UniProtKB - Q6U736 (OPN5_HUMAN)
Protein
Opsin-5
Gene
OPN5
Organism
Homo sapiens (Human)
Status
Functioni
G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (By similarity). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (By similarity). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (By similarity). Acts as a circadian photoreceptor in the outer ear, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (By similarity). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (By similarity). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (By similarity).By similarity1 Publication
GO - Molecular functioni
- 11-cis retinal binding Source: UniProtKB
- G protein-coupled photoreceptor activity Source: UniProtKB
GO - Biological processi
- cellular response to light stimulus Source: UniProtKB
- cellular response to UV-A Source: UniProtKB
- entrainment of circadian clock by photoperiod Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: GO_Central
- hyaloid vascular plexus regression Source: UniProtKB
- phototransduction Source: UniProtKB
- phototransduction, UV Source: UniProtKB
- protein-chromophore linkage Source: UniProtKB-KW
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer |
Biological process | Sensory transduction, Vision |
Ligand | Chromophore |
Enzyme and pathway databases
PathwayCommonsi | Q6U736 |
Reactomei | R-HSA-418594, G alpha (i) signalling events R-HSA-419771, Opsins |
Names & Taxonomyi
Protein namesi | Recommended name: Opsin-5Alternative name(s): G-protein coupled receptor 136 G-protein coupled receptor PGR12 Neuropsin Transmembrane protein 13 |
Gene namesi | Name:OPN5 Synonyms:GPR136, PGR12, TMEM13 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19992, OPN5 |
MIMi | 609042, gene |
neXtProti | NX_Q6U736 |
VEuPathDBi | HostDB:ENSG00000124818.14 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | ExtracellularSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 74 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 75 – 95 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 96 – 108 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 109 – 129 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 130 – 150 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 151 – 171 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 172 – 197 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 198 – 218 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 219 – 252 | CytoplasmicSequence analysisAdd BLAST | 34 | |
Transmembranei | 253 – 273 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 274 – 288 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 289 – 309 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 310 – 353 | CytoplasmicSequence analysisAdd BLAST | 44 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 221391 |
OpenTargetsi | ENSG00000124818 |
PharmGKBi | PA134942887 |
Miscellaneous databases
Pharosi | Q6U736, Tbio |
Genetic variation databases
BioMutai | OPN5 |
DMDMi | 116242690 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197817 | 1 – 354 | Opsin-5Add BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 4 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 106 ↔ 183 | PROSITE-ProRule annotation | ||
Modified residuei | 296 | N6-(retinylidene)lysine | 1 | |
Lipidationi | 315 | S-palmitoyl cysteineSequence analysis | 1 | |
Lipidationi | 316 | S-palmitoyl cysteineSequence analysis | 1 |
Post-translational modificationi
It is uncertain whether Cys-315 or Cys-316 is palmitoylated.Curated
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | Q6U736 |
PRIDEi | Q6U736 |
ProteomicsDBi | 67396 |
PTM databases
GlyGeni | Q6U736, 1 site |
iPTMneti | Q6U736 |
PhosphoSitePlusi | Q6U736 |
Expressioni
Tissue specificityi
Detected in brain and retina and cell lines derived from neural retina.1 Publication
Gene expression databases
Bgeei | ENSG00000124818, Expressed in testis and 42 other tissues |
ExpressionAtlasi | Q6U736, baseline and differential |
Genevisiblei | Q6U736, HS |
Organism-specific databases
HPAi | ENSG00000124818, Tissue enriched (testis) |
Interactioni
Protein-protein interaction databases
STRINGi | 9606.ENSP00000360255 |
Miscellaneous databases
RNActi | Q6U736, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222330 |
InParanoidi | Q6U736 |
OrthoDBi | 704940at2759 |
PhylomeDBi | Q6U736 |
TreeFami | TF324998 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR002962, Peropsin IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01244, PEROPSIN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q6U736-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALNHTALPQ DERLPHYLRD GDPFASKLSW EADLVAGFYL TIIGILSTFG
60 70 80 90 100
NGYVLYMSSR RKKKLRPAEI MTINLAVCDL GISVVGKPFT IISCFCHRWV
110 120 130 140 150
FGWIGCRWYG WAGFFFGCGS LITMTAVSLD RYLKICYLSY GVWLKRKHAY
160 170 180 190 200
ICLAAIWAYA SFWTTMPLVG LGDYVPEPFG TSCTLDWWLA QASVGGQVFI
210 220 230 240 250
LNILFFCLLL PTAVIVFSYV KIIAKVKSSS KEVAHFDSRI HSSHVLEMKL
260 270 280 290 300
TKVAMLICAG FLIAWIPYAV VSVWSAFGRP DSIPIQLSVV PTLLAKSAAM
310 320 330 340 350
YNPIIYQVID YKFACCQTGG LKATKKKSLE GFRLHTVTTV RKSSAVLEIH
EEWE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RDV4 | D6RDV4_HUMAN | Opsin-5 | OPN5 | 382 | Annotation score: | ||
J3KPQ2 | J3KPQ2_HUMAN | Opsin-5 | OPN5 hCG_1642475 | 353 | Annotation score: |
Sequence cautioni
The sequence CAI20454 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 – 86 | Missing in AAP72128 (PubMed:14623098).Curated | 3 | |
Sequence conflicti | 133 – 145 | Missing in AAO85127 (PubMed:12679517).CuratedAdd BLAST | 13 | |
Sequence conflicti | 353 – 354 | WE → V in AAP72128 (PubMed:14623098).Curated | 2 | |
Sequence conflicti | 353 – 354 | WE → V in CAI20454 (PubMed:14574404).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY377391 mRNA Translation: AAR21109.1 AY288419 mRNA Translation: AAP72128.1 AY255615 mRNA Translation: AAO85127.1 AL356421 Genomic DNA No translation available. AL161622 Genomic DNA Translation: CAI20454.1 Sequence problems. BC126194 mRNA Translation: AAI26195.1 BC126198 mRNA Translation: AAI26199.1 |
CCDSi | CCDS4923.1 |
RefSeqi | NP_859528.1, NM_181744.3 |
Genome annotation databases
Ensembli | ENST00000371211; ENSP00000360255; ENSG00000124818 ENST00000638973; ENSP00000491960; ENSG00000124818 |
GeneIDi | 221391 |
KEGGi | hsa:221391 |
UCSCi | uc003ozc.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY377391 mRNA Translation: AAR21109.1 AY288419 mRNA Translation: AAP72128.1 AY255615 mRNA Translation: AAO85127.1 AL356421 Genomic DNA No translation available. AL161622 Genomic DNA Translation: CAI20454.1 Sequence problems. BC126194 mRNA Translation: AAI26195.1 BC126198 mRNA Translation: AAI26199.1 |
CCDSi | CCDS4923.1 |
RefSeqi | NP_859528.1, NM_181744.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9606.ENSP00000360255 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q6U736, 1 site |
iPTMneti | Q6U736 |
PhosphoSitePlusi | Q6U736 |
Genetic variation databases
BioMutai | OPN5 |
DMDMi | 116242690 |
Proteomic databases
PaxDbi | Q6U736 |
PRIDEi | Q6U736 |
ProteomicsDBi | 67396 |
Protocols and materials databases
Antibodypediai | 16965, 220 antibodies |
DNASUi | 221391 |
Genome annotation databases
Ensembli | ENST00000371211; ENSP00000360255; ENSG00000124818 ENST00000638973; ENSP00000491960; ENSG00000124818 |
GeneIDi | 221391 |
KEGGi | hsa:221391 |
UCSCi | uc003ozc.4, human |
Organism-specific databases
CTDi | 221391 |
DisGeNETi | 221391 |
GeneCardsi | OPN5 |
HGNCi | HGNC:19992, OPN5 |
HPAi | ENSG00000124818, Tissue enriched (testis) |
MIMi | 609042, gene |
neXtProti | NX_Q6U736 |
OpenTargetsi | ENSG00000124818 |
PharmGKBi | PA134942887 |
VEuPathDBi | HostDB:ENSG00000124818.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222330 |
InParanoidi | Q6U736 |
OrthoDBi | 704940at2759 |
PhylomeDBi | Q6U736 |
TreeFami | TF324998 |
Enzyme and pathway databases
PathwayCommonsi | Q6U736 |
Reactomei | R-HSA-418594, G alpha (i) signalling events R-HSA-419771, Opsins |
Miscellaneous databases
BioGRID-ORCSi | 221391, 2 hits in 866 CRISPR screens |
ChiTaRSi | OPN5, human |
GeneWikii | OPN5 |
GenomeRNAii | 221391 |
Pharosi | Q6U736, Tbio |
PROi | PR:Q6U736 |
RNActi | Q6U736, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000124818, Expressed in testis and 42 other tissues |
ExpressionAtlasi | Q6U736, baseline and differential |
Genevisiblei | Q6U736, HS |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR002962, Peropsin IPR027430, Retinal_BS |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR01244, PEROPSIN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPN5_HUMAN | |
Accessioni | Q6U736Primary (citable) accession number: Q6U736 Secondary accession number(s): A0AV33 Q86SL5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 10, 2021 | |
This is version 136 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families