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Protein

Mastermind-like protein 1

Gene

Maml1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processNotch signaling pathway, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-MMU-2197563 NOTCH2 intracellular domain regulates transcription
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-8941856 RUNX3 regulates NOTCH signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Mastermind-like protein 1
Short name:
Mam-1
Gene namesi
Name:Maml1Imported
Synonyms:Kiaa0200Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890504 Maml1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001294941 – 1020Mastermind-like protein 1Add BLAST1020

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphoserineBy similarity1
Modified residuei127PhosphoserineCombined sources1
Modified residuei310PhosphoserineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei367PhosphoserineBy similarity1
Modified residuei827N6-acetyllysineBy similarity1
Modified residuei1019PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6T264
PaxDbiQ6T264
PRIDEiQ6T264

PTM databases

iPTMnetiQ6T264
PhosphoSitePlusiQ6T264

Expressioni

Tissue specificityi

At E9.5, strongly expressed in the telencephalon, first branchial arch, forelimb buds and somites. By E10.5, continuously expressed in brain and spinal cord. Also expressed in first and second branchial arches and limb buds. By E11.5, expression in CNS is weak but increases in mesodermal tissues. At E14.5, detected in epithelial cells in trachea, esophagus and proximal and distal tubules of the developing lungs.1 Publication

Gene expression databases

BgeeiENSMUSG00000050567 Expressed in 253 organ(s), highest expression level in skin of back
CleanExiMM_MAML1
ExpressionAtlasiQ6T264 baseline and differential
GenevisibleiQ6T264 MM

Interactioni

Subunit structurei

Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. Forms a complex with PRAG1, NOTCH1 and MAML1, in a MAML1-dependent manner (PubMed:25038227).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222170, 5 interactors
CORUMiQ6T264
IntActiQ6T264, 1 interactor
MINTiQ6T264
STRINGi10090.ENSMUSP00000059210

Structurei

3D structure databases

ProteinModelPortaliQ6T264
SMRiQ6T264
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 97Required for interaction with NOTCH proteins1 PublicationAdd BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi66 – 102Ala-richSequence analysisAdd BLAST37
Compositional biasi642 – 651Poly-Gln10

Domaini

The C-terminal region is required for transcriptional activation.1 Publication

Sequence similaritiesi

Belongs to the mastermind family.Curated

Phylogenomic databases

eggNOGiENOG410IHPP Eukaryota
ENOG4111N9A LUCA
GeneTreeiENSGT00530000063317
HOVERGENiHBG058017
InParanoidiQ6T264
KOiK06061
OMAiSEEWMND
OrthoDBiEOG091G01SE
PhylomeDBiQ6T264
TreeFamiTF332922

Family and domain databases

InterProiView protein in InterPro
IPR019082 Neuroggenic_mastermind-like_N
PfamiView protein in Pfam
PF09596 MamL-1, 1 hit
SMARTiView protein in SMART
SM01275 MamL-1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q6T264-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLPTCPMAE FALPRHSAVM ERLRRRIELC RRHHSTCEAR YEAVSPERLE
60 70 80 90 100
LERQHTFALH QRCIQAKAKR AGKHRQPPAA ATAPVAAPAP ASAPAAARLD
110 120 130 140 150
AADGPEHGRP VAHLHDTVKR SLDSAASPQN GDQPNGYGDL FPGHKKTRRE
160 170 180 190 200
APLGVSVSAN GLPPASPLGQ PDKPSGGDTL QTAGKHSLGL DPINKKCLAD
210 220 230 240 250
SGIHLNGGSN SSEPFPLSLS KELKQEPVDD LPCMIAGAGG SVAQSNLMPD
260 270 280 290 300
LNLNEQEWKE LIEELNRSVP DEDMKDLFTE DFEEKKDPEP PGSATQTPLA
310 320 330 340 350
QDINIKTEFS PAAFEQEQLG SPQVRAGSAG QTFLGASSAP VGTDSPSLGS
360 370 380 390 400
SQTLFHTTSQ PGVDNSSPNL MPASAQAQSA QRALTSVVLP SQGPGGASEL
410 420 430 440 450
SSAHQLQQIA AKQKREQMLQ NPQQAAPAPG PGQLATWQQA GPSHSPLDVP
460 470 480 490 500
YPMEKPASPS GYKQDFTNSK LLMMPGVNKS SPRPGGPYLQ PSHSNLLSHQ
510 520 530 540 550
SPSNLNQNPV NNQGSVLDYG NTKPLSHYKA DCGQGGPGSG QNKPALMAYL
560 570 580 590 600
PQQLPHLSNE QNSLFLMKPK SGNMPFRSLV PPGQEQNPSS VPVAAPAASV
610 620 630 640 650
GTQPTVSVAS THNSSPYLSS QQQAAVMKQH QLLLDQQKQR EQQQQQLQQQ
660 670 680 690 700
QFLQRQHLLA EQEKQQFQRH LTRPPPQYQD PTQSTFPQQV GQFTGPSAAV
710 720 730 740 750
PGMNNLGPSN SSCPRVFPQP GTLMSMGPGH APVSSLPSSS GQQDRGVAQF
760 770 780 790 800
TGSQSLPQNS LYGMASGLAQ IVAQPPPQAT STHAHIPRQT NVGQNASTSA
810 820 830 840 850
AYGQNSLGSA SLSQQHSKGT LPPGLTKPQV PRVSAAMGSQ NASWQHQGMP
860 870 880 890 900
NLSSQTSGNS SVNPFTAAPS FHIQQAHLKL AGQQFSQAMP SRPMAPLSSA
910 920 930 940 950
GAAGPMLPPV SAQQRNSAPA SAPPQAAPQQ GLPGLSPSGP ELGAFGQSPT
960 970 980 990 1000
SQMSGRPGLH CAQAYPVRTM GQELPFAYSG QPGSSGLSSV AGHTDLIDSL
1010 1020
LKNRTSEEWI NELDDLLGSQ
Length:1,020
Mass (Da):107,726
Last modified:July 5, 2005 - v2
Checksum:i637132736ECD3A78
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6UY95F6UY95_MOUSE
Mastermind-like protein 1
Maml1
585Annotation score:

Sequence cautioni

The sequence AAH94599 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC39618 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC97898 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti574M → T in AAH58658 (PubMed:15489334).Curated1
Sequence conflicti643 – 646Missing in AAS07633 (PubMed:15019995).Curated4
Sequence conflicti643 – 646Missing in BAC97898 (PubMed:14621295).Curated4
Sequence conflicti675P → S in AAS07633 (PubMed:15019995).Curated1
Sequence conflicti769A → P in BAC39618 (PubMed:16141072).Curated1
Sequence conflicti769A → T in AAS07633 (PubMed:15019995).Curated1
Sequence conflicti770Q → H in BAC39618 (PubMed:16141072).Curated1
Sequence conflicti822P → L in AAS07633 (PubMed:15019995).Curated1
Sequence conflicti861S → C in AAS07633 (PubMed:15019995).Curated1
Sequence conflicti1003N → K in BAC39618 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442299 mRNA Translation: AAS07633.1
AK129088 mRNA Translation: BAC97898.1 Different initiation.
AL646002 Genomic DNA No translation available.
BC024668 mRNA Translation: AAH24668.1
BC058658 mRNA Translation: AAH58658.1
BC094599 mRNA Translation: AAH94599.2 Different initiation.
AK086146 mRNA Translation: BAC39618.1 Different initiation.
CCDSiCCDS24632.2
RefSeqiNP_780543.2, NM_175334.3
UniGeneiMm.51116

Genome annotation databases

EnsembliENSMUST00000059458; ENSMUSP00000059210; ENSMUSG00000050567
GeneIDi103806
KEGGimmu:103806
UCSCiuc007isd.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442299 mRNA Translation: AAS07633.1
AK129088 mRNA Translation: BAC97898.1 Different initiation.
AL646002 Genomic DNA No translation available.
BC024668 mRNA Translation: AAH24668.1
BC058658 mRNA Translation: AAH58658.1
BC094599 mRNA Translation: AAH94599.2 Different initiation.
AK086146 mRNA Translation: BAC39618.1 Different initiation.
CCDSiCCDS24632.2
RefSeqiNP_780543.2, NM_175334.3
UniGeneiMm.51116

3D structure databases

ProteinModelPortaliQ6T264
SMRiQ6T264
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222170, 5 interactors
CORUMiQ6T264
IntActiQ6T264, 1 interactor
MINTiQ6T264
STRINGi10090.ENSMUSP00000059210

PTM databases

iPTMnetiQ6T264
PhosphoSitePlusiQ6T264

Proteomic databases

MaxQBiQ6T264
PaxDbiQ6T264
PRIDEiQ6T264

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059458; ENSMUSP00000059210; ENSMUSG00000050567
GeneIDi103806
KEGGimmu:103806
UCSCiuc007isd.2 mouse

Organism-specific databases

CTDi9794
MGIiMGI:1890504 Maml1
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IHPP Eukaryota
ENOG4111N9A LUCA
GeneTreeiENSGT00530000063317
HOVERGENiHBG058017
InParanoidiQ6T264
KOiK06061
OMAiSEEWMND
OrthoDBiEOG091G01SE
PhylomeDBiQ6T264
TreeFamiTF332922

Enzyme and pathway databases

ReactomeiR-MMU-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-MMU-2197563 NOTCH2 intracellular domain regulates transcription
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-8941856 RUNX3 regulates NOTCH signaling

Miscellaneous databases

ChiTaRSiMaml1 mouse
PROiPR:Q6T264
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050567 Expressed in 253 organ(s), highest expression level in skin of back
CleanExiMM_MAML1
ExpressionAtlasiQ6T264 baseline and differential
GenevisibleiQ6T264 MM

Family and domain databases

InterProiView protein in InterPro
IPR019082 Neuroggenic_mastermind-like_N
PfamiView protein in Pfam
PF09596 MamL-1, 1 hit
SMARTiView protein in SMART
SM01275 MamL-1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAML1_MOUSE
AccessioniPrimary (citable) accession number: Q6T264
Secondary accession number(s): Q505D8
, Q5SUC2, Q6PDK3, Q6ZQG5, Q8BIU5, Q8R3T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: September 12, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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