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Protein

Shutoff protein

Gene

L4

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Protein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers.UniRule annotation

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotation, RNA-bindingUniRule annotation, Translational shuntUniRule annotation
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of eukaryotic host translation factors by virusUniRule annotation, TransportUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Shutoff proteinUniRule annotation
Alternative name(s):
100 kDa proteinUniRule annotation
Short name:
p100KUniRule annotation
100K-chaperone proteinUniRule annotation
L4-100KUniRule annotation
Shutoff protein 100KUniRule annotation
Gene namesi
Name:L4UniRule annotationImported
OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)Imported
Taxonomic identifieri28285 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000156915 Componenti: Genome
  • UP000125273 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei365Phosphotyrosine; by hostUniRule annotation1
Modified residuei682Phosphotyrosine; by hostUniRule annotation1

Post-translational modificationi

Methylated. Asymmetric dimethylation by host PRMT1 of the Arg/Gly-rich region may regulate shutoff protein binding to hexon and promote the capsid assembly in the nucleus.UniRule annotation
Might be cleaved by the viral protease.UniRule annotation
Phosphorylated. Tyrosine phosphorylation enhances preferential binding to tripartite leader mRNAs and allows ribosome shunting.UniRule annotation

Keywords - PTMi

MethylationUniRule annotation, PhosphoproteinUniRule annotation

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.UniRule annotation

Keywords - Developmental stagei

Late proteinUniRule annotation

Interactioni

Subunit structurei

Monomer. Interacts with hexon protein; this interaction allows chaperoning and trimerization of hexon proteins. Interacts (via N-terminus) with host initiation factor EIF4G (via C-terminus). Interacts (via RRM domain) with viral mRNAs that contain the tripartite leader; this interaction allows ribosome shunting and expression of viral late mRNAs.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni280 – 345Binding to host EIF4GUniRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the adenoviridae shutoff protein family.UniRule annotation

Family and domain databases

HAMAPiMF_04060 ADV_SHUT, 1 hit
InterProiView protein in InterPro
IPR003381 L4
PfamiView protein in Pfam
PF02438 Adeno_100, 1 hit

Sequencei

Sequence statusi: Complete.

Q6VGU3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESVEKKDSL TAPSEFATTA STDAANAPTT FPVEAPPLEE EEVIIEQDPG
60 70 80 90 100
FVSEDDEDRS VPTEDKKQDQ DNAEANEEQV GRGDERHGDY LDVGDDVLLK
110 120 130 140 150
HLQRQCAIIC DALQERSDVP LAIADVSLAY ERHLFSPRVP PKRQENGTCE
160 170 180 190 200
PNPRLNFYPV FAVPEVLATY HIFFQNCKIP LSCRANRSRA DKQLALRQGA
210 220 230 240 250
VIPDIASLNE VPKIFEGLGR DEKRAANALQ QENSENESHS GVLVELEGDN
260 270 280 290 300
ARLAVLKRSI EVTHFAYPAL NLPPKVMSTV MSELIVRRAQ PLERDANLQE
310 320 330 340 350
QTEEGLPAVG DEQLARWLQT REPADLEERR KLMMAAVLVT VELECMQRFF
360 370 380 390 400
ADPEMQRKLE ETLHYTFRQG YVRQACKISN VELCNLVSYL GILHENRLGQ
410 420 430 440 450
NVLHSTLKGE ARRDYVRDCV YLFLCYTWQT AMGVWQQCLE ECNLKELQKL
460 470 480 490 500
LKQNLKDLWT AFNERSVAAH LADIIFPERL LKTLQQGLPD FTSQSMLQNF
510 520 530 540 550
RNFILERSGI LPATCCALPS DFVPIKYREC PPPLWGHCYL LQLANYLAYH
560 570 580 590 600
SDIMEDVSGD GLLECHCRCN LCTPHRSLVC NSQLLNESQI IGTFELQGPS
610 620 630 640 650
PDEKSAAPGL KLTPGLWTSA YLRKFVPEDY HAHEIRFYED QSRPPNAELT
660 670 680 690 700
ACVITQGHIL GQLQAINKAR QEFLLRKGRG VYLDPQSGEE LNPIPPPPQP
710 720 730 740 750
YQQQPRALAS QDGTQKEAAA AAATHGRGGI LGQSGRGGFG RGGGGHDGRL
760 770 780 790 800
GEPRRGSFRG RRGVRRNTVT LGRIPLAGAP EIGNRFQHGY NLRSSGAAGT

ARSPTQP
Length:807
Mass (Da):90,213
Last modified:July 5, 2004 - v1
Checksum:iCF17561EDA7CBADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY339865 Genomic DNA Translation: AAQ19301.1
AY601635 Genomic DNA Translation: AAW65517.1
RefSeqiAP_000214.1, AC_000008.1

Similar proteinsi

Entry informationi

Entry nameiQ6VGU3_ADE05
AccessioniPrimary (citable) accession number: Q6VGU3
Secondary accession number(s): Q2KS05
Entry historyiIntegrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: January 31, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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