UniProtKB - Q6VMQ6 (MCAF1_HUMAN)
Activating transcription factor 7-interacting protein 1
ATF7IP
Functioni
GO - Molecular functioni
- ATPase activity Source: Ensembl
- transcription corepressor activity Source: Ensembl
GO - Biological processi
- DNA methylation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of protein stability Source: UniProtKB
- regulation of RNA polymerase II transcription preinitiation complex assembly Source: UniProtKB
- viral process Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Host-virus interaction, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q6VMQ6 |
Reactomei | R-HSA-3214841, PKMTs methylate histone lysines |
Names & Taxonomyi
Protein namesi | Recommended name: Activating transcription factor 7-interacting protein 1CuratedAlternative name(s): ATF-interacting protein Short name: ATF-IP ATF7-interacting protein ATFa-associated modulator Short name: hAM MBD1-containing chromatin-associated factor 1 P621 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20092, ATF7IP |
MIMi | 613644, gene |
neXtProti | NX_Q6VMQ6 |
VEuPathDBi | HostDB:ENSG00000171681.12 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- transcription regulator complex Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 968 | D → A: Abolishes the interaction with SUMO. 1 Publication | 1 | |
Mutagenesisi | 969 | L → A: Abolishes the interaction with SUMO. 1 Publication | 1 | |
Mutagenesisi | 1224 | L → R: Abolishes interaction with MBD1 and subsequent transcriptional repression. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55729 |
OpenTargetsi | ENSG00000171681 |
Miscellaneous databases
Pharosi | Q6VMQ6, Tbio |
Genetic variation databases
BioMutai | ATF7IP |
DMDMi | 317373420 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281780 | 1 – 1270 | Activating transcription factor 7-interacting protein 1Add BLAST | 1270 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Cross-linki | 33 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 57 | PhosphoserineCombined sources | 1 | |
Modified residuei | 113 | PhosphoserineCombined sources | 1 | |
Modified residuei | 118 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 445 | PhosphoserineCombined sources | 1 | |
Modified residuei | 473 | PhosphoserineCombined sources | 1 | |
Modified residuei | 474 | PhosphoserineCombined sources | 1 | |
Modified residuei | 477 | PhosphoserineCombined sources | 1 | |
Modified residuei | 479 | PhosphoserineBy similarity | 1 | |
Modified residuei | 496 | PhosphoserineCombined sources | 1 | |
Cross-linki | 558 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 559 | PhosphoserineCombined sources | 1 | |
Modified residuei | 673 | PhosphoserineCombined sources | 1 | |
Modified residuei | 899 | PhosphoserineCombined sources | 1 | |
Cross-linki | 910 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 938 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q6VMQ6 |
jPOSTi | Q6VMQ6 |
MassIVEi | Q6VMQ6 |
MaxQBi | Q6VMQ6 |
PaxDbi | Q6VMQ6 |
PeptideAtlasi | Q6VMQ6 |
PRIDEi | Q6VMQ6 |
ProteomicsDBi | 24334 32440 67728 [Q6VMQ6-1] 67729 [Q6VMQ6-2] |
PTM databases
GlyGeni | Q6VMQ6, 3 sites, 1 O-linked glycan (3 sites) |
iPTMneti | Q6VMQ6 |
PhosphoSitePlusi | Q6VMQ6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000171681, Expressed in amniotic fluid and 246 other tissues |
ExpressionAtlasi | Q6VMQ6, baseline and differential |
Genevisiblei | Q6VMQ6, HS |
Organism-specific databases
HPAi | ENSG00000171681, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MBD1; the interaction is enhanced when MBD1 is sumoylated (PubMed:12665582, PubMed:16757475).
Interacts with SETDB1; the interaction protects SETDB1 from proteasomal degradation and is required to stimulate histone methyltransferase activity and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (PubMed:14536086, PubMed:15691849, PubMed:27732843).
Interacts with SUMO ubiquitin-like proteins (SUMO1, SUNO2 and SUMO3), with a preference for SUMO2 and SUMO3 (PubMed:16757475, PubMed:18842587).
Interacts with SP1, ATF7 and ZHX1 (PubMed:10976766, PubMed:12659632, PubMed:19106100).
Interacts with the general transcription machinery, including ERCC2, ERCC3, GTF2E1, GTF2E2 and POLR2A (PubMed:19106100).
9 Publications(Microbial infection) Interacts with Epstein-Barr virus BRLF1/Rta protein, leading to the regulation of host genes in Epstein-Barr virus-infected cells.
1 PublicationBinary interactionsi
Hide detailsQ6VMQ6
With | #Exp. | IntAct |
---|---|---|
DISC1 [Q9NRI5] | 3 | EBI-928732,EBI-529989 |
GTF2IRD1 [Q9UHL9] | 3 | EBI-928732,EBI-372530 |
Isoform 2 [Q6VMQ6-2]
With | #Exp. | IntAct |
---|---|---|
EHMT2 [Q96KQ7] | 3 | EBI-12070560,EBI-744366 |
Protein-protein interaction databases
BioGRIDi | 120849, 45 interactors |
CORUMi | Q6VMQ6 |
IntActi | Q6VMQ6, 71 interactors |
MINTi | Q6VMQ6 |
STRINGi | 9606.ENSP00000440440 |
Miscellaneous databases
RNActi | Q6VMQ6, protein |
Structurei
3D structure databases
SMRi | Q6VMQ6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q6VMQ6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1160 – 1270 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 111 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 562 – 817 | Interaction with SETDB1Add BLAST | 256 | |
Regioni | 965 – 975 | Interaction with SUMO1 PublicationAdd BLAST | 11 | |
Regioni | 1154 – 1270 | Interaction with MBD1Add BLAST | 117 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 617 – 665 | Sequence analysisAdd BLAST | 49 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 553 – 571 | Nuclear localization signalBy similarityAdd BLAST | 19 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 349 – 580 | Glu-richAdd BLAST | 232 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QSM2, Eukaryota |
GeneTreei | ENSGT00530000063707 |
HOGENOMi | CLU_009529_0_0_1 |
InParanoidi | Q6VMQ6 |
OMAi | QVGIPFK |
OrthoDBi | 324622at2759 |
PhylomeDBi | Q6VMQ6 |
TreeFami | TF329427 |
Family and domain databases
DisProti | DP01277 |
Gene3Di | 2.60.40.10, 1 hit |
IDEALi | IID00216 |
InterProi | View protein in InterPro IPR026085, ATF7-int IPR031870, ATF7IP_BD IPR003961, FN3_dom IPR036116, FN3_sf IPR013783, Ig-like_fold |
PANTHERi | PTHR23210, PTHR23210, 1 hit |
Pfami | View protein in Pfam PF16788, ATF7IP_BD, 1 hit PF16794, fn3_4, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 15 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSLEEPQKK VFKARKTMRV SDRQQLEAVY KVKEELLKTD VKLLNGNHEN
60 70 80 90 100
GDLDPTSPLE NMDYIKDKEE VNGIEEICFD PEGSKAEWKE TPCILSVNVK
110 120 130 140 150
NKQDDDLNCE PLSPHNITPE PVSKLPAEPV SGDPAPGDLD AGDPASGVLA
160 170 180 190 200
SGDSTSGDPT SSEPSSSDAA SGDATSGDAP SGDVSPGDAT SGDATADDLS
210 220 230 240 250
SGDPTSSDPI PGEPVPVEPI SGDCAADDIA SSEITSVDLA SGAPASTDPA
260 270 280 290 300
SDDLASGDLS SSELASDDLA TGELASDELT SESTFDRTFE PKSVPVCEPV
310 320 330 340 350
PEIDNIEPSS NKDDDFLEKN GADEKLEQIQ SKDSLDEKNK ADNNIDANEE
360 370 380 390 400
TLETDDTTIC SDRPPENEKK VEEDIITELA LGEDAISSSM EIDQGEKNED
410 420 430 440 450
ETSADLVETI NENVIEDNKS ENILENTDSM ETDEIIPILE KLAPSEDELT
460 470 480 490 500
CFSKTSLLPI DETNPDLEEK MESSFGSPSK QESSESLPKE AFLVLSDEED
510 520 530 540 550
ISGEKDESEV ISQNETCSPA EVESNEKDNK PEEEEQVIHE DDERPSEKNE
560 570 580 590 600
FSRRKRSKSE DMDNVQSKRR RYMEEEYEAE FQVKITAKGD INQKLQKVIQ
610 620 630 640 650
WLLEEKLCAL QCAVFDKTLA ELKTRVEKIE CNKRHKTVLT ELQAKIARLT
660 670 680 690 700
KRFEAAKEDL KKRHEHPPNP PVSPGKTVND VNSNNNMSYR NAGTVRQMLE
710 720 730 740 750
SKRNVSESAP PSFQTPVNTV SSTNLVTPPA VVSSQPKLQT PVTSGSLTAT
760 770 780 790 800
SVLPAPNTAT VVATTQVPSG NPQPTISLQP LPVILHVPVA VSSQPQLLQS
810 820 830 840 850
HPGTLVTNQP SGNVEFISVQ SPPTVSGLTK NPVSLPSLPN PTKPNNVPSV
860 870 880 890 900
PSPSIQRNPT ASAAPLGTTL AVQAVPTAHS IVQATRTSLP TVGPSGLYSP
910 920 930 940 950
STNRGPIQMK IPISAFSTSS AAEQNSNTTP RIENQTNKTI DASVSKKAAD
960 970 980 990 1000
STSQCGKATG SDSSGVIDLT MDDEESGASQ DPKKLNHTPV STMSSSQPVS
1010 1020 1030 1040 1050
RPLQPIQPAP PLQPSGVPTS GPSQTTIHLL PTAPTTVNVT HRPVTQVTTR
1060 1070 1080 1090 1100
LPVPRAPANH QVVYTTLPAP PAQAPLRGTV MQAPAVRQVN PQNSVTVRVP
1110 1120 1130 1140 1150
QTTTYVVNNG LTLGSTGPQL TVHHRPPQVH TEPPRPVHPA PLPEAPQPQR
1160 1170 1180 1190 1200
LPPEAASTSL PQKPHLKLAR VQSQNGIVLS WSVLEVDRSC ATVDSYHLYA
1210 1220 1230 1240 1250
YHEEPSATVP SQWKKIGEVK ALPLPMACTL TQFVSGSKYY FAVRAKDIYG
1260 1270
RFGPFCDPQS TDVISSTQSS
Computationally mapped potential isoform sequencesi
There are 15 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MV73 | A8MV73_HUMAN | Activating transcription factor 7-i... | ATF7IP | 515 | Annotation score: | ||
F5H502 | F5H502_HUMAN | Activating transcription factor 7-i... | ATF7IP | 176 | Annotation score: | ||
F5GZ10 | F5GZ10_HUMAN | Activating transcription factor 7-i... | ATF7IP | 169 | Annotation score: | ||
F5H1K9 | F5H1K9_HUMAN | Activating transcription factor 7-i... | ATF7IP | 176 | Annotation score: | ||
F5H2H9 | F5H2H9_HUMAN | Activating transcription factor 7-i... | ATF7IP | 168 | Annotation score: | ||
F5H8I0 | F5H8I0_HUMAN | Activating transcription factor 7-i... | ATF7IP | 132 | Annotation score: | ||
F8WE35 | F8WE35_HUMAN | Activating transcription factor 7-i... | ATF7IP | 125 | Annotation score: | ||
F5H2W9 | F5H2W9_HUMAN | Activating transcription factor 7-i... | ATF7IP | 152 | Annotation score: | ||
F5GYR7 | F5GYR7_HUMAN | Activating transcription factor 7-i... | ATF7IP | 96 | Annotation score: | ||
H0YFA3 | H0YFA3_HUMAN | Activating transcription factor 7-i... | ATF7IP | 115 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 457 | L → V in AAH37312 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1182 | S → G in AAQ92978 (PubMed:14536086).Curated | 1 | |
Sequence conflicti | 1182 | S → G in BAA91751 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031283 | 278 | E → K. Corresponds to variant dbSNP:rs2231908Ensembl. | 1 | |
Natural variantiVAR_031284 | 348 | N → I1 PublicationCorresponds to variant dbSNP:rs2231909Ensembl. | 1 | |
Natural variantiVAR_031285 | 530 | K → R3 PublicationsCorresponds to variant dbSNP:rs3213764Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055912 | 1 | M → MHQDQRFRM in isoform 3. Curated | 1 | |
Alternative sequenceiVSP_024035 | 520 | Missing in isoform 2 and isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_024038 | 1095 – 1106 | VTVRV…TTTYV → KRFFLYMAPRYM in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_024039 | 1107 – 1270 | Missing in isoform 2. 1 PublicationAdd BLAST | 164 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000261168; ENSP00000261168; ENSG00000171681 [Q6VMQ6-1] ENST00000536444; ENSP00000445955; ENSG00000171681 [Q6VMQ6-5] ENST00000540793; ENSP00000444589; ENSG00000171681 [Q6VMQ6-1] ENST00000543189; ENSP00000443179; ENSG00000171681 [Q6VMQ6-2] ENST00000544627; ENSP00000440440; ENSG00000171681 [Q6VMQ6-4] |
GeneIDi | 55729 |
KEGGi | hsa:55729 |
UCSCi | uc001rbv.3, human [Q6VMQ6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2RPQ | NMR | - | B | 938-981 | [»] | |
SMRi | Q6VMQ6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120849, 45 interactors |
CORUMi | Q6VMQ6 |
IntActi | Q6VMQ6, 71 interactors |
MINTi | Q6VMQ6 |
STRINGi | 9606.ENSP00000440440 |
PTM databases
GlyGeni | Q6VMQ6, 3 sites, 1 O-linked glycan (3 sites) |
iPTMneti | Q6VMQ6 |
PhosphoSitePlusi | Q6VMQ6 |
Genetic variation databases
BioMutai | ATF7IP |
DMDMi | 317373420 |
Proteomic databases
EPDi | Q6VMQ6 |
jPOSTi | Q6VMQ6 |
MassIVEi | Q6VMQ6 |
MaxQBi | Q6VMQ6 |
PaxDbi | Q6VMQ6 |
PeptideAtlasi | Q6VMQ6 |
PRIDEi | Q6VMQ6 |
ProteomicsDBi | 24334 32440 67728 [Q6VMQ6-1] 67729 [Q6VMQ6-2] |
Protocols and materials databases
Antibodypediai | 12020, 92 antibodies |
Genome annotation databases
Ensembli | ENST00000261168; ENSP00000261168; ENSG00000171681 [Q6VMQ6-1] ENST00000536444; ENSP00000445955; ENSG00000171681 [Q6VMQ6-5] ENST00000540793; ENSP00000444589; ENSG00000171681 [Q6VMQ6-1] ENST00000543189; ENSP00000443179; ENSG00000171681 [Q6VMQ6-2] ENST00000544627; ENSP00000440440; ENSG00000171681 [Q6VMQ6-4] |
GeneIDi | 55729 |
KEGGi | hsa:55729 |
UCSCi | uc001rbv.3, human [Q6VMQ6-1] |
Organism-specific databases
CTDi | 55729 |
DisGeNETi | 55729 |
GeneCardsi | ATF7IP |
HGNCi | HGNC:20092, ATF7IP |
HPAi | ENSG00000171681, Low tissue specificity |
MIMi | 613644, gene |
neXtProti | NX_Q6VMQ6 |
OpenTargetsi | ENSG00000171681 |
VEuPathDBi | HostDB:ENSG00000171681.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSM2, Eukaryota |
GeneTreei | ENSGT00530000063707 |
HOGENOMi | CLU_009529_0_0_1 |
InParanoidi | Q6VMQ6 |
OMAi | QVGIPFK |
OrthoDBi | 324622at2759 |
PhylomeDBi | Q6VMQ6 |
TreeFami | TF329427 |
Enzyme and pathway databases
PathwayCommonsi | Q6VMQ6 |
Reactomei | R-HSA-3214841, PKMTs methylate histone lysines |
Miscellaneous databases
BioGRID-ORCSi | 55729, 21 hits in 1008 CRISPR screens |
ChiTaRSi | ATF7IP, human |
EvolutionaryTracei | Q6VMQ6 |
GeneWikii | ATF7IP |
GenomeRNAii | 55729 |
Pharosi | Q6VMQ6, Tbio |
PROi | PR:Q6VMQ6 |
RNActi | Q6VMQ6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000171681, Expressed in amniotic fluid and 246 other tissues |
ExpressionAtlasi | Q6VMQ6, baseline and differential |
Genevisiblei | Q6VMQ6, HS |
Family and domain databases
DisProti | DP01277 |
Gene3Di | 2.60.40.10, 1 hit |
IDEALi | IID00216 |
InterProi | View protein in InterPro IPR026085, ATF7-int IPR031870, ATF7IP_BD IPR003961, FN3_dom IPR036116, FN3_sf IPR013783, Ig-like_fold |
PANTHERi | PTHR23210, PTHR23210, 1 hit |
Pfami | View protein in Pfam PF16788, ATF7IP_BD, 1 hit PF16794, fn3_4, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCAF1_HUMAN | |
Accessioni | Q6VMQ6Primary (citable) accession number: Q6VMQ6 Secondary accession number(s): F5GX74 Q9Y4X8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | January 11, 2011 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families