UniProtKB - Q6SJQ7 (CLM1_MOUSE)
CMRF35-like molecule 1
Cd300lf
Functioni
Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:17438331).
Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells (PubMed:21865548).
Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (PubMed:26768664).
Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand (PubMed:23123064).
May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (PubMed:26124135).
Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (By similarity).
Inhibits osteoclast formation (PubMed:14662855).
Induces macrophage cell death upon engagement (PubMed:18097021).
By similarity7 Publications(Microbial infection) Acts as a functional murine norovirus (MNV) receptor that mediates binding to the cell surface and is both necessary and sufficient for viral entry and replication. Primary determinant of MNV species tropism and is sufficient to render cells permissive to infection by MNV. Can render nonmurine mammalian cells susceptible to MNV infection (PubMed:27681626, PubMed:27540007).
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 241 | Phosphatase-bindingBy similarity | 1 |
GO - Molecular functioni
- ceramide binding Source: UniProtKB
- identical protein binding Source: MGI
- interleukin-4 receptor binding Source: UniProtKB
- phosphatidylserine binding Source: UniProtKB
- transmembrane signaling receptor activity Source: GO_Central
- virus receptor activity Source: UniProtKB
GO - Biological processi
- interleukin-13-mediated signaling pathway Source: UniProtKB
- negative regulation of apoptotic cell clearance Source: UniProtKB
- negative regulation of mast cell activation Source: UniProtKB
- negative regulation of MyD88-dependent toll-like receptor signaling pathway Source: UniProtKB
- osteoclast differentiation Source: MGI
- positive regulation of apoptotic cell clearance Source: UniProtKB
- positive regulation of interleukin-4-mediated signaling pathway Source: UniProtKB
- TRIF-dependent toll-like receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Host cell receptor for virus entry, Receptor |
Biological process | Immunity |
Ligand | Lipid-binding |
Names & Taxonomyi
Protein namesi | Recommended name: CMRF35-like molecule 1Short name: CLM-1 Alternative name(s): CD300 antigen-like family member F Leukocyte mono-Ig-like receptor 3 Myeloid-associated immunoglobulin-like receptor 5 Short name: MAIR-5 Short name: MAIR-V CD_antigen: CD300f |
Gene namesi | Name:Cd300lf Synonyms:Clm1, Lmir3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442359, Cd300lf |
VEuPathDBi | HostDB:ENSMUSG00000047798 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Single-pass type I membrane protein Curated
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 193 | ExtracellularSequence analysisAdd BLAST | 174 | |
Transmembranei | 194 – 214 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 215 – 337 | CytoplasmicSequence analysisAdd BLAST | 123 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000247826 | 20 – 337 | CMRF35-like molecule 1Add BLAST | 318 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 109 | 2 Publications | ||
Disulfide bondi | 55 ↔ 63 | 2 Publications |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
MaxQBi | Q6SJQ7 |
PaxDbi | Q6SJQ7 |
PRIDEi | Q6SJQ7 |
ProteomicsDBi | 281688 [Q6SJQ7-1] 281689 [Q6SJQ7-2] 281690 [Q6SJQ7-3] |
PTM databases
iPTMneti | Q6SJQ7 |
PhosphoSitePlusi | Q6SJQ7 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000047798, Expressed in granulocyte and 176 other tissues |
ExpressionAtlasi | Q6SJQ7, baseline and differential |
Genevisiblei | Q6SJQ7, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- identical protein binding Source: MGI
- interleukin-4 receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 232945, 1 interactor |
IntActi | Q6SJQ7, 1 interactor |
STRINGi | 10090.ENSMUSP00000102171 |
Miscellaneous databases
RNActi | Q6SJQ7, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q6SJQ7 |
SMRi | Q6SJQ7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q6SJQ7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 125 | Ig-like V-typeAdd BLAST | 106 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 39 – 45 | Plays an important role in murine norovirus (MNV) binding1 Publication | 7 | |
Regioni | 248 – 270 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 318 – 337 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 248 – 264 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S7MA, Eukaryota |
GeneTreei | ENSGT00940000154332 |
InParanoidi | Q6SJQ7 |
OMAi | NQEPTYC |
PhylomeDBi | Q6SJQ7 |
TreeFami | TF334441 |
Family and domain databases
DisProti | DP02478 |
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHLSLLVPFL FWITGCCTAE DPVTGPEEVS GQEQGSLTVQ CRYTSGWKDY
60 70 80 90 100
KKYWCQGVPQ RSCKTLVETD ASEQLVKKNR VSIRDNQRDF IFTVTMEDLR
110 120 130 140 150
MSDAGIYWCG ITKGGLDPMF KVTVNIGPAI QVPITVPTMP PITSTTTIFT
160 170 180 190 200
VTTTVKETSM FPTLTSYYSD NGHGGGDSGG GEDGVGDGFL DLSVLLPVIS
210 220 230 240 250
AVLLLLLLVA SLFAWRMVRR QKKAAGPPSE QAQSLEGDLC YADLSLKQPR
260 270 280 290 300
TSPGSSWKKG SSMSSSGKDH QEEVEYVTMA PFPREEVSYA ALTLAGLGQE
310 320 330
PTYGNTGCPI THVPRTGLEE ETTEYSSIRR PLPAAMP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2A6Z2 | A2A6Z2_MOUSE | CMRF35-like molecule 1 | Cd300lf | 296 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | E → Q in AB292061 (PubMed:17438331).Curated | 1 | |
Sequence conflicti | 37 | L → V in AAM19096 (Ref. 3) Curated | 1 | |
Sequence conflicti | 56 – 65 | QGVPQRSCKT → RGAYWKSCEI in AB292061 (PubMed:17438331).Curated | 10 | |
Sequence conflicti | 71 | A → K in AB292061 (PubMed:17438331).Curated | 1 | |
Sequence conflicti | 114 – 116 | GGL → AGP in AB292061 (PubMed:17438331).Curated | 3 | |
Sequence conflicti | 123 | T → A in AB292061 (PubMed:17438331).Curated | 1 | |
Sequence conflicti | 166 | S → G in AB292061 (PubMed:17438331).Curated | 1 | |
Sequence conflicti | 337 | P → H in AB292061 (PubMed:17438331).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020066 | 129 – 135 | Missing in isoform 2. 1 Publication | 7 | |
Alternative sequenceiVSP_020067 | 212 – 265 | Missing in isoform 3. 1 PublicationAdd BLAST | 54 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY457047 mRNA Translation: AAR27938.1 AB292061 mRNA No translation available. AF251703 mRNA Translation: AAM19096.1 AL606487 Genomic DNA No translation available. BC057864 mRNA Translation: AAH57864.1 |
CCDSi | CCDS25619.1 [Q6SJQ7-2] CCDS48980.1 [Q6SJQ7-1] |
RefSeqi | NP_001162624.1, NM_001169153.1 [Q6SJQ7-1] NP_663609.2, NM_145634.3 [Q6SJQ7-2] XP_017170042.1, XM_017314553.1 |
Genome annotation databases
Ensembli | ENSMUST00000051264; ENSMUSP00000053983; ENSMUSG00000047798 [Q6SJQ7-2] ENSMUST00000106561; ENSMUSP00000102171; ENSMUSG00000047798 [Q6SJQ7-1] |
GeneIDi | 246746 |
KEGGi | mmu:246746 |
UCSCi | uc007mgn.2, mouse [Q6SJQ7-1] uc011yhh.1, mouse [Q6SJQ7-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY457047 mRNA Translation: AAR27938.1 AB292061 mRNA No translation available. AF251703 mRNA Translation: AAM19096.1 AL606487 Genomic DNA No translation available. BC057864 mRNA Translation: AAH57864.1 |
CCDSi | CCDS25619.1 [Q6SJQ7-2] CCDS48980.1 [Q6SJQ7-1] |
RefSeqi | NP_001162624.1, NM_001169153.1 [Q6SJQ7-1] NP_663609.2, NM_145634.3 [Q6SJQ7-2] XP_017170042.1, XM_017314553.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZOX | X-ray | 2.10 | A | 20-128 | [»] | |
5FFL | X-ray | 1.60 | A | 20-128 | [»] | |
5OR7 | X-ray | 2.05 | C | 1-337 | [»] | |
6C6Q | X-ray | 2.00 | D/F | 20-128 | [»] | |
6C74 | X-ray | 1.36 | A | 20-128 | [»] | |
6E47 | X-ray | 1.95 | F/G | 20-128 | [»] | |
6E48 | X-ray | 1.80 | F/G | 20-128 | [»] | |
6H6L | X-ray | 2.50 | E/F/G/H | 20-128 | [»] | |
6H6M | X-ray | 2.38 | E/F | 20-128 | [»] | |
AlphaFoldDBi | Q6SJQ7 | |||||
SMRi | Q6SJQ7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 232945, 1 interactor |
IntActi | Q6SJQ7, 1 interactor |
STRINGi | 10090.ENSMUSP00000102171 |
PTM databases
iPTMneti | Q6SJQ7 |
PhosphoSitePlusi | Q6SJQ7 |
Proteomic databases
MaxQBi | Q6SJQ7 |
PaxDbi | Q6SJQ7 |
PRIDEi | Q6SJQ7 |
ProteomicsDBi | 281688 [Q6SJQ7-1] 281689 [Q6SJQ7-2] 281690 [Q6SJQ7-3] |
Protocols and materials databases
ABCDi | Q6SJQ7, 1 sequenced antibody |
DNASUi | 246746 |
Genome annotation databases
Ensembli | ENSMUST00000051264; ENSMUSP00000053983; ENSMUSG00000047798 [Q6SJQ7-2] ENSMUST00000106561; ENSMUSP00000102171; ENSMUSG00000047798 [Q6SJQ7-1] |
GeneIDi | 246746 |
KEGGi | mmu:246746 |
UCSCi | uc007mgn.2, mouse [Q6SJQ7-1] uc011yhh.1, mouse [Q6SJQ7-2] |
Organism-specific databases
CTDi | 146722 |
MGIi | MGI:2442359, Cd300lf |
VEuPathDBi | HostDB:ENSMUSG00000047798 |
Phylogenomic databases
eggNOGi | ENOG502S7MA, Eukaryota |
GeneTreei | ENSGT00940000154332 |
InParanoidi | Q6SJQ7 |
OMAi | NQEPTYC |
PhylomeDBi | Q6SJQ7 |
TreeFami | TF334441 |
Miscellaneous databases
BioGRID-ORCSi | 246746, 5 hits in 73 CRISPR screens |
ChiTaRSi | Cd300lf, mouse |
EvolutionaryTracei | Q6SJQ7 |
PROi | PR:Q6SJQ7 |
RNActi | Q6SJQ7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047798, Expressed in granulocyte and 176 other tissues |
ExpressionAtlasi | Q6SJQ7, baseline and differential |
Genevisiblei | Q6SJQ7, MM |
Family and domain databases
DisProti | DP02478 |
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CLM1_MOUSE | |
Accessioni | Q6SJQ7Primary (citable) accession number: Q6SJQ7 Secondary accession number(s): A2A6Z1 Q8K4V9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | July 5, 2004 | |
Last modified: | May 25, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families