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Protein

DNA topoisomerase 2-binding protein 1

Gene

Topbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for DNA replication (By similarity). Plays a role in the rescue of stalled replication forks and checkpoint control (PubMed:14718568). Binds double-stranded DNA breaks and nicks as well as single-stranded DNA (By similarity). Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage (By similarity). Induces a large increase in the kinase activity of ATR (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2-binding protein 1
Alternative name(s):
DNA topoisomerase II-beta-binding protein 1
Short name:
TopBP1
DNA topoisomerase II-binding protein 1
Gene namesi
Name:Topbp1
Synonyms:Kiaa0259
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1920018 Topbp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726321 – 1515DNA topoisomerase 2-binding protein 1Add BLAST1515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei298PhosphothreonineBy similarity1
Modified residuei782PhosphothreonineBy similarity1
Modified residuei851PhosphothreonineBy similarity1
Modified residuei862PhosphoserineCombined sources1
Modified residuei863PhosphoserineCombined sources1
Modified residuei866PhosphoserineBy similarity1
Modified residuei888PhosphoserineBy similarity1
Modified residuei890PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to X-ray irradiation.By similarity
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6ZQF0
MaxQBiQ6ZQF0
PaxDbiQ6ZQF0
PeptideAtlasiQ6ZQF0
PRIDEiQ6ZQF0

PTM databases

iPTMnetiQ6ZQF0
PhosphoSitePlusiQ6ZQF0

Expressioni

Tissue specificityi

Highly expressed in testis.1 Publication

Developmental stagei

Levels increase during the first 3 weeks after birth and remain high in the fourth week.1 Publication

Gene expression databases

BgeeiENSMUSG00000032555 Expressed in 285 organ(s), highest expression level in cochlea
CleanExiMM_TOPBP1
ExpressionAtlasiQ6ZQF0 baseline and differential
GenevisibleiQ6ZQF0 MM

Interactioni

Subunit structurei

Interacts with POLE. Interacts with RAD9A. Interacts with UBR5. Interacts with E2F1. Interacts with PML. Interacts with SMARCA2. Interacts with SMARCA4. Interacts with RHNO1. May interact with TOP2B. Interacts with TICRR. Interacts with HELB.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231681, 9 interactors
IntActiQ6ZQF0, 10 interactors
MINTiQ6ZQF0
STRINGi10090.ENSMUSP00000035164

Structurei

Secondary structure

11515
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ6ZQF0
SMRiQ6ZQF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 189BRCT 1PROSITE-ProRule annotationAdd BLAST89
Domaini195 – 284BRCT 2PROSITE-ProRule annotationAdd BLAST90
Domaini353 – 443BRCT 3PROSITE-ProRule annotationAdd BLAST91
Domaini551 – 636BRCT 4PROSITE-ProRule annotationAdd BLAST86
Domaini644 – 741BRCT 5PROSITE-ProRule annotationAdd BLAST98
Domaini902 – 993BRCT 6PROSITE-ProRule annotationAdd BLAST92
Domaini1255 – 1347BRCT 7PROSITE-ProRule annotationAdd BLAST93

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1510 – 1513Nuclear localization signalSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1207 – 1246Pro-richAdd BLAST40

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1929 Eukaryota
ENOG410XPFH LUCA
GeneTreeiENSGT00840000129846
HOVERGENiHBG067053
InParanoidiQ6ZQF0
KOiK10728
OMAiCAQEYKH
OrthoDBiEOG091G02MY
PhylomeDBiQ6ZQF0
TreeFamiTF326403

Family and domain databases

CDDicd00027 BRCT, 6 hits
Gene3Di3.40.50.10190, 9 hits
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
PfamiView protein in Pfam
PF00533 BRCT, 4 hits
PF12738 PTCB-BRCT, 2 hits
SMARTiView protein in SMART
SM00292 BRCT, 8 hits
SUPFAMiSSF52113 SSF52113, 7 hits
PROSITEiView protein in PROSITE
PS50172 BRCT, 6 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q6ZQF0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRNDQEPFL VKFLKSSDNS ECFFKALESI KELQSEDYLQ IITDEEALKI
60 70 80 90 100
RENDKSLYIC DRFSGTVFDH LKQLGCRIVG PQVVTFCMRH QQCVPRAEHP
110 120 130 140 150
VYNMIMSDVT VSCTSLDKDK REEVHKYVQM MGGRVYRDLN VSVTHLIAGE
160 170 180 190 200
VGSKKYLVAA NLKKPILLPS WIKTLWEKSQ EKKITKYTDV NMEDFKCPIF
210 220 230 240 250
LGCIICVTGL NGIHRKTVQQ LTAKHGGQYM GQLKMNECTH LIVQEPKGQK
260 270 280 290 300
YECARRWNVH CVTLQWFHDS IEKGFCQDES IYKAETRVEA KMVPDTSTPT
310 320 330 340 350
AQSNAESHTL ADVSHISNIN GSCVNETMFG STTSKLECSL ENLENLDISM
360 370 380 390 400
FQAPEDLLDG CRIYLCGFSG RKLDKLRRLI NSGGGVRFNQ LNEDVTHVIV
410 420 430 440 450
GDYDDDVRQF WSKSSHRPHV VGAKWLLECF TKGYILPEES YIHTNYQPAG
460 470 480 490 500
IAVSDQPGNQ TAVLDKSGSF SKSALVPAER LQQADEDLLA QYGNDDSTMV
510 520 530 540 550
EAKLSEALEP EVGPCPGSAH REPCDDSTHI SVQEENKSSV SHCILDDSTV
560 570 580 590 600
REEGLFSQKS FLVLGFSVEN KCNIVDIIRE HAGKIVSLPS RIVADYAVVP
610 620 630 640 650
LLGCEVDVTV GEVVTNTWLV TCIDNQTLVD PKSNPLFTPV SVMSGVTPLE
660 670 680 690 700
DCVISFSQCV GAERDSLVFL ANHLGASVQE FFVRKANAKK GMLASTHLIV
710 720 730 740 750
KEPTGSKYEA AKKWSLPAVN ISWLLETARI GKRADENHFL VDNAPKQEQV
760 770 780 790 800
LETKIPNGVS SNPDLPAHPD AHLEIHRKKA VTPLDMNRFQ SRAFRAVISQ
810 820 830 840 850
QRGQDPTFPP VRQPLTKEPS LHLDTPSKFL SKDKLFKPSF DVTDALAALE
860 870 880 890 900
TPNAASQKRK LSSPLSEVIV RNLTVALANS SRNTDSHSAS PQLKGAHLEE
910 920 930 940 950
EETRKPLDSV VVCVSKKLSK KQSELNGVAA SLGAEYRWSF DETVTHFIYQ
960 970 980 990 1000
GRANDSNREY KSAKERGVHI VSEHWLLECA QEYKHLPESL YPHTYNPKMS
1010 1020 1030 1040 1050
LDINTVQDGR LCNSRAPLAV SASKDDGPDH LSVEGNETNT MGTNDKESPL
1060 1070 1080 1090 1100
LNGSGRDDCK GALTQALEMR ENFQKQLQEI MSATCIVKTP AQKTCMSRSS
1110 1120 1130 1140 1150
CNSASSTPDS ARSVRSGRSR VLEALRQSRQ AVPDVNTEPS QNEQIIWDDP
1160 1170 1180 1190 1200
TAREERARLA SNLQWPSDPT QHSELQVEIK MPDDSPSRKP VYHSEIAEQA
1210 1220 1230 1240 1250
SCVTQAPGHP GSEEPEPPVA ERPLIPEPQA PAVASPLAKP PVAPQPADKI
1260 1270 1280 1290 1300
ETQEETHRKV KKQYVFQMSS LNSQERIDYC RLIKDLGGSV IEKQCSDPSC
1310 1320 1330 1340 1350
THMVVGYPLR NEKYLASMAA GKWVLHRSYL DACKTAGRFV QEEDYEWGSS
1360 1370 1380 1390 1400
SILDALPDVT EHQQKLALAA MRWRKRIQQS QESGIVEGAF SGWKAILRVD
1410 1420 1430 1440 1450
RPREAGFKRL LQAGGAKVLS GHPEPLLKDA THLFCDFNKL KPDDCRVFIA
1460 1470 1480 1490 1500
EATAQNMVCL KTEYIADYLM LESPPCADNY RVSEAALFHN KKGGPGLPQK
1510
RKTPAENVVK RPRVH
Length:1,515
Mass (Da):168,860
Last modified:April 26, 2005 - v2
Checksum:iE2E729D2E57B6F2F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WS55A0A087WS55_MOUSE
DNA topoisomerase 2-binding protein...
Topbp1
257Annotation score:
A0A087WPG9A0A087WPG9_MOUSE
DNA topoisomerase 2-binding protein...
Topbp1
130Annotation score:
A0A087WS20A0A087WS20_MOUSE
DNA topoisomerase 2-binding protein...
Topbp1
66Annotation score:

