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Protein

Beclin 1-associated autophagy-related key regulator

Gene

ATG14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604). Modulates the hepatic lipid metabolism (By similarity).By similarity6 Publications

GO - Molecular functioni

  • GTPase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

BRENDAi2.7.1.137 2681
ReactomeiR-HSA-1632852 Macroautophagy
SIGNORiQ6ZNE5

Protein family/group databases

TCDBi9.A.15.2.1 the autophagy-related phagophore-formation transporter (apt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Beclin 1-associated autophagy-related key regulator1 Publication
Short name:
Barkor1 Publication
Alternative name(s):
Autophagy-related protein 14-like protein1 Publication
Short name:
Atg14L1 Publication
Gene namesi
Name:ATG141 Publication
Synonyms:ATG14L1 Publication, KIAA0831
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000126775.8
HGNCiHGNC:19962 ATG14
MIMi613515 gene
neXtProtiNX_Q6ZNE5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-46; A-55 and A-58. 2 Publications1
Mutagenesisi46C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-43; A-55 and A-58. 2 Publications1
Mutagenesisi55C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-43; A-46 and A-58. 2 Publications1
Mutagenesisi58C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-43; A-46 and A-55. 2 Publications1

Organism-specific databases

DisGeNETi22863
OpenTargetsiENSG00000126775
PharmGKBiPA165478560

Polymorphism and mutation databases

BioMutaiATG14
DMDMi67461020

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000507741 – 492Beclin 1-associated autophagy-related key regulatorAdd BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Modified residuei416PhosphoserineCombined sources1
Modified residuei429PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6ZNE5
MaxQBiQ6ZNE5
PaxDbiQ6ZNE5
PeptideAtlasiQ6ZNE5
PRIDEiQ6ZNE5
ProteomicsDBi68016
68017 [Q6ZNE5-2]

PTM databases

iPTMnetiQ6ZNE5
PhosphoSitePlusiQ6ZNE5

Expressioni

Gene expression databases

BgeeiENSG00000126775 Expressed in 229 organ(s), highest expression level in female gonad
CleanExiHS_KIAA0831
GenevisibleiQ6ZNE5 HS

Organism-specific databases

HPAiCAB037242

Interactioni

Subunit structurei

Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion (PubMed:25686604). Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex I (PI3KC3-C1) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with ATG14 (PubMed:18843052, PubMed:19050071, PubMed:19270696, PubMed:22314358, PubMed:23878393). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). PI3KC3-C1 can associate with further regulatory subunits. Interacts with PIK3CB (By similarity). Interacts (via coiled-coil domain) with BECN2 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (PubMed:23954414, PubMed:28218432). Interacts with the STX17-SNAP29 binary t-SNARE complex (PubMed:25686604). Interacts with NRBF2 (By similarity).By similarityCurated9 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116531, 22 interactors
ComplexPortaliCPX-73 Phosphatidylinositol 3-kinase complex, class III, ATG14 variant
CORUMiQ6ZNE5
DIPiDIP-48651N
IntActiQ6ZNE5, 11 interactors
MINTiQ6ZNE5
STRINGi9606.ENSP00000247178

Structurei

3D structure databases

ProteinModelPortaliQ6ZNE5
SMRiQ6ZNE5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 58Cysteine repeatsAdd BLAST16
Regioni413 – 492BATSBy similarityAdd BLAST80

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili71 – 180Sequence analysisAdd BLAST110

Domaini

The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.1 Publication
The final 80 residues in the C-terminus define a minimum required region for autophagosome binding called BATS.2 Publications
The N-terminal cysteine repeats are required for proper localization to the endoplasmic reticulum.1 Publication

Sequence similaritiesi

Belongs to the ATG14 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4398 Eukaryota
ENOG410Y9W5 LUCA
GeneTreeiENSGT00390000011854
HOVERGENiHBG081828
InParanoidiQ6ZNE5
KOiK17889
OMAiNTSYIRG
OrthoDBiEOG091G074J
PhylomeDBiQ6ZNE5
TreeFamiTF323392

Family and domain databases

InterProiView protein in InterPro
IPR018791 UV_resistance/autophagy_Atg14
PfamiView protein in Pfam
PF10186 Atg14, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q6ZNE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASPSGKGAR ALEAPGCGPR PLARDLVDSV DDAEGLYVAV ERCPLCNTTR
60 70 80 90 100
RRLTCAKCVQ SGDFVYFDGR DRERFIDKKE RLSRLKSKQE EFQKEVLKAM
110 120 130 140 150
EGKWITDQLR WKIMSCKMRI EQLKQTICKG NEEMEKNSEG LLKTKEKNQK
160 170 180 190 200
LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT IDLRSHYERL ANLRRSHILE
210 220 230 240 250
LTSVIFPIEE VKTGVRDPAD VSSESDSAMT SSTVSKLAEA RRTTYLSGRW
260 270 280 290 300
VCDDHNGDTS ISITGPWISL PNNGDYSAYY SWVEEKKTTQ GPDMEQSNPA
310 320 330 340 350
YTISAALCYA TQLVNILSHI LDVNLPKKLC NSEFCGENLS KQKFTRAVKK
360 370 380 390 400
LNANILYLCF SQHVNLDQLQ PLHTLRNLMY LVSPSSEHLG RSGPFEVRAD
410 420 430 440 450
LEESMEFVDP GVAGESDESG DERVSDEETD LGTDWENLPS PRFCDIPSQS
460 470 480 490
VEVSQSQSTQ ASPPIASSSA GGMISSAAAS VTSWFKAYTG HR
Length:492
Mass (Da):55,309
Last modified:July 5, 2004 - v1
Checksum:iA3EAB0580077D7A6
GO
Isoform 2 (identifier: Q6ZNE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: Missing.

