UniProtKB - Q6VZZ7 (OPN5_MOUSE)
Protein
Opsin-5
Gene
Opn5
Organism
Mus musculus (Mouse)
Status
Functioni
G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (PubMed:22043319). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319, PubMed:31607531). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (PubMed:30936473). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (PubMed:26392540, PubMed:30240620). Acts as a circadian photoreceptor in the outer ear and vibrissal pads, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (PubMed:31607531). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (PubMed:30936473). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (PubMed:30936473).5 Publications
GO - Molecular functioni
- 11-cis retinal binding Source: MGI
- G protein-coupled photoreceptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: MGI
GO - Biological processi
- cellular response to light stimulus Source: UniProtKB
- cellular response to UV-A Source: Ensembl
- entrainment of circadian clock by photoperiod Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: GO_Central
- hyaloid vascular plexus regression Source: UniProtKB
- phototransduction Source: UniProtKB
- phototransduction, UV Source: UniProtKB
- protein-chromophore linkage Source: UniProtKB-KW
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer |
Biological process | Sensory transduction, Vision |
Ligand | Chromophore |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins |
Names & Taxonomyi
Protein namesi | Recommended name: Opsin-5Alternative name(s): G-protein coupled receptor 136 G-protein coupled receptor PGR12 Neuropsin |
Gene namesi | Name:Opn5 Synonyms:Gpr136, Pgr12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2662912, Opn5 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | ExtracellularSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 74 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 75 – 95 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 96 – 108 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 109 – 129 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 130 – 150 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 151 – 171 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 172 – 197 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 198 – 218 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 219 – 252 | CytoplasmicSequence analysisAdd BLAST | 34 | |
Transmembranei | 253 – 273 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 274 – 288 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 289 – 309 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 310 – 377 | CytoplasmicSequence analysisAdd BLAST | 68 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Ultrastructure of the retina is normal (PubMed:30936473, PubMed:30240620, PubMed:26392540). Newborn mice show normal hyaloid vessel numbers and normal vessel cellularity (PubMed:30936473). Retinas show normal expression patterns of rod and cone opsins including Opn4 (PubMed:26392540, PubMed:30240620). Decreased activated Vegfr2 in the hyaloid vessels and increased activated Akt1 at P5 (PubMed:30936473). Reduced levels of dopamine in the retina, however increased levels in the vitreous at P6 (PubMed:30936473). Reduced number of hyaloid blood vessels due to precocious regression, however no change in abundance of Vegfa or Flt1 at P8 (PubMed:30936473). Expression of tyrosine hydroxylase Th in retinal cell processes at P8, with increased expression in developed dopaminergic amacrine cells at P15 (PubMed:30936473). Loss of retinal and corneal circadian rhythm photoentrainment (PubMed:26392540, PubMed:30240620). Reduced UVA-induced phase-shift response and Fos expression in the suprachiasmatic nuclei (SCN) in the brain (PubMed:30240620). Abolishes retinaldehyde-dependent photoentrainment in the dermal tissues of the outer ear and vibrissal pad (PubMed:31607531). Loss of phase shifting activity in response to violet light and expression of circadian clock-genes in the skin of the outer ear during light-to-dark cycle, including Per1, Per2, Cry2, Dbp and Nr1d2 (PubMed:31607531). Pde6b and Opn5 double knockout mice also show loss of retinal ultrastructures and a more severe reduction in the rate of circadian photoentrainment, light-induced phase-shift response and Fos expression in the SCN (PubMed:30240620).4 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197818 | 1 – 377 | Opsin-5Add BLAST | 377 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 4 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 106 ↔ 183 | PROSITE-ProRule annotation | ||
Modified residuei | 296 | N6-(retinylidene)lysine | 1 | |
Lipidationi | 315 | S-palmitoyl cysteineSequence analysis | 1 | |
Lipidationi | 316 | S-palmitoyl cysteineSequence analysis | 1 |
Post-translational modificationi
It is uncertain whether Cys-315 or Cys-316 is palmitoylated.Curated
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | Q6VZZ7 |
PRIDEi | Q6VZZ7 |
PTM databases
GlyGeni | Q6VZZ7, 1 site |
PhosphoSitePlusi | Q6VZZ7 |
Expressioni
Tissue specificityi
Expressed in the brain (at protein level) (PubMed:14623103, PubMed:22043319). Weakly expressed in the skin and liver (at protein level) (PubMed:22043319). Abundantly expressed in striated muscle cells (PubMed:22043319). Expressed in Math7/Atok7-dependent retinal ganglion cells in the ganglion cell layer (at protein level) (PubMed:14623103, PubMed:22043319, PubMed:26392540, PubMed:30240620, PubMed:31607531). Additionally expressed in horizontal and amacrine cells in the inner nuclear layer of the retina (at protein level) (PubMed:22043319). Expressed around the base of hair follicles and in epidermal and sebaceous gland cells of the outer ear (at protein level) (PubMed:22043319, PubMed:31607531). Abundantly expressed in vibrissae hair follicles and weakly expressed in the vibrissae skin pad, dorsal back skin, and tail (PubMed:31607531).5 Publications
Developmental stagei
Expressed weakly in the inner retina at postnatal day 5 (P5), with expression becoming abundant in retinal ganglion cells at P8 (PubMed:30936473). Expressed throughout the retinal sublaminae layers, with abundant expression in the ganglion cell layer and nerve fiber layer at P12 (PubMed:30936473). Expressed in ganglion cells in the optic tracts, superior colliculus and lateral geniculate nucleus of the brain at P28 (PubMed:30936473). Expressed around the base of hair follicles in the dorsal ear skin, in the vibrissal nose pad, and weakly expressed below the epidermis in the vibrissal nose pad at P8 (PubMed:31607531).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000043972, Expressed in lens of camera-type eye and 10 other tissues |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000063542 |
Miscellaneous databases
RNActi | Q6VZZ7, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222330 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | Q6VZZ7 |
OMAi | QFSVVPT |
OrthoDBi | 704940at2759 |
PhylomeDBi | Q6VZZ7 |
TreeFami | TF324998 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR002962, Peropsin IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01244, PEROPSIN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q6VZZ7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALNHTALPQ DERLPHYLRD EDPFASKLSW EADLVAGFYL TIIGILSTFG
60 70 80 90 100
NGYVLYMSSR RKKKLRPAEI MTINLAVCDL GISVVGKPFT IISCFCHRWV
110 120 130 140 150
FGWFGCRWYG WAGFFFGCGS LITMTAVSLD RYLKICYLSY GVWLKRKHAY
160 170 180 190 200
ICLAVIWAYA SFWTTMPLVG LGDYAPEPFG TSCTLDWWLA QASGGGQVFI
210 220 230 240 250
LSILFFCLLL PTAVIVFSYA KIIAKVKSSS KEVAHFDSRI HSSHVLEVKL
260 270 280 290 300
TKVAMLICAG FLIAWIPYAV VSVWSAFGRP DSIPIQLSVV PTLLAKSAAM
310 320 330 340 350
YNPIIYQVID YRFACCQAGG LRGTKKKSLE DFRLHTVTAV RKSSAVLEIH
360 370
PESSSRFTSA HVMDGESHSN DGDCGKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 – 86 | Missing in AAP72135 (PubMed:14623098).Curated | 3 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014041 | 353 | S → V in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_014042 | 354 – 377 | Missing in isoform 2. 1 PublicationAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY288426 mRNA Translation: AAP72135.1 BC125611 mRNA Translation: AAI25612.1 BC132010 mRNA Translation: AAI32011.1 AY255592 mRNA Translation: AAO85104.1 AK039525 mRNA Translation: BAC30373.1 AY318865 mRNA Translation: AAR08201.1 BK001605 Genomic DNA Translation: DAA01972.1 |
CCDSi | CCDS37619.1 [Q6VZZ7-1] |
RefSeqi | NP_861418.2, NM_181753.4 [Q6VZZ7-1] |
Genome annotation databases
Ensembli | ENSMUST00000068355; ENSMUSP00000063542; ENSMUSG00000043972 [Q6VZZ7-1] |
GeneIDi | 353344 |
KEGGi | mmu:353344 |
UCSCi | uc008coq.1, mouse [Q6VZZ7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY288426 mRNA Translation: AAP72135.1 BC125611 mRNA Translation: AAI25612.1 BC132010 mRNA Translation: AAI32011.1 AY255592 mRNA Translation: AAO85104.1 AK039525 mRNA Translation: BAC30373.1 AY318865 mRNA Translation: AAR08201.1 BK001605 Genomic DNA Translation: DAA01972.1 |
CCDSi | CCDS37619.1 [Q6VZZ7-1] |
RefSeqi | NP_861418.2, NM_181753.4 [Q6VZZ7-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000063542 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q6VZZ7, 1 site |
PhosphoSitePlusi | Q6VZZ7 |
Proteomic databases
PaxDbi | Q6VZZ7 |
PRIDEi | Q6VZZ7 |
Protocols and materials databases
Antibodypediai | 16965, 220 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000068355; ENSMUSP00000063542; ENSMUSG00000043972 [Q6VZZ7-1] |
GeneIDi | 353344 |
KEGGi | mmu:353344 |
UCSCi | uc008coq.1, mouse [Q6VZZ7-1] |
Organism-specific databases
CTDi | 221391 |
MGIi | MGI:2662912, Opn5 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222330 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | Q6VZZ7 |
OMAi | QFSVVPT |
OrthoDBi | 704940at2759 |
PhylomeDBi | Q6VZZ7 |
TreeFami | TF324998 |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins |
Miscellaneous databases
BioGRID-ORCSi | 353344, 0 hits in 17 CRISPR screens |
ChiTaRSi | Opn5, mouse |
PROi | PR:Q6VZZ7 |
RNActi | Q6VZZ7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000043972, Expressed in lens of camera-type eye and 10 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR002962, Peropsin IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01244, PEROPSIN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPN5_MOUSE | |
Accessioni | Q6VZZ7Primary (citable) accession number: Q6VZZ7 Secondary accession number(s): Q059L5 Q8BYI2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries