Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myotubularin-related protein 5

Gene

Sbf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. Alternatively proposed to function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity).By similarity

GO - Molecular functioni

  • pseudophosphatase activity Source: MGI
  • Rab guanyl-nucleotide exchange factor activity Source: MGI

GO - Biological processi

  • regulation of cell growth Source: MGI
  • regulation of GTPase activity Source: InterPro
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-1483248 Synthesis of PIPs at the ER membrane
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin-related protein 5
Alternative name(s):
SET-binding factor 1
Short name:
Sbf1
Gene namesi
Name:Sbf1
Synonyms:Kiaa3020, Mtmr5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1925230 Sbf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002934761 – 1867Myotubularin-related protein 5Add BLAST1867

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1120PhosphoserineBy similarity1
Modified residuei1137PhosphothreonineBy similarity1
Modified residuei1222N6-methyllysineBy similarity1
Modified residuei1746PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ6ZPE2
PaxDbiQ6ZPE2
PeptideAtlasiQ6ZPE2
PRIDEiQ6ZPE2

PTM databases

iPTMnetiQ6ZPE2
PhosphoSitePlusiQ6ZPE2

Expressioni

Gene expression databases

BgeeiENSMUSG00000036529 Expressed in 233 organ(s), highest expression level in cerebellum
CleanExiMM_SBF1
ExpressionAtlasiQ6ZPE2 baseline and differential
GenevisibleiQ6ZPE2 MM

Interactioni

Subunit structurei

Interacts with the SET domain of KMT2A/MLL1. Interacts with SUV39H1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219065, 4 interactors
IntActiQ6ZPE2, 5 interactors
STRINGi10090.ENSMUSP00000118107

Structurei

Secondary structure

11867
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ6ZPE2
SMRiQ6ZPE2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6ZPE2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 184uDENNPROSITE-ProRule annotationAdd BLAST178
Domaini203 – 336cDENNPROSITE-ProRule annotationAdd BLAST134
Domaini338 – 439dDENNPROSITE-ProRule annotationAdd BLAST102
Domaini880 – 968GRAMAdd BLAST89
Domaini1120 – 1596Myotubularin phosphatasePROSITE-ProRule annotationAdd BLAST477
Domaini1761 – 1865PHPROSITE-ProRule annotationAdd BLAST105

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1090 Eukaryota
KOG2080 Eukaryota
ENOG410XTJ1 LUCA
GeneTreeiENSGT00760000118832
HOGENOMiHOG000044360
HOVERGENiHBG052527
InParanoidiQ6ZPE2
KOiK18061
OMAiDQATAKM
OrthoDBiEOG091G00CQ
TreeFamiTF318583

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR001194 cDENN_dom
IPR005112 dDENN_dom
IPR004182 GRAM
IPR030574 MTMR5
IPR010569 Myotubularin-like_Pase_dom
IPR030564 Myotubularin_fam
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR029021 Prot-tyrosine_phosphatase-like
IPR022096 SBF1/SBF2
IPR037516 Tripartite_DENN
IPR005113 uDENN_dom
PANTHERiPTHR10807 PTHR10807, 1 hit
PTHR10807:SF43 PTHR10807:SF43, 1 hit
PfamiView protein in Pfam
PF02141 DENN, 1 hit
PF02893 GRAM, 1 hit
PF06602 Myotub-related, 1 hit
PF00169 PH, 1 hit
PF12335 SBF2, 1 hit
PF03456 uDENN, 1 hit
SMARTiView protein in SMART
SM00801 dDENN, 1 hit
SM00799 DENN, 1 hit
SM00568 GRAM, 1 hit
SM00233 PH, 1 hit
SM00800 uDENN, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50211 DENN, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS51339 PPASE_MYOTUBULARIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6ZPE2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MARLADYFVL VAFGPHPRGS GEGQGQILQR FPEKDWEDNP FPQGIELFCQ
60 70 80 90 100
PSGWQLCPER NPPTFFVAVL TDINSERHYC ACLTFWEPVE STQEVVCTDN
110 120 130 140 150
ATEKEEEADG GGQARLSSTA PAQPGQLFAP KTLVLVSRLD HAEVFRNSLG
160 170 180 190 200
LIYAIHVEGL NVSLENVIGN LLTCTVPLAG GSQRTISLGA GDRQVIQTPL
210 220 230 240 250
VDSLPVSRCS VALLFRQLGI TNVLSLFCAA LTEHKVLFLS RSYQRLADAC
260 270 280 290 300
RGLLALLFPL RYSFTYVPIL PAQLLEVLST PTPFIIGVNA AFQAETQELL
310 320 330 340 350
DVIVADLDGG TVTVPECVHI PPLPEPLQSQ THNVLSMVLD PELELADLAF
360 370 380 390 400
PPPTTSASSL KMQDKELRAV FLRLFAQLLQ GYRWCLHIVR IHPEPVIRFH
410 420 430 440 450
KAAFLGQRGL VEDDFLMKVL EGMAFAGFVS ERGVPYRATD LFDELVAHEV
460 470 480 490 500
ARMRADESHP HRVLRHVQEL AEQLYKNENP YPAVAMHKVQ RPGEASHLRR
510 520 530 540 550
THRPFPRLDE GTIQWIVDQA AAKMQGAPPA VKAERRSTVP SGPPMTAILE
560 570 580 590 600
RCSGPHINSA RRLEVVRNCI SYVFEGKMLE AKKLLPAVLR ALKGRAARRC
610 620 630 640 650
LAHELHLHVQ QNRAVLDHQQ FDFVVRMMNC CLQDCTSLDE HGIASALLPL
660 670 680 690 700
VTAFCRKLSP GVTQFAYSCV QEHVVWSTPQ FWEAMFYGDV QTHIRALYLE
710 720 730 740 750
PSDGVSPTQE TGEAQSQDDE RSALDVASEQ RRLWPTLSRE KQQELVQKEE
760 770 780 790 800
STVFSQAIHY ANRMSYLLLP LDSSKSRLLR ERAGLGDLES ASNSLVTNSM
810 820 830 840 850
AGSVAESYDT ESGFEDAETC DVAGAVVRFI NRFVDKVCTE SGVTSDHLKG
860 870 880 890 900
LHVMVPDIVQ MHIETLEAVH RESKRLPPIQ KPKLLRPRLL PGEECVLDGL
910 920 930 940 950
RVYLLPDGRE EGVGGSGGGP ALLPAEGAVF LTTYRVIFTG MPTDPLVGEQ
960 970 980 990 1000
VVVRSFPVAA LTKEKRISVQ TPVDQLLQDG LQLRSCTFQL LKMAFDEEVG
1010 1020 1030 1040 1050
SDSAELFRKQ LHKLRYPPDI RATFAFTLGS AHTPGRPPRV TKDKGPSFRT
1060 1070 1080 1090 1100
LSRNLMKNAK KTIGRQYVTR KKYNPPGWEH RGQPPPEDQE DEISVSEELE
1110 1120 1130 1140 1150
PSTLTPSSAL KPSDRMTMSS LVERACCRDY QRLGLGTLSS SLSRAKSEPF
1160 1170 1180 1190 1200
RISPVNRMYA ICRSYPGLLI VPQSIQDNAL QRVSRCYRQN RFPVVCWRSG
1210 1220 1230 1240 1250
RSKAVLLRSG GLHGKGVVGL FKAQNTPSPG QAQADSSSLE QEKYLQAVVS
1260 1270 1280 1290 1300
SMPRYADSSG RNTLSSFSSA HMGGHGKWSS VRASGRSSGL GSDVGSRLAG
1310 1320 1330 1340 1350
RDLLSTPHTN GAPPDSGFLR PQRAALYIIG DKAQLKGVRP DPLQQWELVP
1360 1370 1380 1390 1400
IEVFEARQVK ASFKKLLKAC VPGCPATEPS PASFLRSLED SEWLIQIHKL
1410 1420 1430 1440 1450
LQISVLVVEL LDSGSSVLVS LEDGWDITTQ VVSLVQLLSD PFYRTLEGFR
1460 1470 1480 1490 1500
LLVEKEWLSF GHRFSHRGAH TLAGQSSGFT PVFLQFLDCV HQVHLQFPME
1510 1520 1530 1540 1550
FEFSQFYLKF LGYHHTSRRF RTFLLDSDYE RIELGLLYEE KGERRGQLAC
1560 1570 1580 1590 1600
KSVWEYVDRL SKRTPMFYNY TYAPEDTEVL RPYSNVSNLK VWDFYTEETL
1610 1620 1630 1640 1650
AEGPPYDWEL AQGPPEPPEE ERPDGGAPQS RRRVVWPCYD SRPRVQPDAI
1660 1670 1680 1690 1700
SRLLEELQRL ETELGRPSER WKDTWDRVKA AQRLESRQDG RGTPSSLLVS
1710 1720 1730 1740 1750
AVPHHRRSLG VYLQEGPVGS TLSLSLDSDQ SSGSTTSSSR QAARRSTSTL
1760 1770 1780 1790 1800
YSQFQTAESE NRSYEGILYK KGAFMKPWKA RWFVLDKTKH QLRYYDHRMD
1810 1820 1830 1840 1850
TECKGVIDLA EVEAVAPGTP TIGAPKTVDE KAFFDVKTTR RVYNFCAQDV
1860
PSAQQWVDRI QSCLSDA
Length:1,867
Mass (Da):208,693
Last modified:July 10, 2007 - v2
Checksum:i16C7033FAA62CB1A
GO
Isoform 2 (identifier: Q6ZPE2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1275-1275: H → HVPSPRARVTTLSNPLAASASRWTASR

Show »
Length:1,893
Mass (Da):211,428
Checksum:iD4F26DD11889F5D6
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BKD9H3BKD9_MOUSE
Myotubularin-related protein 5
Sbf1
415Annotation score:
F6S251F6S251_MOUSE
Myotubularin-related protein 5
Sbf1
176Annotation score:
D6RG52D6RG52_MOUSE
Myotubularin-related protein 5
Sbf1
403Annotation score:
F7AWK3F7AWK3_MOUSE
Myotubularin-related protein 5
Sbf1
257Annotation score:

Sequence cautioni

The sequence AAH29156 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC98295 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317C → R in AAI72094 (PubMed:15489334).Curated1
Sequence conflicti1347E → D in AAI57936 (PubMed:15489334).Curated1
Sequence conflicti1521R → W in BAC36139 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0475231275H → HVPSPRARVTTLSNPLAASA SRWTASR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129485 mRNA Translation: BAC98295.1 Different initiation.
AC160538 Genomic DNA No translation available.
BC029156 mRNA Translation: AAH29156.1 Different initiation.
BC098209 mRNA Translation: AAH98209.1
BC157935 mRNA Translation: AAI57936.1
BC158013 mRNA Translation: AAI58014.1
BC172094 mRNA Translation: AAI72094.1
AK076039 mRNA Translation: BAC36139.1
CCDSiCCDS37175.2 [Q6ZPE2-1]
CCDS49699.1 [Q6ZPE2-2]
RefSeqiNP_001074499.2, NM_001081030.2 [Q6ZPE2-1]
NP_001164032.1, NM_001170561.1 [Q6ZPE2-2]
UniGeneiMm.35483

Genome annotation databases

EnsembliENSMUST00000123791; ENSMUSP00000120725; ENSMUSG00000036529 [Q6ZPE2-1]
ENSMUST00000144585; ENSMUSP00000118107; ENSMUSG00000036529 [Q6ZPE2-2]
GeneIDi77980
KEGGimmu:77980
UCSCiuc007xfz.1 mouse [Q6ZPE2-1]
uc011zxu.1 mouse [Q6ZPE2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129485 mRNA Translation: BAC98295.1 Different initiation.
AC160538 Genomic DNA No translation available.
BC029156 mRNA Translation: AAH29156.1 Different initiation.
BC098209 mRNA Translation: AAH98209.1
BC157935 mRNA Translation: AAI57936.1
BC158013 mRNA Translation: AAI58014.1
BC172094 mRNA Translation: AAI72094.1
AK076039 mRNA Translation: BAC36139.1
CCDSiCCDS37175.2 [Q6ZPE2-1]
CCDS49699.1 [Q6ZPE2-2]
RefSeqiNP_001074499.2, NM_001081030.2 [Q6ZPE2-1]
NP_001164032.1, NM_001170561.1 [Q6ZPE2-2]
UniGeneiMm.35483

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V5UNMR-A1762-1865[»]
ProteinModelPortaliQ6ZPE2
SMRiQ6ZPE2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219065, 4 interactors
IntActiQ6ZPE2, 5 interactors
STRINGi10090.ENSMUSP00000118107

PTM databases

iPTMnetiQ6ZPE2
PhosphoSitePlusiQ6ZPE2

Proteomic databases

EPDiQ6ZPE2
PaxDbiQ6ZPE2
PeptideAtlasiQ6ZPE2
PRIDEiQ6ZPE2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000123791; ENSMUSP00000120725; ENSMUSG00000036529 [Q6ZPE2-1]
ENSMUST00000144585; ENSMUSP00000118107; ENSMUSG00000036529 [Q6ZPE2-2]
GeneIDi77980
KEGGimmu:77980
UCSCiuc007xfz.1 mouse [Q6ZPE2-1]
uc011zxu.1 mouse [Q6ZPE2-2]

Organism-specific databases

CTDi6305
MGIiMGI:1925230 Sbf1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1090 Eukaryota
KOG2080 Eukaryota
ENOG410XTJ1 LUCA
GeneTreeiENSGT00760000118832
HOGENOMiHOG000044360
HOVERGENiHBG052527
InParanoidiQ6ZPE2
KOiK18061
OMAiDQATAKM
OrthoDBiEOG091G00CQ
TreeFamiTF318583

Enzyme and pathway databases

ReactomeiR-MMU-1483248 Synthesis of PIPs at the ER membrane
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiSbf1 mouse
EvolutionaryTraceiQ6ZPE2
PROiPR:Q6ZPE2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036529 Expressed in 233 organ(s), highest expression level in cerebellum
CleanExiMM_SBF1
ExpressionAtlasiQ6ZPE2 baseline and differential
GenevisibleiQ6ZPE2 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR001194 cDENN_dom
IPR005112 dDENN_dom
IPR004182 GRAM
IPR030574 MTMR5
IPR010569 Myotubularin-like_Pase_dom
IPR030564 Myotubularin_fam
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR029021 Prot-tyrosine_phosphatase-like
IPR022096 SBF1/SBF2
IPR037516 Tripartite_DENN
IPR005113 uDENN_dom
PANTHERiPTHR10807 PTHR10807, 1 hit
PTHR10807:SF43 PTHR10807:SF43, 1 hit
PfamiView protein in Pfam
PF02141 DENN, 1 hit
PF02893 GRAM, 1 hit
PF06602 Myotub-related, 1 hit
PF00169 PH, 1 hit
PF12335 SBF2, 1 hit
PF03456 uDENN, 1 hit
SMARTiView protein in SMART
SM00801 dDENN, 1 hit
SM00799 DENN, 1 hit
SM00568 GRAM, 1 hit
SM00233 PH, 1 hit
SM00800 uDENN, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50211 DENN, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS51339 PPASE_MYOTUBULARIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTMR5_MOUSE
AccessioniPrimary (citable) accession number: Q6ZPE2
Secondary accession number(s): B2RXQ1
, B2RXX4, B7ZWK2, Q4QQM2, Q8BK68, Q8K2Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 7, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again