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Protein

Protein sidekick-2

Gene

Sdk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adhesion molecule that promotes lamina-specific synaptic connections in the retina and is specifically required for the formation of neuronal circuits that detect motion (PubMed:26287463). Acts by promoting formation of synapses between two specific retinal cell types: the retinal ganglion cells W3B-RGCs and the excitatory amacrine cells VG3-ACs. Formation of synapses between these two cells plays a key role in detection of motion (PubMed:26287463). Promotes synaptic connectivity via homophilic interactions (PubMed:26287463).1 Publication

GO - Biological processi

  • camera-type eye photoreceptor cell differentiation Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • retina layer formation Source: UniProtKB
  • synapse assembly Source: UniProtKB

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-373756 SDK interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sidekick-21 Publication
Gene namesi
Name:Sdk21 PublicationImported
Synonyms:Kiaa15141 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2443847 Sdk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 1936ExtracellularSequence analysisAdd BLAST1912
Transmembranei1937 – 1957HelicalSequence analysisAdd BLAST21
Topological domaini1958 – 2176CytoplasmicSequence analysisAdd BLAST219

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile but show decreased synaptic connectivity between the retinal ganglion cells W3B-RGCs and the excitatory amacrine cells VG3-ACs.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2174 – 2176Missing : Abolishes localization to synapses and induces diffuse expression in photoreceptor cells. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000022697925 – 2176Protein sidekick-2Add BLAST2152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 95PROSITE-ProRule annotation
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi241 ↔ 288PROSITE-ProRule annotation
Disulfide bondi334 ↔ 384PROSITE-ProRule annotation
Disulfide bondi427 ↔ 479PROSITE-ProRule annotation
Disulfide bondi521 ↔ 573PROSITE-ProRule annotation
Glycosylationi747N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi940N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi952N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1592N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6V4S5
PeptideAtlasiQ6V4S5
PRIDEiQ6V4S5

PTM databases

iPTMnetiQ6V4S5
PhosphoSitePlusiQ6V4S5

Expressioni

Tissue specificityi

Expressed in retinal ganglion cells (RGCs) that form synapses in distinct inner plexiform layer (IPL) sublaminae. Specifically expressed in specific subsets of retinal ganglion cells (RGCs), named W3B-RGCs, that specifically respond when the timing of the movement of a small object differs from that of the background, but not when they coincide (at protein level). Also present in excitatory amacrine cell type called VG3-ACs, that provide strong and selective input W3B-RGCs (at protein level) (PubMed:26287463). Expressed at low levels in the glomeruli (PubMed:15213259).2 Publications

Developmental stagei

Highly expressed in many fetal tissues, inlcuding kidney but shows markedly lower expression in adult organs. Expression in kidney is high throughout development with maximal expression occurring near birth.1 Publication

Gene expression databases

BgeeiENSMUSG00000041592 Expressed in 194 organ(s), highest expression level in camera-type eye
CleanExiMM_SDK2
ExpressionAtlasiQ6V4S5 baseline and differential
GenevisibleiQ6V4S5 MM

Interactioni

Subunit structurei

Homodimer; mediates homophilic interactions to promote cell adhesion (PubMed:15703275, PubMed:26287463). Interacts (via PDZ-binding motif) with MAGI1, MAGI2, DLG2, DLG3 and DLG4 (PubMed:20219992).3 Publications

Structurei

Secondary structure

12176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ6V4S5
SMRiQ6V4S5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 112Ig-like C2-type 1Add BLAST83
Domaini117 – 204Ig-like C2-type 2Add BLAST88
Domaini219 – 298Ig-like C2-type 3Add BLAST80
Domaini312 – 402Ig-like C2-type 4Add BLAST91
Domaini406 – 495Ig-like C2-type 5Add BLAST90
Domaini500 – 589Ig-like C2-type 6Add BLAST90
Domaini596 – 692Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST97
Domaini697 – 793Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST97
Domaini798 – 897Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST100
Domaini901 – 995Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST95
Domaini999 – 1098Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST100
Domaini1103 – 1201Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST99
Domaini1206 – 1303Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST98
Domaini1307 – 1401Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST95
Domaini1406 – 1503Fibronectin type-III 9PROSITE-ProRule annotationAdd BLAST98
Domaini1508 – 1625Fibronectin type-III 10PROSITE-ProRule annotationAdd BLAST118
Domaini1630 – 1726Fibronectin type-III 11PROSITE-ProRule annotationAdd BLAST97
Domaini1730 – 1825Fibronectin type-III 12PROSITE-ProRule annotationAdd BLAST96
Domaini1828 – 1930Fibronectin type-III 13PROSITE-ProRule annotationAdd BLAST103

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2170 – 2176PDZ-binding1 Publication7

Domaini

the PDZ-binding motif mediates interaction with PDZ domain-containing proteins MAGI1, MAGI2, DLG2, DLG3 and DLG4 and is required for is required for synaptic localization in photoreceptors.1 Publication

Sequence similaritiesi

Belongs to the sidekick family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQJJ Eukaryota
ENOG410YMNG LUCA
GeneTreeiENSGT00910000144009
HOGENOMiHOG000231052
HOVERGENiHBG080083
InParanoidiQ6V4S5
KOiK16353
OrthoDBiEOG091G0607
PhylomeDBiQ6V4S5
TreeFamiTF316846

Family and domain databases

CDDicd00063 FN3, 13 hits
Gene3Di2.60.40.10, 19 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF00041 fn3, 13 hits
PF07679 I-set, 4 hits
SMARTiView protein in SMART
SM00060 FN3, 13 hits
SM00409 IG, 6 hits
SM00408 IGc2, 6 hits
SUPFAMiSSF48726 SSF48726, 5 hits
SSF49265 SSF49265, 7 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 13 hits
PS50835 IG_LIKE, 6 hits

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6V4S5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFSSMWRLPL WTLLALHRIH SAGAQDDVPP YFKTEPVRTQ VHLEGNRLVL
60 70 80 90 100
TCMAEGSWPL EFKWLHNNRE LTRFSLEYRY MITSLDRTHA GFYRCIVRNR
110 120 130 140 150
MGALLQRQTE VQVAYMGSFE EGEKRQSVNH GEAAVIRAPR ISSFPRPQVT
160 170 180 190 200
WFRDGRKIPP SSRIAITLEN TLVILSTVAP DAGRYYVQAV NDKNGDNKTS
210 220 230 240 250
QPITLAVENV GGPADPIAPT IIIPPKNTSV VAGTSEVTME CVANARPLIK
260 270 280 290 300
LHIVWKKDGA PLSSGISDYN RRLTIANPTV SDAGYYECEA MLRSSSVAPV
310 320 330 340 350
TRGAYLSVLE PPQFVREPER HITAEMEKVV DIPCRAKGVP PPSITWYKDA
360 370 380 390 400
ALVEVGKLTR FKQRSDGGLQ ISGLLPDDTG MLQCFAHNAA GEAQTSTYLA
410 420 430 440 450
VTSIAPNITR GPLDSTVIDG MSVVLACETS GAPRPAITWQ KGERILASGS
460 470 480 490 500
VQLPRFTLLE SGSLLISPTH ISDAGTYTCL ATNSRGVDEA SADLVVWART
510 520 530 540 550
RITKPPQDQS VIKGTQASMV CGVTHDPRVT VRYVWEKDGA TLAVETNPRI
560 570 580 590 600
RLDRNGSLHI SQTWSGDIGT YTCRVLSAGG NDSRNAHLRV RQLPHAPEHP
610 620 630 640 650
VATLSTVERR AINLTWAKPF DGNSPLMRYV LEMSENNAPW TILLASVDPE
660 670 680 690 700
ATSVMVKGLV PARSYQFRLC AVNDVGKGQF SKDTERVSLP EEPPTAPPQN
710 720 730 740 750
VIASGRTNQS IMIQWQPPPE SHQNGILKGY IIRYCLAGLP VGYQFKNITD
760 770 780 790 800
ADVNNLLLED LIIWTNYEIE VAAYNSAGLG VYSSKVTEWT LQGVPTVPPG
810 820 830 840 850
NVHAEATNST TIRFTWNAPS PQFINGINQG YKLIAWEPAQ EEEVTMVTAR
860 870 880 890 900
PNFQDSIHVG FVSGLKKFTE YFTSVLCFTT PGDGPRSSPQ LVRTHEDVPG
910 920 930 940 950
PVGHLSFNDI LDTSLKVSWQ EPGEKNGILT GYRISWEEYN RTNTRVTHYL
960 970 980 990 1000
PNVTLEYRVT GLTALTTYTI EVAAMTSKGQ GQVSASTISS GVPPELPGAP
1010 1020 1030 1040 1050
TNLGISNIGP RSVTLQFRPG YDGKTSISRW VVEAQVGVIG EGEEWLLIYQ
1060 1070 1080 1090 1100
LGNEPDARSM EVPDLNPFTY YSFRMRQVNI VGTSPPSQPS RKIQTLQAPP
1110 1120 1130 1140 1150
DIAPANVTLR TASETSLWLR WMPLPEMEYN GNPESVGYKI KYGRSDGHGK
1160 1170 1180 1190 1200
TLSHTVQDRV EREYTIEDLE EWTEYRVQVQ AFNAIGSGPW SQAVVGRTRE
1210 1220 1230 1240 1250
SVPSSGPTNV SALATTSSSM LVRWSEIPEA DRNGLVLGYK VRYKEKDSDS
1260 1270 1280 1290 1300
QPRFWLVEGN SSRSAQLTGL GKYVLYEVQV LAFTRIGDGS PSHPPILERT
1310 1320 1330 1340 1350
LDDVPGPPMG ILFPEVRTTS VRLIWQPPAA PNGIILAYQI THRLNATTAN
1360 1370 1380 1390 1400
TATVEVLAPS ARQYMATGLK PESVYLFRIT AQTRKGWGEA AEALVVTTEK
1410 1420 1430 1440 1450
RDRPQPPSKP VVQQEDVKAR SVLLSWEPGS DGLSPVRYYT IQTRELPSGR
1460 1470 1480 1490 1500
WALHSASVSH NASAFTVDRL KPFTSYKFRV KATNDIGDSE FSEESESLTT
1510 1520 1530 1540 1550
LQAAPDEAPT ILSVTPHTTT SVLIRWQPPS EDKINGILLG FRIRYRELLY
1560 1570 1580 1590 1600
DGLRGFTLRG INNPGAKWAE LTSLYSMRNL TRPSLTQYEL DNLSKHRRYE
1610 1620 1630 1640 1650
IRMSIYNAVG EGPLSPPQEV FVGEAVPTAA PQNVAIHSAT ATQLDVTWEP
1660 1670 1680 1690 1700
PPLDNQNGDI QGYKIYFWEV QRRNLTERVK TLFLAENSVK LKNLTGYTAY
1710 1720 1730 1740 1750
MVSVAAFNAA GDGPRSTPTR GQTQQAAPSA PGSVKFSELT TTSVNVSWDA
1760 1770 1780 1790 1800
PQFPNGPLEG YRLVYEPCTP VDGVSKIVTV DVKGNSPLWL KVKDLAEGMT
1810 1820 1830 1840 1850
YRFRIKAKTF TYGPEIEANI TTGPGEGAPG PPGVPIIVRY SSAIAIHWSS
1860 1870 1880 1890 1900
GDPGKGPITR YVIEARPSDE GLWDILIKDI PKEVTSYTFS MDILKPGVSY
1910 1920 1930 1940 1950
DFRVIAVNDY GFGTPSSPSQ SVPAQKASPF YEEWWFLVVI ALVGLIFILL
1960 1970 1980 1990 2000
LVFVLIIRGQ SKKYSKKTDS GGNTKSGALG HGEMLSLDES SFPALELNNR
2010 2020 2030 2040 2050
RLSVKNSFCR KNGLYTRSPP RPSPGSLHYS DEDVTKYNDL IPAESSSLTE
2060 2070 2080 2090 2100
KPSEISDSQG SDSEYEVDTN TQKAHSFVNH YISDPTYYNS WRRQQKGISR
2110 2120 2130 2140 2150
AQAYSYTESD SGEPDHVTVP NSNSTQQGSL FRPKASRTPT PQNPPNPQSQ
2160 2170
QSTLYRPPSS LAPGSRAPIA GFSSFV
Length:2,176
Mass (Da):239,902
Last modified:July 5, 2004 - v1
Checksum:iCAF12063E0A6EAD3
GO
Isoform 2 (identifier: Q6V4S5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1602: Missing.

Note: No experimental confirmation available.
Show »
Length:574
Mass (Da):62,688
Checksum:iE1361FB34886BAAE
GO
Isoform 3 (identifier: Q6V4S5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     534-542: VWEKDGATL → SMQSGKGRL
     543-2176: Missing.

Note: Due to intron retention. No experimental confirmation available.
Show »
Length:542
Mass (Da):59,495
Checksum:iAB43214E341C4198
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1ASR7B1ASR7_MOUSE
Protein sidekick-2
Sdk2
895Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0175261 – 1602Missing in isoform 2. 1 PublicationAdd BLAST1602
Alternative sequenceiVSP_017527534 – 542VWEKDGATL → SMQSGKGRL in isoform 3. 1 Publication9
Alternative sequenceiVSP_017528543 – 2176Missing in isoform 3. 1 PublicationAdd BLAST1634

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY351699 mRNA Translation: AAQ57661.1
AK161326 mRNA Translation: BAE36324.1
BC038036 mRNA Translation: AAH38036.1
AK129379 mRNA Translation: BAC98189.1
CCDSiCCDS25605.1 [Q6V4S5-1]
RefSeqiNP_766388.2, NM_172800.2 [Q6V4S5-1]
UniGeneiMm.130504
Mm.401394

Genome annotation databases

EnsembliENSMUST00000041627; ENSMUSP00000038972; ENSMUSG00000041592 [Q6V4S5-1]
GeneIDi237979
KEGGimmu:237979
UCSCiuc007mfd.1 mouse [Q6V4S5-2]
uc007mfe.1 mouse [Q6V4S5-1]
uc007mfg.1 mouse [Q6V4S5-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

Discerning change - Issue 176 of February 2016

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY351699 mRNA Translation: AAQ57661.1
AK161326 mRNA Translation: BAE36324.1
BC038036 mRNA Translation: AAH38036.1
AK129379 mRNA Translation: BAC98189.1
CCDSiCCDS25605.1 [Q6V4S5-1]
RefSeqiNP_766388.2, NM_172800.2 [Q6V4S5-1]
UniGeneiMm.130504
Mm.401394

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5K6XX-ray2.70A/B22-403[»]
5K6YX-ray3.20A/B22-403[»]
5K6ZX-ray2.70A/B22-211[»]
5K70X-ray2.70A/B/C/D22-403[»]
5XX0X-ray2.40A/B1-403[»]
ProteinModelPortaliQ6V4S5
SMRiQ6V4S5
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ6V4S5
PhosphoSitePlusiQ6V4S5

Proteomic databases

PaxDbiQ6V4S5
PeptideAtlasiQ6V4S5
PRIDEiQ6V4S5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041627; ENSMUSP00000038972; ENSMUSG00000041592 [Q6V4S5-1]
GeneIDi237979
KEGGimmu:237979
UCSCiuc007mfd.1 mouse [Q6V4S5-2]
uc007mfe.1 mouse [Q6V4S5-1]
uc007mfg.1 mouse [Q6V4S5-3]

Organism-specific databases

CTDi54549
MGIiMGI:2443847 Sdk2
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IQJJ Eukaryota
ENOG410YMNG LUCA
GeneTreeiENSGT00910000144009
HOGENOMiHOG000231052
HOVERGENiHBG080083
InParanoidiQ6V4S5
KOiK16353
OrthoDBiEOG091G0607
PhylomeDBiQ6V4S5
TreeFamiTF316846

Enzyme and pathway databases

ReactomeiR-MMU-373756 SDK interactions

Miscellaneous databases

PROiPR:Q6V4S5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041592 Expressed in 194 organ(s), highest expression level in camera-type eye
CleanExiMM_SDK2
ExpressionAtlasiQ6V4S5 baseline and differential
GenevisibleiQ6V4S5 MM

Family and domain databases

CDDicd00063 FN3, 13 hits
Gene3Di2.60.40.10, 19 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF00041 fn3, 13 hits
PF07679 I-set, 4 hits
SMARTiView protein in SMART
SM00060 FN3, 13 hits
SM00409 IG, 6 hits
SM00408 IGc2, 6 hits
SUPFAMiSSF48726 SSF48726, 5 hits
SSF49265 SSF49265, 7 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 13 hits
PS50835 IG_LIKE, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSDK2_MOUSE
AccessioniPrimary (citable) accession number: Q6V4S5
Secondary accession number(s): Q3TTK1, Q5U5W7, Q6ZPP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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