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Protein

Protein SMG5

Gene

Smg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNonsense-mediated mRNA decay

Enzyme and pathway databases

ReactomeiR-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SMG5
Alternative name(s):
EST1-like protein B
SMG-5 homolog
Gene namesi
Name:Smg5
Synonyms:Est1b, Kiaa1089
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2447364 Smg5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000763232 – 1017Protein SMG5Add BLAST1016

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei423PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6ZPY2
PaxDbiQ6ZPY2
PeptideAtlasiQ6ZPY2
PRIDEiQ6ZPY2

PTM databases

iPTMnetiQ6ZPY2
PhosphoSitePlusiQ6ZPY2

Expressioni

Gene expression databases

BgeeiENSMUSG00000001415 Expressed in 279 organ(s), highest expression level in adipose tissue
ExpressionAtlasiQ6ZPY2 baseline and differential
GenevisibleiQ6ZPY2 MM

Interactioni

Subunit structurei

Interacts with TERT, PPP2CA and SMG1. Part of a complex that contains SMG1, SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1. Not detected in complexes that contain unphosphorylated UPF1.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001451

Structurei

3D structure databases

ProteinModelPortaliQ6ZPY2
SMRiQ6ZPY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini873 – 996PINcAdd BLAST124

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili798 – 842Sequence analysisAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi459 – 463Poly-Arg5

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410INQY Eukaryota
ENOG410XPF4 LUCA
GeneTreeiENSGT00560000077120
HOGENOMiHOG000261635
HOVERGENiHBG082268
InParanoidiQ6ZPY2
KOiK11125
OMAiGMAQNQL
OrthoDBiEOG091G0188
PhylomeDBiQ6ZPY2
TreeFamiTF327119

Family and domain databases

InterProiView protein in InterPro
IPR018834 DNA/RNA-bd_Est1-type
IPR019458 EST1
IPR002716 PIN_dom
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF10374 EST1, 1 hit
PF10373 EST1_DNA_bind, 2 hits
PF13638 PIN_4, 1 hit
SMARTiView protein in SMART
SM00670 PINc, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6ZPY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQGPPPGES SEPEAKVLHT KRLYRAVVEA VHRLDLILCN KAAYQEVFKP
60 70 80 90 100
ENVSLRNKLR ELCVKLMFLH PVDYGRKAEE LLWRKVYYEV IQLIKTNKKH
110 120 130 140 150
IHSRSTLECA YRTHLVAGIG FYQHLLLYIQ SHYQLELQCC IDWTHVTDPL
160 170 180 190 200
MGFKKPVSAS GKEMDWAQMA CHRCLVYLGD LSRYQNELAG VDTELLAERF
210 220 230 240 250
YYQALSVAPQ IGMPFNQLGT LAGSKYYNVE AMYCYLRCIQ SEVSFEGAYG
260 270 280 290 300
NLKRLYDKAA KMYHQLKKSE TRKLSPSKKR CKDIKRLLVN FMYLQSLLQP
310 320 330 340 350
KSSSVDSELT SLCQSVLEDF NLCLFYLPSS PNLGLTNEDE EECESGYAFL
360 370 380 390 400
PDLLIFQMAI ICLMGVHSLK RAGSKHYSAA IAFTLALFSH LINHVNIRLQ
410 420 430 440 450
AELEEGENPV SAFQSDGTDE PESKEALEKE EPEPEPPTVV PQADEGRKSR
460 470 480 490 500
KHSRLSCLRR RRRHHPPKAG DDSDLSEGFE SDSSHDSAQA SDGSDSGSDK
510 520 530 540 550
SLEGRGTAFD AETDSEMNSQ ESRSDLEDME DEEGTRSPAQ EPPQARSEVP
560 570 580 590 600
DSLNGPLGPS EASIASNLQA MSTQMFQTKR CFRLAPTFSN LLLQPTTEPN
610 620 630 640 650
SVASHRPCVN GDMDKPLEPA SEDGSESEGS ESSNRSCRNE RSLQEKLQAL
660 670 680 690 700
MAEGLLPAVK VFLDWLRTNP DLIIVCAQSS QSLWNRLSVL LNLLPASAEL
710 720 730 740 750
QDSGLALCSE VQGLLEGCEL PDLPASLLLP EDMALRNLPP LRAAHRRFNF
760 770 780 790 800
DADRPLLSAL EESVVRICCI RSFGHFVARL QGSILQFNPE VGIFVSIAQS
810 820 830 840 850
EQESLLQQAQ AQFRMAEEEA RRNRLMRDMA QLRLQLEVSQ LEGSLQQPKA
860 870 880 890 900
QSAMSPYLIP DTQALCYHLP LIRQLATSGR FIIIIPRTVI DGLDLLKKEQ
910 920 930 940 950
PGARDGIRYL EAEFKKGNRY IRCQKEVGKS FERHKLKRQD ADAWTLYKIL
960 970 980 990 1000
DSCRQLTLAQ GAGEEDPSGM VTIITGLHLD SPSALSGPMQ AALQAAAHAS
1010
VDVKNVLDFY RQWKEIG
Length:1,017
Mass (Da):114,071
Last modified:December 20, 2005 - v2
Checksum:i9925688D6FAFAF5C
GO
Isoform 2 (identifier: Q6ZPY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     620-702: ASEDGSESEG...LLPASAELQD → GAWVHESPPS...GSQKLGIMFL
     703-1017: Missing.

Show »
Length:702
Mass (Da):78,613
Checksum:iFD7AB5774BDF168D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YWQ7A0A0A6YWQ7_MOUSE
Protein SMG5
Smg5
180Annotation score:

Sequence cautioni

The sequence BAC98096 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti77K → R in strain: B5/EGFP. 1 Publication1
Natural varianti166W → L in strain: Czech II. 1 Publication1
Natural varianti499D → E in strain: Czech II. 1 Publication1
Natural varianti503E → G in strain: Czech II. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016590620 – 702ASEDG…AELQD → GAWVHESPPSRRVTSPLGFC CHCGILVLCTYSFPASRTFA VLLLSTADCSSFTLFSPHIG GCTSFPHGIQLPEGSQKLGI MFL in isoform 2. 1 PublicationAdd BLAST83
Alternative sequenceiVSP_016591703 – 1017Missing in isoform 2. 1 PublicationAdd BLAST315

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129286 mRNA Translation: BAC98096.1 Different initiation.
BC024683 mRNA Translation: AAH24683.3
BC100408 mRNA Translation: AAI00409.1
CCDSiCCDS38480.1 [Q6ZPY2-1]
RefSeqiNP_839977.2, NM_178246.3 [Q6ZPY2-1]
UniGeneiMm.439830

Genome annotation databases

EnsembliENSMUST00000001451; ENSMUSP00000001451; ENSMUSG00000001415 [Q6ZPY2-1]
GeneIDi229512
KEGGimmu:229512
UCSCiuc008pus.1 mouse [Q6ZPY2-2]
uc008put.1 mouse [Q6ZPY2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129286 mRNA Translation: BAC98096.1 Different initiation.
BC024683 mRNA Translation: AAH24683.3
BC100408 mRNA Translation: AAI00409.1
CCDSiCCDS38480.1 [Q6ZPY2-1]
RefSeqiNP_839977.2, NM_178246.3 [Q6ZPY2-1]
UniGeneiMm.439830

3D structure databases

ProteinModelPortaliQ6ZPY2
SMRiQ6ZPY2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001451

PTM databases

iPTMnetiQ6ZPY2
PhosphoSitePlusiQ6ZPY2

Proteomic databases

EPDiQ6ZPY2
PaxDbiQ6ZPY2
PeptideAtlasiQ6ZPY2
PRIDEiQ6ZPY2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001451; ENSMUSP00000001451; ENSMUSG00000001415 [Q6ZPY2-1]
GeneIDi229512
KEGGimmu:229512
UCSCiuc008pus.1 mouse [Q6ZPY2-2]
uc008put.1 mouse [Q6ZPY2-1]

Organism-specific databases

CTDi23381
MGIiMGI:2447364 Smg5
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INQY Eukaryota
ENOG410XPF4 LUCA
GeneTreeiENSGT00560000077120
HOGENOMiHOG000261635
HOVERGENiHBG082268
InParanoidiQ6ZPY2
KOiK11125
OMAiGMAQNQL
OrthoDBiEOG091G0188
PhylomeDBiQ6ZPY2
TreeFamiTF327119

Enzyme and pathway databases

ReactomeiR-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

PROiPR:Q6ZPY2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001415 Expressed in 279 organ(s), highest expression level in adipose tissue
ExpressionAtlasiQ6ZPY2 baseline and differential
GenevisibleiQ6ZPY2 MM

Family and domain databases

InterProiView protein in InterPro
IPR018834 DNA/RNA-bd_Est1-type
IPR019458 EST1
IPR002716 PIN_dom
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF10374 EST1, 1 hit
PF10373 EST1_DNA_bind, 2 hits
PF13638 PIN_4, 1 hit
SMARTiView protein in SMART
SM00670 PINc, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMG5_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPY2
Secondary accession number(s): Q497S2, Q8R3S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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