UniProtKB - Q6ZNE5 (BAKOR_HUMAN)
Beclin 1-associated autophagy-related key regulator
ATG14
Functioni
GO - Molecular functioni
- GTPase binding Source: UniProtKB
GO - Biological processi
- autophagosome assembly Source: UniProtKB
- autophagosome membrane docking Source: GO_Central
- cellular response to glucose starvation Source: UniProtKB
- cellular response to starvation Source: ParkinsonsUK-UCL
- endosome to lysosome transport Source: MGI
- macroautophagy Source: ParkinsonsUK-UCL
- mitophagy Source: ParkinsonsUK-UCL
- negative regulation of protein phosphorylation Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
- positive regulation of protein phosphorylation Source: UniProtKB
- posttranscriptional regulation of gene expression Source: ParkinsonsUK-UCL
- regulation of protein complex stability Source: ParkinsonsUK-UCL
- regulation of protein phosphorylation Source: ParkinsonsUK-UCL
- regulation of triglyceride metabolic process Source: Ensembl
- response to mitochondrial depolarisation Source: BHF-UCL
Keywordsi
Biological process | Autophagy |
Enzyme and pathway databases
BRENDAi | 2.7.1.137, 2681 |
PathwayCommonsi | Q6ZNE5 |
Reactomei | R-HSA-1632852, Macroautophagy |
SIGNORi | Q6ZNE5 |
Protein family/group databases
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Beclin 1-associated autophagy-related key regulator1 PublicationShort name: Barkor1 Publication Alternative name(s): Autophagy-related protein 14-like protein1 Publication Short name: Atg14L1 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000126775.8 |
HGNCi | HGNC:19962, ATG14 |
MIMi | 613515, gene |
neXtProti | NX_Q6ZNE5 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Peripheral membrane protein Curated
Other locations
- Cytoplasm 1 Publication
- Preautophagosomal structure membrane 4 Publications; Peripheral membrane protein Curated
- autophagosome membrane 2 Publications; Peripheral membrane protein Curated
Note: Cytosolic under nutrient-rich conditions (PubMed:19050071). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:19050071). Accumulates on highly curved PtdIns3P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).By similarity
Cytoskeleton
- axoneme Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
Other locations
- autophagosome Source: UniProtKB
- autophagosome membrane Source: UniProtKB-SubCell
- extrinsic component of omegasome membrane Source: UniProtKB
- extrinsic component of phagophore assembly site membrane Source: UniProtKB
- mitochondria-associated endoplasmic reticulum membrane Source: GO_Central
- phagocytic vesicle Source: Ensembl
- phagophore assembly site membrane Source: UniProtKB
- phosphatidylinositol 3-kinase complex, class III Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 43 | C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-46; A-55 and A-58. 2 Publications | 1 | |
Mutagenesisi | 46 | C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-43; A-55 and A-58. 2 Publications | 1 | |
Mutagenesisi | 55 | C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-43; A-46 and A-58. 2 Publications | 1 | |
Mutagenesisi | 58 | C → A in Atg14L4C4A; fails to localize to the endoplasmic reticulum; when associated with A-43; A-46 and A-55. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 22863 |
OpenTargetsi | ENSG00000126775 |
PharmGKBi | PA165478560 |
Miscellaneous databases
Pharosi | Q6ZNE5, Tbio |
Polymorphism and mutation databases
BioMutai | ATG14 |
DMDMi | 67461020 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050774 | 1 – 492 | Beclin 1-associated autophagy-related key regulatorAdd BLAST | 492 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 29 | PhosphoserineCombined sources | 1 | |
Modified residuei | 416 | PhosphoserineCombined sources | 1 | |
Modified residuei | 429 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6ZNE5 |
jPOSTi | Q6ZNE5 |
MassIVEi | Q6ZNE5 |
MaxQBi | Q6ZNE5 |
PaxDbi | Q6ZNE5 |
PeptideAtlasi | Q6ZNE5 |
PRIDEi | Q6ZNE5 |
ProteomicsDBi | 68016 [Q6ZNE5-1] 68017 [Q6ZNE5-2] |
PTM databases
iPTMneti | Q6ZNE5 |
PhosphoSitePlusi | Q6ZNE5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000126775, Expressed in female gonad and 241 other tissues |
Genevisiblei | Q6ZNE5, HS |
Organism-specific databases
HPAi | ENSG00000126775, Low tissue specificity |
Interactioni
Subunit structurei
Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion (PubMed:25686604).
