UniProtKB - Q6ZU64 (CFA65_HUMAN)

>sp|Q6ZU64|CFA65_HUMAN Cilia- and flagella-associated protein 65 OS=Homo sapiens OX=9606 GN=CFAP65 PE=1 SV=2
MFTLTGCRLVEKTQKVENPSVSFASSFPLIPLLLRGKSVQKKQAESKSQIKLHTQSAPFG
LCPKDMMLTQAPSSVVRSRNSRNHTVNSGGSCLSASTVAIPAINDSSAAMSACSTISAQP
ASSMDTQMHSPKKQERVNKRVIWGIEVAEELHWKGWELGKETTRNLVLKNRSLKLQKMKY
RPPKTKFFFTVIPQPIFLSPGITLTLPIVFRPLEAKEYMDQLWFEKAEGMFCVGLRATLP
CHRLICRPPSLQLPMCAVGDTTEAFFCLDNVGDLPTFFTWEFSSPFQMLPATGLLEPGQA
SQIKVTFQPLTAVIYEVQATCWYGAGSRQRSSIQLQAVAKCAQLLVSIKHKCPEDQDAEG
FQKLLYFGSVAVGCTSERQIRLHNPSAVNAPFRIEISPDELAEDQAFSCPTAHGIVLPGE
KKCVSVFFHPKTLDTRTVDYCSIMPSGCASKTLLKVVGFCRGPAVSLQHYCVNFSWVNLG
ERSEQPLWIENQSDCTAHFQFAIDCLESVFTIRPAFGTLVGKARMTLHCAFQPTHPIICF
RRVACLIHHQDPLFLDLMGTCHSDSTKPAILKPQHLTWYRTHLARGLTLYPPDILDAMLK
EKKLAQDQNGALMIPIQDLEDMPAPQYPYIPPMTEFFFDGTSDITIFPPPISVEPVEVDF
GACPGPEAPNPVPLCLMNHTKGKIMVVWTRRSDCPFWVTPESCDVPPLKSMAMRLHFQPP
HPNCLYTVELEAFAIYKVLQSYSNIEEDCTMCPSWCLTVRARGHSYFAGFEHHIPQYSLD
VPKLFPAVSSGEPTYRSLLLVNKDCKLLTFSLAPQRGSDVILRPTSGLVAPGAHQIILIC
TYPEGSSWKQHTFYLQCNASPQYLKEVSMYSREEPLQLKLDTHKSLYFKPTWVGCSSTSP
FTFRNPSRLPLQFEWRVSEQHRKLLAVQPSRGLIQPNERLTLTWTFSPLEETKYLFQVGM
WVWEAGLSPNANPAATTHYMLRLVGVGLTSSLSAKEKELAFGNVLVNSKQSRFLVLLNDG
NCTLYYRLYLEQGSPEAVDNHPLALQLDRTEGSMPPRSQDTICLTACPKQRSQYSWTITY
SLLSHRDNKAGEKQELCCVSLVAVYPLLSILDVSSMGSAEGITRKHLWRLFSLDLLNSYL
ERDPTPCELTYKVPTRHSMSQIPPVLTPLRLDFNFGAAPFKAPPSVVFLALKNSGVVSLD
WAFLLPSDQRIDVELWAEQAELNSTELHQMRVQDNCLFSISPKAGSLSPGQEQMVELKYS
HLFIGTDHLPVLFKVSHGREILLNFIGVTVKPEQKYVHFTSTTHQFIPIPIGDTLPPRQI
YELYNGGSVPVTYEVQTDVLSQVQEKNFDHPIFCCLNPKGEIQPGSTARVLWIFSPIEAK
TYTVDVPIHILGWNSALIHFQGVGYNPHMMGDTAPFHNISSWDNSSIHSRLVVPGQNVFL
SQSHISLGNIPVQSKCSRLLFLNNISKNEEIAFSWQPSPLDFGEVSVSPMIGVVAPEETV
PFVVTLRASVHASFYSADLVCKLYSQQLMRQYHKELQEWKDEKVRQEVEFTITDMKVKKR
TCCTACEPARKYKTLPPIKNQQSVSRPASWKLQTPKEEVSWPCPQPPSPGMLCLGLTARA
HATDYFLANFFSEFPCHFLHRELPKRKAPREESETSEEKSPNKWGPVSKQKKQLLVDILT
TIIRGLLEDKNFHEAVDQSLVEQVPYFRQFWNEQSTKFMDQKNSLYLMPILPVPSSSWED
GKGKQPKEDRPEHYPGLGKKEEGEEEKGEEEEEELEEEEEEEEETEEEELGKEEIEEKEE
ERDEKEEKVSWAGIGPTPQPESQESMQWQWQQQLNVMVKEEQEQDEKEAIRRLPAFANLQ
EALLENMIQNILVEASRGEVVLTSRPRVIALPPFCVPRSLTPDTLLPTQQAEVLHPVVPL
PTDLP

Entry version 129 (02 Dec 2020)
Sequence version 2 (29 May 2007)
Previous versions | rss

Add a publication Feedback

Protein

Cilia- and flagella-associated protein 65

Gene

CFAP65

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Plays a role in flagellar formation and sperm motility.By similarity

GO - Molecular functionⁱ

RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- "Insights into RNA biology from an atlas of mammalian mRNA-binding proteins."
  Castello A., Fischer B., Eichelbaum K., Horos R., Beckmann B.M., Strein C., Davey N.E., Humphreys D.T., Preiss T., Steinmetz L.M., Krijgsveld J., Hentze M.W.
  Cell 149:1393-1406(2012) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

flagellated sperm motility Source: UniProtKB
sperm axoneme assembly Source: UniProtKB

Complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cilium biogenesis/degradation

Biological process

Cilium biogenesis/degradation

Enzyme and pathway databases

PathwayCommonsⁱ	Q6ZU64

PathwayCommonsⁱ

Q6ZU64

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cilia- and flagella-associated protein 65Curated Alternative name(s): Coiled-coil domain-containing protein 108Imported
Gene namesⁱ	Name:CFAP65Imported Synonyms:CCDC108Imported
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000181378.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:25325, CFAP65
MIMⁱ	614270, gene
neXtProtⁱ	NX_Q6ZU64

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	188 – 208	HelicalSequence analysisAdd BLAST		21

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Flagellum, Membrane

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Spermatogenic failure 40 (SPGF40)4 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal recessive infertility disorder characterized by severely reduced or absent sperm motility, due to multiple morphologic anomalies such as absent, short, bent, coiled and irregular-caliber tails. Patient spermatozoa may also show morphologic defects of the sperm head.

Related information in OMIM

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_083169	762 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	1164
Natural variantⁱVAR_083170	892 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	1034
Natural variantⁱVAR_083171	1007	N → K in SPGF40; unknown pathological significance. 1 Publication	1
Natural variantⁱVAR_083172	1016	L → R in SPGF40; unknown pathological significance. 1 Publication	1
Natural variantⁱVAR_083173	1619 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	307
Natural variantⁱVAR_083174	1757 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	169
Natural variantⁱVAR_080903	1781 – 1925	Missing in SPGF40. 3 PublicationsAdd BLAST	145

Keywords - Diseaseⁱ

Disease mutation

Organism-specific databases

DisGeNET More...DisGeNETⁱ	255101
MalaCards human disease database More...MalaCardsⁱ	CFAP65
MIMⁱ	618664, phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000181378
Orphanetⁱ	276234, Non-syndromic male infertility due to sperm motility disorder
PharmGKBⁱ	PA145008681

Miscellaneous databases

Pharosⁱ	Q6ZU64, Tdark

Pharosⁱ

Q6ZU64, Tdark

Polymorphism and mutation databases

BioMutaⁱ	CFAP65
DMDMⁱ	148841209

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000288805	1 – 1925	Cilia- and flagella-associated protein 65Add BLAST		1925

Proteomic databases

EPDⁱ	Q6ZU64
MassIVEⁱ	Q6ZU64
PaxDbⁱ	Q6ZU64
PeptideAtlas More...PeptideAtlasⁱ	Q6ZU64
PRIDEⁱ	Q6ZU64
ProteomicsDBⁱ	19496 20228 20258 68312 [Q6ZU64-1]

PTM databases

iPTMnetⁱ	Q6ZU64
PhosphoSitePlusⁱ	Q6ZU64

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000181378, Expressed in right uterine tube and 99 other tissues
ExpressionAtlasⁱ	Q6ZU64, baseline and differential
Genevisibleⁱ	Q6ZU64, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000181378, Tissue enhanced (brain, fallopian tube, testis)

HPAⁱ

ENSG00000181378, Tissue enhanced (brain, fallopian tube, testis)

Interactionⁱ

Binary interactionsⁱ

Isoform 2 [Q6ZU64-3]

With	#Exp.	IntAct
CCNDBP1 [O95273]	3	EBI-10255250,EBI-748961

Protein-protein interaction databases

BioGRIDⁱ	129075, 2 interactors
IntActⁱ	Q6ZU64, 3 interactors
STRINGⁱ	9606.ENSP00000340776

Miscellaneous databases

RNActⁱ	Q6ZU64, protein

RNActⁱ

Q6ZU64, protein

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	877 – 986	MSPPROSITE-ProRule annotationAdd BLAST		110

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	1525 – 1550	Sequence analysisAdd BLAST		26

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	1761 – 1848	Glu-richAdd BLAST		88

Sequence similaritiesⁱ

Belongs to the CFAP65 family.Curated

Keywords - Domainⁱ

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG502QSJW, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00430000031142
HOGENOMⁱ	CLU_000944_1_0_1
InParanoidⁱ	Q6ZU64
OMAⁱ	TICLTAC
Database of Orthologous Groups More...OrthoDBⁱ	1127899at2759
PhylomeDBⁱ	Q6ZU64
TreeFamⁱ	TF329056

Family and domain databases

Gene3Dⁱ	2.60.40.10, 7 hits
InterProⁱ	View protein in InterPro IPR013783, Ig-like_fold IPR000535, MSP_dom
PROSITEⁱ	View protein in PROSITE PS50202, MSP, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 5 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q6ZU64-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFTLTGCRLV EKTQKVENPS VSFASSFPLI PLLLRGKSVQ KKQAESKSQI 
        60         70         80         90        100
KLHTQSAPFG LCPKDMMLTQ APSSVVRSRN SRNHTVNSGG SCLSASTVAI 
       110        120        130        140        150
PAINDSSAAM SACSTISAQP ASSMDTQMHS PKKQERVNKR VIWGIEVAEE 
       160        170        180        190        200
LHWKGWELGK ETTRNLVLKN RSLKLQKMKY RPPKTKFFFT VIPQPIFLSP 
       210        220        230        240        250
GITLTLPIVF RPLEAKEYMD QLWFEKAEGM FCVGLRATLP CHRLICRPPS 
       260        270        280        290        300
LQLPMCAVGD TTEAFFCLDN VGDLPTFFTW EFSSPFQMLP ATGLLEPGQA 
       310        320        330        340        350
SQIKVTFQPL TAVIYEVQAT CWYGAGSRQR SSIQLQAVAK CAQLLVSIKH 
       360        370        380        390        400
KCPEDQDAEG FQKLLYFGSV AVGCTSERQI RLHNPSAVNA PFRIEISPDE 
       410        420        430        440        450
LAEDQAFSCP TAHGIVLPGE KKCVSVFFHP KTLDTRTVDY CSIMPSGCAS 
       460        470        480        490        500
KTLLKVVGFC RGPAVSLQHY CVNFSWVNLG ERSEQPLWIE NQSDCTAHFQ 
       510        520        530        540        550
FAIDCLESVF TIRPAFGTLV GKARMTLHCA FQPTHPIICF RRVACLIHHQ 
       560        570        580        590        600
DPLFLDLMGT CHSDSTKPAI LKPQHLTWYR THLARGLTLY PPDILDAMLK 
       610        620        630        640        650
EKKLAQDQNG ALMIPIQDLE DMPAPQYPYI PPMTEFFFDG TSDITIFPPP 
       660        670        680        690        700
ISVEPVEVDF GACPGPEAPN PVPLCLMNHT KGKIMVVWTR RSDCPFWVTP 
       710        720        730        740        750
ESCDVPPLKS MAMRLHFQPP HPNCLYTVEL EAFAIYKVLQ SYSNIEEDCT 
       760        770        780        790        800
MCPSWCLTVR ARGHSYFAGF EHHIPQYSLD VPKLFPAVSS GEPTYRSLLL 
       810        820        830        840        850
VNKDCKLLTF SLAPQRGSDV ILRPTSGLVA PGAHQIILIC TYPEGSSWKQ 
       860        870        880        890        900
