UniProtKB - Q6ZQB6 (VIP2_MOUSE)
Protein
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2
Gene
Ppip5k2
Organism
Mus musculus (Mouse)
Status
Functioni
Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4 (PubMed:17690096). PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation (PubMed:17690096). Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4 (PubMed:17690096). Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 (PubMed:17690096). Required for normal hearing (PubMed:29590114).2 Publications
Catalytic activityi
- 1D-myo-inositol hexakisphosphate + ATP = 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ADP1 PublicationEC:2.7.4.211 Publication
- 1-diphospho-myo-inositol 2,3,4,5,6-pentakisphosphate + ATP + H+ = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP1 PublicationEC:2.7.4.211 Publication
- 1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP1 PublicationEC:2.7.4.241 Publication
- 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H+ = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP1 PublicationEC:2.7.4.241 Publication
Kineticsi
- KM=0.23 µM for InsP61 Publication
- KM=0.54 µM for InsP71 Publication
- Vmax=1.70 nmol/min/mg enzyme with InsP6 as substrate1 Publication
- Vmax=5.23 nmol/min/mg enzyme with InsP7 as substrate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 140 | ATPBy similarity | 1 | |
Binding sitei | 193 | ATPBy similarity | 1 | |
Binding sitei | 200 | ATPBy similarity | 1 | |
Binding sitei | 219 | ATPBy similarity | 1 | |
Binding sitei | 254 | SubstrateBy similarity | 1 | |
Binding sitei | 268 | SubstrateBy similarity | 1 | |
Binding sitei | 270 | ATPBy similarity | 1 | |
Binding sitei | 315 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 243 – 246 | ATPBy similarity | 4 | |
Nucleotide bindingi | 252 – 254 | ATPBy similarity | 3 | |
Nucleotide bindingi | 327 – 329 | ATPBy similarity | 3 |
GO - Molecular functioni
- 5-diphosphoinositol pentakisphosphate 3-kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB
- diphosphoinositol-pentakisphosphate kinase activity Source: MGI
- inositol-1,3,4,5,6-pentakisphosphate kinase activity Source: UniProtKB
- inositol heptakisphosphate kinase activity Source: GO_Central
- inositol hexakisphosphate 1-kinase activity Source: UniProtKB-EC
- inositol hexakisphosphate 3-kinase activity Source: UniProtKB-EC
- inositol hexakisphosphate 5-kinase activity Source: UniProtKB
- inositol hexakisphosphate kinase activity Source: MGI
GO - Biological processi
- inositol metabolic process Source: UniProtKB
- inositol phosphate biosynthetic process Source: GO_Central
- sensory perception of sound Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Hearing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1855167, Synthesis of pyrophosphates in the cytosol |
SABIO-RKi | Q6ZQB6 |
Names & Taxonomyi
Protein namesi | Recommended name: Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 (EC:2.7.4.211 Publication, EC:2.7.4.241 Publication)Alternative name(s): Diphosphoinositol pentakisphosphate kinase 2 Histidine acid phosphatase domain-containing protein 1 InsP6 and PP-IP5 kinase 2 VIP1 homolog 2 Short name: mmVIP2 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2142810, Ppip5k2 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315693 | 1 – 1129 | Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2Add BLAST | 1129 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 44 | PhosphoserineCombined sources | 1 | |
Modified residuei | 229 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1051 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1058 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1066 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1106 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1107 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q6ZQB6 |
PaxDbi | Q6ZQB6 |
PeptideAtlasi | Q6ZQB6 |
PRIDEi | Q6ZQB6 |
PTM databases
iPTMneti | Q6ZQB6 |
PhosphoSitePlusi | Q6ZQB6 |
Expressioni
Tissue specificityi
Ubiquitously expressed (PubMed:29590114). Expressed in the cochlear and vestibular sensory hair cells, supporting cells and spiral ganglion neurons (PubMed:29590114).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040648, Expressed in pes and 273 other tissues |
Genevisiblei | Q6ZQB6, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000108466 |
Miscellaneous databases
RNActi | Q6ZQB6, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 59 – 60 | Substrate bindingBy similarity | 2 | |
Regioni | 219 – 220 | Substrate bindingBy similarity | 2 | |
Regioni | 332 – 335 | Substrate bindingBy similarity | 4 | |
Regioni | 377 – 448 | Polyphosphoinositide-binding domainBy similarityAdd BLAST | 72 |
Domaini
The C-terminal acid phosphatase-like domain binds PtdIns(3,4,5)P3 and InsP6. Despite its similarity with the phosphatase domain of histidine acid phosphatases, it has no phosphatase activity.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1057, Eukaryota |
GeneTreei | ENSGT00390000009048 |
HOGENOMi | CLU_000914_0_0_1 |
InParanoidi | Q6ZQB6 |
OMAi | PWLFRER |
OrthoDBi | 93740at2759 |
Family and domain databases
CDDi | cd07061, HP_HAP_like, 1 hit |
InterProi | View protein in InterPro IPR033379, Acid_Pase_AS IPR000560, His_Pase_clade-2 IPR037446, His_Pase_VIP1 IPR029033, His_PPase_superfam IPR040557, VIP1_N |
PANTHERi | PTHR12750, PTHR12750, 2 hits |
Pfami | View protein in Pfam PF00328, His_Phos_2, 1 hit PF18086, PPIP5K2_N, 1 hit |
SUPFAMi | SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00616, HIS_ACID_PHOSPHAT_1, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6ZQB6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSNSRKMSEP PRFFVGPEDA EINPGNYRRF FHHAEEEEEE EDESPPERQI
60 70 80 90 100
VVGICSMAKK SKSKPMKEIL ERISLFKYIT VVVFEEEIIL NEPVENWPLC
110 120 130 140 150
DCLISFHSKG FPLDKAVAYA KLRNPFVIND LNMQYLIQDR RDVYSILQAE
160 170 180 190 200
GILLPRYAIL NRDPNNPKEC NLIEGEDHVE VNGEVFQKPF VEKPVSAEDH
210 220 230 240 250
NVYIYYPTSA GGGSQRLFRK IGSRSSVYSP ESNVRKTGSY IYEEFMPTDG
260 270 280 290 300
TDVKVYTVGP DYAHAEARKS PALDGKVERD SEGKEVRYPV ILNAREKLIA
310 320 330 340 350
WKVCLAFKQT VCGFDLLRAN GQSYVCDVNG FSFVKNSMKY YDDCAKILGN
360 370 380 390 400
IVMRELAPQF HIPWSIPLEA EDIPIVPTTS GTMMELRCVI AVIRHGDRTP
410 420 430 440 450
KQKMKMEVRH QKFFDLFEKC DGYKSGKLKL KKPKQLQEVL DIARQLLMEL
460 470 480 490 500
GQNNDSEIEE NKSKLEQLKT VLEMYGHFSG INRKVQLTYL PHGCPKTSSE
510 520 530 540 550
EEDNRREEPS LLLVLKWGGE LTPAGRVQAE ELGRAFRCMY PGGQGDYAGF
560 570 580 590 600
PGCGLLRLHS TYRHDLKIYA SDEGRVQMTA AAFAKGLLAL EGELTPILVQ
610 620 630 640 650
MVKSANMNGL LDSDSDSLSS CQQRVKARLH EILQKDRDFT AEDYEKLTPS
660 670 680 690 700
GSISVIKSMH LIKNPVKTCD KVYSLIQSLT SQIRYRMEDP KSADIQLYHS
710 720 730 740 750
ETLELMLRRW SKLEKDFKTK NGRYDISKIP DIYDCIKYDV QHNGSLKLEN
760 770 780 790 800
TMELYRLSKA LADIVIPQEY GITKAEKLEI AKGYCTPLVR KIRSDLQRTQ
810 820 830 840 850
DDDTVNKLHP VYSRGVLSPE RHVRTRLYFT SESHVHSLLS ILRYGALCDD
860 870 880 890 900
SKDEQWKRAM DYLNVVNELN YMTQIVIMLY EDPNKDLSSE ERFHVELHFS
910 920 930 940 950
PGAKGCEEDK NLPSGYGYRP ASRENEGRRS LKTDDDEPHT SKRDEVDRAV
960 970 980 990 1000
MLFKPLVSEP IHIHRKSPLP RSRKITANEV VSENANYLRT PRNLVEQKQN
1010 1020 1030 1040 1050
PTVGFELYSM VPSICPLETL HNALFLKQVD DFLASIASPS TEVLRKVPEM
1060 1070 1080 1090 1100
SSMATRSSPG MRRKISLNTY TPTKILPTPP AALKSSKASS KAAAGGPSQA
1110 1120
MAPHTSSRKK SINSKTEGHE PKKSTGKKR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q9J4 | E9Q9J4_MOUSE | Inositol hexakisphosphate and dipho... | Ppip5k2 | 1,242 | Annotation score: | ||
A0A087WPZ7 | A0A087WPZ7_MOUSE | Inositol hexakisphosphate and dipho... | Ppip5k2 | 92 | Annotation score: |
Sequence cautioni
The sequence BAC97950 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 197 | A → V in BAE38567 (PubMed:14621295).Curated | 1 | |
Sequence conflicti | 582 | A → T in AAH53396 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 729 | I → V in AAH53396 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1035 | S → P in AAH53396 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1109 | K → M in BAC97950 (PubMed:14621295).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041618 | 1 – 6 | Missing in isoform 3. 1 Publication | 6 | |
Alternative sequenceiVSP_030637 | 545 | Missing in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_030638 | 1051 – 1129 | SSMAT…TGKKR → CMEFTFIVT in isoform 2. 1 PublicationAdd BLAST | 79 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129140 mRNA Translation: BAC97950.1 Different initiation. AK138622 mRNA Translation: BAE23724.1 AK166095 mRNA Translation: BAE38567.1 AC099860 Genomic DNA No translation available. AC162298 Genomic DNA No translation available. BC053396 mRNA Translation: AAH53396.1 |
CCDSi | CCDS35678.2 [Q6ZQB6-1] |
RefSeqi | NP_776121.4, NM_173760.5 [Q6ZQB6-1] |
Genome annotation databases
Ensembli | ENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648 [Q6ZQB6-1] ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648 [Q6ZQB6-3] |
GeneIDi | 227399 |
KEGGi | mmu:227399 |
UCSCi | uc007cfk.4, mouse [Q6ZQB6-1] uc007cfl.1, mouse [Q6ZQB6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129140 mRNA Translation: BAC97950.1 Different initiation. AK138622 mRNA Translation: BAE23724.1 AK166095 mRNA Translation: BAE38567.1 AC099860 Genomic DNA No translation available. AC162298 Genomic DNA No translation available. BC053396 mRNA Translation: AAH53396.1 |
CCDSi | CCDS35678.2 [Q6ZQB6-1] |
RefSeqi | NP_776121.4, NM_173760.5 [Q6ZQB6-1] |
3D structure databases
SMRi | Q6ZQB6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000108466 |
PTM databases
iPTMneti | Q6ZQB6 |
PhosphoSitePlusi | Q6ZQB6 |
Proteomic databases
jPOSTi | Q6ZQB6 |
PaxDbi | Q6ZQB6 |
PeptideAtlasi | Q6ZQB6 |
PRIDEi | Q6ZQB6 |
Protocols and materials databases
Antibodypediai | 44750, 69 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648 [Q6ZQB6-1] ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648 [Q6ZQB6-3] |
GeneIDi | 227399 |
KEGGi | mmu:227399 |
UCSCi | uc007cfk.4, mouse [Q6ZQB6-1] uc007cfl.1, mouse [Q6ZQB6-2] |
Organism-specific databases
CTDi | 23262 |
MGIi | MGI:2142810, Ppip5k2 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1057, Eukaryota |
GeneTreei | ENSGT00390000009048 |
HOGENOMi | CLU_000914_0_0_1 |
InParanoidi | Q6ZQB6 |
OMAi | PWLFRER |
OrthoDBi | 93740at2759 |
Enzyme and pathway databases
Reactomei | R-MMU-1855167, Synthesis of pyrophosphates in the cytosol |
SABIO-RKi | Q6ZQB6 |
Miscellaneous databases
BioGRID-ORCSi | 227399, 2 hits in 12 CRISPR screens |
PROi | PR:Q6ZQB6 |
RNActi | Q6ZQB6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040648, Expressed in pes and 273 other tissues |
Genevisiblei | Q6ZQB6, MM |
Family and domain databases
CDDi | cd07061, HP_HAP_like, 1 hit |
InterProi | View protein in InterPro IPR033379, Acid_Pase_AS IPR000560, His_Pase_clade-2 IPR037446, His_Pase_VIP1 IPR029033, His_PPase_superfam IPR040557, VIP1_N |
PANTHERi | PTHR12750, PTHR12750, 2 hits |
Pfami | View protein in Pfam PF00328, His_Phos_2, 1 hit PF18086, PPIP5K2_N, 1 hit |
SUPFAMi | SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00616, HIS_ACID_PHOSPHAT_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VIP2_MOUSE | |
Accessioni | Q6ZQB6Primary (citable) accession number: Q6ZQB6 Secondary accession number(s): E9PVE9 Q7TPU4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 116 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families