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Protein

Formin-like protein 3

Gene

Fmnl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. Required for developmental angiogenesis. In this process, required for microtubule reorganization and for efficient endothelial cell elongation. In quiescent endothelial cells, triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.By similarity

GO - Molecular functioni

  • actin binding Source: InterPro
  • GTPase activating protein binding Source: UniProtKB
  • Rho GTPase binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processAngiogenesis

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
Formin-like protein 3
Gene namesi
Name:Fmnl3
Synonyms:Frl2, Kiaa2014
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109569 Fmnl3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002890962 – 1028Formin-like protein 3Add BLAST1027

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei95PhosphothreonineCombined sources1
Modified residuei174PhosphoserineCombined sources1
Modified residuei1014PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ6ZPF4
PeptideAtlasiQ6ZPF4
PRIDEiQ6ZPF4

PTM databases

iPTMnetiQ6ZPF4
PhosphoSitePlusiQ6ZPF4

Expressioni

Gene expression databases

BgeeiENSMUSG00000023008 Expressed in 185 organ(s), highest expression level in primary oocyte
CleanExiMM_FMNL3
ExpressionAtlasiQ6ZPF4 baseline and differential
GenevisibleiQ6ZPF4 MM

Interactioni

Subunit structurei

Interacts with SRGAP2 (via SH3 domain).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681353EBI-16027300,EBI-367540From Oryctolagus cuniculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204547, 2 interactors
DIPiDIP-32010N
IntActiQ6ZPF4, 4 interactors
STRINGi10090.ENSMUSP00000085566

Structurei

Secondary structure

11028
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ6ZPF4
SMRiQ6ZPF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 472GBD/FH3PROSITE-ProRule annotationAdd BLAST447
Domaini561 – 951FH2PROSITE-ProRule annotationAdd BLAST391
Domaini986 – 1018DADAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi499 – 548Pro-richAdd BLAST50

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated

Phylogenomic databases

eggNOGiKOG1923 Eukaryota
ENOG410Y3DT LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000231209
HOVERGENiHBG053118
InParanoidiQ6ZPF4
OMAiCSVMYST
OrthoDBiEOG091G01GP
PhylomeDBiQ6ZPF4
TreeFamiTF325155

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR027655 Fmnl3
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF230 PTHR23213:SF230, 1 hit
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 2 hits
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6ZPF4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGNLESTDGG PGEPPSVPLL LPPGKTPMPE PCELEERFAL VLSSMNLPPD
60 70 80 90 100
KARLLRQYDN EKKWDLICDQ ERFQVKNPPH TYIQKLQSFL DPNVTRKKFR
110 120 130 140 150
RRVQESTKVL RELEISLRTN HIGWVREFLN DENKGLDVLV DYLSFAQCSV
160 170 180 190 200
MFDFEGLESG DDGAFDKLRS WSRSIEDLQP PNALSAPFTN SLARSARQSV
210 220 230 240 250
LRYSTLPGRR ALKNSRLVSQ KDDVHVCILC LRAIMNYQYG FNLVMSHPHA
260 270 280 290 300
VNEIALSLNN KNPRTKALVL ELLAAVCLVR GGHEIILAAF DNFKEVCKEL
310 320 330 340 350
HRFEKLMEYF RNEDSNIDFM VACMQFINIV VHSVEDMNFR VHLQYEFTKL
360 370 380 390 400
GLEEFLQKSR HTESEKLQVQ IQAYLDNVFD VGGLLEDAET KNVALEKVEE
410 420 430 440 450
LEEHVSHLTE KLLDLENENM MRVAELEKQL LQREKELESI KETYENTSNQ
460 470 480 490 500
VHTLRRLIKE KEEAFQRRCH LEPSARGLES MGGEALARVG PTELTEGIPP
510 520 530 540 550
SDLDLLAPAP PTEETLPLPP PPAPPLPPPP PPLPDKCPPA PPLPGAAPSV
560 570 580 590 600
VLTVGLSAIR IKKPIKTKFR LPVFNWTALK PNQINGTVFS ELDDEKILED
610 620 630 640 650
LDLDRFEELF KTKAQGPALD LICSKNKTAQ KAASKVTLLE ANRAKNLAIT
660 670 680 690 700
LRKAGRSAEE ICRAIHTFDL QTLPVDFVEC LMRFLPTEAE VKLLRQYERE
710 720 730 740 750
RQPLEELAAE DRFMLLFSKV ERLTQRMAGM AFLGNFQDNL QMLTPQLNAI
760 770 780 790 800
IAASASVKSS QKLKQMLEII LALGNYMNSS KRGAVYGFKL QSLDLLLDTK
810 820 830 840 850
STDRKMTLLH FIALTVKEKY PELANFWQEL HFVEKAAAVS LENVLLDVKE
860 870 880 890 900
LGRGMELIRR ECSIHDNSVL RNFLSTNEGK LDKLQRDAKT AEEAYNAVVR
910 920 930 940 950
YFGESPKTTP PSVFFPVFVR FIRSYKEAEQ ENEARKKQEE VMREKQLAQE
960 970 980 990 1000
AKKLDAKTPS QRNKWQQQEL IAELRRRQAK EHRPVYEGKD GTIEDIITVL
1010 1020
KSVPFTARTA KRGSRFFCDA AHHDESNC
Length:1,028
Mass (Da):117,169
Last modified:May 29, 2007 - v2
Checksum:iBBA0436746934A3B
GO
Isoform 2 (identifier: Q6ZPF4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     152-202: Missing.
     999-1028: VLKSVPFTARTAKRGSRFFCDAAHHDESNC → GFNHQRMVVHSQVRSAVPPSGPPRAPGPH

