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Protein

Methionine aminopeptidase 2

Gene

MAP2

Organism
Encephalitozoon hellem (strain ATCC 50504) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation1 Publication

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Activity regulationi

Irreversibly inhibited by the fungal metabolite fumagillin and the fumagillin analog TNP470, antiangiogenic drugs.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109SubstrateUniRule annotation1
Metal bindingi130Divalent metal cation 1UniRule annotation1
Metal bindingi141Divalent metal cation 1UniRule annotation1
Metal bindingi141Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi210Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei218SubstrateUniRule annotation1
Metal bindingi243Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi339Divalent metal cation 1UniRule annotation1
Metal bindingi339Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Enzyme and pathway databases

BRENDAi3.4.11.18 8141

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2UniRule annotation
Short name:
MetAP 2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:MAP2UniRule annotation
Ordered Locus Names:EHEL_100750
OrganismiEncephalitozoon hellem (strain ATCC 50504) (Microsporidian parasite)
Taxonomic identifieri907965 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000010086 Componenti: Chromosome X

Organism-specific databases

EuPathDBiMicrosporidiaDB:EHEL_100750

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489861 – 358Methionine aminopeptidase 2Add BLAST358

Proteomic databases

PRIDEiQ6XMH6

Interactioni

Protein-protein interaction databases

STRINGi907965.XP_003888149.1

Structurei

3D structure databases

ProteinModelPortaliQ6XMH6
SMRiQ6XMH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2775 Eukaryota
COG0024 LUCA
KOiK01265
OrthoDBiEOG092C3NQP

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03175 MetAP_2_euk, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6XMH6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFILMNQAA ELPIEFLPRD GAYRKGRLLD SKNAEVENTT ESDILQDARR
60 70 80 90 100
AAEAHRRVRY KVQSIIKPGM TLLEIVKSIE DSTRILLSGE RNNGIGFPAG
110 120 130 140 150
MSMNSCAAHY SVNPGEKDII LTENDVLKID FGTHSNGRIM DSAFTIAFKE
160 170 180 190 200
EFEPLLMAAK EGTETGIRSL GIDARVCDIG RDINEVISSY EMEVDGKKWA
210 220 230 240 250
IRPVSDLHGH SISQFKIHGG ISIPAVNNRD PTRITGDTFY AVETFATTGE
260 270 280 290 300
GFINDRSPCS HFMINTHKSR KLYNKDLIKV YEFVRDSFGT LPFSPRHLDY
310 320 330 340 350
YNLVEGSALK SVNLLTMMGL FTPYPPLNDI DGSKVAQFEH TVYLSESGKE

ILTRGDDY
Length:358
Mass (Da):40,071
Last modified:July 5, 2004 - v1
Checksum:i1EF1C9B28E0E8C39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224694 Genomic DNA Translation: AAP51023.1
CP002723 Genomic DNA Translation: AFM99168.1
AY339780 Genomic DNA Translation: AAR04554.1
RefSeqiXP_003888149.1, XM_003888100.1

Genome annotation databases

EnsemblFungiiAFM99168; AFM99168; EHEL_100750
GeneIDi13466604
KEGGiehe:EHEL_100750

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224694 Genomic DNA Translation: AAP51023.1
CP002723 Genomic DNA Translation: AFM99168.1
AY339780 Genomic DNA Translation: AAR04554.1
RefSeqiXP_003888149.1, XM_003888100.1

3D structure databases

ProteinModelPortaliQ6XMH6
SMRiQ6XMH6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi907965.XP_003888149.1

Proteomic databases

PRIDEiQ6XMH6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAFM99168; AFM99168; EHEL_100750
GeneIDi13466604
KEGGiehe:EHEL_100750

Organism-specific databases

EuPathDBiMicrosporidiaDB:EHEL_100750

Phylogenomic databases

eggNOGiKOG2775 Eukaryota
COG0024 LUCA
KOiK01265
OrthoDBiEOG092C3NQP

Enzyme and pathway databases

BRENDAi3.4.11.18 8141

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03175 MetAP_2_euk, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAP2_ENCHA
AccessioniPrimary (citable) accession number: Q6XMH6
Secondary accession number(s): I6UP09, Q6VH16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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