UniProtKB - Q6YP21 (KAT3_HUMAN)
Protein
Kynurenine--oxoglutarate transaminase 3
Gene
KYAT3
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others. May catalyze the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond. Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro).By similarity
Caution
The first non-coding exon of CCBL2 is in common with that of RBMXL1.Curated
Catalytic activityi
- EC:2.6.1.7By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.6.1.63By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.6.1.63By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:4.4.1.13By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
pyridoxal 5'-phosphateBy similarity
: L-kynurenine degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes kynurenate from L-kynurenine.By similarityProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Kynurenine--oxoglutarate transaminase 3 (KYAT3), Kynurenine--oxoglutarate transaminase 1 (KYAT1), Cysteine-S-conjugate beta-lyase 2 (hCG_23341), Cysteine-S-conjugate beta-lyase 2 (KYAT3)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes kynurenate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 71 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 218 | SubstrateBy similarity | 1 | |
Binding sitei | 429 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- cysteine-S-conjugate beta-lyase activity Source: UniProtKB-EC
- kynurenine-glyoxylate transaminase activity Source: UniProtKB
- kynurenine-oxoglutarate transaminase activity Source: UniProtKB
- protein homodimerization activity Source: Ensembl
- pyridoxal phosphate binding Source: InterPro
- RNA binding Source: UniProtKB
GO - Biological processi
- 2-oxoglutarate metabolic process Source: Ensembl
- biosynthetic process Source: InterPro
- cellular amino acid metabolic process Source: UniProtKB
- kynurenine metabolic process Source: UniProtKB
- L-kynurenine catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aminotransferase, Lyase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MetaCyc:HS06422-MONOMER |
BRENDAi | 2.6.1.7, 2681 |
PathwayCommonsi | Q6YP21 |
Reactomei | R-HSA-71240, Tryptophan catabolism |
UniPathwayi | UPA00334;UER00726 |
Names & Taxonomyi
Protein namesi | Recommended name: Kynurenine--oxoglutarate transaminase 3By similarity (EC:2.6.1.7By similarity)Alternative name(s): |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:33238, KYAT3 |
MIMi | 610656, gene |
neXtProti | NX_Q6YP21 |
VEuPathDBi | HostDB:ENSG00000137944.16 |
Subcellular locationi
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 56267 |
OpenTargetsi | ENSG00000137944 |
PharmGKBi | PA162381274 |
Miscellaneous databases
Pharosi | Q6YP21, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2046260 |
DrugBanki | DB00114, Pyridoxal phosphate |
Genetic variation databases
BioMutai | KYAT3 |
DMDMi | 74710502 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
ChainiPRO_0000287704 | 1 – 454 | Kynurenine--oxoglutarate transaminase 3Add BLAST | 454 | ||
Isoform 3 (identifier: Q6YP21-3) | |||||
Initiator methioninei | RemovedCombined sources |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 116 | N6-acetyllysine; alternateCombined sources | 1 | ||
Modified residuei | 116 | N6-succinyllysine; alternateBy similarity | 1 | ||
Modified residuei | 280 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | ||
Isoform 3 (identifier: Q6YP21-3) | |||||
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q6YP21 |
jPOSTi | Q6YP21 |
MassIVEi | Q6YP21 |
MaxQBi | Q6YP21 |
PaxDbi | Q6YP21 |
PeptideAtlasi | Q6YP21 |
PRIDEi | Q6YP21 |
ProteomicsDBi | 67858 [Q6YP21-1] 67859 [Q6YP21-2] 67860 [Q6YP21-3] |
PTM databases
iPTMneti | Q6YP21 |
PhosphoSitePlusi | Q6YP21 |
Expressioni
Gene expression databases
Bgeei | ENSG00000137944, Expressed in parotid gland and 240 other tissues |
ExpressionAtlasi | Q6YP21, baseline and differential |
Genevisiblei | Q6YP21, HS |
Organism-specific databases
HPAi | ENSG00000137944, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 121128, 30 interactors |
IntActi | Q6YP21, 10 interactors |
STRINGi | 9606.ENSP00000260508 |
Chemistry databases
BindingDBi | Q6YP21 |
Miscellaneous databases
RNActi | Q6YP21, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0257, Eukaryota |
GeneTreei | ENSGT00940000155827 |
HOGENOMi | CLU_017584_4_0_1 |
InParanoidi | Q6YP21 |
OMAi | SVAMTGW |
PhylomeDBi | Q6YP21 |
TreeFami | TF352342 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR034612, KAT_III IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR43807:SF6, PTHR43807:SF6, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6YP21-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFLAQRSLCS LSGRAKFLKT ISSSKILGFS TSAKMSLKFT NAKRIEGLDS
60 70 80 90 100
NVWIEFTKLA ADPSVVNLGQ GFPDISPPTY VKEELSKIAA IDSLNQYTRG
110 120 130 140 150
FGHPSLVKAL SYLYEKLYQK QIDSNKEILV TVGAYGSLFN TIQALIDEGD
160 170 180 190 200
EVILIVPFYD CYEPMVRMAG ATPVFIPLRS KPVYGKRWSS SDWTLDPQEL
210 220 230 240 250
ESKFNSKTKA IILNTPHNPL GKVYNREELQ VIADLCIKYD TLCISDEVYE
260 270 280 290 300
WLVYSGNKHL KIATFPGMWE RTITIGSAGK TFSVTGWKLG WSIGPNHLIK
310 320 330 340 350
HLQTVQQNTI YTCATPLQEA LAQAFWIDIK RMDDPECYFN SLPKELEVKR
360 370 380 390 400
DRMVRLLESV GLKPIVPDGG YFIIADVSLL DPDLSDMKNN EPYDYKFVKW
410 420 430 440 450
MTKHKKLSAI PVSAFCNSET KSQFEKFVRF CFIKKDSTLD AAEEIIKAWS
VQKS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MRN7 | A0A0A0MRN7_HUMAN | Cysteine-S-conjugate beta-lyase 2 | KYAT3 | 262 | Annotation score: |
Sequence cautioni
The sequence AAC72959 differs from that shown. Reason: Frameshift.Curated
The sequence AAH00819 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAG29278 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 251 | W → R in CAG29278 (Ref. 7) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_032352 | 206 | S → P2 PublicationsCorresponds to variant dbSNP:rs1059370Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042841 | 1 – 34 | Missing in isoform 3. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_025603 | 152 – 167 | VILIV…EPMVR → YNACTVKFTNLKCTAG in isoform 2. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_025604 | 168 – 454 | Missing in isoform 2. 1 PublicationAdd BLAST | 287 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028624 mRNA Translation: AAK26163.1 CR627392 mRNA Translation: CAH10487.1 AK057176 mRNA Translation: BAG51875.1 AL139416 Genomic DNA No translation available. AL445991 Genomic DNA No translation available. BC000819 mRNA Translation: AAH00819.1 Different initiation. AF091090 mRNA Translation: AAC72959.1 Frameshift. CR450282 mRNA Translation: CAG29278.1 Frameshift. |
CCDSi | CCDS30766.1 [Q6YP21-1] CCDS30767.1 [Q6YP21-3] |
RefSeqi | NP_001008661.1, NM_001008661.2 [Q6YP21-1] NP_001008662.1, NM_001008662.2 [Q6YP21-3] |
Genome annotation databases
Ensembli | ENST00000260508; ENSP00000260508; ENSG00000137944 [Q6YP21-1] ENST00000370491; ENSP00000359522; ENSG00000137944 [Q6YP21-3] |
GeneIDi | 56267 |
KEGGi | hsa:56267 |
UCSCi | uc001dmp.3, human [Q6YP21-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028624 mRNA Translation: AAK26163.1 CR627392 mRNA Translation: CAH10487.1 AK057176 mRNA Translation: BAG51875.1 AL139416 Genomic DNA No translation available. AL445991 Genomic DNA No translation available. BC000819 mRNA Translation: AAH00819.1 Different initiation. AF091090 mRNA Translation: AAC72959.1 Frameshift. CR450282 mRNA Translation: CAG29278.1 Frameshift. |
CCDSi | CCDS30766.1 [Q6YP21-1] CCDS30767.1 [Q6YP21-3] |
RefSeqi | NP_001008661.1, NM_001008661.2 [Q6YP21-1] NP_001008662.1, NM_001008662.2 [Q6YP21-3] |
3D structure databases
SMRi | Q6YP21 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121128, 30 interactors |
IntActi | Q6YP21, 10 interactors |
STRINGi | 9606.ENSP00000260508 |
Chemistry databases
BindingDBi | Q6YP21 |
ChEMBLi | CHEMBL2046260 |
DrugBanki | DB00114, Pyridoxal phosphate |
PTM databases
iPTMneti | Q6YP21 |
PhosphoSitePlusi | Q6YP21 |
Genetic variation databases
BioMutai | KYAT3 |
DMDMi | 74710502 |
Proteomic databases
EPDi | Q6YP21 |
jPOSTi | Q6YP21 |
MassIVEi | Q6YP21 |
MaxQBi | Q6YP21 |
PaxDbi | Q6YP21 |
PeptideAtlasi | Q6YP21 |
PRIDEi | Q6YP21 |
ProteomicsDBi | 67858 [Q6YP21-1] 67859 [Q6YP21-2] 67860 [Q6YP21-3] |
Protocols and materials databases
Antibodypediai | 19822, 85 antibodies |
Genome annotation databases
Ensembli | ENST00000260508; ENSP00000260508; ENSG00000137944 [Q6YP21-1] ENST00000370491; ENSP00000359522; ENSG00000137944 [Q6YP21-3] |
GeneIDi | 56267 |
KEGGi | hsa:56267 |
UCSCi | uc001dmp.3, human [Q6YP21-1] |
Organism-specific databases
CTDi | 56267 |
DisGeNETi | 56267 |
GeneCardsi | KYAT3 |
HGNCi | HGNC:33238, KYAT3 |
HPAi | ENSG00000137944, Low tissue specificity |
MIMi | 610656, gene |
neXtProti | NX_Q6YP21 |
OpenTargetsi | ENSG00000137944 |
PharmGKBi | PA162381274 |
VEuPathDBi | HostDB:ENSG00000137944.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0257, Eukaryota |
GeneTreei | ENSGT00940000155827 |
HOGENOMi | CLU_017584_4_0_1 |
InParanoidi | Q6YP21 |
OMAi | SVAMTGW |
PhylomeDBi | Q6YP21 |
TreeFami | TF352342 |
Enzyme and pathway databases
UniPathwayi | UPA00334;UER00726 |
BioCyci | MetaCyc:HS06422-MONOMER |
BRENDAi | 2.6.1.7, 2681 |
PathwayCommonsi | Q6YP21 |
Reactomei | R-HSA-71240, Tryptophan catabolism |
Miscellaneous databases
BioGRID-ORCSi | 56267, 3 hits in 997 CRISPR screens |
ChiTaRSi | KYAT3, human |
GenomeRNAii | 56267 |
Pharosi | Q6YP21, Tbio |
PROi | PR:Q6YP21 |
RNActi | Q6YP21, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000137944, Expressed in parotid gland and 240 other tissues |
ExpressionAtlasi | Q6YP21, baseline and differential |
Genevisiblei | Q6YP21, HS |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR034612, KAT_III IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR43807:SF6, PTHR43807:SF6, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAT3_HUMAN | |
Accessioni | Q6YP21Primary (citable) accession number: Q6YP21 Secondary accession number(s): B3KQ13 Q9BVY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families