UniProtKB - Q6TGS6 (SYYC_DANRE)
Protein
Tyrosine--tRNA ligase, cytoplasmic
Gene
yars1
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).By similarity
Catalytic activityi
- EC:6.1.1.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | TyrosineBy similarity | 1 | |
Binding sitei | 166 | TyrosineBy similarity | 1 | |
Binding sitei | 170 | TyrosineBy similarity | 1 | |
Binding sitei | 173 | TyrosineBy similarity | 1 | |
Binding sitei | 188 | TyrosineBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- tRNA binding Source: UniProtKB-KW
- tyrosine-tRNA ligase activity Source: GO_Central
GO - Biological processi
- cellular response to DNA damage stimulus Source: ZFIN
- tyrosyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine--tRNA ligase, cytoplasmic (EC:6.1.1.1)Alternative name(s): Tyrosyl-tRNA synthetase Short name: TyrRS |
Gene namesi | Name:yars1 Synonyms:yars |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-030425-2, yars1 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239693 | 1 – 529 | Tyrosine--tRNA ligase, cytoplasmicAdd BLAST | 529 |
Proteomic databases
PaxDbi | Q6TGS6 |
PRIDEi | Q6TGS6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 365 – 469 | tRNA-bindingPROSITE-ProRule annotationAdd BLAST | 105 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 44 – 52 | 'HIGH' regionBy similarity | 9 | |
Motifi | 222 – 226 | 'KMSKS' regionBy similarity | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG2144, Eukaryota KOG2241, Eukaryota |
InParanoidi | Q6TGS6 |
OrthoDBi | 852081at2759 |
PhylomeDBi | Q6TGS6 |
TreeFami | TF300898 |
Family and domain databases
CDDi | cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002305, aa-tRNA-synth_Ic IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR002307, Tyr-tRNA-ligase |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS50886, TRBD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q6TGS6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGEQLSPDEK FQLITRNLQE VLGEERLKEI LKERELKVYW GTATTGKPHV
60 70 80 90 100
AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVKYYEQV
110 120 130 140 150
IKAMLESIGV PLDKLKFVKG TDYQLSREYT LDVYRLSSMV TEHDAKKAGA
160 170 180 190 200
EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGVDQRK IFTLAEKYLP
210 220 230 240 250
SLGYTKRSHL MNPMVPGLTG SKMSSSEEES KIDLLDKNQD VKKKLKKAFC
260 270 280 290 300
EPGNVENNGV LSFVKHVLFP LHSEFVIKRD PKFGGDKVYT DFEEVEKDFA
310 320 330 340 350
AEQIHPGDLK ASVELALNKL LDPIRKKFES PELKKLTSSA YPEPSKNKGG
360 370 380 390 400
VKGNPKQTTD DDEVIPSRLD IRVGKVISVE KHPDADSLYL EKIDVGEEQP
410 420 430 440 450
RTVVSGLVAY ITEEQLQDRL VVLLCNLKPQ KMRGIESQAM VLCASIEGEP
460 470 480 490 500
RKVEPLDPPE GSAAGDRVYV EGYESGKPDD ELKPKKKVFE KLQVDLKISG
510 520
EFVAQWKEQN LMTKLGRITC KTLKGGNIS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4I9C1 | A0A0R4I9C1_DANRE | Tyrosine--tRNA ligase | yars1 yars | 529 | Annotation score: | ||
Q1LWM3 | Q1LWM3_DANRE | Tyrosine--tRNA ligase | yars1 yars | 286 | Annotation score: | ||
Q1LWM1 | Q1LWM1_DANRE | Tyrosine--tRNA ligase | yars1 yars | 228 | Annotation score: | ||
Q1LWM2 | Q1LWM2_DANRE | Tyrosine--tRNA ligase | yars1 yars | 251 | Annotation score: | ||
Q1LWM0 | Q1LWM0_DANRE | Tyrosine--tRNA ligase, cytoplasmic | yars1 yars | 87 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 351 | V → A in AAQ97863 (PubMed:15520368).Curated | 1 | |
Sequence conflicti | 351 | V → A in CAK04282 (PubMed:15520368).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY398430 mRNA Translation: AAQ97863.1 BX547927 Genomic DNA Translation: CAK04282.1 BX000986 Genomic DNA Translation: CAK04465.1 BC076558 mRNA Translation: AAH76558.1 |
RefSeqi | NP_958473.1, NM_201316.1 XP_005159655.1, XM_005159598.3 |
Genome annotation databases
GeneIDi | 368235 |
KEGGi | dre:368235 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY398430 mRNA Translation: AAQ97863.1 BX547927 Genomic DNA Translation: CAK04282.1 BX000986 Genomic DNA Translation: CAK04465.1 BC076558 mRNA Translation: AAH76558.1 |
RefSeqi | NP_958473.1, NM_201316.1 XP_005159655.1, XM_005159598.3 |
3D structure databases
SMRi | Q6TGS6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000108800 |
Proteomic databases
PaxDbi | Q6TGS6 |
PRIDEi | Q6TGS6 |
Genome annotation databases
GeneIDi | 368235 |
KEGGi | dre:368235 |
Organism-specific databases
CTDi | 8565 |
ZFINi | ZDB-GENE-030425-2, yars1 |
Phylogenomic databases
eggNOGi | KOG2144, Eukaryota KOG2241, Eukaryota |
InParanoidi | Q6TGS6 |
OrthoDBi | 852081at2759 |
PhylomeDBi | Q6TGS6 |
TreeFami | TF300898 |
Miscellaneous databases
PROi | PR:Q6TGS6 |
Family and domain databases
CDDi | cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002305, aa-tRNA-synth_Ic IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR002307, Tyr-tRNA-ligase |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS50886, TRBD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYYC_DANRE | |
Accessioni | Q6TGS6Primary (citable) accession number: Q6TGS6 Secondary accession number(s): Q6DFZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | June 27, 2006 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries