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UniProtKB - Q6XNP7 (HPI_HEVBR)
Protein
Protease inhibitor HPI
Gene
PI1
Organism
Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Status
Functioni
Inhibitor of serine proteases, strongly inhibits subtilisin A and weakly inhibits trypsin. Does not inhibit chymotrypsin, papain, pepsin, pronase E, protease type XIII and thermolysin. HPI-1 inhibits subtilisin A with an Ki of 0.21 nM. HPI-2a inhibits subtilisin A with an Ki of 0.08 nM. HPI-2b inhibits subtilisin A with an Ki of 0.1 nM.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 46 – 47 | Reactive bondBy similarity | 2 |
GO - Molecular functioni
- serine-type endopeptidase inhibitor activity Source: UniProtKB
GO - Biological processi
- response to wounding Source: InterPro
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Protein family/group databases
MEROPSi | I13.008 |
Names & Taxonomyi
Protein namesi | Recommended name: Protease inhibitor HPIAlternative name(s): HbPI1 Protease inhibitor 1 |
Gene namesi | Name:PI1Imported |
Organismi | Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis) |
Taxonomic identifieri | 3981 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Malpighiales › Euphorbiaceae › Crotonoideae › Micrandreae › Hevea |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000273520 | 2 – 70 | Protease inhibitor HPI1 PublicationAdd BLAST | 69 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 5 | S-glutathionyl cysteine; alternate1 Publication | 1 | |
Disulfide bondi | 5 | Interchain; alternate1 Publication |
Post-translational modificationi
Occurs in 3 forms that differ in the modification of Cys-5, HPI-1 forms a homodimer through a disulfide bond, HPI-2a is modified by glutathionylation, and HPI-2b is covalently modified by addition of an unidentified adduct but not by a disulfide linkage.1 Publication
Keywords - PTMi
Acetylation, Disulfide bond, GlutathionylationPTM databases
iPTMneti | Q6XNP7 |
Interactioni
Subunit structurei
Monomer and homodimer; disulfide-linked.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family. [View classification]Sequence analysis
Phylogenomic databases
OrthoDBi | 1582946at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR000864, Prot_inh_pot1 IPR036354, Prot_inh_pot1_sf |
PANTHERi | PTHR33091, PTHR33091, 1 hit |
Pfami | View protein in Pfam PF00280, potato_inhibit, 1 hit |
PRINTSi | PR00292, POTATOINHBTR |
SUPFAMi | SSF54654, SSF54654, 1 hit |
PROSITEi | View protein in PROSITE PS00285, POTATO_INHIBITOR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q6XNP7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASQCPVKNS WPELVGTNGD IAAGIIQTEN ANVKAIVVKE GLPITQDLNF
60 70
NRVRVFVDEN RVVTQVPAIG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 – 10 | NS → DA in AAP46156 (PubMed:15010614).Curated | 2 |
Mass spectrometryi
Molecular mass is 14894 Da. Determined by MALDI. HPI-1 homodimer.1 Publication
Molecular mass is 7758 Da. Determined by MALDI. HPI-2a.1 Publication
Molecular mass is 7566 Da. Determined by MALDI. HPI-2b.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY221985 mRNA Translation: AAP46156.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY221985 mRNA Translation: AAP46156.1 |
3D structure databases
SMRi | Q6XNP7 |
ModBasei | Search... |
Protein family/group databases
Allergomei | 11621, Hev b 15 8756, Hev b SPI |
MEROPSi | I13.008 |
PTM databases
iPTMneti | Q6XNP7 |
Phylogenomic databases
OrthoDBi | 1582946at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR000864, Prot_inh_pot1 IPR036354, Prot_inh_pot1_sf |
PANTHERi | PTHR33091, PTHR33091, 1 hit |
Pfami | View protein in Pfam PF00280, potato_inhibit, 1 hit |
PRINTSi | PR00292, POTATOINHBTR |
SUPFAMi | SSF54654, SSF54654, 1 hit |
PROSITEi | View protein in PROSITE PS00285, POTATO_INHIBITOR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HPI_HEVBR | |
Accessioni | Q6XNP7Primary (citable) accession number: Q6XNP7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 22, 2020 | |
This is version 57 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families