Sequence cautioni

The sequence AAH07170 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC39345 differs from that shown. Reason: Frameshift at position 183.Curated
The sequence BAC97914 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti324V → I in AAH07170 (PubMed:15489334).Curated1
Sequence conflicti461T → I in AAH07170 (PubMed:15489334).Curated1
Sequence conflicti521R → L in AAH07170 (PubMed:15489334).Curated1
Sequence conflicti607D → E in AAH07170 (PubMed:15489334).Curated1
Sequence conflicti835L → P in AAH62111 (PubMed:15489334).Curated1
Sequence conflicti1041M → I in AAH07170 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1420S → P in strain: FVB/N. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129104 mRNA Translation: BAC97914.1 Different initiation.
BC006707 mRNA Translation: AAH06707.1
BC007170 mRNA Translation: AAH07170.1 Different initiation.
BC026608 mRNA Translation: AAH26608.1
BC049797 mRNA Translation: AAH49797.1
BC062111 mRNA Translation: AAH62111.1
AK084654 mRNA Translation: BAC39241.2
AK085031 mRNA Translation: BAC39345.1 Frameshift.
CCDSiCCDS23453.1
RefSeqiNP_795953.2, NM_176979.5
XP_006511770.1, XM_006511707.3
UniGeneiMm.259893

Genome annotation databases

EnsembliENSMUST00000035164; ENSMUSP00000035164; ENSMUSG00000032555
GeneIDi235559
KEGGimmu:235559
UCSCiuc009rgl.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129104 mRNA Translation: BAC97914.1 Different initiation.
BC006707 mRNA Translation: AAH06707.1
BC007170 mRNA Translation: AAH07170.1 Different initiation.
BC026608 mRNA Translation: AAH26608.1
BC049797 mRNA Translation: AAH49797.1
BC062111 mRNA Translation: AAH62111.1
AK084654 mRNA Translation: BAC39241.2
AK085031 mRNA Translation: BAC39345.1 Frameshift.
CCDSiCCDS23453.1
RefSeqiNP_795953.2, NM_176979.5
XP_006511770.1, XM_006511707.3
UniGeneiMm.259893

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5U6KX-ray2.60A/B/C/D/E/F/G/H553-746[»]
ProteinModelPortaliQ6ZQF0
SMRiQ6ZQF0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231681, 9 interactors
IntActiQ6ZQF0, 10 interactors
MINTiQ6ZQF0
STRINGi10090.ENSMUSP00000035164

PTM databases

iPTMnetiQ6ZQF0
PhosphoSitePlusiQ6ZQF0

Proteomic databases

EPDiQ6ZQF0
MaxQBiQ6ZQF0
PaxDbiQ6ZQF0
PeptideAtlasiQ6ZQF0
PRIDEiQ6ZQF0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035164; ENSMUSP00000035164; ENSMUSG00000032555
GeneIDi235559
KEGGimmu:235559
UCSCiuc009rgl.1 mouse

Organism-specific databases

CTDi11073
MGIiMGI:1920018 Topbp1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1929 Eukaryota
ENOG410XPFH LUCA
GeneTreeiENSGT00840000129846
HOVERGENiHBG067053
InParanoidiQ6ZQF0
KOiK10728
OMAiCAQEYKH
OrthoDBiEOG091G02MY
PhylomeDBiQ6ZQF0
TreeFamiTF326403

Enzyme and pathway databases

ReactomeiR-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Miscellaneous databases

PROiPR:Q6ZQF0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032555 Expressed in 285 organ(s), highest expression level in cochlea
CleanExiMM_TOPBP1
ExpressionAtlasiQ6ZQF0 baseline and differential
GenevisibleiQ6ZQF0 MM

Family and domain databases

CDDicd00027 BRCT, 6 hits
Gene3Di3.40.50.10190, 9 hits
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
PfamiView protein in Pfam
PF00533 BRCT, 4 hits
PF12738 PTCB-BRCT, 2 hits
SMARTiView protein in SMART
SM00292 BRCT, 8 hits
SUPFAMiSSF52113 SSF52113, 7 hits
PROSITEiView protein in PROSITE
PS50172 BRCT, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTOPB1_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQF0
Secondary accession number(s): Q6P6P0
, Q80Y33, Q8BUI0, Q8BUK1, Q8R348, Q91VX3, Q922X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: September 12, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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