIMi | 617279, gene |
neXtProti | NX_Q6ZUT9 |
VEuPathDBi | HostDB:ENSG00000170456.14 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Cytosol
- cytosol Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 916 – 936 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 160518 |
OpenTargetsi | ENSG00000170456 |
PharmGKBi | PA164718788 |
Miscellaneous databases
Pharosi | Q6ZUT9, Tbio |
Genetic variation databases
BioMutai | DENND5B |
DMDMi | 182676608 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000326531 | 2 – 1274 | DENN domain-containing protein 5BAdd BLAST | 1273 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 49 | PhosphoserineBy similarity | 1 | |
Modified residuei | 178 | PhosphoserineBy similarity | 1 | |
Modified residuei | 822 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1062 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1068 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1076 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1079 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q6ZUT9 |
jPOSTi | Q6ZUT9 |
MassIVEi | Q6ZUT9 |
MaxQBi | Q6ZUT9 |
PaxDbi | Q6ZUT9 |
PeptideAtlasi | Q6ZUT9 |
PRIDEi | Q6ZUT9 |
ProteomicsDBi | 68365 [Q6ZUT9-1] 68366 [Q6ZUT9-2] 68367 [Q6ZUT9-3] 68368 [Q6ZUT9-4] |
PTM databases
iPTMneti | Q6ZUT9 |
PhosphoSitePlusi | Q6ZUT9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000170456, Expressed in testis and 219 other tissues |
ExpressionAtlasi | Q6ZUT9, baseline and differential |
Genevisiblei | Q6ZUT9, HS |
Organism-specific databases
HPAi | ENSG00000170456, Low tissue specificity |
Interactioni
GO - Molecular functioni
- Rab guanyl-nucleotide exchange factor activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 127761, 9 interactors |
IntActi | Q6ZUT9, 2 interactors |
STRINGi | 9606.ENSP00000373734 |
Miscellaneous databases
RNActi | Q6ZUT9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 244 | uDENNPROSITE-ProRule annotationAdd BLAST | 206 | |
Domaini | 263 – 399 | cDENNPROSITE-ProRule annotationAdd BLAST | 137 | |
Domaini | 401 – 581 | dDENNPROSITE-ProRule annotationAdd BLAST | 181 | |
Domaini | 772 – 932 | RUN 1PROSITE-ProRule annotationAdd BLAST | 161 | |
Domaini | 936 – 1044 | PLATPROSITE-ProRule annotationAdd BLAST | 109 | |
Domaini | 1118 – 1267 | RUN 2PROSITE-ProRule annotationAdd BLAST | 150 |
Sequence similaritiesi
Belongs to the RAB6IP1 family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2080, Eukaryota |
GeneTreei | ENSGT00940000153678 |
HOGENOMi | CLU_004201_2_0_1 |
InParanoidi | Q6ZUT9 |
OrthoDBi | 53600at2759 |
PhylomeDBi | Q6ZUT9 |
TreeFami | TF313237 |
Family and domain databases
Gene3Di | 1.20.58.900, 3 hits 3.40.50.11500, 1 hit |
InterProi | View protein in InterPro IPR001194, cDENN_dom IPR005112, dDENN_dom IPR043153, DENN_C IPR001024, PLAT/LH2_dom IPR036392, PLAT/LH2_dom_sf IPR004012, Run_dom IPR037213, Run_dom_sf IPR037516, Tripartite_DENN IPR005113, uDENN_dom |
Pfami | View protein in Pfam PF03455, dDENN, 1 hit PF02141, DENN, 1 hit PF01477, PLAT, 1 hit PF02759, RUN, 2 hits PF03456, uDENN, 1 hit |
SMARTi | View protein in SMART SM00801, dDENN, 1 hit SM00799, DENN, 1 hit SM00593, RUN, 2 hits SM00800, uDENN, 1 hit |
SUPFAMi | SSF140741, SSF140741, 2 hits SSF49723, SSF49723, 1 hit |
PROSITEi | View protein in PROSITE PS50211, DENN, 1 hit PS50095, PLAT, 1 hit PS50826, RUN, 2 hits |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6ZUT9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGSCAAPGP GSGSSPAACR FAHYFVLCGI DADSGLEPDE LAGENFDQSP
60 70 80 90 100
LRRTFKSKVL AHYPQNIEWN PFDQDAVNML CMPKGLSFRT QTDNKDPQFH
110 120 130 140 150
SFIITREDGS RTYGFVLTFY EEVTSKQICT AMQTLYQMHN AEHYSSVYAS
160 170 180 190 200
SSCSMDSLAS SLDEGDTTSL LKLQRYNSYD ISRDTLYVSK SICLITPLPF
210 220 230 240 250
MQACKKFLIQ LYKAVTSQQP PPLPLESYIH NILYEVPLPP PGRSLKFYGV
260 270 280 290 300
YEPVICQRPG PSELPLSDYP LREAFELLGL ENLVQVFTCV LLEMQILLYS
310 320 330 340 350
QDYQRLMTVA EGITTLLFPF QWQHVYVPIL PASLLHFLDA PVPYLMGLQS
360 370 380 390 400
KEGTDRSKLE LPQEANLCFV DIDNHFIELP EEFPQFPNKV DFIQELSEVL
410 420 430 440 450
VQFGIPPEGS LHCSESTSKL KNMVLKDLVN DKKNGNVCTN NISMYELLKG
460 470 480 490 500
NETIARLQAL AKRTGVAVEK MDLSASLGEK DKDLKLHCEE AELRDYQLNV
510 520 530 540 550
QLREVFANRF TQMFADYEAF VIQTAQDMES WLTNREQMQN FDKASFLSDQ
560 570 580 590 600
PEPYLPFLSR FIETQMFATF IDNKIMSQWE EKDPLLRVFD TRIDKIRLYN
610 620 630 640 650
VRAPTLRTSI YQKCSTLKEA AQSIEQRLMK MDHTAIHPHL LDMKIGQGKY
660 670 680 690 700
EQGFFPKLQS DVLATGPTSN NRWVSRSATA QRRKERLRQH SEHVGLDNDL
710 720 730 740 750
REKYMQEARS LGKNLRQPKL SDLSPAVIAQ TNCKFVEGLL KECRMKTKRM
760 770 780 790 800
LVEKMGHEAV ELGHGEANIT GLEENTLIAS LCDLLERIWS HGLQVKQGKS
810 820 830 840 850
ALWSHLIQFQ DREEKQEHLA ESPVALGPER RKSDSGVMLP TLRVSLIQDM
860 870 880 890 900
RHIQNMSEIK TDVGRARAWI RLSLEKKLLS QHLKQLLSNQ PLTKKLYKRY
910 920 930 940 950
AFLRCEEERE QFLYHLLSLN AVDYFCFTSV FTTIMIPYRS VIIPIKKLSN
960 970 980 990 1000
AIITSNPWIC VSGELGDTGV MQIPKNLLEM TFECQNLGKL TTVQIGHDNS
1010 1020 1030 1040 1050
GLLAKWLVDC VMVRNEITGH TYRFPCGRWL GKGIDDGSLE RILIGELMTS
1060 1070 1080 1090 1100
ASDEDLVKQC RTPPQQKSPT TARRLSITSL TGKNNKPNAG QIQEGIGEAV
1110 1120 1130 1140 1150
NNIVKHFHKP EKERGSLTVL LCGENGLVAA LEQVFHHGFK SARIFHKNVF
1160 1170 1180 1190 1200
IWDFIEKVVA YFETTDQILD NEDDVLIQKS SCKTFCHYVN AINTAPRNIG
1210 1220 1230 1240 1250
KDGKFQILVC LGTRDRLLPQ WIPLLAECPA ITRMYEESAL LRDRMTVNSL
1260 1270
IRILQTIQDF TIVLEGSLIK GVDV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V1S3 | G3V1S3_HUMAN | DENN domain-containing protein 5B | DENND5B MGC24039, hCG_1810950 | 1,309 | Annotation score: | ||
H0YGE5 | H0YGE5_HUMAN | DENN domain-containing protein 5B | DENND5B | 537 | Annotation score: | ||
F5H4A0 | F5H4A0_HUMAN | DENN domain-containing protein 5B | DENND5B | 63 | Annotation score: |
Sequence cautioni
