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Protein

NF-kappa-B essential modulator

Gene

Ikbkg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri382 – 412CCHC NOA-typePROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-1169091 Activation of NF-kappaB in B cells
R-RNO-168638 NOD1/2 Signaling Pathway
R-RNO-1810476 RIP-mediated NFkB activation via ZBP1
R-RNO-202424 Downstream TCR signaling
R-RNO-2871837 FCERI mediated NF-kB activation
R-RNO-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-RNO-450302 activated TAK1 mediates p38 MAPK activation
R-RNO-450321 JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
R-RNO-5357905 Regulation of TNFR1 signaling
R-RNO-5357956 TNFR1-induced NFkappaB signaling pathway
R-RNO-5607764 CLEC7A (Dectin-1) signaling
R-RNO-5684264 MAP3K8 (TPL2)-dependent MAPK1/3 activation
R-RNO-5689880 Ub-specific processing proteases
R-RNO-5689896 Ovarian tumor domain proteases
R-RNO-9020702 Interleukin-1 signaling
R-RNO-933542 TRAF6 mediated NF-kB activation
R-RNO-937039 IRAK1 recruits IKK complex
R-RNO-975144 IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B essential modulator
Short name:
NEMO
Alternative name(s):
IkB kinase-associated protein 1
Short name:
IKKAP1
Inhibitor of nuclear factor kappa-B kinase subunit gamma
Short name:
I-kappa-B kinase subunit gamma
Short name:
IKK-gamma
Short name:
IKKG
Short name:
IkB kinase subunit gamma
NF-kappa-B essential modifier
Gene namesi
Name:Ikbkg
Synonyms:Nemo
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi735223 Ikbkg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002691971 – 412NF-kappa-B essential modulatorAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31Phosphoserine; by IKKBBy similarity1
Modified residuei43Phosphoserine; by IKKBBy similarity1
Disulfide bondi54InterchainBy similarity
Modified residuei68PhosphoserineBy similarity1
Modified residuei85Phosphoserine; by ATMBy similarity1
Cross-linki111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki143Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki246Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki270Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki270Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki276Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki278Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki285Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki295Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki302Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki302Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki314Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki319Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Disulfide bondi340InterchainBy similarity
Modified residuei369Phosphoserine; by IKKBBy similarity1
Modified residuei380PhosphoserineBy similarity1
Cross-linki392Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Phosphorylation at Ser-68 attenuates aminoterminal homodimerization.By similarity
Polyubiquitinated on Lys-278 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-392 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-270 and Lys-302; promotes nuclear export. Polyubiquitinated through 'Lys-27' by TRIM23; involved in antiviral innate and inflammatory responses. Linear polyubiquitinated on Lys-111, Lys-143, Lys-226, Lys-246, Lys-270, Lys-278, Lys-285, Lys-295, Lys-302 and Lys-319; the head-to-tail polyubiquitination is mediated by the LUBAC complex and plays a key role in NF-kappa-B activation. Deubiquitinated by USP10 in a TANK-dependent and -independent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (By similarity).By similarity
Sumoylated on Lys-270 and Lys-302 with SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.By similarity
Neddylated by TRIM40, resulting in stabilization of NFKBIA and down-regulation of NF-kappa-B activity.By similarity

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6TMG5
PRIDEiQ6TMG5

PTM databases

PhosphoSitePlusiQ6TMG5

Expressioni

Gene expression databases

BgeeiENSRNOG00000060936

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Interacts with COPS3, CYLD, NALP2, TRPC4AP and LRDD. Interacts with ATM; the complex is exported from the nucleus. Interacts with TRAF6. Interacts with IKBKE. Interacts with TANK; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with ZFAND5. Interacts with TNIP1 and TNFAIP3; TNIP1 facilitates the TNFAIP3-mediated de-ubiquitination of IKBKG. Interacts with TNFAIP3; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with NLRP10. Interacts with TANK; this interaction increases in response to DNA damage (By similarity). Interacts with USP10; this interaction increases in response to DNA damage (By similarity). Interacts with ZC3H12A; this interaction increases in response to DNA damage (By similarity). Interacts with TRIM29; this interaction induces IKBKG/NEMO ubiquitination and proteolytic degradation (By similarity). Interacts with TRIM13; this interaction leads to IKBKG/NEMO ubiquitination (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi259339, 1 interactor
CORUMiQ6TMG5
STRINGi10116.ENSRNOP00000053114

Structurei

3D structure databases

ProteinModelPortaliQ6TMG5
SMRiQ6TMG5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 111Interaction with CHUK/IKBKBBy similarityAdd BLAST68
Regioni150 – 250Interaction with TANKBy similarityAdd BLAST101
Regioni242 – 343Ubiquitin-binding (UBD)Add BLAST102
Regioni246 – 358Self-associationBy similarityAdd BLAST113
Regioni249 – 412Required for interaction with TNFAIP3By similarityAdd BLAST164
Regioni315 – 336Leucine-zipperSequence analysisAdd BLAST22
Regioni375 – 412Interaction with CYLDBy similarityAdd BLAST38

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili49 – 343Sequence analysisAdd BLAST295

Domaini

The leucine-zipper domain and the CCHC NOA-type zinc-finger are essential for polyubiquitin binding and for the activation of IRF3.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri382 – 412CCHC NOA-typePROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJBJ Eukaryota
ENOG410Y1FG LUCA
GeneTreeiENSGT00530000063808
HOGENOMiHOG000293233
HOVERGENiHBG000417
InParanoidiQ6TMG5
KOiK07210
OMAiEFLMQKF
OrthoDBiEOG091G0576
PhylomeDBiQ6TMG5

Family and domain databases

InterProiView protein in InterPro
IPR032419 CC2-LZ_dom
IPR021063 NEMO_N
IPR034735 NEMO_ZF
PfamiView protein in Pfam
PF16516 CC2-LZ, 1 hit
PF11577 NEMO, 1 hit
PROSITEiView protein in PROSITE
PS51801 ZF_CCHC_NOA, 1 hit

Sequencei

Sequence statusi: Complete.

Q6TMG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRHLWKNQL SEMVQPSGGP AEDQDMLGEE SSLGKPAMLH LPSEQGTPET
60 70 80 90 100
LQRCLEENQE LRDAIRQSNQ MLRERCEELL HFQVSQREEK EFLMCKFQEA
110 120 130 140 150
RKLVERLSLE KLDLRRQREQ ALEDLEHLKK CQQQMAEDKA SVKAQVTSLL
160 170 180 190 200
GELQESQSRL EAATKERQTL EGRIRAVSEQ VRQLESEREV LQQQHSVQVD
210 220 230 240 250
QLRMQNQSVE AALRMERQAA SEEKRKLAQL QAAYHQLFQD YDSHIKSSKG
260 270 280 290 300
MQLEDLRQQL QQAEEALVAK QELIDKLKEE AEQHKIVMET VPVLKAQADI
310 320 330 340 350
YKADFQAERH AREKLVERKE LLQEQLEQLQ REFNKLKVGC HESARIEDMR
360 370 380 390 400
KRHVETSQPP LLPAPAHHSF HLALSNQRRS PPEEPPDFCC PKCQYQAPDM
410
DTLQIHVMEC IE
Length:412
Mass (Da):48,066
Last modified:July 5, 2004 - v1
Checksum:i6D5D96B08A4CCEC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY392762 mRNA Translation: AAQ94056.1
RefSeqiNP_954534.1, NM_199103.1
XP_006229646.1, XM_006229584.3
XP_006229647.1, XM_006229585.3
UniGeneiRn.214715

Genome annotation databases

EnsembliENSRNOT00000078438; ENSRNOP00000075158; ENSRNOG00000060936
GeneIDi309295
KEGGirno:309295

Similar proteinsi

Entry informationi

Entry nameiNEMO_RAT
AccessioniPrimary (citable) accession number: Q6TMG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 5, 2004
Last modified: June 20, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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