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Protein

E3 ubiquitin-protein ligase UBR3

Gene

UBR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (By similarity). Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May play a role in Shh signaling by mediating the ubiquitination of Kif7 (By similarity). May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A (PubMed:27331610).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 189UBR-typePROSITE-ProRule annotationAdd BLAST72
Zinc fingeri1306 – 1364RING-type; degenerateAdd BLAST59

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UBR3 (EC:2.3.2.27)
Alternative name(s):
N-recognin-3
RING-type E3 ubiquitin transferase UBR3
Ubiquitin-protein ligase E3-alpha-3
Ubiquitin-protein ligase E3-alpha-III
Zinc finger protein 650
Gene namesi
Name:UBR3
Synonyms:KIAA2024, ZNF650
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000144357.16
HGNCiHGNC:30467 UBR3
MIMi613831 gene
neXtProtiNX_Q6ZT12

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei761 – 781HelicalSequence analysisAdd BLAST21
Transmembranei919 – 939HelicalSequence analysisAdd BLAST21
Transmembranei1806 – 1826HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi130507
OpenTargetsiENSG00000144357
PharmGKBiPA162407919

Polymorphism and mutation databases

BioMutaiUBR3
DMDMi166214992

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002781841 – 1888E3 ubiquitin-protein ligase UBR3Add BLAST1888

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineBy similarity1
Modified residuei344PhosphoserineBy similarity1
Modified residuei1199PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZT12
MaxQBiQ6ZT12
PaxDbiQ6ZT12
PeptideAtlasiQ6ZT12
PRIDEiQ6ZT12
ProteomicsDBi68252
68253 [Q6ZT12-2]
68254 [Q6ZT12-3]
68255 [Q6ZT12-4]

PTM databases

iPTMnetiQ6ZT12
PhosphoSitePlusiQ6ZT12

Expressioni

Gene expression databases

BgeeiENSG00000144357 Expressed in 209 organ(s), highest expression level in biceps brachii
CleanExiHS_UBR3
ExpressionAtlasiQ6ZT12 baseline and differential
GenevisibleiQ6ZT12 HS

Organism-specific databases

HPAiHPA035390

Interactioni

Subunit structurei

Interacts with UBE2A and UBE2B.By similarity

Protein-protein interaction databases

BioGridi126237, 50 interactors
IntActiQ6ZT12, 5 interactors
STRINGi9606.ENSP00000396068

Structurei

3D structure databases

ProteinModelPortaliQ6ZT12
SMRiQ6ZT12
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1168 – 1199Sequence analysisAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 118Ala-richAdd BLAST117
Compositional biasi1766 – 1808Cys-richAdd BLAST43

Sequence similaritiesi

Belongs to the UBR1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri118 – 189UBR-typePROSITE-ProRule annotationAdd BLAST72
Zinc fingeri1306 – 1364RING-type; degenerateAdd BLAST59

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1139 Eukaryota
ENOG410XRM3 LUCA
GeneTreeiENSGT00530000063055
HOVERGENiHBG108647
InParanoidiQ6ZT12
KOiK11978
OMAiKQKQFMQ
OrthoDBiEOG091G01MT
PhylomeDBiQ6ZT12
TreeFamiTF323875

Family and domain databases

InterProiView protein in InterPro
IPR039164 UBR1-like
IPR003126 Znf_UBR
PANTHERiPTHR21497 PTHR21497, 1 hit
PfamiView protein in Pfam
PF02207 zf-UBR, 1 hit
SMARTiView protein in SMART
SM00396 ZnF_UBR1, 1 hit
PROSITEiView protein in PROSITE
PS51157 ZF_UBR, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6ZT12-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAAAAAVG GQQPSQPELP APGLALDKAA TAAHLKAALS RPDNRAGAEE
60 70 80 90 100
LQALLERVLS AERPLAAAAG GEDAAAAGGG GGPGAAEEEA LEWCKCLLAG
110 120 130 140 150
GGGYDEFCAA VRAYDPAALC GLVWTANFVA YRCRTCGISP CMSLCAECFH
160 170 180 190 200
QGDHTGHDFN MFRSQAGGAC DCGDSNVMRE SGFCKRHQIK SSSNIPCVPK
210 220 230 240 250
DLLMMSEFVL PRFIFCLIQY LREGYNEPAA DGPSEKDLNK VLQLLEPQIS
260 270 280 290 300
FLEDLTKMGG AMRSVLTQVL TNQQNYKDLT SGLGENACVK KSHEKYLIAL
310 320 330 340 350
KSSGLTYPED KLVYGVQEPS AGTSSLAVQG FIGATGTLGQ VDSSDEDDQD
360 370 380 390 400
GSQGLGKRKR VKLSSGTKDQ SIMDVLKHKS FLEELLFWTI KYEFPQKMVT
410 420 430 440 450
FLLNMLPDQE YKVAFTKTFV QHYAFIMKTL KKSHESDTMS NRIVHISVQL
460 470 480 490 500
FSNEELARQV TEECQLLDIM VTVLLYMMES CLIKSELQDE ENSLHVVVNC
510 520 530 540 550
GEALLKNNTY WPLVSDFINI LSHQSVAKRF LEDHGLLVTW MNFVSFFQGM
560 570 580 590 600
NLNKRELNEH VEFESQTYYA AFAAELEACA QPMWGLLSHC KVRETQEYTR
610 620 630 640 650
NVVRYCLEAL QDWFDAINFV DEPAPNQVTF HLPLHRYYAM FLSKAVKCQE
660 670 680 690 700
LDLDSVLPDQ EMLMKLMIHP LQIQASLAEI HSNMWVRNGL QIKGQAMTYV
710 720 730 740 750
QSHFCNSMID PDIYLLQVCA SRLDPDYFIS SVFERFKVVD LLTMASQHQN
760 770 780 790 800
TVLDAEHERS MLEGALTFLV ILLSLRLHLG MSDDEILRAE MVAQLCMNDR
810 820 830 840 850
THSSLLDLIP ENPNPKSGII PGSYSFESVL SAVADFKAPV FEPGGSMQQG
860 870 880 890 900
MYTPKAEVWD QEFDPVMVIL RTVYRRDVQS AMDRYTAFLK QSGKFPGNPW
910 920 930 940 950
PPYKKRTSLH PSYKGLMRLL HCKTLHIVLF TLLYKILMDH QNLSEHVLCM
960 970 980 990 1000
VLYLIELGLE NSAEEESDEE ASVGGPERCH DSWFPGSNLV SNMRHFINYV
1010 1020 1030 1040 1050
RVRVPETAPE VKRDSPASTS SDNLGSLQNS GTAQVFSLVA ERRKKFQEII
1060 1070 1080 1090 1100
NRSSSEANQV VRPKTSSKWS APGSAPQLTT AILEIKESIL SLLIKLHHKL
1110 1120 1130 1140 1150
SGKQNSYYPP WLDDIEILIQ PEIPKYSHGD GITAVERILL KAASQSRMNK
1160 1170 1180 1190 1200
RIIEEICRKV TPPVPPKKVT AAEKKTLDKE ERRQKARERQ QKLLAEFASR
1210 1220 1230 1240 1250
QKSFMETAMD VDSPENDIPM EITTAEPQVS EAVYDCVICG QSGPSSEDRP
1260 1270 1280 1290 1300
TGLVVLLQAS SVLGQCRDNV EPKKLPISEE EQIYPWDTCA AVHDVRLSLL
1310 1320 1330 1340 1350
QRYFKDSSCL LAVSIGWEGG VYVQTCGHTL HIDCHKSYME SLRNDQVLQG
1360 1370 1380 1390 1400
FSVDKGEFTC PLCRQFANSV LPCYPGSNVE NNPWQRPSNK SIQDLIKEVE
1410 1420 1430 1440 1450
ELQGRPGAFP SETNLSKEME SVMKDIKNTT QKKYRDYSKT PGSPDNDFLF
1460 1470 1480 1490 1500
MYSVARTNLE LELIHRGGNL CSGGASTAGK RSCLNQLFHV LALHMRLYSI
1510 1520 1530 1540 1550
DSEYNPWRKL TQLEEMNPQL GYEEQQPEVP ILYHDVTSLL LIQILMMPQP
1560 1570 1580 1590 1600
LRKDHFTCIV KVLFTLLYTQ ALAALSVKCS EEDRSAWKHA GALKKSTCDA
1610 1620 1630 1640 1650
EKSYEVLLSF VISELFKGKL YHEEGTQECA MVNPIAWSPE SMEKCLQDFC
1660 1670 1680 1690 1700
LPFLRITSLL QHHLFGEDLP SCQEEEEFSV LASCLGLLPT FYQTEHPFIS
1710 1720 1730 1740 1750
ASCLDWPVPA FDIITQWCFE IKSFTERHAE QGKALLIQES KWKLPHLLQL
1760 1770 1780 1790 1800
PENYNTIFQY YHRKTCSVCT KVPKDPAVCL VCGTFVCLKG LCCKQQSYCE
1810 1820 1830 1840 1850
CVLHSQNCGA GTGIFLLINA SVIIIIRGHR FCLWGSVYLD AHGEEDRDLR
1860 1870 1880
RGKPLYICKE RYKVLEQQWI SHTFDHINKR WGPHYNGL
Length:1,888
Mass (Da):212,433
Last modified:January 15, 2008 - v2
Checksum:i5F5525B33D2E9153
GO
Isoform 2 (identifier: Q6ZT12-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1179: Missing.
     1180-1211: EERRQKARERQQKLLAEFASRQKSFMETAMDV → MIASKQRQFTEVFRIMSSTFSQSRFLYAAVVT