The sequence BAC04583 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 434 | N → D in AAH68580 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 480 | K → R in BAC04583 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 538 | M → T in BAC04583 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 1144 | I → T in BAC04583 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 1149 | V → A in BAC04583 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040076 | 52 | R → K1 PublicationCorresponds to variant dbSNP:rs4930979Ensembl. | 1 | |
Natural variantiVAR_040077 | 487 | H → N2 PublicationsCorresponds to variant dbSNP:rs1056320Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032672 | 1 – 78 | Missing in isoform 3. 1 PublicationAdd BLAST | 78 | |
Alternative sequenceiVSP_032673 | 1 – 42 | MSGSC…PDELA → MGTHHHAQLIFVFLVEMRFH RVDQAGLELLTSGNSPASAS RSAEITVVSQHAQPGFLYQW LEADRHGKSQGAANTTS in isoform 2. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_032674 | 42 | A → AVLYQWLEADRHGKSQGAAN TTS in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_032675 | 544 – 585 | ASFLS…EKDPL → VKRTIVFLLWLLVALLLILT FLNFLFFCAYAYPYIIDFVN LF in isoform 3. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_032676 | 586 – 1274 | Missing in isoform 3. 1 PublicationAdd BLAST | 689 | |
Alternative sequenceiVSP_032677 | 703 – 716 | KYMQE…GKNLR → VCGLPSWGASQQAP in isoform 4. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_032678 | 717 – 1274 | Missing in isoform 4. 1 PublicationAdd BLAST | 558 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK095598 mRNA Translation: BAC04583.1 Different initiation. AK125323 mRNA Translation: BAC86129.1 AC022080 Genomic DNA No translation available. AC068792 Genomic DNA No translation available. CH471116 Genomic DNA Translation: EAW88553.1 BC020855 mRNA Translation: AAH20855.1 BC068580 mRNA Translation: AAH68580.1 AB209341 mRNA Translation: BAD92578.1 BX537924 mRNA Translation: CAD97905.1 CR749639 mRNA Translation: CAH18433.1 |
CCDSi | CCDS44857.1 [Q6ZUT9-1] CCDS76542.1 [Q6ZUT9-2] |
RefSeqi | NP_001295268.1, NM_001308339.1 NP_659410.3, NM_144973.3 [Q6ZUT9-1] |
Genome annotation databases
Ensembli | ENST00000354285; ENSP00000346238; ENSG00000170456 [Q6ZUT9-4] ENST00000389082; ENSP00000373734; ENSG00000170456 [Q6ZUT9-1] |
GeneIDi | 160518 |
KEGGi | hsa:160518 |
UCSCi | uc001rki.2, human [Q6ZUT9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK095598 mRNA Translation: BAC04583.1 Different initiation. AK125323 mRNA Translation: BAC86129.1 AC022080 Genomic DNA No translation available. AC068792 Genomic DNA No translation available. CH471116 Genomic DNA Translation: EAW88553.1 BC020855 mRNA Translation: AAH20855.1 BC068580 mRNA Translation: AAH68580.1 AB209341 mRNA Translation: BAD92578.1 BX537924 mRNA Translation: CAD97905.1 CR749639 mRNA Translation: CAH18433.1 |
CCDSi | CCDS44857.1 [Q6ZUT9-1] CCDS76542.1 [Q6ZUT9-2] |
RefSeqi | NP_001295268.1, NM_001308339.1 NP_659410.3, NM_144973.3 [Q6ZUT9-1] |
3D structure databases
SMRi | Q6ZUT9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 127761, 9 interactors |
IntActi | Q6ZUT9, 2 interactors |
STRINGi | 9606.ENSP00000373734 |
PTM databases
iPTMneti | Q6ZUT9 |
PhosphoSitePlusi | Q6ZUT9 |
Genetic variation databases
BioMutai | DENND5B |
DMDMi | 182676608 |
Proteomic databases
EPDi | Q6ZUT9 |
jPOSTi | Q6ZUT9 |
MassIVEi | Q6ZUT9 |
MaxQBi | Q6ZUT9 |
PaxDbi | Q6ZUT9 |
PeptideAtlasi | Q6ZUT9 |
PRIDEi | Q6ZUT9 |
ProteomicsDBi | 68365 [Q6ZUT9-1] 68366 [Q6ZUT9-2] 68367 [Q6ZUT9-3] 68368 [Q6ZUT9-4] |
Protocols and materials databases
Antibodypediai | 55109, 73 antibodies |
Genome annotation databases
Ensembli | ENST00000354285; ENSP00000346238; ENSG00000170456 [Q6ZUT9-4] ENST00000389082; ENSP00000373734; ENSG00000170456 [Q6ZUT9-1] |
GeneIDi | 160518 |
KEGGi | hsa:160518 |
UCSCi | uc001rki.2, human [Q6ZUT9-1] |
Organism-specific databases
CTDi | 160518 |
DisGeNETi | 160518 |
GeneCardsi | DENND5B |
HGNCi | HGNC:28338, DENND5B |
HPAi | ENSG00000170456, Low tissue specificity |
MIMi | 617279, gene |
neXtProti | NX_Q6ZUT9 |
OpenTargetsi | ENSG00000170456 |
PharmGKBi | PA164718788 |
VEuPathDBi | HostDB:ENSG00000170456.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2080, Eukaryota |
GeneTreei | ENSGT00940000153678 |
HOGENOMi | CLU_004201_2_0_1 |
InParanoidi | Q6ZUT9 |
OrthoDBi | 53600at2759 |
PhylomeDBi | Q6ZUT9 |
TreeFami | TF313237 |
Enzyme and pathway databases
PathwayCommonsi | Q6ZUT9 |
Reactomei | R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
BioGRID-ORCSi | 160518, 6 hits in 875 CRISPR screens |
ChiTaRSi | DENND5B, human |
GenomeRNAii | 160518 |
Pharosi | Q6ZUT9, Tbio |
PROi | PR:Q6ZUT9 |
RNActi | Q6ZUT9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170456, Expressed in testis and 219 other tissues |
ExpressionAtlasi | Q6ZUT9, baseline and differential |
Genevisiblei | Q6ZUT9, HS |
Family and domain databases
Gene3Di | 1.20.58.900, 3 hits 3.40.50.11500, 1 hit |
InterProi | View protein in InterPro IPR001194, cDENN_dom IPR005112, dDENN_dom IPR043153, DENN_C IPR001024, PLAT/LH2_dom IPR036392, PLAT/LH2_dom_sf IPR004012, Run_dom IPR037213, Run_dom_sf IPR037516, Tripartite_DENN IPR005113, uDENN_dom |
Pfami | View protein in Pfam PF03455, dDENN, 1 hit PF02141, DENN, 1 hit PF01477, PLAT, 1 hit PF02759, RUN, 2 hits PF03456, uDENN, 1 hit |
SMARTi | View protein in SMART SM00801, dDENN, 1 hit SM00799, DENN, 1 hit SM00593, RUN, 2 hits SM00800, uDENN, 1 hit |
SUPFAMi | SSF140741, SSF140741, 2 hits SSF49723, SSF49723, 1 hit |
PROSITEi | View protein in PROSITE PS50211, DENN, 1 hit PS50095, PLAT, 1 hit PS50826, RUN, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DEN5B_HUMAN | |
Accessioni | Q6ZUT9Primary (citable) accession number: Q6ZUT9 Secondary accession number(s): B5ME75 Q8WUC8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | April 8, 2008 | |
Last modified: | February 10, 2021 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants