UniProtKB - Q6ZUM4 (RHG27_HUMAN)
Protein
Rho GTPase-activating protein 27
Gene
ARHGAP27
Organism
Homo sapiens (Human)
Status
Functioni
Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).By similarity
Caution
According to HGNC, ARHGAP27 and SH3D20 are 2 separate genes, corresponding to isoform 2 and isoform 4, respectively. However, a rat transcript and paralog proteins with a similar domain structure suggest the existence of a single gene encoding for a protein of 889 residues as displayed here.Curated
GO - Molecular functioni
- GTPase activator activity Source: HGNC-UCL
- SH3 domain binding Source: HGNC-UCL
GO - Biological processi
- positive regulation of GTPase activity Source: HGNC-UCL
- receptor-mediated endocytosis Source: HGNC-UCL
- regulation of GTPase activity Source: GO_Central
- signal transduction Source: InterPro
Keywordsi
Molecular function | GTPase activation |
Biological process | Endocytosis |
Enzyme and pathway databases
PathwayCommonsi | Q6ZUM4 |
Reactomei | R-HSA-194840, Rho GTPase cycle |
SignaLinki | Q6ZUM4 |
SIGNORi | Q6ZUM4 |
Names & Taxonomyi
Protein namesi | Recommended name: Rho GTPase-activating protein 27Alternative name(s): CIN85-associated multi-domain-containing Rho GTPase-activating protein 1 Rho-type GTPase-activating protein 27 SH3 domain-containing protein 20 |
Gene namesi | Name:ARHGAP27 Synonyms:CAMGAP1, SH3D20 ORF Names:PP905 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:31813, ARHGAP27 |
MIMi | 610591, gene |
neXtProti | NX_Q6ZUM4 |
VEuPathDBi | HostDB:ENSG00000159314.11 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Membrane By similarity; Peripheral membrane protein By similarity
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 201176 |
OpenTargetsi | ENSG00000159314 |
PharmGKBi | PA134873327 |
Miscellaneous databases
Pharosi | Q6ZUM4, Tdark |
Genetic variation databases
BioMutai | ARHGAP27 |
DMDMi | 300669680 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317578 | 1 – 889 | Rho GTPase-activating protein 27Add BLAST | 889 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 156 | PhosphoserineBy similarity | 1 | |
Modified residuei | 216 | PhosphoserineBy similarity | 1 | |
Modified residuei | 249 | PhosphoserineCombined sources | 1 | |
Modified residuei | 347 | PhosphoserineBy similarity | 1 | |
Modified residuei | 456 | PhosphoserineBy similarity | 1 | |
Modified residuei | 461 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 466 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6ZUM4 |
jPOSTi | Q6ZUM4 |
MassIVEi | Q6ZUM4 |
MaxQBi | Q6ZUM4 |
PaxDbi | Q6ZUM4 |
PeptideAtlasi | Q6ZUM4 |
PRIDEi | Q6ZUM4 |
ProteomicsDBi | 68341 [Q6ZUM4-1] 68342 [Q6ZUM4-2] 68343 [Q6ZUM4-3] 68344 [Q6ZUM4-4] |
PTM databases
GlyGeni | Q6ZUM4, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q6ZUM4 |
PhosphoSitePlusi | Q6ZUM4 |
Expressioni
Tissue specificityi
Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer.1 Publication
Gene expression databases
Bgeei | ENSG00000159314, Expressed in lower esophagus mucosa and 205 other tissues |
ExpressionAtlasi | Q6ZUM4, baseline and differential |
Genevisiblei | Q6ZUM4, HS |
Organism-specific databases
HPAi | ENSG00000159314, Tissue enhanced (intestine) |
Interactioni
Subunit structurei
Interacts with SH3KBP1/CIN85.
By similarityGO - Molecular functioni
- SH3 domain binding Source: HGNC-UCL
Protein-protein interaction databases
BioGRIDi | 128369, 5 interactors |
IntActi | Q6ZUM4, 2 interactors |
STRINGi | 9606.ENSP00000403323 |
Miscellaneous databases
RNActi | Q6ZUM4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q6ZUM4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 69 | SH3PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 246 – 280 | WW 1PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 299 – 333 | WW 2PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 411 – 444 | WW 3PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 496 – 612 | PHPROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 697 – 886 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 190 |
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG1450, Eukaryota KOG4269, Eukaryota |
GeneTreei | ENSGT00950000182860 |
HOGENOMi | CLU_1140156_0_0_1 |
InParanoidi | Q6ZUM4 |
OMAi | ETVWEDE |
OrthoDBi | 1300981at2759 |
PhylomeDBi | Q6ZUM4 |
TreeFami | TF329345 |
Family and domain databases
CDDi | cd00201, WW, 3 hits |
Gene3Di | 1.10.555.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR015767, ARHGAP27 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR011047, Quinoprotein_ADH-like_supfam IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR23176:SF104, PTHR23176:SF104, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00620, RhoGAP, 1 hit PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00324, RhoGAP, 1 hit SM00456, WW, 3 hits |
SUPFAMi | SSF48350, SSF48350, 1 hit SSF50044, SSF50044, 1 hit SSF50998, SSF50998, 1 hit SSF51045, SSF51045, 2 hits |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50238, RHOGAP, 1 hit PS50002, SH3, 1 hit PS50020, WW_DOMAIN_2, 3 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6ZUM4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAADVVGDVY VLVEHPFEYT GKDGRRVAIR PNERYRLLRR STEHWWHVRR
60 70 80 90 100
EPGGRPFYLP AQYVRELPAL GNPAAAAPPG PHPSPAAPEP LAYDYRFVSA
110 120 130 140 150
AATAGPDGAP EESGGRASSL CGPAQRGAAT QRSSLAPGLP ACLYLRPAAP
160 170 180 190 200
VRPAQSLNDL ACAAVSPPAG LLGSSGSFKA CSVAGSWVCP RPLARSDSEN
210 220 230 240 250
VYEVIQDLHV PPPEESAEQV DDPPEPVYAN IERQPRATSP GAAAAPLPSP
260 270 280 290 300
VWETHTDAGT GRPYYYNPDT GVTTWESPFE AAEGAASPAT SPASVDSHVS
310 320 330 340 350
LETEWGQYWD EESRRVFFYN PLTGETAWED EAENEPEEEL EMQPGLSPGS
360 370 380 390 400
PGDPRPPTPE TDYPESLTSY PEEDYSPVGS FGEPGPTSPL TTPPGWSCHV
410 420 430 440 450
SQDKQMLYTN HFTQEQWVRL EDPHGKPYFY NPEDSSVRWE LPQVPVPAPR
460 470 480 490 500
SIHKSSQDGD TPAQASPPEE KVPAELDEVG SWEEVSPATA AVRTKTLDKA
510 520 530 540 550
GVLHRTKTAD KGKRLRKKHW SASWTVLEGG VLTFFKDSKT SAAGGLRQPS
560 570 580 590 600
KFSTPEYTVE LRGATLSWAP KDKSSRKNVL ELRSRDGSEY LIQHDSEAII
610 620 630 640 650
STWHKAIAQG IQELSAELPP EESESSRVDF GSSERLGSWQ EKEEDARPNA
660 670 680 690 700
AAPALGPVGL ESDLSKVRHK LRKFLQRRPT LQSLREKGYI KDQVFGCALA
710 720 730 740 750
ALCERERSRV PRFVQQCIRA VEARGLDIDG LYRISGNLAT IQKLRYKVDH
760 770 780 790 800
DERLDLDDGR WEDVHVITGA LKLFFRELPE PLFPFSHFRQ FIAAIKLQDQ
810 820 830 840 850
ARRSRCVRDL VRSLPAPNHD TLRMLFQHLC RVIEHGEQNR MSVQSVAIVF
860 870 880
GPTLLRPEVE ETSMPMTMVF QNQVVELILQ QCADIFPPH
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DGE6 | A0A0C4DGE6_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 521 | Annotation score: | ||
A0A0G2JNY3 | A0A0G2JNY3_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 889 | Annotation score: | ||
A0A0G2JRG8 | A0A0G2JRG8_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 867 | Annotation score: | ||
E9PNX6 | E9PNX6_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 214 | Annotation score: | ||
E9PIR1 | E9PIR1_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 867 | Annotation score: | ||
E9PNT2 | E9PNT2_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 667 | Annotation score: | ||
A0A0G2JP16 | A0A0G2JP16_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 263 | Annotation score: | ||
E9PK18 | E9PK18_HUMAN | Rho GTPase-activating protein 27 | ARHGAP27 | 77 | Annotation score: |
Sequence cautioni
The sequence AAI01389 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAI01390 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAI01391 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | S → N in AAG23796 (PubMed:15498874).Curated | 1 | |
Sequence conflicti | 162 | C → R in BAF83339 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 213 | P → R in AAH67345 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_038551 | 889 | H → Q1 PublicationCorresponds to variant dbSNP:rs117139057Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031053 | 1 – 341 | Missing in isoform 2. 1 PublicationAdd BLAST | 341 | |
Alternative sequenceiVSP_031054 | 221 – 263 | DDPPE…GTGRP → PPRALGRGGGWRARDRARTE PGRKETRSAQRRARRPPLSE DFG in isoform 4. 2 PublicationsAdd BLAST | 43 | |
Alternative sequenceiVSP_031055 | 264 – 889 | Missing in isoform 4. 2 PublicationsAdd BLAST | 626 | |
Alternative sequenceiVSP_031056 | 416 – 442 | Missing in isoform 3. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF258593 mRNA Translation: AAG23796.1 AK125535 mRNA Translation: BAC86196.1 AK290650 mRNA Translation: BAF83339.1 AC003070 Genomic DNA No translation available. AC091132 Genomic DNA No translation available. BC067345 mRNA Translation: AAH67345.1 BC101388 mRNA Translation: AAI01389.1 Different initiation. BC101389 mRNA Translation: AAI01390.1 Different initiation. BC101390 mRNA Translation: AAI01391.1 Different initiation. BC101391 mRNA Translation: AAI01392.3 |
CCDSi | CCDS11498.1 [Q6ZUM4-2] CCDS32670.1 [Q6ZUM4-4] CCDS74082.1 [Q6ZUM4-1] |
RefSeqi | NP_001269219.1, NM_001282290.1 [Q6ZUM4-1] NP_777579.2, NM_174919.3 [Q6ZUM4-4] NP_954976.1, NM_199282.2 [Q6ZUM4-2] XP_006721808.1, XM_006721745.2 [Q6ZUM4-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF258593 mRNA Translation: AAG23796.1 AK125535 mRNA Translation: BAC86196.1 AK290650 mRNA Translation: BAF83339.1 AC003070 Genomic DNA No translation available. AC091132 Genomic DNA No translation available. BC067345 mRNA Translation: AAH67345.1 BC101388 mRNA Translation: AAI01389.1 Different initiation. BC101389 mRNA Translation: AAI01390.1 Different initiation. BC101390 mRNA Translation: AAI01391.1 Different initiation. BC101391 mRNA Translation: AAI01392.3 |
CCDSi | CCDS11498.1 [Q6ZUM4-2] CCDS32670.1 [Q6ZUM4-4] CCDS74082.1 [Q6ZUM4-1] |
RefSeqi | NP_001269219.1, NM_001282290.1 [Q6ZUM4-1] NP_777579.2, NM_174919.3 [Q6ZUM4-4] NP_954976.1, NM_199282.2 [Q6ZUM4-2] XP_006721808.1, XM_006721745.2 [Q6ZUM4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3PP2 | X-ray | 1.42 | A/B | 491-613 | [»] | |
SMRi | Q6ZUM4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 128369, 5 interactors |
IntActi | Q6ZUM4, 2 interactors |
STRINGi | 9606.ENSP00000403323 |
PTM databases
GlyGeni | Q6ZUM4, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q6ZUM4 |
PhosphoSitePlusi | Q6ZUM4 |
Genetic variation databases
BioMutai | ARHGAP27 |
DMDMi | 300669680 |
Proteomic databases
EPDi | Q6ZUM4 |
jPOSTi | Q6ZUM4 |
MassIVEi | Q6ZUM4 |
MaxQBi | Q6ZUM4 |
PaxDbi | Q6ZUM4 |
PeptideAtlasi | Q6ZUM4 |
PRIDEi | Q6ZUM4 |
ProteomicsDBi | 68341 [Q6ZUM4-1] 68342 [Q6ZUM4-2] 68343 [Q6ZUM4-3] 68344 [Q6ZUM4-4] |
Protocols and materials databases
Antibodypediai | 49843, 139 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 201176 |
DisGeNETi | 201176 |
GeneCardsi | ARHGAP27 |
HGNCi | HGNC:31813, ARHGAP27 |
HPAi | ENSG00000159314, Tissue enhanced (intestine) |
MIMi | 610591, gene |
neXtProti | NX_Q6ZUM4 |
OpenTargetsi | ENSG00000159314 |
PharmGKBi | PA134873327 |
VEuPathDBi | HostDB:ENSG00000159314.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1450, Eukaryota KOG4269, Eukaryota |
GeneTreei | ENSGT00950000182860 |
HOGENOMi | CLU_1140156_0_0_1 |
InParanoidi | Q6ZUM4 |
OMAi | ETVWEDE |
OrthoDBi | 1300981at2759 |
PhylomeDBi | Q6ZUM4 |
TreeFami | TF329345 |
Enzyme and pathway databases
PathwayCommonsi | Q6ZUM4 |
Reactomei | R-HSA-194840, Rho GTPase cycle |
SignaLinki | Q6ZUM4 |
SIGNORi | Q6ZUM4 |
Miscellaneous databases
BioGRID-ORCSi | 201176, 11 hits in 876 CRISPR screens |
ChiTaRSi | ARHGAP27, human |
GenomeRNAii | 201176 |
Pharosi | Q6ZUM4, Tdark |
PROi | PR:Q6ZUM4 |
RNActi | Q6ZUM4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000159314, Expressed in lower esophagus mucosa and 205 other tissues |
ExpressionAtlasi | Q6ZUM4, baseline and differential |
Genevisiblei | Q6ZUM4, HS |
Family and domain databases
CDDi | cd00201, WW, 3 hits |
Gene3Di | 1.10.555.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR015767, ARHGAP27 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR011047, Quinoprotein_ADH-like_supfam IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR23176:SF104, PTHR23176:SF104, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00620, RhoGAP, 1 hit PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00324, RhoGAP, 1 hit SM00456, WW, 3 hits |
SUPFAMi | SSF48350, SSF48350, 1 hit SSF50044, SSF50044, 1 hit SSF50998, SSF50998, 1 hit SSF51045, SSF51045, 2 hits |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50238, RHOGAP, 1 hit PS50002, SH3, 1 hit PS50020, WW_DOMAIN_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHG27_HUMAN | |
Accessioni | Q6ZUM4Primary (citable) accession number: Q6ZUM4 Secondary accession number(s): A4FU35 Q8WY58 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | July 13, 2010 | |
Last modified: | February 10, 2021 | |
This is version 156 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references