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex I (PI3KC3-C1) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with ATG14 (PubMed:18843052, PubMed:19050071, PubMed:19270696, PubMed:22314358, PubMed:23878393). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). PI3KC3-C1 can associate with further regulatory subunits.
Interacts with PIK3CB (By similarity).
Interacts (via coiled-coil domain) with BECN2 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (PubMed:23954414, PubMed:28218432).
Interacts with the STX17-SNAP29 binary t-SNARE complex (PubMed:25686604).
Interacts with NRBF2 (By similarity).
Interacts with PIK3C3 and BECN1; this interaction is increased in the absence of TMEM39A (PubMed:31806350).
By similarityCurated10 PublicationsBinary interactionsi
Hide detailsQ6ZNE5
With | #Exp. | IntAct |
---|---|---|
BECN1 [Q14457] | 47 | EBI-2690371,EBI-949378 |
FSD2 [A1L4K1] | 3 | EBI-2690371,EBI-5661036 |
MIS18A [Q9NYP9] | 4 | EBI-2690371,EBI-1104552 |
PIK3C3 [Q8NEB9] | 26 | EBI-2690371,EBI-1056470 |
GO - Molecular functioni
- GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116531, 46 interactors |
ComplexPortali | CPX-73, Phosphatidylinositol 3-kinase complex, class III, ATG14 variant |
CORUMi | Q6ZNE5 |
DIPi | DIP-48651N |
IntActi | Q6ZNE5, 18 interactors |
MINTi | Q6ZNE5 |
STRINGi | 9606.ENSP00000247178 |
Miscellaneous databases
RNActi | Q6ZNE5, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q6ZNE5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 43 – 58 | Cysteine repeatsAdd BLAST | 16 | |
Regioni | 413 – 492 | BATSBy similarityAdd BLAST | 80 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 71 – 180 | Sequence analysisAdd BLAST | 110 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4398, Eukaryota |
GeneTreei | ENSGT00390000011854 |
HOGENOMi | CLU_046719_1_0_1 |
InParanoidi | Q6ZNE5 |
OMAi | QHRSNED |
OrthoDBi | 1332122at2759 |
PhylomeDBi | Q6ZNE5 |
TreeFami | TF323392 |
Family and domain databases
InterProi | View protein in InterPro IPR018791, UV_resistance/autophagy_Atg14 |
Pfami | View protein in Pfam PF10186, Atg14, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASPSGKGAR ALEAPGCGPR PLARDLVDSV DDAEGLYVAV ERCPLCNTTR
60 70 80 90 100
RRLTCAKCVQ SGDFVYFDGR DRERFIDKKE RLSRLKSKQE EFQKEVLKAM
110 120 130 140 150
EGKWITDQLR WKIMSCKMRI EQLKQTICKG NEEMEKNSEG LLKTKEKNQK
160 170 180 190 200
LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT IDLRSHYERL ANLRRSHILE
210 220 230 240 250
LTSVIFPIEE VKTGVRDPAD VSSESDSAMT SSTVSKLAEA RRTTYLSGRW
260 270 280 290 300
VCDDHNGDTS ISITGPWISL PNNGDYSAYY SWVEEKKTTQ GPDMEQSNPA
310 320 330 340 350
YTISAALCYA TQLVNILSHI LDVNLPKKLC NSEFCGENLS KQKFTRAVKK
360 370 380 390 400
LNANILYLCF SQHVNLDQLQ PLHTLRNLMY LVSPSSEHLG RSGPFEVRAD
410 420 430 440 450
LEESMEFVDP GVAGESDESG DERVSDEETD LGTDWENLPS PRFCDIPSQS
460 470 480 490
VEVSQSQSTQ ASPPIASSSA GGMISSAAAS VTSWFKAYTG HR
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 256 | N → S in AAI09090 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_061240 | 59 | V → I. Corresponds to variant dbSNP:rs57295720Ensembl. | 1 | |
Natural variantiVAR_049514 | 131 | N → K. Corresponds to variant dbSNP:rs17675076Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013931 | 1 – 113 | Missing in isoform 2. 1 PublicationAdd BLAST | 113 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB020638 mRNA Translation: BAA74854.2 Different initiation. AK131251 mRNA Translation: BAD18430.1 AK292810 mRNA Translation: BAF85499.1 AL158801 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW80673.1 BC109089 mRNA Translation: AAI09090.1 BC109090 mRNA Translation: AAI09091.1 BR000826 mRNA Translation: FAA00433.1 |
CCDSi | CCDS32087.1 [Q6ZNE5-1] |
RefSeqi | NP_055739.2, NM_014924.4 [Q6ZNE5-1] XP_011534865.1, XM_011536563.2 [Q6ZNE5-2] |
Genome annotation databases
Ensembli | ENST00000247178; ENSP00000247178; ENSG00000126775 [Q6ZNE5-1] |
GeneIDi | 22863 |
KEGGi | hsa:22863 |
UCSCi | uc001xbx.3, human [Q6ZNE5-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB020638 mRNA Translation: BAA74854.2 Different initiation. AK131251 mRNA Translation: BAD18430.1 AK292810 mRNA Translation: BAF85499.1 AL158801 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW80673.1 BC109089 mRNA Translation: AAI09090.1 BC109090 mRNA Translation: AAI09091.1 BR000826 mRNA Translation: FAA00433.1 |
CCDSi | CCDS32087.1 [Q6ZNE5-1] |
RefSeqi | NP_055739.2, NM_014924.4 [Q6ZNE5-1] XP_011534865.1, XM_011536563.2 [Q6ZNE5-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HOL | X-ray | 1.40 | C/D | 429-443 | [»] | |
SMRi | Q6ZNE5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116531, 46 interactors |
ComplexPortali | CPX-73, Phosphatidylinositol 3-kinase complex, class III, ATG14 variant |
CORUMi | Q6ZNE5 |
DIPi | DIP-48651N |
IntActi | Q6ZNE5, 18 interactors |
MINTi | Q6ZNE5 |
STRINGi | 9606.ENSP00000247178 |
Protein family/group databases
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
PTM databases
iPTMneti | Q6ZNE5 |
PhosphoSitePlusi | Q6ZNE5 |
Polymorphism and mutation databases
BioMutai | ATG14 |
DMDMi | 67461020 |
Proteomic databases
EPDi | Q6ZNE5 |
jPOSTi | Q6ZNE5 |
MassIVEi | Q6ZNE5 |
MaxQBi | Q6ZNE5 |
PaxDbi | Q6ZNE5 |
PeptideAtlasi | Q6ZNE5 |
PRIDEi | Q6ZNE5 |
ProteomicsDBi | 68016 [Q6ZNE5-1] 68017 [Q6ZNE5-2] |
Protocols and materials databases
Antibodypediai | 24031, 200 antibodies |
Genome annotation databases
Ensembli | ENST00000247178; ENSP00000247178; ENSG00000126775 [Q6ZNE5-1] |
GeneIDi | 22863 |
KEGGi | hsa:22863 |
UCSCi | uc001xbx.3, human [Q6ZNE5-1] |
Organism-specific databases
CTDi | 22863 |
DisGeNETi | 22863 |
EuPathDBi | HostDB:ENSG00000126775.8 |
GeneCardsi | ATG14 |
HGNCi | HGNC:19962, ATG14 |
HPAi | ENSG00000126775, Low tissue specificity |
MIMi | 613515, gene |
neXtProti | NX_Q6ZNE5 |
OpenTargetsi | ENSG00000126775 |
PharmGKBi | PA165478560 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4398, Eukaryota |
GeneTreei | ENSGT00390000011854 |
HOGENOMi | CLU_046719_1_0_1 |
InParanoidi | Q6ZNE5 |
OMAi | QHRSNED |
OrthoDBi | 1332122at2759 |
PhylomeDBi | Q6ZNE5 |
TreeFami | TF323392 |
Enzyme and pathway databases
BRENDAi | 2.7.1.137, 2681 |
PathwayCommonsi | Q6ZNE5 |
Reactomei | R-HSA-1632852, Macroautophagy |
SIGNORi | Q6ZNE5 |
Miscellaneous databases
BioGRID-ORCSi | 22863, 17 hits in 850 CRISPR screens |
ChiTaRSi | ATG14, human |
GenomeRNAii | 22863 |
Pharosi | Q6ZNE5, Tbio |
PROi | PR:Q6ZNE5 |
RNActi | Q6ZNE5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000126775, Expressed in female gonad and 241 other tissues |
Genevisiblei | Q6ZNE5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018791, UV_resistance/autophagy_Atg14 |
Pfami | View protein in Pfam PF10186, Atg14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAKOR_HUMAN | |
Accessioni | Q6ZNE5Primary (citable) accession number: Q6ZNE5 Secondary accession number(s): A6NJE4 Q32MK8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations