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UniProtKB - Q6XP49 (GLIS3_MOUSE)
Protein
Zinc finger protein GLIS3
Gene
Glis3
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 345 – 370 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 379 – 406 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 412 – 436 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 442 – 466 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 472 – 496 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription by RNA polymerase II Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein GLIS3Alternative name(s): GLI-similar 3 |
Gene namesi | Name:Glis3Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2444289, Glis3 |
VEuPathDBi | HostDB:ENSMUSG00000052942 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047212 | 1 – 780 | Zinc finger protein GLIS3Add BLAST | 780 |
Proteomic databases
MaxQBi | Q6XP49 |
PaxDbi | Q6XP49 |
PRIDEi | Q6XP49 |
ProteomicsDBi | 270992 [Q6XP49-1] 270993 [Q6XP49-2] |
PTM databases
iPTMneti | Q6XP49 |
PhosphoSitePlusi | Q6XP49 |
Expressioni
Tissue specificityi
In the embryo, expressed at high levels in the kidney and testis. In the adult, expressed at high levels in the kidney and uterus and at lower levels in the brain, lung, skeletal muscle and pancreas.1 Publication
Developmental stagei
In the embryo, expression is detected between days 6.5 and 14.5, particularly during neurulation. At embryonic days 11.5 to 12.5, expression is high in the interdigital regions destined to undergo apoptosis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000052942, Expressed in zygote and 213 other tissues |
ExpressionAtlasi | Q6XP49, baseline and differential |
Interactioni
Protein-protein interaction databases
BioGRIDi | 230466, 1 interactor |
CORUMi | Q6XP49 |
STRINGi | 10090.ENSMUSP00000124635 |
Miscellaneous databases
RNActi | Q6XP49, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 148 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 290 – 315 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 485 – 512 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 527 – 670 | DisorderedSequence analysisAdd BLAST | 144 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 490 – 506 | Bipartite nuclear localization signalSequence analysisAdd BLAST | 17 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 80 – 95 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 103 – 120 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 292 – 309 | Pro residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 589 – 606 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 641 – 670 | Polar residuesSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the GLI C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 345 – 370 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 379 – 406 | C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 412 – 436 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 442 – 466 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 472 – 496 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000156896 |
HOGENOMi | CLU_481988_0_0_1 |
InParanoidi | Q6XP49 |
Family and domain databases
InterProi | View protein in InterPro IPR043359, GLI-like IPR030405, GLIS3 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR45718, PTHR45718, 1 hit PTHR45718:SF1, PTHR45718:SF1, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 5 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6XP49-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMVQRLGPIS PPASQVSTAC KQISPSLPRA VNAANLNRPP SDTRSVILQE
60 70 80 90 100
SLVSTTLSLT ESQSALSVKQ EWSQSYRAFP SLSSSHSSQN GTDLGDLLSL
110 120 130 140 150
PPGTPVSGNS VSNSLPPYLF GMENSHSPYP SPRHSATRAH STRSKKRALS
160 170 180 190 200
LSPLSDGIGI DFNTIIRTSP TSLVAYINGP RASPANLSPQ SEVYGHFLGV
210 220 230 240 250
RGSCIPQSCA VASGQKGILV ASGGHTLPGY GEDGTLEYER MQQLEHGGLQ
260 270 280 290 300
PGPVNNMVLQ PGLPGQDGQT ANMLKTERLE EFPASALDLP SALPLPLPPP
310 320 330 340 350
QGPPPPYHAH PHLHHPELLP HTQSLSLAQT GLEEDGEMED SGGKHCCRWI
360 370 380 390 400
DCSALYDQQE ELVRHIEKVH IDQRKGEDFT CFWTGCPRRY KPFNARYKLL
410 420 430 440 450
IHMRVHSGEK PNKCTFEGCK KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ
460 470 480 490 500
KAFSNSSDRA KHQRTHLDTK PYACQIPGCT KRYTDPSSLR KHVKAHSSRE
510 520 530 540 550
QQARKKLRSS TELHPDLLTD CLAVQPLQPA TSPGDAADHT VGHSPGPGPG
560 570 580 590 600
PGPGAELYSA PIFASNHSTR SGTAAGAGPP PHPVSHPSPG HNVQGSPHNP
610 620 630 640 650
SSQLPPLTAV DAGAERFAPP TPSPHHISPG RVPAPPSLLQ RAQAPHSQQP
660 670 680 690 700
PGSLLKPYQP ETNSSFQPNG IHVHGFYGQL QTFCPPHYPD SQRTVPPSGS
710 720 730 740 750
CSMVPSFEDC LVPTSMGQAG FDVFHRAFST HSGITVYDLP SASSSLFGES
760 770 780
LRSGPEDPTF LQLSAVDRCP SQLSSVYTEG
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ0GE24 | Q0GE24_MOUSE | GLIS3 | Glis3 | 935 | Annotation score: | ||
E0CX93 | E0CX93_MOUSE | Zinc finger protein GLIS3 | Glis3 | 245 | Annotation score: | ||
E0CYJ3 | E0CYJ3_MOUSE | Zinc finger protein GLIS3 | Glis3 | 142 | Annotation score: |
Sequence cautioni
The sequence AAP59883 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 274 | L → P in BAC35623 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 295 | L → I in BAC35623 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012281 | 1 | M → MNGRSCGMNLHRTSRTPQGP GLLGGQHIPPIRAHAGTPCS SSCASTPSPSIGSLANSLHL KMSSGAGMAPQSNMAASPIH LPALSPRRQLLANGKPQFQV TPAGVMAAPHTIKPKQQEFG DPFSPNPEKGALGFGPQCKS IGKGSCNNLVVTSSPM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_012282 | 508 – 516 | RSSTELHPD → TNICASSHW in isoform 2. 1 Publication | 9 | |
Alternative sequenceiVSP_012283 | 517 – 780 | Missing in isoform 2. 1 PublicationAdd BLAST | 264 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY220846 mRNA Translation: AAP59883.1 Different initiation. AK054020 mRNA Translation: BAC35623.1 |
RefSeqi | XP_006527048.1, XM_006526985.3 [Q6XP49-1] |
Genome annotation databases
Ensembli | ENSMUST00000112612; ENSMUSP00000108231; ENSMUSG00000052942 [Q6XP49-2] |
GeneIDi | 226075 |
UCSCi | uc008hch.2, mouse [Q6XP49-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY220846 mRNA Translation: AAP59883.1 Different initiation. AK054020 mRNA Translation: BAC35623.1 |
RefSeqi | XP_006527048.1, XM_006526985.3 [Q6XP49-1] |
3D structure databases
SMRi | Q6XP49 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230466, 1 interactor |
CORUMi | Q6XP49 |
STRINGi | 10090.ENSMUSP00000124635 |
PTM databases
iPTMneti | Q6XP49 |
PhosphoSitePlusi | Q6XP49 |
Proteomic databases
MaxQBi | Q6XP49 |
PaxDbi | Q6XP49 |
PRIDEi | Q6XP49 |
ProteomicsDBi | 270992 [Q6XP49-1] 270993 [Q6XP49-2] |
Protocols and materials databases
Antibodypediai | 23977, 101 antibodies from 21 providers |
DNASUi | 226075 |
Genome annotation databases
Ensembli | ENSMUST00000112612; ENSMUSP00000108231; ENSMUSG00000052942 [Q6XP49-2] |
GeneIDi | 226075 |
UCSCi | uc008hch.2, mouse [Q6XP49-1] |
Organism-specific databases
CTDi | 169792 |
MGIi | MGI:2444289, Glis3 |
VEuPathDBi | HostDB:ENSMUSG00000052942 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000156896 |
HOGENOMi | CLU_481988_0_0_1 |
InParanoidi | Q6XP49 |
Miscellaneous databases
BioGRID-ORCSi | 226075, 5 hits in 50 CRISPR screens |
ChiTaRSi | Glis3, mouse |
PROi | PR:Q6XP49 |
RNActi | Q6XP49, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052942, Expressed in zygote and 213 other tissues |
ExpressionAtlasi | Q6XP49, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR043359, GLI-like IPR030405, GLIS3 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR45718, PTHR45718, 1 hit PTHR45718:SF1, PTHR45718:SF1, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 5 hits |
SUPFAMi | SSF57667, SSF57667, 3 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 4 hits PS50157, ZINC_FINGER_C2H2_2, 5 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GLIS3_MOUSE | |
Accessioni | Q6XP49Primary (citable) accession number: Q6XP49 Secondary accession number(s): Q8BI60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 23, 2022 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families