UniProtKB - Q6X1B6 (Q6X1B6_9MONO)
RNA-directed RNA polymerase L
L
Functioni
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
ARBA annotationRNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
UniRule annotationCatalytic activityi
- a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + 2 S-adenosyl-L-homocysteineARBA annotationEC:2.1.1.375ARBA annotation
- a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + GDP + H+ = a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + diphosphateARBA annotationEC:2.7.7.88ARBA annotation
- EC:2.7.7.48UniRule annotation
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GTPase activity Source: RHEA
- mRNA (guanine-N7-)-methyltransferase activity Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
Keywordsi
Molecular function | HydrolaseARBA annotation, MethyltransferaseUniRule annotationARBA annotation, Multifunctional enzymeARBA annotation, Nucleotidyltransferase, RNA-directed RNA polymeraseUniRule annotation, Transferase |
Biological process | mRNA cappingUniRule annotationARBA annotation, mRNA processing, Viral RNA replicationUniRule annotation |
Ligand | ATP-bindingUniRule annotation, Nucleotide-binding, S-adenosyl-L-methionineUniRule annotationARBA annotation |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-directed RNA polymerase LUniRule annotationShort name: Protein LUniRule annotation Alternative name(s): Large structural proteinUniRule annotation ReplicaseUniRule annotation TranscriptaseUniRule annotation Including the following 5 domains: Alternative name(s): PRNTaseUniRule annotation mRNA (nucleoside-2'-O-)-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation) Short name: N1-2'-O-MTaseUniRule annotation mRNA (guanine-N(7)-)-methyltransferaseUniRule annotation Short name: G-N7-MTaseUniRule annotation |
Gene namesi | Name:LImported |
Organismi | Avian orthoavulavirus 1Imported |
Taxonomic identifieri | 2560319 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Haploviricotina › Monjiviricetes › Mononegavirales › Paramyxoviridae › Avulavirinae › Orthoavulavirus |
Subcellular locationi
- Cytoplasm ARBA annotation
- Virion UniRule annotation
- Host cytoplasm UniRule annotation
Keywords - Cellular componenti
Host cytoplasmUniRule annotationARBA annotation, VirionUniRule annotationARBA annotationStructurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 634 – 818 | RdRp catalyticInterPro annotationAdd BLAST | 185 | |
Domaini | 1745 – 1958 | Mononegavirus-type SAM-dependent 2'-O-MTaseInterPro annotationAdd BLAST | 214 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 257 – 277 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysisFamily and domain databases
InterProi | View protein in InterPro IPR024352, L_methyltrans_paramyxo IPR039736, L_poly_C IPR026890, Mononeg_mRNAcap IPR014023, Mononeg_RNA_pol_cat IPR025786, Mononega_L_MeTrfase IPR016269, RNA-dir_pol_paramyxovirus |
Pfami | View protein in Pfam PF12803, G-7-MTase, 1 hit PF14318, Mononeg_mRNAcap, 1 hit PF00946, Mononeg_RNA_pol, 1 hit |
PIRSFi | PIRSF000830, RNA_pol_ParamyxoV, 1 hit |
TIGRFAMsi | TIGR04198, paramyx_RNAcap, 1 hit |
PROSITEi | View protein in PROSITE PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit PS51590, SAM_MT_MNV_L, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MASSGPERAE HQIILPESHL SSPLVKHKLL YYWKLTGLPL PDECDFDHLI
60 70 80 90 100
VSRQWKKILE SAAPDTERMI KLGRAVHQTL NHNSKITGVL HPRCLEKLAS
110 120 130 140 150
IEVPDSTNKF RKIEKKIQIH NTRYGELFTR LCTNVEKKLL GSSWSNNVSR
160 170 180 190 200
SEEFNSIRTD PAFWFHSKWS KAKFGWAHFK QVPRHLIVAA RTRSAVTKFV
210 220 230 240 250
TLTHKLGQVF GTPELVMGNH TNENKSPCLT QELVLMYADM MEGRDMVNII
260 270 280 290 300
SSTATHLRSL SEKIDDILRL VDALAKDLGN QVYDVVALME GFAYGAVQLL
310 320 330 340 350
EPSGTFAGDF FAFNLQELKD TLIELLPNDV AELVTHAIAT VFSGLEQNQA
360 370 380 390 400
AEMLCLLRLW GHPLLESRIA AKAVRSQMCA PKMIDFDMIL QVLSFFKGTI
410 420 430 440 450
INGYRKKNSG VWPHVKMDTI YGKVIGQLHA DSAEISHDVM LREYKSLSAL
460 470 480 490 500
EFEPCIEYDP VTNLSMFLKD KAIAHPRDNW LASFRRNLLS EDQKRHIKEA
510 520 530 540 550
TSTNRLLIEF LESNDFDPYK EMEYLTTLEY LRDDNVAVSY SLKEKEVKVN
560 570 580 590 600
GRIFAKLTKK LRNCQVMAEG ILADQIAPFF QGNGVIQDSI SLTKSMLAMS
610 620 630 640 650
QLSFNSNKKR ITDCKERVSS NRNHDPKSKN RRRVATFITT DLQKYCLNWR
660 670 680 690 700
YQTVKLFAHA INQLMGLPHF FEWIHLRLMD TTMFVGDPFN PPSDPTDCDL
710 720 730 740 750
SRVPNDDIYI VSARGGIEGL CQKLWTMISI AAIQLAAARS HCRVACMVQG
760 770 780 790 800
DNQVIAVTRE VRSDDSPEMV WTQWLQASDN FFKEMIHVNH LNGHNLKDRE
810 820 830 840 850
TIRSDTFFLY SKRIFKDGAI LSQVLKNSSK LVLISGDLSE NTVMSCANIA
860 870 880 890 900
STIARLCENG LPKDFCYYLN YIMSCVQTYF DSEFSITHSS QPDSNQSWFE
910 920 930 940 950
DISFVHSYVL TPAQLGGLSN LQYSRLYTRN IGDPGTTAFA EVKRLEAVGL
960 970 980 990 1000
LSPSIMTNIL TRPPGNGDWA SLCNDPYSFN FETVASPNIV LKKHTQKVLF
1010 1020 1030 1040 1050
ETCSNPLLSG VHTEDNEAEE KALVEFLLNQ EVVHPRVAHA IMESSSVGRR
1060 1070 1080 1090 1100
KQIQGLVDTT NTVINIALTR RPLGIKRLMR IINYSSMHAM LFTDDVFLSN
1110 1120 1130 1140 1150
RPNHPLVSSN MCSLTLADYA RNRSWSPLTG GRKILGVSNP DTIEPVEGEI
1160 1170 1180 1190 1200
LSVSGGCKKC DSGDEQFTWF HLPSNIQLTD DTSKNPPMRV PYLGSKTQER
1210 1220 1230 1240 1250
RAASLAKIAH MSPHVKAALR ASSVLIWAYG DNEVNWTAAL KIARSRCNIS
1260 1270 1280 1290 1300
SEYLRLLSPL PTAGNLQHRL DDGITQMTFT PASLYRVSPY IHISNDSQRL
1310 1320 1330 1340 1350
FTEEGIKEGN VVYQQIMLLG LSLIESLFPM TTTKTYDEIT LHLHSKFSCC
1360 1370 1380 1390 1400
IREAPVAVPF ELLGLAPELR AVTSNKFMYD PSPVSERDFA RLDLAIFKSY
1410 1420 1430 1440 1450
ELNLESYPTI ELMNILSISS GKLIGQSVVS YDEDTSIKND AIIVYDNTRN
1460 1470 1480 1490 1500
WISEAQNSDV VRLFEYAALE VLLDCSYQLY YLRVRGLNNI VLYMSDLYKN
1510 1520 1530 1540 1550
MPGILLSNIA ATISHPIIHS RLNAVGLVNH DGSHQLADTD FIEMSAKLLV
1560 1570 1580 1590 1600
SCTRRVVSGL YAGNKYDLLF PSVLDDNLSE KMLQLISRLC CLYTVLFATT
1610 1620 1630 1640 1650
REIPKIRGLS AEEKCSVLTE YLLSDAVKPL LRSEQVSCIM SPNIITFPAN
1660 1670 1680 1690 1700
LYYMSRKSLN LIREREDRDA ILALLFPQEP LLEFRPVQDI GVRVKDPFTR
1710 1720 1730 1740 1750
QPAALLQELD LSAPARYDAF TLNEVRSEHT LPNPEEDYLV RYLFRGIGTA
1760 1770 1780 1790 1800
SSSWYKASHL LSVPEVRCAR YGNSLYLAEG SGAIMSLLEL HVPHETIYYN
1810 1820 1830 1840 1850
TLFSNEMNPP QRHFGPTPTQ FLNSVVYRNL QAEVPCKDGF IQEFRPLWRE
1860 1870 1880 1890 1900
NAEESDLTSD KAVGYITSAV PYRSVSLLHC DIEIPPGSNQ SLLDQLAINL
1910 1920 1930 1940 1950
SLIAMHSVRE GGVVIIKVLY AMGYYFHLLM NLFTPCSTKG YILSNGYACR
1960 1970 1980 1990 2000
GDMECYLIFV MGYLGGPTFV HEVVRMAKTL VQRHGTLLSK SDEITLTRLF
2010 2020 2030 2040 2050
TSQQHRVTDI LSSPLPRLMK FLRENIDAAL IEAGGQPVRP FCAESLVSTL
2060 2070 2080 2090 2100
TDMTQMTQII ASHIDTVIRS VIYMEAEGDL ADTVFLFTPY NLSTDGKKRT
2110 2120 2130 2140 2150
SLKQCTRQIL EVTILGLRVK DLNKVGDVIG LVLRGMVSLE DLIPLRTYLR
2160 2170 2180 2190 2200
RSTCPKYLKA VLGITKLKEM FTDTSLLYLT LAQQKFYMKT IGNAAKGYYS
NCDS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY262106 mRNA Translation: AAP86958.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY262106 mRNA Translation: AAP86958.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family and domain databases
InterProi | View protein in InterPro IPR024352, L_methyltrans_paramyxo IPR039736, L_poly_C IPR026890, Mononeg_mRNAcap IPR014023, Mononeg_RNA_pol_cat IPR025786, Mononega_L_MeTrfase IPR016269, RNA-dir_pol_paramyxovirus |
Pfami | View protein in Pfam PF12803, G-7-MTase, 1 hit PF14318, Mononeg_mRNAcap, 1 hit PF00946, Mononeg_RNA_pol, 1 hit |
PIRSFi | PIRSF000830, RNA_pol_ParamyxoV, 1 hit |
TIGRFAMsi | TIGR04198, paramyx_RNAcap, 1 hit |
PROSITEi | View protein in PROSITE PS50526, RDRP_SSRNA_NEG_NONSEG, 1 hit PS51590, SAM_MT_MNV_L, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | Q6X1B6_9MONO | |
Accessioni | Q6X1B6Primary (citable) accession number: Q6X1B6 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 5, 2004 |
Last sequence update: | July 5, 2004 | |
Last modified: | January 19, 2022 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |