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UniProtKB - Q6TDU1 (MGMT_CANLF)
Protein
Methylated-DNA--protein-cysteine methyltransferase
Gene
MGMT
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Functioni
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Miscellaneous
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Catalytic activityi
- a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]PROSITE-ProRule annotationEC:2.1.1.63PROSITE-ProRule annotation
- a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]PROSITE-ProRule annotationEC:2.1.1.63PROSITE-ProRule annotation
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 5 | ZincBy similarity | 1 | |
Metal bindingi | 24 | ZincBy similarity | 1 | |
Metal bindingi | 29 | ZincBy similarity | 1 | |
Metal bindingi | 89 | ZincBy similarity | 1 | |
Binding sitei | 99 | DNA; via amide nitrogenBy similarity | 1 | |
Binding sitei | 118 | DNABy similarity | 1 | |
Binding sitei | 119 | DNA; via amide nitrogenBy similarity | 1 | |
Binding sitei | 127 | DNABy similarity | 1 | |
Binding sitei | 132 | DNABy similarity | 1 | |
Active sitei | 149 | Nucleophile; methyl group acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 155 | DNA; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: GO_Central
GO - Biological processi
- DNA repair Source: GO_Central
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Methyltransferase, Transferase |
Biological process | DNA damage, DNA repair |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)Alternative name(s): 6-O-methylguanine-DNA methyltransferase Short name: MGMT O-6-methylguanine-DNA-alkyltransferase |
Gene namesi | Name:MGMT |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139357 | 1 – 216 | Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q6TDU1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 192 – 216 | DisorderedSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the MGMT family.Curated
Phylogenomic databases
eggNOGi | KOG4062, Eukaryota |
InParanoidi | Q6TDU1 |
OrthoDBi | 1573162at2759 |
Family and domain databases
CDDi | cd06445, ATase, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR001497, MethylDNA_cys_MeTrfase_AS IPR014048, MethylDNA_cys_MeTrfase_DNA-bd IPR036217, MethylDNA_cys_MeTrfase_DNAb IPR008332, MethylG_MeTrfase_N IPR036631, MGMT_N_sf IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF01035, DNA_binding_1, 1 hit PF02870, Methyltransf_1N, 1 hit |
SUPFAMi | SSF46767, SSF46767, 1 hit SSF53155, SSF53155, 1 hit |
TIGRFAMsi | TIGR00589, ogt, 1 hit |
PROSITEi | View protein in PROSITE PS00374, MGMT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6TDU1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKACKMRYK TMDSPLGKIE ISGCAQGLHE IRLHDRKTPD PGPAEAPAPP
60 70 80 90 100
ELLGCPEEMT EPLVQCVAWL DAYFREPSVL PGLPLPAIHH PIFQRGSFTA
110 120 130 140 150
RVLRKLLELV KFGDTVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVVCSSGAMG NYTGGLATKE WLLAHEGRLA GKPTYPGGSY LAGTRRGALG
210
GTPSPHPAGR DGCVRE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY423553 mRNA Translation: AAR84186.1 |
RefSeqi | NP_001003376.1, NM_001003376.1 |
Genome annotation databases
GeneIDi | 442978 |
KEGGi | cfa:442978 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY423553 mRNA Translation: AAR84186.1 |
RefSeqi | NP_001003376.1, NM_001003376.1 |
3D structure databases
AlphaFoldDBi | Q6TDU1 |
SMRi | Q6TDU1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000035481 |
Proteomic databases
PaxDbi | Q6TDU1 |
Genome annotation databases
GeneIDi | 442978 |
KEGGi | cfa:442978 |
Organism-specific databases
CTDi | 4255 |
Phylogenomic databases
eggNOGi | KOG4062, Eukaryota |
InParanoidi | Q6TDU1 |
OrthoDBi | 1573162at2759 |
Family and domain databases
CDDi | cd06445, ATase, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR001497, MethylDNA_cys_MeTrfase_AS IPR014048, MethylDNA_cys_MeTrfase_DNA-bd IPR036217, MethylDNA_cys_MeTrfase_DNAb IPR008332, MethylG_MeTrfase_N IPR036631, MGMT_N_sf IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF01035, DNA_binding_1, 1 hit PF02870, Methyltransf_1N, 1 hit |
SUPFAMi | SSF46767, SSF46767, 1 hit SSF53155, SSF53155, 1 hit |
TIGRFAMsi | TIGR00589, ogt, 1 hit |
PROSITEi | View protein in PROSITE PS00374, MGMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MGMT_CANLF | |
Accessioni | Q6TDU1Primary (citable) accession number: Q6TDU1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families