UniProtKB - Q6UXV4 (MIC27_HUMAN)
MICOS complex subunit MIC27
APOOL
Functioni
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979).
2 PublicationsGO - Biological processi
- cristae formation Source: GO_Central
- inner mitochondrial membrane organization Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q6UXV4 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-8949613, Cristae formation |
SignaLinki | Q6UXV4 |
Names & Taxonomyi
Protein namesi | Recommended name: MICOS complex subunit MIC27Alternative name(s): Apolipoprotein O-like Protein FAM121A |
Gene namesi | Name:APOOL Synonyms:CXorf33, FAM121A, MIC27 ORF Names:UNQ8193/PRO23204 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24009, APOOL |
MIMi | 300955, gene |
neXtProti | NX_Q6UXV4 |
VEuPathDBi | HostDB:ENSG00000155008 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications; Multi-pass membrane protein 1 Publication
- Mitochondrion 2 Publications
Extracellular region or secreted
- extracellular region Source: Reactome
Mitochondrion
- MIB complex Source: UniProtKB
- MICOS complex Source: UniProtKB
- mitochondrion Source: UniProtKB
- SAM complex Source: UniProtKB
Other locations
- platelet alpha granule lumen Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 110 | Mitochondrial intermembraneSequence analysisAdd BLAST | 83 | |
Transmembranei | 111 – 129 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 130 – 137 | Mitochondrial matrixSequence analysis | 8 | |
Transmembranei | 138 – 155 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 156 – 268 | Mitochondrial intermembraneSequence analysisAdd BLAST | 113 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000155008 |
PharmGKBi | PA162376732 |
Miscellaneous databases
Pharosi | Q6UXV4, Tbio |
Genetic variation databases
BioMutai | APOOL |
DMDMi | 74749432 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 27 | MitochondrionSequence analysisAdd BLAST | 27 | |
ChainiPRO_0000042052 | 28 – 268 | MICOS complex subunit MIC27Add BLAST | 241 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 204 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6UXV4 |
jPOSTi | Q6UXV4 |
MassIVEi | Q6UXV4 |
MaxQBi | Q6UXV4 |
PaxDbi | Q6UXV4 |
PeptideAtlasi | Q6UXV4 |
PRIDEi | Q6UXV4 |
ProteomicsDBi | 67669 |
TopDownProteomicsi | Q6UXV4 |
PTM databases
iPTMneti | Q6UXV4 |
MetOSitei | Q6UXV4 |
PhosphoSitePlusi | Q6UXV4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000155008, Expressed in myocardium and 239 other tissues |
ExpressionAtlasi | Q6UXV4, baseline and differential |
Genevisiblei | Q6UXV4, HS |
Organism-specific databases
HPAi | ENSG00000155008, Low tissue specificity |
Interactioni
Subunit structurei
Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and MICOS13/MIC13. This complex was also known under the names MINOS or MitOS complex (PubMed:25764979, PubMed:25781180, PubMed:23704930, PubMed:25764979, PubMed:25781180, PubMed:25997101).The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex.
Interacts with MICOS10/MIC10, IMMT/MIC60 and APOO/MIC23/MIC26 (PubMed:25764979, PubMed:25781180, PubMed:23704930, PubMed:25764979, PubMed:25781180, PubMed:25997101).
4 PublicationsProtein-protein interaction databases
BioGRIDi | 126558, 80 interactors |
ComplexPortali | CPX-6141, MICOS mitochondrial contact site and cristae organizing system complex |
CORUMi | Q6UXV4 |
DIPi | DIP-47309N |
IntActi | Q6UXV4, 46 interactors |
MINTi | Q6UXV4 |
STRINGi | 9606.ENSP00000362268 |
Miscellaneous databases
RNActi | Q6UXV4, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 187 – 268 | DisorderedSequence analysisAdd BLAST | 82 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 187 – 203 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 226 – 244 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 252 – 268 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4798, Eukaryota |
GeneTreei | ENSGT00530000063666 |
HOGENOMi | CLU_048383_0_0_1 |
InParanoidi | Q6UXV4 |
OrthoDBi | 1605767at2759 |
PhylomeDBi | Q6UXV4 |
TreeFami | TF315313 |
Family and domain databases
InterProi | View protein in InterPro IPR019166, MIC26/MIC27 IPR033182, MIC26/MIC27_animal |
PANTHERi | PTHR14564, PTHR14564, 1 hit |
Pfami | View protein in Pfam PF09769, ApoO, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAAIRMGKLT TMPAGLIYAS VSVHAAKQEE SKKQLVKPEQ LPIYTAPPLQ
60 70 80 90 100
SKYVEEQPGH LQMGFASIRT ATGCYIGWCK GVYVFVKNGI MDTVQFGKDA
110 120 130 140 150
YVYLKNPPRD FLPKMGVITV SGLAGLVSAR KGSKFKKITY PLGLATLGAT
160 170 180 190 200
VCYPVQSVII AKVTAKKVYA TSQQIFGAVK SLWTKSSKEE SLPKPKEKTK
210 220 230 240 250
LGSSSEIEVP AKTTHVLKHS VPLPTELSSE AKTKSESTSG ATQFMPDPKL
260
MDHGQSHPED IDMYSTRS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WYF7 | A0A087WYF7_HUMAN | MICOS complex subunit | APOOL | 268 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY358193 mRNA Translation: AAQ88560.1 Z83820 Genomic DNA No translation available. Z99571 Genomic DNA No translation available. BC107096 mRNA Translation: AAI07097.1 |
CCDSi | CCDS48138.1 |
RefSeqi | NP_940852.3, NM_198450.5 |
Genome annotation databases
Ensembli | ENST00000373173; ENSP00000362268; ENSG00000155008 |
GeneIDi | 139322 |
KEGGi | hsa:139322 |
MANE-Selecti | ENST00000373173.7; ENSP00000362268.2; NM_198450.6; NP_940852.3 |
UCSCi | uc004eem.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY358193 mRNA Translation: AAQ88560.1 Z83820 Genomic DNA No translation available. Z99571 Genomic DNA No translation available. BC107096 mRNA Translation: AAI07097.1 |
CCDSi | CCDS48138.1 |
RefSeqi | NP_940852.3, NM_198450.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 126558, 80 interactors |
ComplexPortali | CPX-6141, MICOS mitochondrial contact site and cristae organizing system complex |
CORUMi | Q6UXV4 |
DIPi | DIP-47309N |
IntActi | Q6UXV4, 46 interactors |
MINTi | Q6UXV4 |
STRINGi | 9606.ENSP00000362268 |
PTM databases
iPTMneti | Q6UXV4 |
MetOSitei | Q6UXV4 |
PhosphoSitePlusi | Q6UXV4 |
Genetic variation databases
BioMutai | APOOL |
DMDMi | 74749432 |
Proteomic databases
EPDi | Q6UXV4 |
jPOSTi | Q6UXV4 |
MassIVEi | Q6UXV4 |
MaxQBi | Q6UXV4 |
PaxDbi | Q6UXV4 |
PeptideAtlasi | Q6UXV4 |
PRIDEi | Q6UXV4 |
ProteomicsDBi | 67669 |
TopDownProteomicsi | Q6UXV4 |
Protocols and materials databases
Antibodypediai | 390, 142 antibodies from 24 providers |
DNASUi | 139322 |
Genome annotation databases
Ensembli | ENST00000373173; ENSP00000362268; ENSG00000155008 |
GeneIDi | 139322 |
KEGGi | hsa:139322 |
MANE-Selecti | ENST00000373173.7; ENSP00000362268.2; NM_198450.6; NP_940852.3 |
UCSCi | uc004eem.4, human |
Organism-specific databases
CTDi | 139322 |
GeneCardsi | APOOL |
HGNCi | HGNC:24009, APOOL |
HPAi | ENSG00000155008, Low tissue specificity |
MIMi | 300955, gene |
neXtProti | NX_Q6UXV4 |
OpenTargetsi | ENSG00000155008 |
PharmGKBi | PA162376732 |
VEuPathDBi | HostDB:ENSG00000155008 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4798, Eukaryota |
GeneTreei | ENSGT00530000063666 |
HOGENOMi | CLU_048383_0_0_1 |
InParanoidi | Q6UXV4 |
OrthoDBi | 1605767at2759 |
PhylomeDBi | Q6UXV4 |
TreeFami | TF315313 |
Enzyme and pathway databases
PathwayCommonsi | Q6UXV4 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-8949613, Cristae formation |
SignaLinki | Q6UXV4 |
Miscellaneous databases
BioGRID-ORCSi | 139322, 23 hits in 670 CRISPR screens |
ChiTaRSi | APOOL, human |
GenomeRNAii | 139322 |
Pharosi | Q6UXV4, Tbio |
PROi | PR:Q6UXV4 |
RNActi | Q6UXV4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000155008, Expressed in myocardium and 239 other tissues |
ExpressionAtlasi | Q6UXV4, baseline and differential |
Genevisiblei | Q6UXV4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR019166, MIC26/MIC27 IPR033182, MIC26/MIC27_animal |
PANTHERi | PTHR14564, PTHR14564, 1 hit |
Pfami | View protein in Pfam PF09769, ApoO, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MIC27_HUMAN | |
Accessioni | Q6UXV4Primary (citable) accession number: Q6UXV4 Secondary accession number(s): Q3KNU7, Q5H9D1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | February 23, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families