Show »
Length:976
Mass (Da):111,279
Checksum:i702206CD74A93F8B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z7A7D3Z7A7_MOUSE
Formin-like protein 3
Fmnl3
1,027Annotation score:

Sequence cautioni

The sequence BAC98283 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025894152 – 202Missing in isoform 2. 1 PublicationAdd BLAST51
Alternative sequenceiVSP_025895999 – 1028VLKSV…DESNC → GFNHQRMVVHSQVRSAVPPS GPPRAPGPH in isoform 2. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129473 mRNA Translation: BAC98283.1 Different initiation.
AK155331 mRNA Translation: BAE33197.1
AK170744 mRNA Translation: BAE41997.1
BC131961 mRNA Translation: AAI31962.1
CCDSiCCDS27819.1 [Q6ZPF4-1]
CCDS79408.1 [Q6ZPF4-2]
RefSeqiNP_001297551.1, NM_001310622.1
NP_001297552.1, NM_001310623.1 [Q6ZPF4-2]
NP_035841.1, NM_011711.2 [Q6ZPF4-1]
UniGeneiMm.29043

Genome annotation databases

EnsembliENSMUST00000081224; ENSMUSP00000079984; ENSMUSG00000023008 [Q6ZPF4-2]
ENSMUST00000088233; ENSMUSP00000085566; ENSMUSG00000023008 [Q6ZPF4-1]
GeneIDi22379
KEGGimmu:22379
UCSCiuc007xpj.1 mouse [Q6ZPF4-1]
uc007xpl.1 mouse [Q6ZPF4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129473 mRNA Translation: BAC98283.1 Different initiation.
AK155331 mRNA Translation: BAE33197.1
AK170744 mRNA Translation: BAE41997.1
BC131961 mRNA Translation: AAI31962.1
CCDSiCCDS27819.1 [Q6ZPF4-1]
CCDS79408.1 [Q6ZPF4-2]
RefSeqiNP_001297551.1, NM_001310622.1
NP_001297552.1, NM_001310623.1 [Q6ZPF4-2]
NP_035841.1, NM_011711.2 [Q6ZPF4-1]
UniGeneiMm.29043

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EAHX-ray3.40A/B/C/E555-954[»]
ProteinModelPortaliQ6ZPF4
SMRiQ6ZPF4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204547, 2 interactors
DIPiDIP-32010N
IntActiQ6ZPF4, 4 interactors
STRINGi10090.ENSMUSP00000085566

PTM databases

iPTMnetiQ6ZPF4
PhosphoSitePlusiQ6ZPF4

Proteomic databases

PaxDbiQ6ZPF4
PeptideAtlasiQ6ZPF4
PRIDEiQ6ZPF4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081224; ENSMUSP00000079984; ENSMUSG00000023008 [Q6ZPF4-2]
ENSMUST00000088233; ENSMUSP00000085566; ENSMUSG00000023008 [Q6ZPF4-1]
GeneIDi22379
KEGGimmu:22379
UCSCiuc007xpj.1 mouse [Q6ZPF4-1]
uc007xpl.1 mouse [Q6ZPF4-2]

Organism-specific databases

CTDi91010
MGIiMGI:109569 Fmnl3
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1923 Eukaryota
ENOG410Y3DT LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000231209
HOVERGENiHBG053118
InParanoidiQ6ZPF4
OMAiCSVMYST
OrthoDBiEOG091G01GP
PhylomeDBiQ6ZPF4
TreeFamiTF325155

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins

Miscellaneous databases

PROiPR:Q6ZPF4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023008 Expressed in 185 organ(s), highest expression level in primary oocyte
CleanExiMM_FMNL3
ExpressionAtlasiQ6ZPF4 baseline and differential
GenevisibleiQ6ZPF4 MM

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR027655 Fmnl3
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF230 PTHR23213:SF230, 1 hit
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 2 hits
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFMNL3_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPF4
Secondary accession number(s): Q3TCF9, Q3U2E4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 7, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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