UniProtKB - Q6TBX7 (LUT1_ARATH)
Carotene epsilon-monooxygenase, chloroplastic
CYP97C1
Functioni
Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for epsilon- and beta-ring hydroxylation of alpha-carotene. Has only a low activity toward the beta-rings of beta-carotene. The preferred substrate in planta is not alpha-carotene but the epsilon-ring of zeinoxanthin (PubMed:12782726, PubMed:16890225, PubMed:19147649, PubMed:19939422).
Possesses a major beta-carotene hydroxylase activity in planta when depleted in its preferred substrate alpha-carotene (PubMed:22513258).
5 PublicationsCatalytic activityi
- α-carotene + O2 + reduced [NADPH—hemoprotein reductase] = α-cryptoxanthin + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.158
- O2 + reduced [NADPH—hemoprotein reductase] + zeinoxanthin = H+ + H2O + lutein + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.158
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 487 | Iron (heme axial ligand)1 Publication | 1 |
GO - Molecular functioni
- heme binding Source: UniProtKB
- iron ion binding Source: InterPro
- zeinoxanthin epsilon hydroxylase activity Source: TAIR
GO - Biological processi
- carotenoid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Carotenoid biosynthesis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT3G53130-MONOMER |
BRENDAi | 1.14.14.158, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Carotene epsilon-monooxygenase, chloroplastic (EC:1.14.14.158)Alternative name(s): Cytochrome P450 97C1 Protein LUTEIN DEFICIENT 1 |
Gene namesi | Name:CYP97C1 Synonyms:LUT1 Ordered Locus Names:At3g53130 ORF Names:T4D2.60 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G53130 |
TAIRi | locus:2102023, AT3G53130 |
Subcellular locationi
Chloroplast
- chloroplast Sequence analysis
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 36 | ChloroplastSequence analysisAdd BLAST | 36 | |
ChainiPRO_0000412810 | 37 – 539 | Carotene epsilon-monooxygenase, chloroplasticAdd BLAST | 503 |
Proteomic databases
PaxDbi | Q6TBX7 |
PRIDEi | Q6TBX7 |
ProteomicsDBi | 238579 |
PTM databases
iPTMneti | Q6TBX7 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q6TBX7, baseline and differential |
Genevisiblei | Q6TBX7, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 9796, 11 interactors |
IntActi | Q6TBX7, 11 interactors |
STRINGi | 3702.AT3G53130.1 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q6TBX7 |
SMRi | Q6TBX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
HOGENOMi | CLU_001570_5_5_1 |
InParanoidi | Q6TBX7 |
OMAi | WPHPETF |
OrthoDBi | 1247045at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MESSLFSPSS SSYSSLFTAK PTRLLSPKPK FTFSIRSSIE KPKPKLETNS
60 70 80 90 100
SKSQSWVSPD WLTTLTRTLS SGKNDESGIP IANAKLDDVA DLLGGALFLP
110 120 130 140 150
LYKWMNEYGP IYRLAAGPRN FVIVSDPAIA KHVLRNYPKY AKGLVAEVSE
160 170 180 190 200
FLFGSGFAIA EGPLWTARRR AVVPSLHRRY LSVIVERVFC KCAERLVEKL
210 220 230 240 250
QPYAEDGSAV NMEAKFSQMT LDVIGLSLFN YNFDSLTTDS PVIEAVYTAL
260 270 280 290 300
KEAELRSTDL LPYWKIDALC KIVPRQVKAE KAVTLIRETV EDLIAKCKEI
310 320 330 340 350
VEREGERIND EEYVNDADPS ILRFLLASRE EVSSVQLRDD LLSMLVAGHE
360 370 380 390 400
TTGSVLTWTL YLLSKNSSAL RKAQEEVDRV LEGRNPAFED IKELKYITRC
410 420 430 440 450
INESMRLYPH PPVLIRRAQV PDILPGNYKV NTGQDIMISV YNIHRSSEVW
460 470 480 490 500
EKAEEFLPER FDIDGAIPNE TNTDFKFIPF SGGPRKCVGD QFALMEAIVA
510 520 530
LAVFLQRLNV ELVPDQTISM TTGATIHTTN GLYMKVSQR
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY424805 mRNA Translation: AAR83120.1 AL132958 Genomic DNA Translation: CAB64216.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79040.1 AY091083 mRNA Translation: AAM13903.1 Different initiation. AK220829 mRNA Translation: BAD94136.1 |
PIRi | T46159 |
RefSeqi | NP_190881.2, NM_115173.3 |
Genome annotation databases
EnsemblPlantsi | AT3G53130.1; AT3G53130.1; AT3G53130 |
GeneIDi | 824479 |
Gramenei | AT3G53130.1; AT3G53130.1; AT3G53130 |
KEGGi | ath:AT3G53130 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY424805 mRNA Translation: AAR83120.1 AL132958 Genomic DNA Translation: CAB64216.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79040.1 AY091083 mRNA Translation: AAM13903.1 Different initiation. AK220829 mRNA Translation: BAD94136.1 |
PIRi | T46159 |
RefSeqi | NP_190881.2, NM_115173.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6L8H | X-ray | 2.00 | A/B/C/D | 70-539 | [»] | |
AlphaFoldDBi | Q6TBX7 | |||||
SMRi | Q6TBX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 9796, 11 interactors |
IntActi | Q6TBX7, 11 interactors |
STRINGi | 3702.AT3G53130.1 |
PTM databases
iPTMneti | Q6TBX7 |
Proteomic databases
PaxDbi | Q6TBX7 |
PRIDEi | Q6TBX7 |
ProteomicsDBi | 238579 |
Genome annotation databases
EnsemblPlantsi | AT3G53130.1; AT3G53130.1; AT3G53130 |
GeneIDi | 824479 |
Gramenei | AT3G53130.1; AT3G53130.1; AT3G53130 |
KEGGi | ath:AT3G53130 |
Organism-specific databases
Araporti | AT3G53130 |
TAIRi | locus:2102023, AT3G53130 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
HOGENOMi | CLU_001570_5_5_1 |
InParanoidi | Q6TBX7 |
OMAi | WPHPETF |
OrthoDBi | 1247045at2759 |
Enzyme and pathway databases
BioCyci | ARA:AT3G53130-MONOMER |
BRENDAi | 1.14.14.158, 399 |
Miscellaneous databases
PROi | PR:Q6TBX7 |
Gene expression databases
ExpressionAtlasi | Q6TBX7, baseline and differential |
Genevisiblei | Q6TBX7, AT |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LUT1_ARATH | |
Accessioni | Q6TBX7Primary (citable) accession number: Q6TBX7 Secondary accession number(s): Q56ZY1, Q8RWV4, Q9SCP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 21, 2011 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families