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Protein

Testis-specific serine/threonine-protein kinase 4

Gene

TSSK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which is involved in male germ cell development and in mature sperm function (By similarity). May be involved in the Cre/Creb signaling pathway (By similarity). Phosphorylates CREB1 on 'Ser-133' in vitro and can stimulate Cre/Creb pathway in cells (PubMed:15964553). Phosphorylates CREM on 'Ser-116' in vitro (By similarity). Phosphorylates ODF2 on 'Ser-95' (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Activity regulationi

Activated by phosphorylation on Thr-197.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1 Publication1
Active sitei148Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
SignaLinkiQ6SA08
SIGNORiQ6SA08

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 4Imported (EC:2.7.11.11 Publication)
Short name:
TSK-4Curated
Short name:
TSSK-4Imported
Short name:
Testis-specific kinase 4Curated
Alternative name(s):
Serine/threonine-protein kinase 22EImported
Gene namesi
Name:TSSK4Imported
Synonyms:C14orf20Imported, STK22EImported, TSSK51 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000139908.14
HGNCiHGNC:19825 TSSK4
MIMi610711 gene
neXtProtiNX_Q6SA08

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasmic vesicle, Flagellum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54K → M: Loss of kinase activity. 1 Publication1

Organism-specific databases

DisGeNETi283629
OpenTargetsiENSG00000139908
PharmGKBiPA134908318

Polymorphism and mutation databases

BioMutaiTSSK4
DMDMi62287888

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867721 – 328Testis-specific serine/threonine-protein kinase 4Add BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei197PhosphothreonineBy similarity1

Post-translational modificationi

Activated by autophosphorylation on Thr-197. ODF2 potentiates the autophosphorylation activity of TSSK4 at Thr-197.By similarity
Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6SA08
PeptideAtlasiQ6SA08
PRIDEiQ6SA08
ProteomicsDBi67353
67354 [Q6SA08-2]
67355 [Q6SA08-3]

PTM databases

iPTMnetiQ6SA08
PhosphoSitePlusiQ6SA08

Expressioni

Tissue specificityi

Expressed only in the testis.1 Publication

Gene expression databases

BgeeiENSG00000139908 Expressed in 96 organ(s), highest expression level in right testis
CleanExiHS_TSSK4
ExpressionAtlasiQ6SA08 baseline and differential
GenevisibleiQ6SA08 HS

Organism-specific databases

HPAiHPA060660
HPA077103

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with HSP90; this interaction stabilizes and activates TSSK4 (By similarity). Interacts with ODF2 (via C-terminus); this interaction promotes ODF2 phosphorylation on 'Ser-95' (By similarity). May interact with CREM (By similarity). Interacts with CREB1; this interaction facilitates phosphorylation on 'Ser-133' (PubMed:15964553).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CREB1P162205EBI-1202583,EBI-711855

Protein-protein interaction databases

BioGridi129625, 1 interactor
IntActiQ6SA08, 1 interactor
STRINGi9606.ENSP00000339179

Structurei

3D structure databases

ProteinModelPortaliQ6SA08
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOGENOMiHOG000233016
HOVERGENiHBG101110
InParanoidiQ6SA08
KOiK08811
OMAiDPWVLKF
OrthoDBiEOG091G0EZI
PhylomeDBiQ6SA08
TreeFamiTF105333

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6SA08-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM
60 70 80 90 100
VAVKIISKKK ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI
110 120 130 140 150
ILELAQGGDV LEWIQRYGAC SEPLAGKWFS QLTLGIAYLH SKSIVHRDLK
160 170 180 190 200
LENLLLDKWE NVKISDFGFA KMVPSNQPVG CSPSYRQVNC FSHLSQTYCG
210 220 230 240 250
SFAYACPEIL RGLPYNPFLS DTWSMGVILY TLVVAHLPFD DTNLKKLLRE
260 270 280 290 300
TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD SWVLKFQPEQ
310 320
PTHEIRLLEA MCQLHNTTKQ HQSLQITT
Length:328
Mass (Da):37,454
Last modified:July 5, 2004 - v1
Checksum:iC78502FF6F71754C
GO
Isoform 2 (identifier: Q6SA08-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: R → RLMPSLSAAGR

Show »
Length:338
Mass (Da):38,439
Checksum:i8E3CC56DBF1B5F78
GO
Isoform 3 (identifier: Q6SA08-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Note: No experimental confirmation available.
Show »
Length:252
Mass (Da):29,034
Checksum:i0142331DA68E06C9
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YJB1H0YJB1_HUMAN
Testis-specific serine/threonine-pr...
TSSK4
143Annotation score:
H0YJ36H0YJ36_HUMAN
Testis-specific serine/threonine-pr...
TSSK4
108Annotation score:
H7C3J1H7C3J1_HUMAN
Testis-specific serine/threonine-pr...
TSSK4
135Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115Q → R in BAC85482 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04124733H → Y1 PublicationCorresponds to variant dbSNP:rs36036137Ensembl.1
Natural variantiVAR_04124889Y → C1 PublicationCorresponds to variant dbSNP:rs34083933Ensembl.1
Natural variantiVAR_041249145V → M1 PublicationCorresponds to variant dbSNP:rs35468205Ensembl.1
Natural variantiVAR_041250196Q → R1 PublicationCorresponds to variant dbSNP:rs1270764Ensembl.1
Natural variantiVAR_041251327T → M1 PublicationCorresponds to variant dbSNP:rs35244223Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0235591 – 76Missing in isoform 3. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_023560147R → RLMPSLSAAGR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461663 mRNA Translation: AAS17971.1
AK131042 mRNA Translation: BAC85482.1
BC111088 mRNA Translation: AAI11089.1
CCDSiCCDS53890.1 [Q6SA08-2]
CCDS76662.1 [Q6SA08-3]
CCDS9618.1 [Q6SA08-1]
RefSeqiNP_001171668.1, NM_001184739.1 [Q6SA08-2]
NP_001294996.1, NM_001308067.1 [Q6SA08-3]
NP_777604.2, NM_174944.3 [Q6SA08-1]
UniGeneiHs.314432

Genome annotation databases

EnsembliENST00000287913; ENSP00000287913; ENSG00000139908 [Q6SA08-1]
ENST00000339917; ENSP00000339179; ENSG00000139908 [Q6SA08-2]
ENST00000556621; ENSP00000452054; ENSG00000139908 [Q6SA08-3]
ENST00000643387; ENSP00000495573; ENSG00000285140 [Q6SA08-1]
ENST00000645264; ENSP00000496404; ENSG00000285140 [Q6SA08-3]
ENST00000645272; ENSP00000496710; ENSG00000285140 [Q6SA08-2]
GeneIDi283629
KEGGihsa:283629
UCSCiuc001wne.4 human [Q6SA08-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY461663 mRNA Translation: AAS17971.1
AK131042 mRNA Translation: BAC85482.1
BC111088 mRNA Translation: AAI11089.1
CCDSiCCDS53890.1 [Q6SA08-2]
CCDS76662.1 [Q6SA08-3]
CCDS9618.1 [Q6SA08-1]
RefSeqiNP_001171668.1, NM_001184739.1 [Q6SA08-2]
NP_001294996.1, NM_001308067.1 [Q6SA08-3]
NP_777604.2, NM_174944.3 [Q6SA08-1]
UniGeneiHs.314432

3D structure databases

ProteinModelPortaliQ6SA08
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129625, 1 interactor
IntActiQ6SA08, 1 interactor
STRINGi9606.ENSP00000339179

PTM databases

iPTMnetiQ6SA08
PhosphoSitePlusiQ6SA08

Polymorphism and mutation databases

BioMutaiTSSK4
DMDMi62287888

Proteomic databases

PaxDbiQ6SA08
PeptideAtlasiQ6SA08
PRIDEiQ6SA08
ProteomicsDBi67353
67354 [Q6SA08-2]
67355 [Q6SA08-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000287913; ENSP00000287913; ENSG00000139908 [Q6SA08-1]
ENST00000339917; ENSP00000339179; ENSG00000139908 [Q6SA08-2]
ENST00000556621; ENSP00000452054; ENSG00000139908 [Q6SA08-3]
ENST00000643387; ENSP00000495573; ENSG00000285140 [Q6SA08-1]
ENST00000645264; ENSP00000496404; ENSG00000285140 [Q6SA08-3]
ENST00000645272; ENSP00000496710; ENSG00000285140 [Q6SA08-2]
GeneIDi283629
KEGGihsa:283629
UCSCiuc001wne.4 human [Q6SA08-1]

Organism-specific databases

CTDi283629
DisGeNETi283629
EuPathDBiHostDB:ENSG00000139908.14
GeneCardsiTSSK4
HGNCiHGNC:19825 TSSK4
HPAiHPA060660
HPA077103
MIMi610711 gene
neXtProtiNX_Q6SA08
OpenTargetsiENSG00000139908
PharmGKBiPA134908318
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOGENOMiHOG000233016
HOVERGENiHBG101110
InParanoidiQ6SA08
KOiK08811
OMAiDPWVLKF
OrthoDBiEOG091G0EZI
PhylomeDBiQ6SA08
TreeFamiTF105333

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
SignaLinkiQ6SA08
SIGNORiQ6SA08

Miscellaneous databases

GenomeRNAii283629
PROiPR:Q6SA08
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139908 Expressed in 96 organ(s), highest expression level in right testis
CleanExiHS_TSSK4
ExpressionAtlasiQ6SA08 baseline and differential
GenevisibleiQ6SA08 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK4_HUMAN
AccessioniPrimary (citable) accession number: Q6SA08
Secondary accession number(s): Q2TA60, Q6ZNM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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