Show »
Length:709
Mass (Da):80,668
Checksum:i31BAF308F2AD6FB1
GO
Isoform 3 (identifier: Q6ZT12-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1494: Missing.

Show »
Length:394
Mass (Da):45,533
Checksum:i4B1B5048589F8CA6
GO
Isoform 4 (identifier: Q6ZT12-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1485-1485: N → NFCFIISPFTKSEIILCQYRKSHDKVYPKY

Note: No experimental confirmation available.
Show »
Length:1,917
Mass (Da):215,971
Checksum:iD4EC272A57F7B564
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y3K2H0Y3K2_HUMAN
E3 ubiquitin-protein ligase UBR3
UBR3
950Annotation score:
H7C481H7C481_HUMAN
E3 ubiquitin-protein ligase UBR3
UBR3
384Annotation score:
H7BZD8H7BZD8_HUMAN
E3 ubiquitin-protein ligase UBR3
UBR3
588Annotation score:
F8WEB7F8WEB7_HUMAN
E3 ubiquitin-protein ligase UBR3
UBR3
77Annotation score:

Sequence cautioni

The sequence BAC23120 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC86783 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC87032 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAG64179 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAD38857 differs from that shown. Reason: Erroneous termination at position 1525. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1791L → F in BAC87032 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0231411 – 1494Missing in isoform 3. 1 PublicationAdd BLAST1494
Alternative sequenceiVSP_0303541 – 1179Missing in isoform 2. 1 PublicationAdd BLAST1179
Alternative sequenceiVSP_0231421180 – 1211EERRQ…TAMDV → MIASKQRQFTEVFRIMSSTF SQSRFLYAAVVT in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_0364051485N → NFCFIISPFTKSEIILCQYR KSHDKVYPKY in isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095944 mRNA Translation: BAC23120.1 Different initiation.
AK126998 mRNA Translation: BAC86783.1 Different initiation.
AK127553 mRNA Translation: BAC87032.1 Different initiation.
AK128490 mRNA Translation: BAC87462.1
AK303062 mRNA Translation: BAG64179.1 Different initiation.
AC009967 Genomic DNA No translation available.
AC079151 Genomic DNA No translation available.
AC092641 Genomic DNA Translation: AAY14890.1
AL834144 mRNA Translation: CAD38857.1 Sequence problems.
CCDSiCCDS2238.2 [Q6ZT12-1]
RefSeqiNP_742067.3, NM_172070.3 [Q6ZT12-1]
UniGeneiHs.379548

Genome annotation databases

EnsembliENST00000272793; ENSP00000272793; ENSG00000144357 [Q6ZT12-1]
ENST00000418381; ENSP00000396068; ENSG00000144357 [Q6ZT12-1]
GeneIDi130507
KEGGihsa:130507
UCSCiuc010zdi.3 human [Q6ZT12-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095944 mRNA Translation: BAC23120.1 Different initiation.
AK126998 mRNA Translation: BAC86783.1 Different initiation.
AK127553 mRNA Translation: BAC87032.1 Different initiation.
AK128490 mRNA Translation: BAC87462.1
AK303062 mRNA Translation: BAG64179.1 Different initiation.
AC009967 Genomic DNA No translation available.
AC079151 Genomic DNA No translation available.
AC092641 Genomic DNA Translation: AAY14890.1
AL834144 mRNA Translation: CAD38857.1 Sequence problems.
CCDSiCCDS2238.2 [Q6ZT12-1]
RefSeqiNP_742067.3, NM_172070.3 [Q6ZT12-1]
UniGeneiHs.379548

3D structure databases

ProteinModelPortaliQ6ZT12
SMRiQ6ZT12
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126237, 50 interactors
IntActiQ6ZT12, 5 interactors
STRINGi9606.ENSP00000396068

PTM databases

iPTMnetiQ6ZT12
PhosphoSitePlusiQ6ZT12

Polymorphism and mutation databases

BioMutaiUBR3
DMDMi166214992

Proteomic databases

EPDiQ6ZT12
MaxQBiQ6ZT12
PaxDbiQ6ZT12
PeptideAtlasiQ6ZT12
PRIDEiQ6ZT12
ProteomicsDBi68252
68253 [Q6ZT12-2]
68254 [Q6ZT12-3]
68255 [Q6ZT12-4]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272793; ENSP00000272793; ENSG00000144357 [Q6ZT12-1]
ENST00000418381; ENSP00000396068; ENSG00000144357 [Q6ZT12-1]
GeneIDi130507
KEGGihsa:130507
UCSCiuc010zdi.3 human [Q6ZT12-1]

Organism-specific databases

CTDi130507
DisGeNETi130507
EuPathDBiHostDB:ENSG00000144357.16
GeneCardsiUBR3
HGNCiHGNC:30467 UBR3
HPAiHPA035390
MIMi613831 gene
neXtProtiNX_Q6ZT12
OpenTargetsiENSG00000144357
PharmGKBiPA162407919
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1139 Eukaryota
ENOG410XRM3 LUCA
GeneTreeiENSGT00530000063055
HOVERGENiHBG108647
InParanoidiQ6ZT12
KOiK11978
OMAiKQKQFMQ
OrthoDBiEOG091G01MT
PhylomeDBiQ6ZT12
TreeFamiTF323875

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

ChiTaRSiUBR3 human
GenomeRNAii130507
PROiPR:Q6ZT12
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144357 Expressed in 209 organ(s), highest expression level in biceps brachii
CleanExiHS_UBR3
ExpressionAtlasiQ6ZT12 baseline and differential
GenevisibleiQ6ZT12 HS

Family and domain databases

InterProiView protein in InterPro
IPR039164 UBR1-like
IPR003126 Znf_UBR
PANTHERiPTHR21497 PTHR21497, 1 hit
PfamiView protein in Pfam
PF02207 zf-UBR, 1 hit
SMARTiView protein in SMART
SM00396 ZnF_UBR1, 1 hit
PROSITEiView protein in PROSITE
PS51157 ZF_UBR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBR3_HUMAN
AccessioniPrimary (citable) accession number: Q6ZT12
Secondary accession number(s): B4DZR7
, Q2KHN5, Q6ZR55, Q6ZSC2, Q8IVE7, Q8ND96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: January 15, 2008
Last modified: October 10, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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