UniProtKB - Q6X7J4 (WOX9_ARATH)
Protein
WUSCHEL-related homeobox 9
Gene
WOX9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Homeodomain transcription factor required for meristem growth and early development (PubMed:15753038). Promotes cell proliferation and prevents premature differentiation in meristematic tissues during postembryonic development (PubMed:15753038). Essential for maintaining tissue growth during embryogenesis (PubMed:17706632). May act by repressing TSS to promote meristematic proliferation (PubMed:21185286). Involved in the transcriptional activation of a subset of cytokinin response factors (PubMed:20110319). May act as a negative regulator of cytokinin signaling in the dark (PubMed:21057190).1 Publication4 Publications
Miscellaneous
The seedling growth arrest phenotype of the stip mutants likely resulted from a G2 cell cycle arrest of the meristematic tissue (PubMed:17706632). Exogenous sucrose in the growth medium reactivate the stip meristems by repressing TSS and activating the expression of cell cycle regulators, and thus promoting G2 to M transition in meristematic tissues (PubMed:21185286).2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 51 – 115 | Homeobox; WUS-typePROSITE-ProRule annotationAdd BLAST | 65 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: TAIR
- transcription regulatory region sequence-specific DNA binding Source: TAIR
GO - Biological processi
- embryo development ending in seed dormancy Source: TAIR
- positive regulation of cell population proliferation Source: TAIR
- regulation of meristem growth Source: TAIR
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: WUSCHEL-related homeobox 91 PublicationAlternative name(s): Protein STIMPY1 Publication |
Gene namesi | Name:WOX91 Publication Synonyms:STIP1 Publication Ordered Locus Names:At2g33880Imported ORF Names:T1B8.31Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G33880 |
TAIRi | locus:2057614, AT2G33880 |
Pathology & Biotechi
Disruption phenotypei
Embryonic lethality when homozygous (PubMed:17706632). Reduction in embryonic growth, failure in axillary shoot apical meristem and leaf-primordia initiation and growth, and failure in primary root growth and lateral root initiation when heterozygous (PubMed:15753038, PubMed:17706632). Wox8 and wox9 double mutants are embryo lethal, with embryos disrupted as early as the first cell division in the embryo proper (PubMed:17706632).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049376 | 1 – 378 | WUSCHEL-related homeobox 9Add BLAST | 378 |
Proteomic databases
PaxDbi | Q6X7J4 |
PRIDEi | Q6X7J4 |
ProteomicsDBi | 242678 |
Expressioni
Tissue specificityi
Expressed in the basal cell and later at the boundary between suspensor and proembryo (PubMed:14711878). Expressed at low levels in proliferating tissues post embryonically (PubMed:15753038). Detected in vegetative shoot apical meristem, leaf primordia, floral meristems, emerging floral organs, epidermal layer of the placenta and in the upper portion of the root meristematic zone (PubMed:15753038, PubMed:20110319).3 Publications
Developmental stagei
First detected in the basal daughter cell of the zygote (PubMed:14711878). Unlike WOX2 and WOX8, it is not expressed in the egg cell or the zygote (PubMed:14711878). During two subsequent rounds of transverse cell divisions, it is restricted to the hypophysis (PubMed:14711878). At the 8-cell stage, it expands into the central domain of the embryo, in addition to weakening in the hypophysis (PubMed:14711878). After protoderm formation, expression in the central embryo domain is restricted to the protodermal cells and also disappears from the hypophyseal cell (PubMed:14711878). In subsequent stages, a ring of expression remains at the presumptive boundary between root and hypocotyl, at about the same position as WOX2 expression in heart stage embryos (PubMed:14711878). In addition to its embryonic expression, it is expressed postembryonically in the epidermal cells of the placenta during gynoecium development, but not in the developing ovules (PubMed:14711878). The placental expression disappears soon after fertilization (PubMed:14711878). Expression dynamics require MP and BDL, but not GN activity (PubMed:14711878). Detected as early as the first zygotic division in both the apical and the basal daughter cells (PubMed:17706632). By late globular to early transition stage, the protein is evenly distributed through out the embryo and the suspensor (PubMed:17706632). The basal half of the embryo, especially cells in the protoderm layer, starts to show slightly higher levels of expression by early heart stage (PubMed:17706632).2 Publications
Inductioni
Up-regulated in the zygote after fertilization by the transcription factor WRKY2.1 Publication
Gene expression databases
ExpressionAtlasi | Q6X7J4, baseline and differential |
Genevisiblei | Q6X7J4, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 3305, 11 interactors |
IntActi | Q6X7J4, 10 interactors |
STRINGi | 3702.AT2G33880.1 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 138 – 152 | Ser-richAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the WUS homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | ENOG502QVSY, Eukaryota |
HOGENOMi | CLU_030463_1_0_1 |
InParanoidi | Q6X7J4 |
PhylomeDBi | Q6X7J4 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR044557, WOX8/9-like |
PANTHERi | PTHR47288, PTHR47288, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50071, HOMEOBOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6X7J4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASSNRHWPS MFKSKPHPHQ WQHDINSPLL PSASHRSSPF SSGCEVERSP
60 70 80 90 100
EPKPRWNPKP EQIRILEAIF NSGMVNPPRE EIRRIRAQLQ EYGQVGDANV
110 120 130 140 150
FYWFQNRKSR SKHKLRLLHN HSKHSLPQTQ PQPQPQPSAS SSSSSSSSSS
160 170 180 190 200
KSTKPRKSKN KNNTNLSLGG SQMMGMFPPE PAFLFPVSTV GGFEGITVSS
210 220 230 240 250
QLGFLSGDMI EQQKPAPTCT GLLLSEIMNG SVSYGTHHQQ HLSEKEVEEM
260 270 280 290 300
RMKMLQQPQT QICYATTNHQ IASYNNNNNN NNIMLHIPPT TSTATTITTS
310 320 330 340 350
HSLATVPSTS DQLQVQADAR IRVFINEMEL EVSSGPFNVR DAFGEEVVLI
360 370
NSAGQPIVTD EYGVALHPLQ HGASYYLI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A178VQA8 | A0A178VQA8_ARATH | Homeobox-3 | HB-3 homeobox-3, STIMPY, STIP, WOX9, WOX9A | 388 | Annotation score: |
Sequence cautioni
The sequence AAC69146 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAC43105 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY251401 mRNA Translation: AAP37139.1 U78721 Genomic DNA Translation: AAC69146.1 Sequence problems. CP002685 Genomic DNA Translation: AEC08902.1 AK118501 mRNA Translation: BAC43105.1 Sequence problems. BT005296 mRNA Translation: AAO63360.1 |
PIRi | G84750 |
RefSeqi | NP_180944.2, NM_128948.4 |
Genome annotation databases
EnsemblPlantsi | AT2G33880.1; AT2G33880.1; AT2G33880 |
GeneIDi | 817958 |
Gramenei | AT2G33880.1; AT2G33880.1; AT2G33880 |
KEGGi | ath:AT2G33880 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY251401 mRNA Translation: AAP37139.1 U78721 Genomic DNA Translation: AAC69146.1 Sequence problems. CP002685 Genomic DNA Translation: AEC08902.1 AK118501 mRNA Translation: BAC43105.1 Sequence problems. BT005296 mRNA Translation: AAO63360.1 |
PIRi | G84750 |
RefSeqi | NP_180944.2, NM_128948.4 |
3D structure databases
SMRi | Q6X7J4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 3305, 11 interactors |
IntActi | Q6X7J4, 10 interactors |
STRINGi | 3702.AT2G33880.1 |
Proteomic databases
PaxDbi | Q6X7J4 |
PRIDEi | Q6X7J4 |
ProteomicsDBi | 242678 |
Genome annotation databases
EnsemblPlantsi | AT2G33880.1; AT2G33880.1; AT2G33880 |
GeneIDi | 817958 |
Gramenei | AT2G33880.1; AT2G33880.1; AT2G33880 |
KEGGi | ath:AT2G33880 |
Organism-specific databases
Araporti | AT2G33880 |
TAIRi | locus:2057614, AT2G33880 |
Phylogenomic databases
eggNOGi | ENOG502QVSY, Eukaryota |
HOGENOMi | CLU_030463_1_0_1 |
InParanoidi | Q6X7J4 |
PhylomeDBi | Q6X7J4 |
Miscellaneous databases
PROi | PR:Q6X7J4 |
Gene expression databases
ExpressionAtlasi | Q6X7J4, baseline and differential |
Genevisiblei | Q6X7J4, AT |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR044557, WOX8/9-like |
PANTHERi | PTHR47288, PTHR47288, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WOX9_ARATH | |
Accessioni | Q6X7J4Primary (citable) accession number: Q6X7J4 Secondary accession number(s): O23662, Q8GX13 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | April 7, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families