UniProtKB - Q6T486 (RBRA_DICDI)
Protein
Probable E3 ubiquitin-protein ligase rbrA
Gene
rbrA
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Might act as an E3 ubiquitin-protein ligase. Appears to be required for normal cell-type proportioning and cell sorting during multicellular development. In addition to being necessary for a normal percentage of prestalk cells and the organization of the slug, rbrA is also necessary for spore cell viability.1 Publication
Catalytic activityi
- [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.By similarity EC:2.3.2.31
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 139 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 142 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 156 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 158 | Zinc 2; via pros nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 161 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 164 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 184 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 189 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 228 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 233 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 250 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 252 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 257 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 260 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 268 | Zinc 4; via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 273 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 300 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 303 | Zinc 5PROSITE-ProRule annotation | 1 | |
Active sitei | 313 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 318 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 325 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 330 | Zinc 6PROSITE-ProRule annotation | 1 | |
Metal bindingi | 333 | Zinc 6PROSITE-ProRule annotation | 1 | |
Metal bindingi | 340 | Zinc 6; via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 350 | Zinc 6PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 139 – 189 | RING-type 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Zinc fingeri | 208 – 273 | IBR-typePROSITE-ProRule annotationAdd BLAST | 66 | |
Zinc fingeri | 300 – 333 | RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 34 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme binding Source: GO_Central
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
- sporulation resulting in formation of a cellular spore Source: dictyBase
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DDI-5689877 Josephin domain DUBs R-DDI-983168 Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable E3 ubiquitin-protein ligase rbrA (EC:2.3.2.31By similarity)Alternative name(s): Ariadne-like ubiquitin ligase |
Gene namesi | Name:rbrA ORF Names:DDB_G0286961 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelids › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0286961 rbrA |
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: GO_Central
- ubiquitin ligase complex Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
Prestalk cell numbers are reduced in slugs and these prestalk cells do not localize to the tip of slugs. Development terminates at the finger or slug stage, and these slugs do not phototax, possibly because the tip region does not properly form.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000330478 | 1 – 520 | Probable E3 ubiquitin-protein ligase rbrAAdd BLAST | 520 |
Proteomic databases
PaxDbi | Q6T486 |
PRIDEi | Q6T486 |
Interactioni
GO - Molecular functioni
- ubiquitin conjugating enzyme binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 44689.DDB0191418 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 135 – 354 | TRIAD supradomainPROSITE-ProRule annotationAdd BLAST | 220 |
Domaini
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity
Sequence similaritiesi
Belongs to the RBR family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 139 – 189 | RING-type 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Zinc fingeri | 208 – 273 | IBR-typePROSITE-ProRule annotationAdd BLAST | 66 | |
Zinc fingeri | 300 – 333 | RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST | 34 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1815 Eukaryota ENOG410XP9Y LUCA |
InParanoidi | Q6T486 |
KOi | K11968 |
OMAi | CKCGHVF |
PhylomeDBi | Q6T486 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR031127 E3_UB_ligase_RBR IPR002867 IBR_dom IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR017907 Znf_RING_CS |
PANTHERi | PTHR11685 PTHR11685, 1 hit |
Pfami | View protein in Pfam PF01485 IBR, 2 hits |
SMARTi | View protein in SMART SM00647 IBR, 2 hits |
PROSITEi | View protein in PROSITE PS51873 TRIAD, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6T486-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDDEMYEDY DVDDDSAEES GNESLDDTEY DDAATQEFDF DENQPQRSLG
60 70 80 90 100
KLTRQKSFEV LNKDDLFSES HKIIKEVKDV LSIPSEAAVS TLLRHMKWNK
110 120 130 140 150
EKLIERYMEN PEKLCIDAGV PNVMKLNATI VEKSGNVSCL ICLEDYPPTQ
160 170 180 190 200
TFALICNHRY CLPCYKNYLE IKVSEGPECI YTPCPAPKCK VIVHQDAFKQ
210 220 230 240 250
IVSPEVFERF NNFILKSYVD DNPQVKWCPA PGCIYSIRCD RKERKEAVNC
260 270 280 290 300
KCGFQYCFNC NDYEIGDHMP CPCSQVDKWL QKASDESENV TWMLANTKKC
310 320 330 340 350
PECRSPIEKN GGCMHMTCRK NAGGCGFEFC WLCRGPWTEH GSTTGGYYNC
360 370 380 390 400
NKYDKSKAKE DDDKAHDAKT ELEAYMFYYH RYESHRNAMK IADEQRRNAH
410 420 430 440 450
LKEQQILSKF DVRSADTKFL MEATEQLLKN RRVLQYSYVY GYYLDKKSQE
460 470 480 490 500
RNLFEYLQED LEKHTNLLST QYEQSLDKLE DYQAFIKWKE QVTNYTRITK
510 520
KFLDNFVDGV AGGLVNTQIN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY437441 Genomic DNA Translation: AAR10851.1 AAFI02000092 Genomic DNA Translation: EAL63978.1 |
RefSeqi | XP_637482.1, XM_632390.1 |
Genome annotation databases
EnsemblProtistsi | EAL63978; EAL63978; DDB_G0286961 |
GeneIDi | 8625881 |
KEGGi | ddi:DDB_G0286961 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY437441 Genomic DNA Translation: AAR10851.1 AAFI02000092 Genomic DNA Translation: EAL63978.1 |
RefSeqi | XP_637482.1, XM_632390.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 44689.DDB0191418 |
Proteomic databases
PaxDbi | Q6T486 |
PRIDEi | Q6T486 |
Genome annotation databases
EnsemblProtistsi | EAL63978; EAL63978; DDB_G0286961 |
GeneIDi | 8625881 |
KEGGi | ddi:DDB_G0286961 |
Organism-specific databases
dictyBasei | DDB_G0286961 rbrA |
Phylogenomic databases
eggNOGi | KOG1815 Eukaryota ENOG410XP9Y LUCA |
InParanoidi | Q6T486 |
KOi | K11968 |
OMAi | CKCGHVF |
PhylomeDBi | Q6T486 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-DDI-5689877 Josephin domain DUBs R-DDI-983168 Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:Q6T486 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR031127 E3_UB_ligase_RBR IPR002867 IBR_dom IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR017907 Znf_RING_CS |
PANTHERi | PTHR11685 PTHR11685, 1 hit |
Pfami | View protein in Pfam PF01485 IBR, 2 hits |
SMARTi | View protein in SMART SM00647 IBR, 2 hits |
PROSITEi | View protein in PROSITE PS51873 TRIAD, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBRA_DICDI | |
Accessioni | Q6T486Primary (citable) accession number: Q6T486 Secondary accession number(s): Q54L21 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | July 5, 2004 | |
Last modified: | October 16, 2019 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways