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Protein

Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2

Gene

Ppip5k2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate = ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol hexakisphosphate = ADP + 1D-myo-inositol 1-diphosphate 2,3,4,5,6-pentakisphosphate.1 Publication

Kineticsi

  1. KM=0.23 µM for InsP61 Publication
  2. KM=0.54 µM for InsP71 Publication
  1. Vmax=1.70 nmol/min/mg enzyme with InsP6 as substrate1 Publication
  2. Vmax=5.23 nmol/min/mg enzyme with InsP7 as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140ATPBy similarity1
Binding sitei193ATPBy similarity1
Binding sitei200ATPBy similarity1
Binding sitei219ATPBy similarity1
Binding sitei254SubstrateBy similarity1
Binding sitei268SubstrateBy similarity1
Binding sitei270ATPBy similarity1
Binding sitei315ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi243 – 246ATPBy similarity4
Nucleotide bindingi252 – 254ATPBy similarity3
Nucleotide bindingi327 – 329ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1855167 Synthesis of pyrophosphates in the cytosol
SABIO-RKiQ6ZQB6

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 (EC:2.7.4.211 Publication, EC:2.7.4.241 Publication)
Alternative name(s):
Diphosphoinositol pentakisphosphate kinase 2
Histidine acid phosphatase domain-containing protein 1
InsP6 and PP-IP5 kinase 2
VIP1 homolog 2
Short name:
mmVIP2
Gene namesi
Name:Ppip5k2
Synonyms:Hisppd1, Kiaa0433, Vip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2142810 Ppip5k2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003156931 – 1129Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2Add BLAST1129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44PhosphoserineCombined sources1
Modified residuei229PhosphoserineBy similarity1
Modified residuei1051PhosphoserineBy similarity1
Modified residuei1058PhosphoserineBy similarity1
Modified residuei1066PhosphoserineCombined sources1
Modified residuei1106PhosphoserineBy similarity1
Modified residuei1107PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6ZQB6
PeptideAtlasiQ6ZQB6
PRIDEiQ6ZQB6

PTM databases

iPTMnetiQ6ZQB6
PhosphoSitePlusiQ6ZQB6

Expressioni

Gene expression databases

BgeeiENSMUSG00000040648 Expressed in 257 organ(s), highest expression level in pes
CleanExiMM_HISPPD1
ExpressionAtlasiQ6ZQB6 baseline and differential
GenevisibleiQ6ZQB6 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043401

Structurei

3D structure databases

ProteinModelPortaliQ6ZQB6
SMRiQ6ZQB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 60Substrate bindingBy similarity2
Regioni219 – 220Substrate bindingBy similarity2
Regioni332 – 335Substrate bindingBy similarity4
Regioni377 – 448Polyphosphoinositide-binding domainBy similarityAdd BLAST72

Domaini

The C-terminal acid phosphatase-like domain binds PtdIns(3,4,5)P3 and InsP6. Despite its similarity with the phosphatase domain of histidine acid phosphatases, it has no phosphatase activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1057 Eukaryota
ENOG410XNSN LUCA
GeneTreeiENSGT00390000009048
HOGENOMiHOG000177917
HOVERGENiHBG108657
InParanoidiQ6ZQB6
KOiK13024
OMAiPWLFRER

Family and domain databases

CDDicd07061 HP_HAP_like, 1 hit
InterProiView protein in InterPro
IPR033379 Acid_Pase_AS
IPR000560 His_Pase_clade-2
IPR037446 His_Pase_VIP1
IPR029033 His_PPase_superfam
PANTHERiPTHR12750 PTHR12750, 2 hits
PfamiView protein in Pfam
PF00328 His_Phos_2, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00616 HIS_ACID_PHOSPHAT_1, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6ZQB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSNSRKMSEP PRFFVGPEDA EINPGNYRRF FHHAEEEEEE EDESPPERQI
60 70 80 90 100
VVGICSMAKK SKSKPMKEIL ERISLFKYIT VVVFEEEIIL NEPVENWPLC
110 120 130 140 150
DCLISFHSKG FPLDKAVAYA KLRNPFVIND LNMQYLIQDR RDVYSILQAE
160 170 180 190 200
GILLPRYAIL NRDPNNPKEC NLIEGEDHVE VNGEVFQKPF VEKPVSAEDH
210 220 230 240 250
NVYIYYPTSA GGGSQRLFRK IGSRSSVYSP ESNVRKTGSY IYEEFMPTDG
260 270 280 290 300
TDVKVYTVGP DYAHAEARKS PALDGKVERD SEGKEVRYPV ILNAREKLIA
310 320 330 340 350
WKVCLAFKQT VCGFDLLRAN GQSYVCDVNG FSFVKNSMKY YDDCAKILGN
360 370 380 390 400
IVMRELAPQF HIPWSIPLEA EDIPIVPTTS GTMMELRCVI AVIRHGDRTP
410 420 430 440 450
KQKMKMEVRH QKFFDLFEKC DGYKSGKLKL KKPKQLQEVL DIARQLLMEL
460 470 480 490 500
GQNNDSEIEE NKSKLEQLKT VLEMYGHFSG INRKVQLTYL PHGCPKTSSE
510 520 530 540 550
EEDNRREEPS LLLVLKWGGE LTPAGRVQAE ELGRAFRCMY PGGQGDYAGF
560 570 580 590 600
PGCGLLRLHS TYRHDLKIYA SDEGRVQMTA AAFAKGLLAL EGELTPILVQ
610 620 630 640 650
MVKSANMNGL LDSDSDSLSS CQQRVKARLH EILQKDRDFT AEDYEKLTPS
660 670 680 690 700
GSISVIKSMH LIKNPVKTCD KVYSLIQSLT SQIRYRMEDP KSADIQLYHS
710 720 730 740 750
ETLELMLRRW SKLEKDFKTK NGRYDISKIP DIYDCIKYDV QHNGSLKLEN
760 770 780 790 800
TMELYRLSKA LADIVIPQEY GITKAEKLEI AKGYCTPLVR KIRSDLQRTQ
810 820 830 840 850
DDDTVNKLHP VYSRGVLSPE RHVRTRLYFT SESHVHSLLS ILRYGALCDD
860 870 880 890 900
SKDEQWKRAM DYLNVVNELN YMTQIVIMLY EDPNKDLSSE ERFHVELHFS
910 920 930 940 950
PGAKGCEEDK NLPSGYGYRP ASRENEGRRS LKTDDDEPHT SKRDEVDRAV
960 970 980 990 1000
MLFKPLVSEP IHIHRKSPLP RSRKITANEV VSENANYLRT PRNLVEQKQN
1010 1020 1030 1040 1050
PTVGFELYSM VPSICPLETL HNALFLKQVD DFLASIASPS TEVLRKVPEM
1060 1070 1080 1090 1100
SSMATRSSPG MRRKISLNTY TPTKILPTPP AALKSSKASS KAAAGGPSQA
1110 1120
MAPHTSSRKK SINSKTEGHE PKKSTGKKR
Length:1,129
Mass (Da):128,427
Last modified:July 27, 2011 - v3
Checksum:iA563826CC0085E3C
GO
Isoform 2 (identifier: Q6ZQB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     545-545: Missing.
     1051-1129: SSMATRSSPG...EPKKSTGKKR → CMEFTFIVT

Note: No experimental confirmation available.
Show »
Length:1,058
Mass (Da):121,181
Checksum:iEAD1A1398DB250E7
GO
Isoform 3 (identifier: Q6ZQB6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:1,123
Mass (Da):127,723
Checksum:i7B7941A5E5AB948F
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q9J4E9Q9J4_MOUSE
Inositol hexakisphosphate and dipho...
Ppip5k2
1,242Annotation score:
A0A087WPZ7A0A087WPZ7_MOUSE
Inositol hexakisphosphate and dipho...
Ppip5k2
92Annotation score:

Sequence cautioni

The sequence BAC97950 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197A → V in BAE38567 (PubMed:14621295).Curated1
Sequence conflicti582A → T in AAH53396 (PubMed:15489334).Curated1
Sequence conflicti729I → V in AAH53396 (PubMed:15489334).Curated1
Sequence conflicti1035S → P in AAH53396 (PubMed:15489334).Curated1
Sequence conflicti1109K → M in BAC97950 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0416181 – 6Missing in isoform 3. 1 Publication6
Alternative sequenceiVSP_030637545Missing in isoform 2. 1 Publication1
Alternative sequenceiVSP_0306381051 – 1129SSMAT…TGKKR → CMEFTFIVT in isoform 2. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129140 mRNA Translation: BAC97950.1 Different initiation.
AK138622 mRNA Translation: BAE23724.1
AK166095 mRNA Translation: BAE38567.1
AC099860 Genomic DNA No translation available.
AC162298 Genomic DNA No translation available.
BC053396 mRNA Translation: AAH53396.1
CCDSiCCDS35678.2 [Q6ZQB6-1]
RefSeqiNP_776121.4, NM_173760.5 [Q6ZQB6-1]
UniGeneiMm.220817
Mm.417682

Genome annotation databases

EnsembliENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648 [Q6ZQB6-1]
ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648 [Q6ZQB6-3]
GeneIDi227399
KEGGimmu:227399
UCSCiuc007cfk.4 mouse [Q6ZQB6-1]
uc007cfl.1 mouse [Q6ZQB6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129140 mRNA Translation: BAC97950.1 Different initiation.
AK138622 mRNA Translation: BAE23724.1
AK166095 mRNA Translation: BAE38567.1
AC099860 Genomic DNA No translation available.
AC162298 Genomic DNA No translation available.
BC053396 mRNA Translation: AAH53396.1
CCDSiCCDS35678.2 [Q6ZQB6-1]
RefSeqiNP_776121.4, NM_173760.5 [Q6ZQB6-1]
UniGeneiMm.220817
Mm.417682

3D structure databases

ProteinModelPortaliQ6ZQB6
SMRiQ6ZQB6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043401

PTM databases

iPTMnetiQ6ZQB6
PhosphoSitePlusiQ6ZQB6

Proteomic databases

PaxDbiQ6ZQB6
PeptideAtlasiQ6ZQB6
PRIDEiQ6ZQB6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042509; ENSMUSP00000043401; ENSMUSG00000040648 [Q6ZQB6-1]
ENSMUST00000171129; ENSMUSP00000132889; ENSMUSG00000040648 [Q6ZQB6-3]
GeneIDi227399
KEGGimmu:227399
UCSCiuc007cfk.4 mouse [Q6ZQB6-1]
uc007cfl.1 mouse [Q6ZQB6-2]

Organism-specific databases

CTDi23262
MGIiMGI:2142810 Ppip5k2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1057 Eukaryota
ENOG410XNSN LUCA
GeneTreeiENSGT00390000009048
HOGENOMiHOG000177917
HOVERGENiHBG108657
InParanoidiQ6ZQB6
KOiK13024
OMAiPWLFRER

Enzyme and pathway databases

ReactomeiR-MMU-1855167 Synthesis of pyrophosphates in the cytosol
SABIO-RKiQ6ZQB6

Miscellaneous databases

PROiPR:Q6ZQB6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040648 Expressed in 257 organ(s), highest expression level in pes
CleanExiMM_HISPPD1
ExpressionAtlasiQ6ZQB6 baseline and differential
GenevisibleiQ6ZQB6 MM

Family and domain databases

CDDicd07061 HP_HAP_like, 1 hit
InterProiView protein in InterPro
IPR033379 Acid_Pase_AS
IPR000560 His_Pase_clade-2
IPR037446 His_Pase_VIP1
IPR029033 His_PPase_superfam
PANTHERiPTHR12750 PTHR12750, 2 hits
PfamiView protein in Pfam
PF00328 His_Phos_2, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00616 HIS_ACID_PHOSPHAT_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVIP2_MOUSE
AccessioniPrimary (citable) accession number: Q6ZQB6
Secondary accession number(s): E9PVE9
, Q3TM75, Q3UUA3, Q7TPU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 101 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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