UniProtKB - Q6ZMZ0 (RN19B_HUMAN)
Protein
E3 ubiquitin-protein ligase RNF19B
Gene
RNF19B
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1 (PubMed:16709802, PubMed:27485036). Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells (PubMed:10438909). Protects against staurosporin-induced cell death (PubMed:27485036).3 Publications
Catalytic activityi
- [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.By similarity EC:2.3.2.31
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 119 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 122 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 142 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 145 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 206 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 211 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 228 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 233 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 238 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 241 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 246 | Zinc 3; via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 251 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 287 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 290 | Zinc 4PROSITE-ProRule annotation | 1 | |
Active sitei | 302 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 307 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 310 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 315 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 318 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 326 | Zinc 5; via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 333 | Zinc 5PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 119 – 168 | RING-type 1PROSITE-ProRule annotationAdd BLAST | 50 | |
Zinc fingeri | 186 – 251 | IBR-typePROSITE-ProRule annotationAdd BLAST | 66 | |
Zinc fingeri | 287 – 318 | RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin binding Source: UniProtKB
- ubiquitin conjugating enzyme binding Source: GO_Central
- ubiquitin protein ligase activity Source: UniProtKB
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- natural killer cell mediated cytotoxicity Source: Ensembl
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
- protein autoubiquitination Source: UniProtKB
- protein polyubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Adaptive immunity, Immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q6ZMZ0 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF19B (EC:2.3.2.31By similarity)Alternative name(s): IBR domain-containing protein 3 Natural killer lytic-associated molecule RING finger protein 19B |
Gene namesi | Name:RNF19B Synonyms:IBRDC3, NKLAM |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26886, RNF19B |
MIMi | 610872, gene |
neXtProti | NX_Q6ZMZ0 |
VEuPathDBi | HostDB:ENSG00000116514.16 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Curated
Other locations
- Cytoplasmic granule membrane 1 Publication; Multi-pass membrane protein 1 Publication
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Lysosome
- cytolytic granule Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
- ubiquitin ligase complex Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 354 – 374 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 415 – 435 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 127544 |
OpenTargetsi | ENSG00000116514 |
PharmGKBi | PA162401626 |
Miscellaneous databases
Pharosi | Q6ZMZ0, Tbio |
Genetic variation databases
BioMutai | RNF19B |
DMDMi | 160370005 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084129 | 1 – 732 | E3 ubiquitin-protein ligase RNF19BAdd BLAST | 732 |
Proteomic databases
MassIVEi | Q6ZMZ0 |
MaxQBi | Q6ZMZ0 |
PaxDbi | Q6ZMZ0 |
PeptideAtlasi | Q6ZMZ0 |
PRIDEi | Q6ZMZ0 |
ProteomicsDBi | 19093 33676 67939 [Q6ZMZ0-1] 67940 [Q6ZMZ0-2] 67941 [Q6ZMZ0-3] 67942 [Q6ZMZ0-4] |
PTM databases
iPTMneti | Q6ZMZ0 |
PhosphoSitePlusi | Q6ZMZ0 |
Expressioni
Tissue specificityi
Expressed specifically in natural killer cells, activated macrophages and cytotoxic T-cells (PubMed:10438909). Present in natural killer cells (at protein level) (PubMed:10912506). Ubiquitously expressed with high expression in testis (PubMed:27485036).3 Publications
Inductioni
In natural killer cells, by IFNB1/IFN-beta and IL2/interleukin-2 (at protein level) (PubMed:10438909). Up-regulated by endoplasmic reticulum (ER) stress triggered by thapsigargin or tunicamycin (PubMed:27485036).2 Publications
Gene expression databases
Bgeei | ENSG00000116514, Expressed in testis and 228 other tissues |
Genevisiblei | Q6ZMZ0, HS |
Organism-specific databases
HPAi | ENSG00000116514, Tissue enhanced (testis) |
Interactioni
Subunit structurei
Interacts with UBE2L3, UBE2L6 and UCKL1.
1 PublicationBinary interactionsi
Hide detailsQ6ZMZ0
GO - Molecular functioni
- ubiquitin binding Source: UniProtKB
- ubiquitin conjugating enzyme binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 126065, 83 interactors |
IntActi | Q6ZMZ0, 68 interactors |
STRINGi | 9606.ENSP00000362555 |
Miscellaneous databases
RNActi | Q6ZMZ0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 318 | Required for ubiquitin ligase activity and for protection against staurosporin-induced cell death1 PublicationAdd BLAST | 318 | |
Regioni | 115 – 337 | TRIAD supradomainPROSITE-ProRule annotationAdd BLAST | 223 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 32 | Poly-Arg | 4 | |
Compositional biasi | 41 – 97 | Ala-richAdd BLAST | 57 | |
Compositional biasi | 52 – 84 | Pro-richAdd BLAST | 33 | |
Compositional biasi | 625 – 630 | Poly-Gly | 6 |
Domaini
The first IBR-type zinc finger is the most crucial for interaction with UBE2L3, UBE2L6 and UCKL1.
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 119 – 168 | RING-type 1PROSITE-ProRule annotationAdd BLAST | 50 | |
Zinc fingeri | 186 – 251 | IBR-typePROSITE-ProRule annotationAdd BLAST | 66 | |
Zinc fingeri | 287 – 318 | RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1815, Eukaryota |
GeneTreei | ENSGT00940000158451 |
HOGENOMi | CLU_016793_3_0_1 |
InParanoidi | Q6ZMZ0 |
OMAi | LTSDECG |
OrthoDBi | 257621at2759 |
PhylomeDBi | Q6ZMZ0 |
TreeFami | TF324777 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR031127, E3_UB_ligase_RBR IPR002867, IBR_dom IPR044066, TRIAD_supradom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR11685, PTHR11685, 1 hit |
Pfami | View protein in Pfam PF01485, IBR, 2 hits |
SMARTi | View protein in SMART SM00647, IBR, 2 hits SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS51873, TRIAD, 1 hit PS50089, ZF_RING_2, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q6ZMZ0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSEKDSESP RSTSLHAAAP DPKCRSGGRR RRLTLHSVFS ASARGRRARA
60 70 80 90 100
KPQAEPPPPA AQPPPAPAPA AAQGPPPEAL PAEPAAEAEA EAAAAAAEPG
110 120 130 140 150
FDDEEAAEGG GPGAEEVECP LCLVRLPPER APRLLSCPHR SCRDCLRHYL
160 170 180 190 200
RLEISESRVP ISCPECSERL NPHDIRLLLA DPPLMHKYEE FMLRRYLASD
210 220 230 240 250
PDCRWCPAPD CGYAVIAYGC ASCPKLTCER EGCQTEFCYH CKQIWHPNQT
260 270 280 290 300
CDMARQQRAQ TLRVRTKHTS GLSYGQESGP ADDIKPCPRC SAYIIKMNDG
310 320 330 340 350
SCNHMTCAVC GCEFCWLCMK EISDLHYLSP SGCTFWGKKP WSRKKKILWQ
360 370 380 390 400
LGTLIGAPVG ISLIAGIAIP AMVIGIPVYV GRKIHSRYEG RKTSKHKRNL
410 420 430 440 450
AITGGVTLSV IASPVIAAVS VGIGVPIMLA YVYGVVPISL CRGGGCGVST
460 470 480 490 500
ANGKGVKIEF DEDDGPITVA DAWRALKNPS IGESSIEGLT SVLSTSGSPT
510 520 530 540 550
DGLSVMQGPY SETASFAALS GGTLSGGILS SGKGKYSRLE VQADVQKEIF
560 570 580 590 600
PKDTASLGAI SDNASTRAMA GSIISSYNPQ DRECNNMEIQ VDIEAKPSHY
610 620 630 640 650
QLVSGSSTED SLHVHAQMAE NEEEGSGGGG SEEDPPCRHQ SCEQKDCLAS
660 670 680 690 700
KPWDISLAQP ESIRSDLESS DAQSDDVPDI TSDECGSPRS HTAACPSTPR
710 720 730
AQGAPSPSAH MNLSALAEGQ TVLKPEGGEA RV
Sequence cautioni
The sequence AAI13561 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAI43688 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence EAX07490 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 284 | I → V in BAC11017 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028632 | 33 – 133 | Missing in isoform 3. 1 PublicationAdd BLAST | 101 | |
Alternative sequenceiVSP_028635 | 281 | Missing in isoform 2, isoform 3 and isoform 4. 3 Publications | 1 | |
Alternative sequenceiVSP_028633 | 583 – 588 | ECNNME → FSMIHA in isoform 2 and isoform 3. 3 Publications | 6 | |
Alternative sequenceiVSP_028634 | 589 – 732 | Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 144 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK074486 mRNA Translation: BAC11017.1 AK131439 mRNA Translation: BAD18585.1 AL031602 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07490.1 Different initiation. CH471059 Genomic DNA Translation: EAX07491.1 BC020595 mRNA Translation: AAH20595.2 BC062374 mRNA Translation: AAH62374.1 BC113560 mRNA Translation: AAI13561.1 Different initiation. BC143687 mRNA Translation: AAI43688.1 Different initiation. |
CCDSi | CCDS372.2 [Q6ZMZ0-1] CCDS44107.1 [Q6ZMZ0-2] CCDS72754.1 [Q6ZMZ0-4] |
RefSeqi | NP_001120833.1, NM_001127361.2 [Q6ZMZ0-2] NP_001287755.1, NM_001300826.1 [Q6ZMZ0-4] NP_699172.2, NM_153341.3 [Q6ZMZ0-1] XP_006710419.1, XM_006710356.2 [Q6ZMZ0-1] |
Genome annotation databases
Ensembli | ENST00000235150; ENSP00000235150; ENSG00000116514 [Q6ZMZ0-4] ENST00000356990; ENSP00000349482; ENSG00000116514 [Q6ZMZ0-2] ENST00000373456; ENSP00000362555; ENSG00000116514 [Q6ZMZ0-1] |
GeneIDi | 127544 |
KEGGi | hsa:127544 |
UCSCi | uc001bwm.5, human [Q6ZMZ0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK074486 mRNA Translation: BAC11017.1 AK131439 mRNA Translation: BAD18585.1 AL031602 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07490.1 Different initiation. CH471059 Genomic DNA Translation: EAX07491.1 BC020595 mRNA Translation: AAH20595.2 BC062374 mRNA Translation: AAH62374.1 BC113560 mRNA Translation: AAI13561.1 Different initiation. BC143687 mRNA Translation: AAI43688.1 Different initiation. |
CCDSi | CCDS372.2 [Q6ZMZ0-1] CCDS44107.1 [Q6ZMZ0-2] CCDS72754.1 [Q6ZMZ0-4] |
RefSeqi | NP_001120833.1, NM_001127361.2 [Q6ZMZ0-2] NP_001287755.1, NM_001300826.1 [Q6ZMZ0-4] NP_699172.2, NM_153341.3 [Q6ZMZ0-1] XP_006710419.1, XM_006710356.2 [Q6ZMZ0-1] |
3D structure databases
SMRi | Q6ZMZ0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 126065, 83 interactors |
IntActi | Q6ZMZ0, 68 interactors |
STRINGi | 9606.ENSP00000362555 |
PTM databases
iPTMneti | Q6ZMZ0 |
PhosphoSitePlusi | Q6ZMZ0 |
Genetic variation databases
BioMutai | RNF19B |
DMDMi | 160370005 |
Proteomic databases
MassIVEi | Q6ZMZ0 |
MaxQBi | Q6ZMZ0 |
PaxDbi | Q6ZMZ0 |
PeptideAtlasi | Q6ZMZ0 |
PRIDEi | Q6ZMZ0 |
ProteomicsDBi | 19093 33676 67939 [Q6ZMZ0-1] 67940 [Q6ZMZ0-2] 67941 [Q6ZMZ0-3] 67942 [Q6ZMZ0-4] |
Protocols and materials databases
Antibodypediai | 57831, 114 antibodies |
DNASUi | 127544 |
Genome annotation databases
Ensembli | ENST00000235150; ENSP00000235150; ENSG00000116514 [Q6ZMZ0-4] ENST00000356990; ENSP00000349482; ENSG00000116514 [Q6ZMZ0-2] ENST00000373456; ENSP00000362555; ENSG00000116514 [Q6ZMZ0-1] |
GeneIDi | 127544 |
KEGGi | hsa:127544 |
UCSCi | uc001bwm.5, human [Q6ZMZ0-1] |
Organism-specific databases
CTDi | 127544 |
DisGeNETi | 127544 |
GeneCardsi | RNF19B |
HGNCi | HGNC:26886, RNF19B |
HPAi | ENSG00000116514, Tissue enhanced (testis) |
MIMi | 610872, gene |
neXtProti | NX_Q6ZMZ0 |
OpenTargetsi | ENSG00000116514 |
PharmGKBi | PA162401626 |
VEuPathDBi | HostDB:ENSG00000116514.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1815, Eukaryota |
GeneTreei | ENSGT00940000158451 |
HOGENOMi | CLU_016793_3_0_1 |
InParanoidi | Q6ZMZ0 |
OMAi | LTSDECG |
OrthoDBi | 257621at2759 |
PhylomeDBi | Q6ZMZ0 |
TreeFami | TF324777 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q6ZMZ0 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 127544, 7 hits in 997 CRISPR screens |
ChiTaRSi | RNF19B, human |
GenomeRNAii | 127544 |
Pharosi | Q6ZMZ0, Tbio |
PROi | PR:Q6ZMZ0 |
RNActi | Q6ZMZ0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000116514, Expressed in testis and 228 other tissues |
Genevisiblei | Q6ZMZ0, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR031127, E3_UB_ligase_RBR IPR002867, IBR_dom IPR044066, TRIAD_supradom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR11685, PTHR11685, 1 hit |
Pfami | View protein in Pfam PF01485, IBR, 2 hits |
SMARTi | View protein in SMART SM00647, IBR, 2 hits SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS51873, TRIAD, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RN19B_HUMAN | |
Accessioni | Q6ZMZ0Primary (citable) accession number: Q6ZMZ0 Secondary accession number(s): B7ZLB2 Q8WUF3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | October 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families