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UniProtKB - Q6USK1 (GERS_OCIBA)
Protein
Geraniol synthase, chloroplastic
Gene
GES
Organism
Ocimum basilicum (Sweet basil)
Status
Functioni
Monoterpene synthase that catalyzes the formation of geraniol from geranyl diphosphate.
1 PublicationMiscellaneous
Exclusively observed in the geraniol chemotype of this organism.
Catalytic activityi
- EC:3.1.7.111 Publication
Cofactori
Mn2+1 PublicationNote: Binds 3 Mn2+ ions per subunit.1 Publication
Kineticsi
kcat is 0.8 sec(-1) with geranyl diphosphate as substrate.1 Publication
- KM=21 µM for geranyl diphosphate1 Publication
- KM=51 µM for Mn2+1 Publication
- Vmax=6.244 pmol/sec/mg enzyme1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
: terpenoid biosynthesis Pathwayi
This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 323 | Manganese 1By similarity | 1 | |
Metal bindingi | 323 | Manganese 2By similarity | 1 | |
Metal bindingi | 327 | Manganese 1By similarity | 1 | |
Metal bindingi | 327 | Manganese 2By similarity | 1 | |
Metal bindingi | 469 | Manganese 3By similarity | 1 | |
Metal bindingi | 473 | Manganese 3By similarity | 1 | |
Metal bindingi | 477 | Manganese 3By similarity | 1 |
GO - Molecular functioni
- hydrolase activity Source: UniProtKB
- magnesium ion binding Source: InterPro
- manganese ion binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- terpene synthase activity Source: InterPro
GO - Biological processi
- diterpenoid biosynthetic process Source: InterPro
- geranyl diphosphate metabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.7.11, 4385 |
SABIO-RKi | Q6USK1 |
UniPathwayi | UPA00213 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GES |
Organismi | Ocimum basilicum (Sweet basil) |
Taxonomic identifieri | 39350 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Lamiales › Lamiaceae › Nepetoideae › Ocimeae › Ociminae › Ocimum |
Subcellular locationi
Chloroplast
- chloroplast Curated
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 63 | ChloroplastSequence analysisAdd BLAST | 63 | |
ChainiPRO_0000418928 | 64 – 567 | Geraniol synthase, chloroplasticAdd BLAST | 504 |
Expressioni
Tissue specificityi
Expressed in the peltate glandular trichomes of the leaves.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 48 – 75 | DisorderedSequence analysisAdd BLAST | 28 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 323 – 327 | DDXXD motif | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 60 – 75 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Domaini
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similaritiesi
Keywords - Domaini
Transit peptideFamily and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q6USK1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSCARITVTL PYRSAKTSIQ RGITHYPALI RPRFSACTPL ASAMPLSSTP
60 70 80 90 100
LINGDNSQRK NTRQHMEESS SKRREYLLEE TTRKLQRNDT ESVEKLKLID
110 120 130 140 150
NIQQLGIGYY FEDAINAVLR SPFSTGEEDL FTAALRFRLL RHNGIEISPE
160 170 180 190 200
IFLKFKDERG KFDESDTLGL LSLYEASNLG VAGEEILEEA MEFAEARLRR
210 220 230 240 250
SLSEPAAPLH GEVAQALDVP RHLRMARLEA RRFIEQYGKQ SDHDGDLLEL
260 270 280 290 300
AILDYNQVQA QHQSELTEII RWWKELGLVD KLSFGRDRPL ECFLWTVGLL
310 320 330 340 350
PEPKYSSVRI ELAKAISILL VIDDIFDTYG EMDDLILFTD AIRRWDLEAM
360 370 380 390 400
EGLPEYMKIC YMALYNTTNE VCYKVLRDTG RIVLLNLKST WIDMIEGFME
410 420 430 440 450
EAKWFNGGSA PKLEEYIENG VSTAGAYMAF AHIFFLIGEG VTHQNSQLFT
460 470 480 490 500
QKPYPKVFSA AGRILRLWDD LGTAKEEQER GDLASCVQLF MKEKSLTEEE
510 520 530 540 550
ARSRILEEIK GLWRDLNGEL VYNKNLPLSI IKVALNMARA SQVVYKHDQD
560
TYFSSVDNYV DALFFTQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY362553 mRNA Translation: AAR11765.1 |
Genome annotation databases
KEGGi | ag:AAR11765 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY362553 mRNA Translation: AAR11765.1 |
3D structure databases
AlphaFoldDBi | Q6USK1 |
SMRi | Q6USK1 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAR11765 |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
BRENDAi | 3.1.7.11, 4385 |
SABIO-RKi | Q6USK1 |
Family and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GERS_OCIBA | |
Accessioni | Q6USK1Primary (citable) accession number: Q6USK1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families