Show »
Length:379
Mass (Da):42,434
Checksum:iD6051FFD708A9943
GO

Sequence cautioni

The sequence BAA74854 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256N → S in AAI09090 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06124059V → I. Corresponds to variant dbSNP:rs57295720Ensembl.1
Natural variantiVAR_049514131N → K. Corresponds to variant dbSNP:rs17675076Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0139311 – 113Missing in isoform 2. 1 PublicationAdd BLAST113

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020638 mRNA Translation: BAA74854.2 Different initiation.
AK131251 mRNA Translation: BAD18430.1
AK292810 mRNA Translation: BAF85499.1
AL158801 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW80673.1
BC109089 mRNA Translation: AAI09090.1
BC109090 mRNA Translation: AAI09091.1
BR000826 mRNA Translation: FAA00433.1
CCDSiCCDS32087.1 [Q6ZNE5-1]
RefSeqiNP_055739.2, NM_014924.4 [Q6ZNE5-1]
XP_011534865.1, XM_011536563.2 [Q6ZNE5-2]
UniGeneiHs.414809

Genome annotation databases

EnsembliENST00000247178; ENSP00000247178; ENSG00000126775 [Q6ZNE5-1]
GeneIDi22863
KEGGihsa:22863
UCSCiuc001xbx.3 human [Q6ZNE5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020638 mRNA Translation: BAA74854.2 Different initiation.
AK131251 mRNA Translation: BAD18430.1
AK292810 mRNA Translation: BAF85499.1
AL158801 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW80673.1
BC109089 mRNA Translation: AAI09090.1
BC109090 mRNA Translation: AAI09091.1
BR000826 mRNA Translation: FAA00433.1
CCDSiCCDS32087.1 [Q6ZNE5-1]
RefSeqiNP_055739.2, NM_014924.4 [Q6ZNE5-1]
XP_011534865.1, XM_011536563.2 [Q6ZNE5-2]
UniGeneiHs.414809

3D structure databases

ProteinModelPortaliQ6ZNE5
SMRiQ6ZNE5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116531, 22 interactors
ComplexPortaliCPX-73 Phosphatidylinositol 3-kinase complex, class III, ATG14 variant
CORUMiQ6ZNE5
DIPiDIP-48651N
IntActiQ6ZNE5, 11 interactors
MINTiQ6ZNE5
STRINGi9606.ENSP00000247178

Protein family/group databases

TCDBi9.A.15.2.1 the autophagy-related phagophore-formation transporter (apt) family

PTM databases

iPTMnetiQ6ZNE5
PhosphoSitePlusiQ6ZNE5

Polymorphism and mutation databases

BioMutaiATG14
DMDMi67461020

Proteomic databases

EPDiQ6ZNE5
MaxQBiQ6ZNE5
PaxDbiQ6ZNE5
PeptideAtlasiQ6ZNE5
PRIDEiQ6ZNE5
ProteomicsDBi68016
68017 [Q6ZNE5-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247178; ENSP00000247178; ENSG00000126775 [Q6ZNE5-1]
GeneIDi22863
KEGGihsa:22863
UCSCiuc001xbx.3 human [Q6ZNE5-1]

Organism-specific databases

CTDi22863
DisGeNETi22863
EuPathDBiHostDB:ENSG00000126775.8
GeneCardsiATG14
HGNCiHGNC:19962 ATG14
HPAiCAB037242
MIMi613515 gene
neXtProtiNX_Q6ZNE5
OpenTargetsiENSG00000126775
PharmGKBiPA165478560
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4398 Eukaryota
ENOG410Y9W5 LUCA
GeneTreeiENSGT00390000011854
HOVERGENiHBG081828
InParanoidiQ6ZNE5
KOiK17889
OMAiNTSYIRG
OrthoDBiEOG091G074J
PhylomeDBiQ6ZNE5
TreeFamiTF323392

Enzyme and pathway databases

BRENDAi2.7.1.137 2681
ReactomeiR-HSA-1632852 Macroautophagy
SIGNORiQ6ZNE5

Miscellaneous databases

ChiTaRSiATG14 human
GenomeRNAii22863
PROiPR:Q6ZNE5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000126775 Expressed in 229 organ(s), highest expression level in female gonad
CleanExiHS_KIAA0831
GenevisibleiQ6ZNE5 HS

Family and domain databases

InterProiView protein in InterPro
IPR018791 UV_resistance/autophagy_Atg14
PfamiView protein in Pfam
PF10186 Atg14, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBAKOR_HUMAN
AccessioniPrimary (citable) accession number: Q6ZNE5
Secondary accession number(s): A6NJE4
, A8K9U5, B7ZWP5, O94920, Q32MK7, Q32MK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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