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Protein

Hydantoin racemase

Gene

hyuA

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in the asymmetric conversion of racemic 5-substituted hydantoins to the corresponding L-amino acids. Catalyzes the racemization via enolization of D- and L-5-monosubstituted hydantoins. It shows a slight preference for hydantoins with short rather than long aliphatic side chains or those with aromatic rings.1 Publication

Catalytic activityi

D-5-monosubstituted hydantoin = L-5-monosubstituted hydantoin.1 Publication

Activity regulationi

Competitively inhibited by both D- and L-5-(methylthioethyl)hydantoin. Strongly inhibited by Cu2+, Hg2+, Pb2+ and Zn2+. The activity is twofold lower in the presence of Mn2+, Co2+ and Ni2+.1 Publication

Kineticsi

Kcat is 6.42 sec(-1) for L-5-ethylhydantoin (L-EH) as substrate. Kcat is 3.24 sec(-1) for D-5-isobutylhydantoin (D-IBH) as substrate. Kcat is 2.29 sec(-1) for D-5-benzylhydantoin (D-BH) as substrate. Kcat is 2.12 sec(-1) for L-5-isobutylhydantoin (L-IBH) as substrate. Kcat is 1.94 sec(-1) for L-5-benzylhydantoin (L-BH) as substrate.1 Publication
  1. KM=3.76 mM for D-5-isobutylhydantoin (D-IBH)1 Publication
  2. KM=6.41 mM for L-5-isobutylhydantoin (L-IBH)1 Publication
  3. KM=8.3 mM for L-5-benzylhydantoin (L-BH)1 Publication
  4. KM=13.89 mM for D-5-benzylhydantoin (D-BH)1 Publication
  5. KM=17.32 mM for L-5-ethylhydantoin (L-EH)1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Temperature dependencei

    The activity is gradually lost at temperatures of more than 30 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei76Seems to be responsible for recognition and proton retrieval of D-isomers1 Publication1
    Sitei181Seems to be responsible for L-isomers recognition and racemization1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processAmino-acid biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydantoin racemase1 Publication (EC:5.1.99.51 Publication)
    Gene namesi
    Name:hyuA1 Publication
    OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri382 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi76C → A: The secondary and the tertiary structure are not significantly affected. 1 Publication1
    Mutagenesisi181C → A: The secondary and the tertiary structure are not significantly affected. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004398481 – 241Hydantoin racemaseAdd BLAST241

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ6TMG4
    SMRiQ6TMG4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HyuE racemase family.Curated

    Phylogenomic databases

    eggNOGiENOG410633P Bacteria
    COG4126 LUCA

    Family and domain databases

    InterProiView protein in InterPro
    IPR015942 Asp/Glu/hydantoin_racemase
    IPR001920 Asp/Glu_race
    PfamiView protein in Pfam
    PF01177 Asp_Glu_race, 1 hit
    SUPFAMiSSF53681 SSF53681, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q6TMG4-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MHIHLINPNS TASMTAQALE SALLVKHAHT HVSASNPTDT PASIEGGADE
    60 70 80 90 100
    AMSVPGMLAE IRQGEAQGVD AYVIACFDDP GLHAAREVAK GPVIGICQAA
    110 120 130 140 150
    VQVAMTISRR FSVITTLPRS VPIIEDLVSD YGAERHCRKV RAIDLPVLAL
    160 170 180 190 200
    EEDPQRAERL LLKEIEIAKA EDGAEAIVLG CAGMSSLCDR LQKATGVPVI
    210 220 230 240
    DGVTAAVKMA EALLGAGYAT SKVNTYAYPR IKAAAGHKVC A
    Length:241
    Mass (Da):25,285
    Last modified:November 16, 2011 - v1
    Checksum:i6A861A9ECEA13DAD
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY393697 Genomic DNA Translation: AAQ93382.1
    RefSeqiWP_004434222.1, NZ_PZMJ01000038.1

    Genome annotation databases

    PATRICifig|382.52.peg.2934

    Similar proteinsi

    Entry informationi

    Entry nameiHYDRA_RHIML
    AccessioniPrimary (citable) accession number: Q6TMG4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
    Last sequence update: November 16, 2011
    Last modified: September 12, 2018
    This is version 39 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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