HTFYLQCNAS PQYLKEVSMY SREEPLQLKL DTHKSLYFKP TWVGCSSTSP 
       910        920        930        940        950
FTFRNPSRLP LQFEWRVSEQ HRKLLAVQPS RGLIQPNERL TLTWTFSPLE 
       960        970        980        990       1000
ETKYLFQVGM WVWEAGLSPN ANPAATTHYM LRLVGVGLTS SLSAKEKELA 
      1010       1020       1030       1040       1050
FGNVLVNSKQ SRFLVLLNDG NCTLYYRLYL EQGSPEAVDN HPLALQLDRT 
      1060       1070       1080       1090       1100
EGSMPPRSQD TICLTACPKQ RSQYSWTITY SLLSHRDNKA GEKQELCCVS 
      1110       1120       1130       1140       1150
LVAVYPLLSI LDVSSMGSAE GITRKHLWRL FSLDLLNSYL ERDPTPCELT 
      1160       1170       1180       1190       1200
YKVPTRHSMS QIPPVLTPLR LDFNFGAAPF KAPPSVVFLA LKNSGVVSLD 
      1210       1220       1230       1240       1250
WAFLLPSDQR IDVELWAEQA ELNSTELHQM RVQDNCLFSI SPKAGSLSPG 
      1260       1270       1280       1290       1300
QEQMVELKYS HLFIGTDHLP VLFKVSHGRE ILLNFIGVTV KPEQKYVHFT 
      1310       1320       1330       1340       1350
STTHQFIPIP IGDTLPPRQI YELYNGGSVP VTYEVQTDVL SQVQEKNFDH 
      1360       1370       1380       1390       1400
PIFCCLNPKG EIQPGSTARV LWIFSPIEAK TYTVDVPIHI LGWNSALIHF 
      1410       1420       1430       1440       1450
QGVGYNPHMM GDTAPFHNIS SWDNSSIHSR LVVPGQNVFL SQSHISLGNI 
      1460       1470       1480       1490       1500
PVQSKCSRLL FLNNISKNEE IAFSWQPSPL DFGEVSVSPM IGVVAPEETV 
      1510       1520       1530       1540       1550
PFVVTLRASV HASFYSADLV CKLYSQQLMR QYHKELQEWK DEKVRQEVEF 
      1560       1570       1580       1590       1600
TITDMKVKKR TCCTACEPAR KYKTLPPIKN QQSVSRPASW KLQTPKEEVS 
      1610       1620       1630       1640       1650
WPCPQPPSPG MLCLGLTARA HATDYFLANF FSEFPCHFLH RELPKRKAPR 
      1660       1670       1680       1690       1700
EESETSEEKS PNKWGPVSKQ KKQLLVDILT TIIRGLLEDK NFHEAVDQSL 
      1710       1720       1730       1740       1750
VEQVPYFRQF WNEQSTKFMD QKNSLYLMPI LPVPSSSWED GKGKQPKEDR 
      1760       1770       1780       1790       1800
PEHYPGLGKK EEGEEEKGEE EEEELEEEEE EEEETEEEEL GKEEIEEKEE 
      1810       1820       1830       1840       1850
ERDEKEEKVS WAGIGPTPQP ESQESMQWQW QQQLNVMVKE EQEQDEKEAI 
      1860       1870       1880       1890       1900
RRLPAFANLQ EALLENMIQN ILVEASRGEV VLTSRPRVIA LPPFCVPRSL 
      1910       1920 
TPDTLLPTQQ AEVLHPVVPL PTDLP

Length:1,925

Mass (Da):217,250

Last modified:May 29, 2007 - v2

Checksum:ⁱ000CFE2B83394B42

Isoform 2 (identifier: Q6ZU64-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.
     182-229: PPKTKFFFTV...DQLWFEKAEG → YQYKGSRTQC...SECLKYVQYE
     230-1925: Missing.

« Hide

        10         20         30         40         50
MMLTQAPSSV VRSRNSRNHT VNSGGSCLSA STVAIPAIND SSAAMSACST 
        60         70         80         90        100
ISAQPASSMD TQMHSPKKQE RVNKRVIWGI EVAEELHWKG WELGKETTRN 
       110        120        130        140        150
LVLKNRSLKL QKMKYRYQYK GSRTQCHSLE PRKQALFKTK QNKQKKPLTC 
       160 
HIKASECLKY VQYE

Show »

Length:164

Mass (Da):18,571

Checksum:ⁱE61DB5602ADD73C2

Isoform 3 (identifier: Q6ZU64-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.
     739-795: LQSYSNIEED...PAVSSGEPTY → CARNEREECG...MSLSPPKLLA
     796-1925: Missing.

« Hide

        10         20         30         40         50
MMLTQAPSSV VRSRNSRNHT VNSGGSCLSA STVAIPAIND SSAAMSACST 
        60         70         80         90        100
ISAQPASSMD TQMHSPKKQE RVNKRVIWGI EVAEELHWKG WELGKETTRN 
       110        120        130        140        150
LVLKNRSLKL QKMKYRPPKT KFFFTVIPQP IFLSPGITLT LPIVFRPLEA 
       160        170        180        190        200
KEYMDQLWFE KAEGMFCVGL RATLPCHRLI CRPPSLQLPM CAVGDTTEAF 
       210        220        230        240        250
FCLDNVGDLP TFFTWEFSSP FQMLPATGLL EPGQASQIKV TFQPLTAVIY 
       260        270        280        290        300
EVQATCWYGA GSRQRSSIQL QAVAKCAQLL VSIKHKCPED QDAEGFQKLL 
       310        320        330        340        350
YFGSVAVGCT SERQIRLHNP SAVNAPFRIE ISPDELAEDQ AFSCPTAHGI 
       360        370        380        390        400
VLPGEKKCVS VFFHPKTLDT RTVDYCSIMP SGCASKTLLK VVGFCRGPAV 
       410        420        430        440        450
SLQHYCVNFS WVNLGERSEQ PLWIENQSDC TAHFQFAIDC LESVFTIRPA 
       460        470        480        490        500
FGTLVGKARM TLHCAFQPTH PIICFRRVAC LIHHQDPLFL DLMGTCHSDS 
       510        520        530        540        550
TKPAILKPQH LTWYRTHLAR GLTLYPPDIL DAMLKEKKLA QDQNGALMIP 
       560        570        580        590        600
IQDLEDMPAP QYPYIPPMTE FFFDGTSDIT IFPPPISVEP VEVDFGACPG 
       610        620        630        640        650
PEAPNPVPLC LMNHTKGKIM VVWTRRSDCP FWVTPESCDV PPLKSMAMRL 
       660        670        680        690        700
HFQPPHPNCL YTVELEAFAI YKVCARNERE ECGVSARSLS GLVGWQEVTE 
       710        720        730
GSFRLHPLRA RLSLGWTVTP MSLSPPKLLA

Show »

Length:730

Mass (Da):81,378

Checksum:ⁱF42999BD58A1ACC8

Isoform 4 (identifier: Q6ZU64-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MFTLTGCRLVEKTQKVENPSVSFASSFPLIPLLL → METAIWRRSYISLISSERPVHNW
     739-795: LQSYSNIEED...PAVSSGEPTY → CARNEREECG...MSLSPPKLLA
     796-1925: Missing.

« Hide

        10         20         30         40         50
METAIWRRSY ISLISSERPV HNWRGKSVQK KQAESKSQIK LHTQSAPFGL 
        60         70         80         90        100
CPKDMMLTQA PSSVVRSRNS RNHTVNSGGS CLSASTVAIP AINDSSAAMS 
       110        120        130        140        150
ACSTISAQPA SSMDTQMHSP KKQERVNKRV IWGIEVAEEL HWKGWELGKE 
       160        170        180        190        200
TTRNLVLKNR SLKLQKMKYR PPKTKFFFTV IPQPIFLSPG ITLTLPIVFR 
       210        220        230        240        250
PLEAKEYMDQ LWFEKAEGMF CVGLRATLPC HRLICRPPSL QLPMCAVGDT 
       260        270        280        290        300
TEAFFCLDNV GDLPTFFTWE FSSPFQMLPA TGLLEPGQAS QIKVTFQPLT 
       310        320        330        340        350
AVIYEVQATC WYGAGSRQRS SIQLQAVAKC AQLLVSIKHK CPEDQDAEGF 
       360        370        380        390        400
QKLLYFGSVA VGCTSERQIR LHNPSAVNAP FRIEISPDEL AEDQAFSCPT 
       410        420        430        440        450
AHGIVLPGEK KCVSVFFHPK TLDTRTVDYC SIMPSGCASK TLLKVVGFCR 
       460        470        480        490        500
GPAVSLQHYC VNFSWVNLGE RSEQPLWIEN QSDCTAHFQF AIDCLESVFT 
       510        520        530        540        550
IRPAFGTLVG KARMTLHCAF QPTHPIICFR RVACLIHHQD PLFLDLMGTC 
       560        570        580        590        600
HSDSTKPAIL KPQHLTWYRT HLARGLTLYP PDILDAMLKE KKLAQDQNGA 
       610        620        630        640        650
LMIPIQDLED MPAPQYPYIP PMTEFFFDGT SDITIFPPPI SVEPVEVDFG 
       660        670        680        690        700
ACPGPEAPNP VPLCLMNHTK GKIMVVWTRR SDCPFWVTPE SCDVPPLKSM 
       710        720        730        740        750
AMRLHFQPPH PNCLYTVELE AFAIYKVCAR NEREECGVSA RSLSGLVGWQ 
       760        770        780 
EVTEGSFRLH PLRARLSLGW TVTPMSLSPP KLLA

Show »

Length:784

Mass (Da):87,601

Checksum:ⁱ15CFF1980112F460

Computationally mapped potential isoform sequencesⁱ

There are 5 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

C9JVA0	C9JVA0_HUMAN	Cilia- and flagella-associated prot... Cilia- and flagella-associated protein 65	CFAP65	234	Annotation score:
Q49A14	Q49A14_HUMAN	CCDC108 protein CCDC108 protein (Cilia- and flagella-associated protein 65)	CFAP65 CCDC108	271	Annotation score:
C9JIV0	C9JIV0_HUMAN	Cilia- and flagella-associated prot... Cilia- and flagella-associated protein 65	CFAP65	117	Annotation score:
C9JLP9	C9JLP9_HUMAN	Cilia- and flagella-associated prot... Cilia- and flagella-associated protein 65	CFAP65	114	Annotation score:
H7C0B4	H7C0B4_HUMAN	Cilia- and flagella-associated prot... Cilia- and flagella-associated protein 65	CFAP65	127	Annotation score:

Sequence cautionⁱ

The sequence BAC86362 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated

The sequence BAC86878 differs from that shown. Unlikely isoform. Aberrant splice sites.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	129	H → R in BAC86878 (PubMed:14702039).Curated	1
Sequence conflictⁱ	320	T → A in BAC86878 (PubMed:14702039).Curated	1
Sequence conflictⁱ	665	G → W in BAC86878 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_032500	66	M → L2 PublicationsCorresponds to variant dbSNP:rs6736922Ensembl.	1
Natural variantⁱVAR_083656	639	D → E1 Publication	1
Natural variantⁱVAR_050726	672	V → I. Corresponds to variant dbSNP:rs13403802Ensembl.	1
Natural variantⁱVAR_083169	762 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	1164
Natural variantⁱVAR_050727	806	K → M. Corresponds to variant dbSNP:rs9653262Ensembl.	1
Natural variantⁱVAR_083170	892 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	1034
Natural variantⁱVAR_083171	1007	N → K in SPGF40; unknown pathological significance. 1 Publication	1
Natural variantⁱVAR_083172	1016	L → R in SPGF40; unknown pathological significance. 1 Publication	1
Natural variantⁱVAR_083657	1240	I → F1 Publication	1
Natural variantⁱVAR_083173	1619 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	307
Natural variantⁱVAR_083174	1757 – 1925	Missing in SPGF40. 1 PublicationAdd BLAST	169
Natural variantⁱVAR_080903	1781 – 1925	Missing in SPGF40. 3 PublicationsAdd BLAST	145
Natural variantⁱVAR_050728	1858	N → S. Corresponds to variant dbSNP:rs11893183Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_045221	1 – 65	Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST	65
Alternative sequenceⁱVSP_055631	1 – 34	MFTLT…IPLLL → METAIWRRSYISLISSERPV HNW in isoform 4. CuratedAdd BLAST	34
Alternative sequenceⁱVSP_045222	182 – 229	PPKTK…EKAEG → YQYKGSRTQCHSLEPRKQAL FKTKQNKQKKPLTCHIKASE CLKYVQYE in isoform 2. 1 PublicationAdd BLAST	48
Alternative sequenceⁱVSP_045223	230 – 1925	Missing in isoform 2. 1 PublicationAdd BLAST	1696
Alternative sequenceⁱVSP_055632	739 – 795	LQSYS…GEPTY → CARNEREECGVSARSLSGLV GWQEVTEGSFRLHPLRARLS LGWTVTPMSLSPPKLLA in isoform 3 and isoform 4. CuratedAdd BLAST	57
Alternative sequenceⁱVSP_055633	796 – 1925	Missing in isoform 3 and isoform 4. CuratedAdd BLAST	1130

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK125957 mRNA Translation: BAC86362.1 Different initiation. AK127189 mRNA Translation: BAC86878.1 Sequence problems. AC097468 Genomic DNA No translation available. BC031585 mRNA Translation: AAH31585.1 BC130281 mRNA Translation: AAI30282.1 AL833882 mRNA Translation: CAD38738.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS2430.2 [Q6ZU64-1] CCDS2431.2 [Q6ZU64-3] CCDS63125.1 [Q6ZU64-4] CCDS63126.1 [Q6ZU64-5]
NCBI Reference Sequences More...RefSeqⁱ	NP_689602.2, NM_152389.3 [Q6ZU64-3] NP_919278.2, NM_194302.3 [Q6ZU64-1]

Genome annotation databases

Ensemblⁱ	ENST00000295729; ENSP00000295729; ENSG00000181378 [Q6ZU64-3] ENST00000341552; ENSP00000340776; ENSG00000181378 [Q6ZU64-1] ENST00000409865; ENSP00000386945; ENSG00000181378 [Q6ZU64-5] ENST00000410037; ENSP00000386258; ENSG00000181378 [Q6ZU64-4] ENST00000453220; ENSP00000409117; ENSG00000181378 [Q6ZU64-1]
GeneIDⁱ	255101
KEGGⁱ	hsa:255101
UCSC genome browser More...UCSCⁱ	uc002vjl.3, human [Q6ZU64-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q6ZU64-3	Uncharacterized protein MGC35338		HUMAN		163	UniRef100_Q4ZFW9

Protein	Similar proteins		Species	Score	Length	Source
Q6ZU64	CFAP65 isoform 1		PONAB		1926	UniRef90_Q6ZU64
CFAP65 isoform 10		PANTR		1925
Cilia and flagella associated protein 65		GORGO		1925
Uncharacterized protein		Cebus capucinus imitator		1914
Uncharacterized protein		AOTNA		1907
+21
Q6ZU64-3	Cilia and flagella associated protein 65		PANTR		163	UniRef90_A0A2I3T000
Uncharacterized protein		PANPA		163
CFAP65 isoform 12		PONAB		164
Uncharacterized protein		RHIRO		163
Uncharacterized protein		RHIBE		163
+3
Q6ZU64-4	Isoform 4 of Cilia- and flagella-associated protein 65		HUMAN		784	UniRef90_Q6ZU64-5
CFAP65 isoform 8 (Fragment)		PONAB		785
Uncharacterized protein		PANPA		784
ASH domain-containing protein		NOMLE		784
CFAP65 isoform 7		PANTR		784
+29

Protein	Similar proteins		Species	Score	Length	Source
Q6ZU64	Cilia- and flagella-associated protein 65		MOUSE		1847	UniRef50_Q6ZU64
MSP domain-containing protein		PONAB		1926
Cilia and flagella associated protein 65		PANTR		1925
Cilia and flagella associated protein 65		GORGO		1925
Uncharacterized protein		Cebus capucinus imitator		1914
+353
Q6ZU64-3	Cilia and flagella associated protein 65		PANTR		163	UniRef50_A0A2I3T000
Uncharacterized protein		PANPA		163
CFAP65 isoform 12		PONAB		164
Uncharacterized protein		NOMLE		163
Cilia and flagella associated protein 65		GORGO		163
+3
Q6ZU64-4	Isoform 4 of Cilia- and flagella-associated protein 65		HUMAN		784	UniRef50_Q6ZU64-5
CFAP65 isoform 8 (Fragment)		PONAB		785
Cilia and flagella associated protein 65		PANTR		784
ASH domain-containing protein		COLAP		784
ASH domain-containing protein		NOMLE		784
+38

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK125957 mRNA Translation: BAC86362.1 Different initiation. AK127189 mRNA Translation: BAC86878.1 Sequence problems. AC097468 Genomic DNA No translation available. BC031585 mRNA Translation: AAH31585.1 BC130281 mRNA Translation: AAI30282.1 AL833882 mRNA Translation: CAD38738.1
CCDSⁱ	CCDS2430.2 [Q6ZU64-1] CCDS2431.2 [Q6ZU64-3] CCDS63125.1 [Q6ZU64-4] CCDS63126.1 [Q6ZU64-5]
RefSeqⁱ	NP_689602.2, NM_152389.3 [Q6ZU64-3] NP_919278.2, NM_194302.3 [Q6ZU64-1]

3D structure databases

ModBaseⁱ	Search...
SWISS-MODEL-Workspaceⁱ	Submit a new modelling project...

Protein-protein interaction databases

BioGRIDⁱ	129075, 2 interactors
IntActⁱ	Q6ZU64, 3 interactors
STRINGⁱ	9606.ENSP00000340776

PTM databases

iPTMnetⁱ	Q6ZU64
PhosphoSitePlusⁱ	Q6ZU64

Polymorphism and mutation databases

BioMutaⁱ	CFAP65
DMDMⁱ	148841209

Proteomic databases

EPDⁱ	Q6ZU64
MassIVEⁱ	Q6ZU64
PaxDbⁱ	Q6ZU64
PeptideAtlasⁱ	Q6ZU64
PRIDEⁱ	Q6ZU64
ProteomicsDBⁱ	19496 20228 20258 68312 [Q6ZU64-1]

Protocols and materials databases

Antibodypediaⁱ	34298, 37 antibodies
The DNASU plasmid repository More...DNASUⁱ	255101

Genome annotation databases

Ensemblⁱ	ENST00000295729; ENSP00000295729; ENSG00000181378 [Q6ZU64-3] ENST00000341552; ENSP00000340776; ENSG00000181378 [Q6ZU64-1] ENST00000409865; ENSP00000386945; ENSG00000181378 [Q6ZU64-5] ENST00000410037; ENSP00000386258; ENSG00000181378 [Q6ZU64-4] ENST00000453220; ENSP00000409117; ENSG00000181378 [Q6ZU64-1]
GeneIDⁱ	255101
KEGGⁱ	hsa:255101
UCSCⁱ	uc002vjl.3, human [Q6ZU64-1]

Organism-specific databases

CTDⁱ	255101
DisGeNETⁱ	255101
EuPathDBⁱ	HostDB:ENSG00000181378.13
GeneCardsⁱ	CFAP65
HGNCⁱ	HGNC:25325, CFAP65
HPAⁱ	ENSG00000181378, Tissue enhanced (brain, fallopian tube, testis)
MalaCardsⁱ	CFAP65
MIMⁱ	614270, gene 618664, phenotype
neXtProtⁱ	NX_Q6ZU64
OpenTargetsⁱ	ENSG00000181378
Orphanetⁱ	276234, Non-syndromic male infertility due to sperm motility disorder
PharmGKBⁱ	PA145008681
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG502QSJW, Eukaryota
GeneTreeⁱ	ENSGT00430000031142
HOGENOMⁱ	CLU_000944_1_0_1
InParanoidⁱ	Q6ZU64
OMAⁱ	TICLTAC
OrthoDBⁱ	1127899at2759
PhylomeDBⁱ	Q6ZU64
TreeFamⁱ	TF329056

Enzyme and pathway databases

PathwayCommonsⁱ	Q6ZU64

PathwayCommonsⁱ

Q6ZU64

Miscellaneous databases

BioGRID-ORCSⁱ	255101, 2 hits in 848 CRISPR screens
ChiTaRSⁱ	CFAP65, human
GenomeRNAiⁱ	255101
Pharosⁱ	Q6ZU64, Tdark
Protein Ontology More...PROⁱ	PR:Q6ZU64
RNActⁱ	Q6ZU64, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000181378, Expressed in right uterine tube and 99 other tissues
ExpressionAtlasⁱ	Q6ZU64, baseline and differential
Genevisibleⁱ	Q6ZU64, HS

Family and domain databases

Gene3Dⁱ	2.60.40.10, 7 hits
InterProⁱ	View protein in InterPro IPR013783, Ig-like_fold IPR000535, MSP_dom
PROSITEⁱ	View protein in PROSITE PS50202, MSP, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CFA65_HUMAN
Accessionⁱ	Q6ZU64Primary (citable) accession number: Q6ZU64 Secondary accession number(s): A2BDD8 Q8NDJ3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
	Last sequence update:	May 29, 2007
	Last modified:	December 2, 2020
	This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Reference proteome

Documents

Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families
Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q6ZU64 (CFA65_HUMAN)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Cilia- and flagella-associated protein 65

CFAP65

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Organism-specific databases

Miscellaneous databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Isoform 2 [Q6ZU64-3]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

Compositional bias

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